DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ci and Gli3

DIOPT Version :9

Sequence 1:	NP_524617.3	Gene:	ci / 43767	FlyBaseID:	FBgn0004859	Length:	1397	Species:	Drosophila melanogaster
Sequence 2:	NP_032156.2	Gene:	Gli3 / 14634	MGIID:	95729	Length:	1583	Species:	Mus musculus

Alignment Length:	1691	Identity:	467/1691 - (27%)
Similarity:	643/1691 - (38%)	Gaps:	589/1691 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 SPCINQHHPTDVSSSVTVPS-----IIPTGGTSDSIKTSIQPQICNENTLLGNAGHQHNHQPQHV 97
            ||....|.|:.:|||.|.|.     |.|....:.:.::...|
Mouse   151 SPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSP----------------------- 192

  Fly    98 HNINVTGQPHDFHPAYRIPGYMEQLYSLQRTNSASSF---------HDPY--VNCASAFHLAGLG 151
                    ||.:     |..||:.:.||..:.|.|..         ..|:  |:.|..:|...|.
Mouse   193 --------PHPY-----INPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALL 244

  Fly   152 LGS----ADFLGSRGLSSLGELHNAAVAAAAAGSLASTDFHFSVDGNRRLGSPRPPGGSIRASIS 212
            .|.    ||.|.|...:..|.:|              .::..::| :.|..|||     :.|..|
Mouse   245 TGQRSPYADILPSAATAGAGAIH--------------MEYLHAMD-STRFPSPR-----LSARPS 289

  Fly   213 RKRALSSSPYSD-SFDINSMIRFSPNSLATIMNGSRGSSAASGSYGHISATALNP---------- 266
            |||.||.||.|| |||:.:|||.|||||.||:|.||.||:|||||||:||:|::|
Mouse   290 RKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAP 354

  Fly   267 -MSHVHSTRLQQIQAHLLRASAGLLNPMTPQQVAASGFSIGHMPTSASLRVNDVHPNLSDSHIQI 330
             ..|:|    |||              ::.||...|.|  ||.|..       :||        .
Mouse   355 VSLHMH----QQI--------------LSRQQSLGSAF--GHSPPL-------IHP--------A 384

  Fly   331 TTSPTVTKDVSQVPAAAFSLKNLDDAREKKGPFKDVVPEQPSSTSGGVAQVEADSASSQLSDRCY 395
            .|.|| .:.:..:|..      |:..:...||.:.   .|...||        :||.|...|..:
Mouse   385 PTFPT-QRPIPGIPTV------LNPVQVSSGPSES---SQSKPTS--------ESAVSSTGDPMH 431

  Fly   396 NNVVNNITGIPGDVKVNSRLDEYINCGSISIPS-----NEYDCANADTTDIKDEPGDFIETNCHW 455
            |.        ...:|.:..|....:.|....|.     .|    .||..:.|.||....||||||
Mouse   432 NK--------RSKIKPDEDLPSPGSRGQQEQPEGTTLVKE----EADKDESKQEPEVIYETNCHW 484

  Fly   456 RSCRIEFITQDELVKHINNDHIQTNKKAFVCRWEDCTRGEKPFKAQYMLVVHMRRHTGEKPHKCT 520
            ..|..||.|||:||.|||||||...||.|||||.||:|.:|||||||||||||||||||||||||
Mouse   485 EGCTREFDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCT 549

  Fly   521 FEGCFKAYSRLENLKTHLRSHTGEKPYTCEYPGCSKAFSNASDRAKHQNRTHSNEKPYICKAPGC 585
            ||||.||||||||||||||||||||||.||:.||:|||||||||||||||||||||||:||.|||
Mouse   550 FEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGC 614

  Fly   586 TKRYTDPSSLRKHVKTVHGAEFYANKKHKG------LPLNDANSRLQQNNSRHNLQ-----EHNI 639
            |||||||||||||||||||.|.:..||.:|      .|..|:.|..|..:.....|     :..:
Mouse   615 TKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKEL 679

  Fly   640 DSSPCSEDSHL------GKMLGTSSPSIKSESDISSSNHHLVNGVRASDSLLTYSPDDLAENLNL 698
            .::....:..|      .:...||.||...:|..||....:.|          ||...|  .|.|
Mouse   680 SNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISN----------YSNSGL--ELPL 732

  Fly   699 DDGWNCDDDVDVADLPIVLRAMVNIGNGNASASTIGGSVLARQRFRGRLQTKGINSSTIMLCNIP 763
            .||.:..|...:.:.||:        :...|.:|   :.||.|..|....||.:..  |.|..:.
Mouse   733 TDGGSVADLSAIDETPIM--------DSTISTAT---TALALQARRNPAGTKWMEH--IKLERLK 784

  Fly   764 ESNRTFGISELNQRITELKMEPGTDAEIKIPKLPNTT------IGGYTEDPLQNQTS-------- 814
            :.|..|  ..||                  |.||:..      ||..|:.. .|.:|
Mouse   785 QVNGMF--PRLN------------------PILPSKAPAVSPLIGNGTQSN-NNYSSGGPGTLLP 828

  Fly   815 FRNTVSNKQGTVSGSIQGQFRRDSQNSTASTYYGS----------MQSRRSSQSSQVSSIPTMRP 869
            .|:.:|....||..::.   ||||..||.|:.|.|          ..|||||::||...
Mouse   829 SRSDLSGVDFTVLNTLN---RRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEG------ 884

