Protein Alignment Hcf and KEL2

DIOPT Version :10

• Sequence 1: NP_524621.2
• Gene: Hcf / 43788
• FlyBaseID: FBgn0039904
• Length: 1500
• Species: Drosophila melanogaster
• Sequence 2: NP_011754.3
• Gene: KEL2 / 853153
• SGDID: S000003470
• Length: 882
• Species: Saccharomyces cerevisiae
• Alignment Length: 759
• Identity: 155/759 - (20%)
• Similarity: 264/759 - (34%)
• Gaps: 202/759 - (26%)

  Fly    52 DSNLTG-FRWKRVLNPTGPQPRPRHGHRAINIKELMVVFGGGNEGIVDELHVYNTVTNQWYVPV- 114
            :|..|| :.|.||.....|.||.||....|...:..:...||       ||..:...:.|.:..
Yeast    64 ESKNTGKYIWNRVKLKNSPFPRYRHSSSFIVTNDNRIFVTGG-------LHDQSVYGDVWQIAAN 121

  Fly   115 ------------LKGDVPNGCAAYGFVVEGTRMFVFGG----MIEYGKYSNELYELQATKWEWRK 163
                        :..:.|.....:...:.|....||||    :.:.|...::||......::|..
Yeast   122 ADGTSFTSKRIDIDQNTPPPRVGHASTICGNAYVVFGGDTHKLNKNGLLDDDLYLFNINSYKWTI 186

  Fly   164 MYPESPDSGLSPCPRLGHSFTMVGE-----KIFLFGGLANESDDPKNNIPKYLNDLYILDTRGVH 223
            ..|    .|..|..|.||..:::..     |::||||..:|:         |.|||.:.|.....
Yeast   187 PQP----IGRRPLGRYGHKISIIASNPMQTKLYLFGGQVDET---------YFNDLVVFDLSSFR 238

  Fly   224 SHNGKWIVPKTYGDSPPPRESHTGISFATKSNGNLNLLIYGGMSGCRL-GDLWLLETDSMTWSKP 287
            ..|..|...:..||.|||..:||.:::..|      |.::||.:...: .|.:..:.....|||.
Yeast   239 RPNSHWEFLEPVGDLPPPLTNHTMVAYDNK------LWVFGGETPKTISNDTYRYDPAQSEWSKV 297

  Fly   288 KTSGEAPLPRSLHSSTMIGNKMYVFGGWVPLVINDSKSTTEREWKCTNTLAVLDLETMTWENVTL 352
            ||:||.|.|...|:|.:..:.|.|.||         |.|..   ..:|.:..|:|.::.|  ..|
Yeast   298 KTTGEKPPPIQEHASVVYKHLMCVLGG---------KDTHN---AYSNDVYFLNLLSLKW--YKL 348

  Fly   353 DTVEENVPRARAGHCAVGIQSRLYVWSGRDGYRKAWNN----QVCCKD------LWYLEVSKPLY 407
            ..::|.:|:.|:||....:::...:..|.|....|..|    |....|      |:.|::|. |.
Yeast   349 PRMKEGIPQERSGHSLTLMKNEKLLIMGGDKTDYASPNIHDLQTSETDQGEGTLLYTLDLSS-LN 412

  Fly   408 AVKVALVRASTHA-------LELSWTATTFAAAYVLQIQKIEQPLNTSSKLLSNNIVQQGTPT-- 463
            .:...::..|.||       |...:|.:....:...:|..|..|....||:||.|.:.:|..:
Yeast   413 ELCPGIMCESLHAGESFSNSLSGGFTPSKSTESENQEIINILTPRLPDSKVLSYNDIDEGAGSYS 477

  Fly   464 -----------------SAETSGINISANRSGSALGLGVEATS-------TV------------- 491
                             ..::|.::.|.|:.....|:.|...:       ||
Yeast   478 SALDDKAFERKSDREEKKPQSSKVDSSINKESPGTGIKVSKKNFPVLRGLTVDSEEYGSSSYKDT 542

  Fly   492 -------------LKLEKESLQLSGCQPE------------------------------------ 507
                         |.|..::|:|...|.|
Yeast   543 SCQKGIPKNLFDDLNLNLQTLRLEAQQKELETARHISQLEKEVQRLMVIKEASKDSNFQTARLKN 607

  Fly   508 -----TNVQPSVNDLLQSMSQPSSPASR-ADKDPLSSGGGTT----FNLSTSVASVHPQISVISS 562
                 |.::..:|||...:....|.||: .|:..:.:.|..|    ..:...:..:..:..|:..
Yeast   608 LEIQKTFLESRINDLKNLLMVKLSQASKLCDQITIQNNGLKTCSEHVTIKRDIIDLENKCDVLKR 672

  Fly   563 TAAVTGNDTASPSGAINSILQKFRPVV-TAVRTSTTTAVSIATSTSDPLSVRVPSTMSANVVLSS 626
            ...:..|:           :||..|.: |.:..|:.....:..|.  |.|.|.||:...|.:...
Yeast   673 QNEILVNN-----------MQKITPELHTYLNESSCYLGKLLKSY--PTSARPPSSEKDNQIYEK 724

  Fly   627 SS--STLRIV----PSVTASHSLRIASSQASGNNCRSSSAINIL 664
            .|  ...:::    .:|.|...|.:.:.:.  |:.|.|...|:|
Yeast   725 DSLNKIEKVINEMHETVRAKEKLHLETQKL--NDERDSLRANLL 766

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hcf	NP_524621.2	PLN02193	<59..363	CDD:177844	79/326 (24%)
		KELCH repeat	74..115	CDD:276965	8/53 (15%)
		KELCH repeat	123..175	CDD:276965	11/55 (20%)
		KELCH repeat	178..239	CDD:276965	18/65 (28%)
		KELCH repeat	297..360	CDD:276965	14/62 (23%)
		FN3	<1307..>1381	CDD:442628
		FN3	<1307..1338	CDD:238020
		FN3	1344..1448	CDD:238020
KEL2	NP_011754.3	NanM	77..391	CDD:442289	83/353 (24%)
		KELCH repeat	142..190	CDD:276965	10/51 (20%)
		KELCH repeat	257..308	CDD:276965	16/56 (29%)
		KELCH repeat	310..353	CDD:276965	13/56 (23%)
		SMC_N	<603..872	CDD:481474	33/179 (18%)