DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PMCA and atp2b3a

DIOPT Version :9

Sequence 1:	NP_001014689.3	Gene:	PMCA / 43787	FlyBaseID:	FBgn0259214	Length:	1255	Species:	Drosophila melanogaster
Sequence 2:	XP_005166875.1	Gene:	atp2b3a / 436745	ZFINID:	ZDB-GENE-040718-174	Length:	1299	Species:	Danio rerio

Alignment Length:	1255	Identity:	750/1255 - (59%)
Similarity:	903/1255 - (71%)	Gaps:	109/1255 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 GRPAQYGISLKQLRELMEHRGREGVMKIAEN-GGIHELCKKLYTSPNEGLSGSKADEEHRRETFG 69
            |....:|:|:.:|..|||.||.|.:.||.|| .....||.:|.|||.:|||.:.||.|.||:.||
Zfish    22 GHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFG 86

  Fly    70 SNVIPPKPPKTFLTLVWEALQDVTLIILEVAALVSLGLSFYKP--ADEDAPV-LQEEEEHHG--- 128
            .|.||||.|||||.||||||||:||||||:||::|||||||:|  .|.:|.| :.|..|..|
Zfish    87 MNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEAD 151

  Fly   129 --WIEGLAILISVIVVVIVTAFNDYSKERQFRGLQNRIEGEHKFSVIRGGEVCQISVGDILVGDI 191
              ||||.|||:|||.||:||||||:|||:||||||:|||.|.:|:|:|...|.||.|.:::||||
Zfish   152 ANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDI 216

  Fly   192 AQVKYGDLLPADGCLIQSNDLKVDESSLTGESDHVKKGPDVDPMVLSGTHVMEGSGKMVVTAVGV 256
            |||||||||||||.|||.||||:||||||||||||:|..|.|||:|||||||||||||:||||||
Zfish   217 AQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGV 281

  Fly   257 NSQAGIIFTLLGAAVDEQEAEIKKMKKEAK----------RANKQKNLTGENDGRSQIKGSQAPS 311
            |||.|||||||||.  |.|.|.|..|||..          .|.:...:.|:.||..:...::|..
Zfish   282 NSQTGIIFTLLGAG--EMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKK 344

  Fly   312 QRETVTSEITKSES-EGNHLPQSSSSGAAETGHKKEKSVLQAKLTKLAIQIGYAGSTIAVLTVII 375
            |.|.|..|:...:| ||..:.:.....|:..  ||||||||.||||||:|||.||..::.:||||
Zfish   345 QDEAVAMEMQPLKSAEGGEVEEKEKKKASVP--KKEKSVLQGKLTKLAVQIGKAGLVMSAITVII 407

  Fly   376 LIIQFCIKTFVIDEKPWKN----TYANNLVKHLIIGVTVLVVAVPEGLPLAVTLSLAYSVKKMMK 436
            |::.|.||||::.::||..    .|....||..||||||||||||||||||||:|||||||||||
Zfish   408 LMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMK 472

  Fly   437 DNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQSYICEKLCKVLPTLSDIPQHVGNLITMG 501
            |||||||||||||||||||||||||||||||||||||.||.::|.:.:||...|......||:..
Zfish   473 DNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSA 537

  Fly   502 ISVNSAYTSNIMAGHNPGDLPIQVGNKTECALLGFVQGLGVKYQSIRDEITEDKFTRVYTFNSVR 566
            |:||.||||.||.....|.||.||||||||||||.|..|...||::|::|.|:...:||||||||
Zfish   538 IAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVR 602

  Fly   567 KSMGTVIPRPNGGYRLYTKGASEIIMKKCAFIYGHEGTLEKFTRDMQERLIREVIEPMACDGLRT 631
            |||.|||..|:|.:|||:||||||::|||:||...:|....|....::.::::||||||||||||
Zfish   603 KSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRT 667

  Fly   632 ISVAYRDFVPGKAAINEVHIDGEPNWDDEENIMTNLTCLCVVGIEDPVRPEVPDAIRKCQRAGIT 696
            |.:|||          |:..|..|:||:|.:|::||||:.|||||||||||||||||||||||||
Zfish   668 ICIAYR----------ELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGIT 722

  Fly   697 VRMVTGDNINTARSIASKCGILRPNDDFLILEGKEFNRRIRDSNGDIQQHLIDKVWPKLRVLARS 761
            |||||||||||||:||:||||:.|.||||.:|||:||||||:..|:|:|..|||:||||||||||
Zfish   723 VRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARS 787