  Fly   870 NPSCNSTASFYDPISPGCSRRSSQMSNGANCNSFTSTSGLPVLNKESNKSLNACINKPNIGVQGV 934
            .|...|.|..|||||...|||||:.|.|....|..|.:  ||..........|....|
Mouse   885 RPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLT--PVQQYRLKAKYAAATGGP------- 940

  Fly   935 GIYNSSLPPPPSSHLIATNLKRLQR---------------------KDSEYHNFTSGRFSVPSYM 978
                   ||.|..|:...:||....                     .|...|.: .||..:|
Mouse   941 -------PPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCSDGGGHTY-RGRHLMP--- 994

  Fly   979 HSLHIKNNKPVGENEFDKAIASNARRQTDPVPNINLD-PLTNISRFSTTPHSFDINVGKTNNIAS 1042
            |.                |:|::.||.:|||..::.: .|..:.|||:      :|.....|:..
Mouse   995 HD----------------ALANSVRRASDPVRTVSENMSLARVQRFSS------LNSFNPPNLPP 1037

  Fly  1043 SINKDNLRKDLFT----------------------VSIKADMAMTSDQHPNERINLDEVEELILP 1085
            |:.|.:|....:|                      |:::| :.|.:|.:.|        :|.:||
Mouse  1038 SVEKRSLVLQNYTRQESSQPRYFQASPCPPSITENVALEA-LTMDADANLN--------DEDLLP 1093

  Fly  1086 DEMLQYLNL-------------VKDDT----------------NHLEKEHQAV------------ 1109
            |:::||||.             :.:|:                :|:.:|:.|:
Mouse  1094 DDVVQYLNSQNQTGYGQQLQSGISEDSKVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDL 1158

  Fly  1110 -----PVGSNVSETIASNHYR--------EQSNIYYTNKQILTPPSNV------------DIQPN 1149
                 .|.|..|: ::|:..:        :|...:.....::..|.|:            .:..|
Mouse  1159 PIQWNEVSSGTSD-LSSSKLKCGQQRPSAQQPRGFGLYNNMVVHPHNLWKVGTGPAGGYQTLGEN 1222

  Fly  1150 TTKFTVQDKFAMTA--------------------VGGSFSQRELSTLAV---------------- 1178
            ::.:...:.||:.:                    .|...:::.|::.|:
Mouse  1223 SSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQLNRQPLTSSALDHACGTGIQGSKLKGN 1287

  Fly  1179 ----------------PNE----------------HGHAKCE----SFHHQ-------------- 1193
                            |||                .|:...:    |..||
Mouse  1288 SLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYIAHQLLSGSMQHQGPSRPGQQVLGQVG 1352

  Fly  1194 SQKYMNTDIGSKQQSALPSAHQRQTEKSNYNQIIDSSMTSLPELNVDSIYPRNETE-----NIFK 1253
            :..::|...|:  :|.||......::        .|||.::......:.|..|..:     |:..
Mouse  1353 ATSHINIYQGT--ESCLPGTQDNSSQ--------PSSMAAIRGYQPCASYGGNRRQAMPRGNLTL 1407

  Fly  1254 VHG--------DHDNEIQCGIISQSQMSPSTNLNNDGQFSTVNMQPITTSKLFPPEPQK------ 1304
            ..|        ...|.|:.....|||...||..|..|||....|.       |..:.:|
Mouse  1408 QQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMG-------FSQQDRKAGSFSL 1465

  Fly  1305 --IVCDTQASNTSVMHL------------DTYQ-RTLEYVQ-----------------------S 1331
              ..|..|.:.|....|            |::: ..||.||                       .
Mouse  1466 SDANCLLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSI 1530

  Fly  1332 CQNWMETNNTSTNQIQSLPGMPVNNTLFPDVSSSTHPYHGTNMVINDMTTSLTSLLEENRYLQMM 1396
            .||...:::..|....|||        ||.:|..|     |||.|.||::.||||.||:::|.:|
Mouse  1531 IQNLSHSSSRLTTPRASLP--------FPSLSMGT-----TNMAIGDMSSLLTSLAEESKFLAVM 1582

  Fly  1397 Q 1397
            |
Mouse  1583 Q 1583

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ci	NP_524617.3	zf-H2C2_2	503..530	CDD:290200	25/26 (96%)
		C2H2 Zn finger	519..541	CDD:275368	20/21 (95%)
		zf-H2C2_2	533..560	CDD:290200	22/26 (85%)
		C2H2 Zn finger	549..572	CDD:275368	19/22 (86%)
		C2H2 Zn finger	580..601	CDD:275368	19/20 (95%)
Gli3	NP_032156.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..78
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	368..475		32/153 (21%)
		zf-H2C2_2	532..559	CDD:290200	25/26 (96%)
		C2H2 Zn finger	548..570	CDD:275368	20/21 (95%)
		zf-H2C2_2	562..589	CDD:290200	22/26 (85%)
		C2H2 Zn finger	578..601	CDD:275368	19/22 (86%)
		C2H2 Zn finger	609..630	CDD:275368	19/20 (95%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	620..728		34/117 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	809..828		4/19 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	863..918		22/60 (37%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1164..1189		4/25 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167847744
Domainoid	1	1.000	113	1.000	Domainoid score	I6115
eggNOG	1	0.900	-	-		E33208_3BFAM
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	446	1.000	Inparanoid score	I1620
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003548
OrthoInspector	1	1.000	-	-		otm42485
orthoMCL	1	0.900	-	-		OOG6_104319
Panther	1	1.100	-	-	O	PTHR45718
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R588
SonicParanoid	1	1.000	-	-		X2040
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	13	12.870