DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Eph and ephb3b

DIOPT Version :9

Sequence 1:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster
Sequence 2:	NP_001352511.1	Gene:	ephb3b / 108181204	ZFINID:	ZDB-GENE-100811-1	Length:	983	Species:	Danio rerio

Alignment Length:	1039	Identity:	433/1039 - (41%)
Similarity:	591/1039 - (56%)	Gaps:	100/1039 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly    69 LLIIISIHFKLAHADQVVLLDTTREAT-LEWTRYPYGPQAQTPGWVEES-FTDFVKGINWRSYVV 131
            ||.:......|:.|.:..|:||....| |.||.:|      ..||.|.| :.|.:..|  |:|.|
Zfish     6 LLFLCGFLLPLSSAVEETLMDTKWAMTELAWTSHP------ETGWEEVSGYDDAMNPI--RTYQV 62

  Fly   132 CDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYE--FDAATRE 194
            |:|...|.||||.|.||.|....|:|:|::||:|||:..|....||||||:|.:||  .|:||..
Zfish    63 CNVRELNQNNWLRSDFIPRKDVLRVYVEMKFTVRDCNSIPNIPGSCKETFNLFYYESDSDSATAT 127

  Fly   195 PPPWQTDSYRLIARIAAGEGRFNQNSDVDINTEVKSIA-VNKKGVYFAFRDQGACISVLAVKVYY 258
            .|.|..:.|..:..||..|. |:......:||:::|.. ::|.|.|.||:|.|||:|:::|:.:|
Zfish   128 SPFWMENPYVKVDTIAPDES-FSMLESGRVNTKIRSFGPLSKAGFYLAFQDLGACMSLISVRAFY 191

  Fly   259 ITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKGDGKWTILTGGCRCKAG 322
            ..|.....|||.|.||.||.|.|.:....||||.||.....|..| |.|||:|.:..|.|.|.||
Zfish   192 KKCSTTIANFAVFPETATGAEATSLVIAPGTCVPNALEVSVPLKLYCNGDGEWMVPVGSCTCMAG 256

  Fly   323 YEPNYTNKTCTECPLGTFKSPE-VTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSP 386
            :||......|..|..|||||.: ...|:||||||..|...:..|.|.:|:||..:|.....|.:.
Zfish   257 FEPAVKETQCQACSPGTFKSKQGEGLCSPCPPNSRTSSGAASICSCRTGYYRADSDSPDSGCTTV 321

  Fly   387 PAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSP----------N 441
            |:||.|: :..|::||.|:.||.|.....          ..|::|.:.|..|.|          |
Zfish   322 PSAPRNV-ISSVNETSLILEWSEPRDQGG----------REDLLYNVICKKCLPERGTCTRCDDN 375

  Fly   442 VVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLL 506
            |..:|......|..:|:.||:..|.|:.:|.|:|.|:..:.:....
Zfish   376 VDISPRHLGLTERHVTIRNLQAHTQYSFEIQAVNGVASKSPYAPQF------------------- 421

  Fly   507 NIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRWFPK 571
                         |.:..||.....|.|..:.:...:.....|.|..|.:.:..:..||:::..|
Zfish   422 -------------ASVNITTNQAAPSAVPTVHLTGASANTMTLSWLPPEKPNGIILDYEIKYHEK 473

  Fly   572 VELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDDSVQ 636
            .:.:|| ...:..:.:.|.|.||...|.|..|||.:|..|:|.||:.....|.....:......|
Zfish   474 DQGEAI-AHTMTAQHSYARIDGLKPGTPYVVQVRARTVAGYGRYSSPTDFGTNHQADADKALQEQ 537

  Fly   637 IRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKL 701
            :..|.|::..|::|:::.::..:..:| |.::..:.:.|..|.   .|.|         |||
Zfish   538 LPLIVGSLTAGLVFIIVVVVIAIVCLR-KQRNGSESEYTEKLQ---QYKS---------PIV--- 586

  Fly   702 HLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRGRLKIPPNF 766
                 ||   ..:.|:||.||||||:||||||:|||.:.:.||.:||.||||:|||||||:|..
Zfish   587 -----TP---GMKVYIDPFTYEDPNEAIREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKLPGR- 642

  Fly   767 VQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEYMENGSLDT 831
             ::|.|||||||.|.:|:.|.|||:||||||||||||:|.|:||||:|.||||:||:||||:||:
Zfish   643 -REIIVAIKTLKAGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIVTEFMENGALDS 706

  Fly   832 FLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREIEN- 895
            |||:|||:|..:||:.||||||:||.||||||||||||||||:|||:.|:||::||||||.:|:
Zfish   707 FLRLNDGQFTVIQLVGMLRGIAAGMKYLSDMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDD 771

  Fly   896 -ASDAYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSI 958
             ....||:. |||||:|||||||||:|||||||||||||:|:||||||||||||:.||||||.::
Zfish   772 PTDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAV 836

  Fly   959 EKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHI 1023
            |:.||||.|||||.||:|||||||.|:|..||.|:.||:|||.|.|...||.....:.......:
Zfish   837 EQDYRLPPPMDCPTALHQLMLDCWVKERNLRPKFSQIVNTLDKLIRNAASLKVVTSTHSGASQPL 901

  Fly  1024 LDG-QRGQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGITLVGHQKKIL 1086
            ||. ......|.:...||:.||||||..:|..|...:...::::||:.|..:|:||.|||||||
Zfish   902 LDRCVPDYTTFTTVGDWLDAIKMSRYRDNFLNAGFASFDLVAQMTAEDLLRIGVTLAGHQKKIL 965

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	71/179 (40%)
		FN3	387..476	CDD:238020	27/98 (28%)
		FN3	536..623	CDD:238020	22/86 (26%)
		EphA2_TM	644..736	CDD:291255	28/91 (31%)
		PTKc_EphR	736..1002	CDD:270629	186/268 (69%)
		Pkinase_Tyr	741..999	CDD:285015	181/260 (70%)
		SAM_1	1033..1092	CDD:278937	24/54 (44%)
		SAM	1039..1092	CDD:197735	23/48 (48%)
ephb3b	NP_001352511.1	EphR_LBD_B3	21..193	CDD:198446	71/180 (39%)
		fn3	323..411	CDD:306538	27/98 (28%)
		fn3	434..517	CDD:306538	22/83 (27%)
		EphA2_TM	<564..613	CDD:317033	26/72 (36%)
		PTKc_EphR_B	613..881	CDD:173638	186/269 (69%)
		SAM_superfamily	907..975	CDD:326639	24/59 (41%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	1.010