  Fly   762 SPTDKYTLVKGIIDSTVSENREVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDD 826
            |||||:|||||||||||.|.|:|||||||||||||||||||||||||||||||||||||||||||
Zfish   788 SPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDD 852

  Fly   827 NFSSIVKAVMWGRNVYDSIAKFLQFQLTVNVVAVIVAFIGACAVQDSPLKAVQMLWVNLIMDTLA 891
            |||||||||||||||||||:||||||||||||||||||.|||..|||||||||||||||||||.|
Zfish   853 NFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFA 917

  Fly   892 SLALATEFPTPDLLLRKPYGRTKPLISRTMMKNILGQALYQLIIIFGLLFVGDVILDIESGRGQE 956
            |||||||.||..|||||||||..|||||||||||||.|:|||:|||.|||.|:.|.||:|||...
Zfish   918 SLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAP 982

  Fly   957 LNAGPTQHFTIIFNTFVMMTLFNEINARKIHGQRNVIEGLLTNPIFYTIWIFTMISQVLIIQYGK 1021
            |::.|::|:||||||||:|.||||||||||||:|||.:|:.:||||.:|.:.|...|::|:|:|.
Zfish   983 LHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGG 1047

  Fly  1022 MAFSTKALTLDQWLWCIFFGIGTLVWGQLITSVPTRKLPKILSWGRG-HPEEYTDGMNLGEERFD 1085
            ..||...|.::|||||:|.|:|.|:|||||:||||.:|..:...|.| .|:|..| .:|.|:. :
Zfish  1048 KPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLD-EDLAEDE-E 1110

  Fly  1086 SID-SDKKPRAGQILWIRGLTRLQTQ-----------------------------LRVIRAFRST 1120
            .|| ::::.|.|||||.|||.|:|||                             :||::||||:
Zfish  1111 EIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDIRVVKAFRSS 1175

  Fly  1121 LEDLNER-RSMHSLHSLRSPRTGIPVAVGGGHP-------LYNFNLL------------NPNYIK 1165
            |.|..|| .|.:|:|..::            ||       ::|..|:            |.|:::
Zfish  1176 LYDGIERPESRNSIHDFQA------------HPEFIITDSVHNIPLIDETDVDDESERSNHNHVR 1228

  Fly  1166 ------HQQQQEPKLPPQTQ 1179
                  |.|.|.|:.||:::
Zfish  1229 VALRHPHSQPQAPQRPPRSR 1248

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PMCA	NP_001014689.3	ATPase-IIB_Ca	1..1057	CDD:273668	701/1074 (65%)
		Cation_ATPase_N	36..104	CDD:279080	43/68 (63%)
		E1-E2_ATPase	172..448	CDD:278548	173/290 (60%)
		Cation_ATPase	505..598	CDD:289987	57/92 (62%)
		HAD_like	676..807	CDD:119389	110/130 (85%)
		Cation_ATPase_C	873..1051	CDD:279079	119/177 (67%)
		ATP_Ca_trans_C	1096..1142	CDD:289209	25/75 (33%)
atp2b3a	XP_005166875.1	ATPase-IIB_Ca	23..1082	CDD:273668	699/1072 (65%)
		Cation_ATPase_N	57..116	CDD:279080	39/58 (67%)
		E1-E2_ATPase	164..484	CDD:278548	197/323 (61%)
		Cation_ATPase	545..634	CDD:289987	54/88 (61%)
		HAD	627..825	CDD:289480	140/207 (68%)
		Cation_ATPase_C	899..1077	CDD:279079	119/177 (67%)
		ATP_Ca_trans_C	1122..1198	CDD:289209	27/87 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	257	1.000	Domainoid score	I1973
eggNOG	1	0.900	-	-		E2759_KOG0204
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H123775
Inparanoid	1	1.050	1385	1.000	Inparanoid score	I123
OMA	1	1.010	-	-		QHG53587
OrthoDB	1	1.010	-	-		D115892at2759
OrthoFinder	1	1.000	-	-		FOG0000362
OrthoInspector	1	1.000	-	-		otm25312
orthoMCL	1	0.900	-	-		OOG6_100143
Panther	1	1.100	-	-	O	PTHR24093
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R99
SonicParanoid	1	1.000	-	-		X220
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.870