DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PMCA and atp2b1b

DIOPT Version :9

Sequence 1:	NP_001014689.3	Gene:	PMCA / 43787	FlyBaseID:	FBgn0259214	Length:	1255	Species:	Drosophila melanogaster
Sequence 2:	NP_001129103.1	Gene:	atp2b1b / 561190	ZFINID:	ZDB-GENE-080409-1	Length:	1240	Species:	Danio rerio

Alignment Length:	1265	Identity:	753/1265 - (59%)
Similarity:	896/1265 - (70%)	Gaps:	106/1265 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 ATIDGRPAQYGISLKQLRELMEHRGREGVMKIAE-NGGIHELCKKLYTSPNEGLSGSKADEEHRR 65
            |..||   .:|.:||:||.|||.||.:|:.:|.| .|.:..||.||.:||.|||||...|...|:
Zfish    15 ANHDG---HFGCTLKELRSLMELRGTDGLQRIQECYGDVQGLCSKLKSSPIEGLSGHPDDIARRK 76

  Fly    66 ETFGSNVIPPKPPKTFLTLVWEALQDVTLIILEVAALVSLGLSFYKPADED--------APVLQE 122
            |.||.|.||||.|||||.||||||||||||||||||::|||||||||.|.:        ..|..|
Zfish    77 EEFGKNFIPPKKPKTFLQLVWEALQDVTLIILEVAAIISLGLSFYKPPDAEREHCGRAAGGVEDE 141

  Fly   123 EEEHHGWIEGLAILISVIVVVIVTAFNDYSKERQFRGLQNRIEGEHKFSVIRGGEVCQISVGDIL 187
            .|...|||||.|||:||:.||:||||||:|||:||||||:|||.|.:|:|:|||:|.||.|.:|:
Zfish   142 GEAEAGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQRFTVLRGGQVIQIPVSEIV 206

  Fly   188 VGDIAQVKYGDLLPADGCLIQSNDLKVDESSLTGESDHVKKGPDVDPMVLSGTHVMEGSGKMVVT 252
            |||||||||||||||||.|||.||||:||||||||||||||..|.||::|||||||||||||:||
Zfish   207 VGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVKKTLDKDPILLSGTHVMEGSGKMLVT 271

  Fly   253 AVGVNSQAGIIFTLLGAAVDE--------QEAEIKKMKKEAKRANKQKNLTGENDGRSQIKGSQA 309
            |||||||.||||.|||...|:        :|.|.||.:||.||..|::....:...:.:.|..:.
Zfish   272 AVGVNSQTGIIFMLLGGGDDDDDDDEEKKEERERKKREKEEKRREKEEKKKEKERKKKEQKDKKK 336

  Fly   310 PSQRETVTSEITKSESEGNHLPQSSSSGAAETGH-----KKEKSVLQAKLTKLAIQIGYAGSTIA 369
            .....||..|           |.:|..|..|...     ||||||||.||||||:|||.||..::
Zfish   337 KKDGGTVEME-----------PLNSDDGTEEEQKKAKIPKKEKSVLQGKLTKLAVQIGKAGLFMS 390

  Fly   370 VLTVIILIIQFCIKTFVIDEKPW----KNTYANNLVKHLIIGVTVLVVAVPEGLPLAVTLSLAYS 430
            .:||:||::.|.:.||.|...||    ...|....||..||||||||||||||||||||:|||||
Zfish   391 AITVLILVVLFLVDTFWIQGLPWIKDCTPIYIQFFVKFFIIGVTVLVVAVPEGLPLAVTISLAYS 455

  Fly   431 VKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQSYICEKLCKVLPTLSDIPQHVG 495
            |||||||||||||||||||||||||||||||||||.|||||||.:|..:..|.:|....||.::.
Zfish   456 VKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQVFIAGRHFKKVPEPDLIPGNIM 520

  Fly   496 NLITMGISVNSAYTSNIMAGHNPGDLPIQVGNKTECALLGFVQGLGVKYQSIRDEITEDKFTRVY 560
            ||:..||.||.||||.||:....|.||.||||||||||||||..|...||:||.|..|:|..:||
Zfish   521 NLLVTGIGVNCAYTSKIMSAEKEGGLPRQVGNKTECALLGFVTDLRKDYQAIRCEYPEEKLYKVY 585

  Fly   561 TFNSVRKSMGTVIPRPNGGYRLYTKGASEIIMKKCAFIYGHEGTLEKFTRDMQERLIREVIEPMA 625
            |||||||||.||:...:|.||:::||||||::|||..|....|..:.|....::.::.:||||||
Zfish   586 TFNSVRKSMSTVLKNSDGSYRMFSKGASEILLKKCCKILTSNGDAKHFRPTDRDDMVTQVIEPMA 650

  Fly   626 CDGLRTISVAYRDFVPGKAAINEVHIDGEPNWDDEENIMTNLTCLCVVGIEDPVRPEVPDAIRKC 690
            .:|||||.:|||||:..         ||||:|::|.:|:|.|||:|||||||||||||||||:||
Zfish   651 SEGLRTICLAYRDFLVS---------DGEPDWNNEGDILTGLTCICVVGIEDPVRPEVPDAIKKC 706

