Protein Alignment Eph and Epha2

DIOPT Version :10

• Sequence 1: NP_524625.3
• Gene: Eph / 43803
• FlyBaseID: FBgn0025936
• Length: 1096
• Species: Drosophila melanogaster
• Sequence 2: NP_034269.2
• Gene: Epha2 / 13836
• MGIID: 95278
• Length: 977
• Species: Mus musculus
• Alignment Length: 1057
• Identity: 404/1057 - (38%)
• Similarity: 570/1057 - (53%)
• Gaps: 147/1057 - (13%)

  Fly    80 AHADQVVLLD-TTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNNWL 143
            |...:||||| ...:..|.|..:|||     .||  :...:.:..:....|.||:|...:.:|||
Mouse    23 AQGKEVVLLDFAAMKGELGWLTHPYG-----KGW--DLMQNIMDDMPIYMYSVCNVVSGDQDNWL 80

  Fly   144 WSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA--ATREPPPWQTDSYRLI 206
            .:.::.|..|.|::||::||:|||:.|||.|.||||||:|.:.|.|.  .|.    :|...:..|
Mouse    81 RTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTN----FQKRQFTKI 141

  Fly   207 ARIAAGEGRFNQNSD-----VDINTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYYITCPAVT 265
            ..||..|  ...:||     |.:|.|.:.:. :.:||.|.||:|.|||:::|:|:|||..||.:.
Mouse   142 DTIAPDE--ITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEML 204

  Fly   266 ENFAHFNETPTGREITIIEKQ-----NGTCVDNA-EPY--ETPTYLCKGDGKWTILTGGCRCKAG 322
            ::.|.|.||..   :.:.:.|     .|||||:| .||  |.|...|..||:|.:..|.|.|:.|
Mouse   205 QSLARFPETIA---VAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEG 266

  Fly   323 YEPNYTNKTCTECPLGTFKS-PEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSP 386
            ||.  ....|..|..|.||| ...:.|..||.::..|..|:..|:|..|::|.|.|...|.|..|
Mouse   267 YEK--VEDACRACSPGFFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRP 329

  Fly   387 PAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSP----------N 441
            |:||..||.:.:. ....:.|:||       .:|..:   .||||.:.|..|.|          :
Mouse   330 PSAPNYLTAIGMG-AKVELRWTAP-------KDTGGR---QDIVYSVTCEQCWPESGECGPCEAS 383

  Fly   442 VVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLL 506
            |.|:........|.:|:::|||...||..:.|.|.||.:              |:...|...|
Mouse   384 VRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVSGL--------------VTSRSFRTAS-- 432

  Fly   507 NIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPK 571
             :.::..|....:.|...||.            |::|       |..||.....:..|||.:..|
Mouse   433 -VSINQTEPPKVRLEDRSTTS------------LSVT-------WSIPVSQQSRVWKYEVTYRKK 477

  Fly   572 VELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQ 636
            .:.::.|  ...|:.....:..|..:|.|..||:..|..|.|:.|.:...|||.:.||..
Mouse   478 GDANSYN--VRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSAN----- 535

  Fly   637 IRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKL 701
             ..:.|.:..||:.|::.....::..|.:..            |....:|.:|....:..:
Mouse   536 -MAVIGGVAVGVVLLLVLAGVGLFIHRRRRN------------LRARQSSEDVRFSKSEQL---- 583

  Fly   702 HLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNF 766
                 .||    ::|||||||||||||:.:|..||..:.:..:.:||.||||:|.:|.||....
Mouse   584 -----KPL----KTYVDPHTYEDPNQAVLKFTTEIHPSCVARQKVIGAGEFGEVYKGTLKASSG- 638

  Fly   767 VQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDT 831
            .::|.|||||||.|.:||.|.|||:||||||||.|.|:|.|:|||::..|:||||||||||:||.
Mouse   639 KKEIPVAIKTLKAGYTEKQRVDFLSEASIMGQFSHHNIIRLEGVVSKYKPMMIITEYMENGALDK 703

  Fly   832 FLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIENA 896
            |||..||:|..|||:.|||||||||.||::||||||||||||:|||:.|:||::||||||.:|:.
Mouse   704 FLREKDGEFSVLQLVGMLRGIASGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDD 768

  Fly   897 SDA-YTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEK 960
            .:| |||.|||||:||||||||::|||||||||||||:|:||||:|||||||..||.:|:|:|..
Mouse   769 PEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSYGIVMWEVMTYGERPYWELSNHEVMKAIND 833

  Fly   961 GYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLT-----TRPS---PE 1017
            |:|||.|||||.|:||||:.|||::|:.||.||.|||.||.|.|.|.||.|     .|.|   |.
Mouse   834 GFRLPTPMDCPSAIYQLMMQCWQQERSRRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPS 898

  Fly  1018 SDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQ 1082
            :.|:..:.       |.:...|||.|||.:|..||..|.....:::.:::.:.:..:|:.|.|||
Mouse   899 TSGSEGVP-------FRTVSEWLESIKMQQYTEHFMVAGYTAIEKVVQMSNEDIKRIGVRLPGHQ 956

  Fly  1083 KKILHQ----ARQLDTI 1095
            |:|.:.    ..|::|:
Mouse   957 KRIAYSLLGLKDQVNTV 973

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	67/183 (37%)
		FN3	387..476	CDD:238020	26/98 (27%)
		FN3	536..623	CDD:238020	20/86 (23%)
		EphA2_TM	640..738	CDD:464211	26/97 (27%)
		PTKc_EphR	736..1002	CDD:270629	173/266 (65%)
		SAM_1	1029..1092	CDD:425739	18/66 (27%)
Epha2	NP_034269.2	Mediates interaction with CLDN4. /evidence=ECO:0000250	1..205		70/194 (36%)
		EphR_LBD_A2	27..200	CDD:198448	67/185 (36%)
		fn3	331..425	CDD:394996	29/118 (25%)
		fn3	439..520	CDD:394996	23/101 (23%)
		EphA2_TM	<570..611	CDD:464211	20/53 (38%)
		Mediates interaction with ARHGEF16. /evidence=ECO:0000250	607..907		184/307 (60%)
		Protein Kinases, catalytic domain	608..876	CDD:473864	174/268 (65%)
		Negatively regulates interaction with ARHGEF16. /evidence=ECO:0000250	887..977		24/94 (26%)
		SAM_EPH-A2	903..972	CDD:188942	19/75 (25%)
		PDZ-binding. /evidence=ECO:0000255	975..977