  Fly   691 QRAGITVRMVTGDNINTARSIASKCGILRPNDDFLILEGKEFNRRIRDSNGDIQQHLIDKVWPKL 755
            |||||||||||||||||||:||:|||||...||||.|||||||||||:..|:|:|..:|||||||
Zfish   707 QRAGITVRMVTGDNINTARAIATKCGILHIGDDFLCLEGKEFNRRIRNELGEIEQERLDKVWPKL 771

  Fly   756 RVLARSSPTDKYTLVKGIIDSTVSENREVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASD 820
            |||||||||||:|||||||||||.|.|:|||||||||||||||||||||||||||||||||||||
Zfish   772 RVLARSSPTDKHTLVKGIIDSTVLEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASD 836

  Fly   821 IILTDDNFSSIVKAVMWGRNVYDSIAKFLQFQLTVNVVAVIVAFIGACAVQDSPLKAVQMLWVNL 885
            |||||||||||||||||||||||||:||||||||||||||||||.|||..|||||||||||||||
Zfish   837 IILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNL 901

  Fly   886 IMDTLASLALATEFPTPDLLLRKPYGRTKPLISRTMMKNILGQALYQLIIIFGLLFVGDVILDIE 950
            ||||.||||||||.||..|||||||||.||||||||||||||.|:|||.|||.|||.|:.|.||:
Zfish   902 IMDTFASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAVYQLTIIFTLLFAGEQIFDID 966

  Fly   951 SGRGQELNAGPTQHFTIIFNTFVMMTLFNEINARKIHGQRNVIEGLLTNPIFYTIWIFTMISQVL 1015
            |||...|:|.|::|:|::|||||:|.||||||||||||:|||.||:..|.||.:|...|.|.|.:
Zfish   967 SGRNAPLHAPPSEHYTVVFNTFVLMQLFNEINARKIHGERNVFEGIFNNIIFCSILFGTFIIQFV 1031

  Fly  1016 IIQYGKMAFSTKALTLDQWLWCIFFGIGTLVWGQLITSVPTRKLPKILSWGRGHPEEYTDGMNLG 1080
            |:|:|...||...|:::|||||:|.|.|:|:|||:|::||||:|..:::.|.|..:|......|.
Zfish  1032 IVQFGGKPFSCVGLSVEQWLWCVFLGFGSLLWGQVISTVPTRRLKFLITMGHGTKKEEIPEDELE 1096

  Fly  1081 E-ERFDSID-SDKKPRAGQILWIRGLTRLQTQLRVIRAFRSTL---EDLNERRSMHSLHSLRSPR 1140
            | |..|.|| ::.:.|.||:||.|||.|:|||:||:.|||.::   :.|....|..|:|:..|
Zfish  1097 EMEDLDEIDHAEMELRRGQVLWCRGLNRIQTQIRVVNAFRDSMSPYDGLETPESRSSIHNFMS-- 1159

  Fly  1141 TGIPVAVGGGHPLYNFNLLNPNYIKHQQQQEPKLPPQTQLNVSSGMMSPDISYIDE-DPQQQQRT 1204
                      ||.:..           :..||.:|                 .||: ||::...|
Zfish  1160 ----------HPEFRI-----------EDSEPAIP-----------------LIDDTDPEEDAPT 1186

  Fly  1205 LHNGLTSQRSSMPSNVP----SSNQSFSKI 1230
                   :|::.||.:|    |.||:.:.|
Zfish  1187 -------KRNAAPSPLPLVLSSPNQNNNAI 1209

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PMCA	NP_001014689.3	ATPase-IIB_Ca	1..1057	CDD:273668	701/1080 (65%)
		Cation_ATPase_N	36..104	CDD:279080	45/67 (67%)
		E1-E2_ATPase	172..448	CDD:278548	170/292 (58%)
		Cation_ATPase	505..598	CDD:289987	57/92 (62%)
		HAD_like	676..807	CDD:119389	111/130 (85%)
		Cation_ATPase_C	873..1051	CDD:279079	120/177 (68%)
		ATP_Ca_trans_C	1096..1142	CDD:289209	21/48 (44%)
atp2b1b	NP_001129103.1	ATPase-IIB_Ca	12..1073	CDD:273668	701/1080 (65%)
		Cation_ATPase_N	45..115	CDD:279080	46/69 (67%)
		E1-E2_ATPase	190..473	CDD:278548	170/293 (58%)
		Cation_ATPase	534..623	CDD:289987	54/88 (61%)
		HAD	<696..815	CDD:289480	99/118 (84%)
		Cation_ATPase_C	889..1067	CDD:279079	120/177 (68%)
		ATP_Ca_trans_C	1114..1163	CDD:289209	23/60 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	257	1.000	Domainoid score	I1973
eggNOG	1	0.900	-	-		E2759_KOG0204
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1385	1.000	Inparanoid score	I123
OMA	1	1.010	-	-		QHG53587
OrthoDB	1	1.010	-	-		D115892at2759
OrthoFinder	1	1.000	-	-		FOG0000362
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100143
Panther	1	1.100	-	-	O	PTHR24093
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X220
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	12	11.840