Protein Alignment Med and Smad4

DIOPT Version :10

• Sequence 1: NP_524610.1
• Gene: Med / 43725
• FlyBaseID: FBgn0288966
• Length: 771
• Species: Drosophila melanogaster
• Sequence 2: NP_062148.1
• Gene: Smad4 / 50554
• RGDID: 3033
• Length: 552
• Species: Rattus norvegicus
• Alignment Length: 757
• Identity: 384/757 - (50%)
• Similarity: 423/757 - (55%)
• Gaps: 237/757 - (31%)

  Fly    35 SNAPTSADACLSIVHSLMCHRQGGESEGFAKRAIESLVKKLKEKRDELDSLITAITTNGAHPSKC 99
            :|.|||.||||||||||||||||||||.||||||||||||||||:||||||||||||||||||||
  Rat     7 TNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKC 71

  Fly   100 VTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELKHVKYCAFAFDLKCDSVCVNPYHYERV 164
            ||||||||||||||||||||||||||:|||||||||||||||||.:|||||||||||||||||||
  Rat    72 VTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERV 136

  Fly   165 VSPGIDLSGLSLQSG--PSRLVKDEY----SAGPLVGSMDIDGNDIGTIQHHPTQMVGPGGYGYP 223
            ||||||||||:|||.  ||.||||||    ...|   |:..:|:.|.||||.|:.......|..|
  Rat   137 VSPGIDLSGLTLQSNAPPSMLVKDEYVHDFEGQP---SLPTEGHSIQTIQHPPSNRASTETYSAP 198

  Fly   224 Q--GPSEYVGDANPMSAM-FPTGRTIPKIEPQDGVAGSRGSWMVPPPPRLGQPPQQQQQQPQQQT 285
            .  .|||    :|..|.. ||
  Rat   199 ALLAPSE----SNATSTTNFP-------------------------------------------- 215

  Fly   286 PQPTQQQQAQSQAAAHSLPVPHGMPGMPGPMNPGPVMAPPPPPQQAQNPQGNGVHHTQANSPTDP 350
                            ::||.                                       |.:.|
  Rat   216 ----------------NIPVA---------------------------------------STSQP 225

  Fly   351 ASALAMQQQQQQQQQQQQQQQQQQQQSGGVPNGSVNAGGGAAAGGQYYGQPPPVSQMQGAGGGGT 415
            ||.||                           ||.:.|....|.|...||
  Rat   226 ASILA---------------------------GSHSEGLLQIASGPQPGQ--------------- 248

  Fly   416 SVAPSVHAQQNGYVSQPGSAGSAPVGGGGVFGTAQPTPQQPQQPPTGVQANTGSAGAQAGAGGGA 480
                    ||||:.:||                                         |.....:
  Rat   249 --------QQNGFTAQP-----------------------------------------ATYHHNS 264

  Fly   481 AGTWTGPNTLTYTQSM----------QPPDPRSLPGGFWNSSLSGDLGSPQQTPPQQQQQQQQPR 535
            ..||||..|..||.::          .||.|.. ||.:|              |...:...|.|
  Rat   265 TTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPH-PGHYW--------------PVHNELAFQPP- 313

  Fly   536 LLSRQPPPEYWCSIAYFELDTQVGETFKVPSAKPNVIIDGYVDPSGGNRFCLGALSNVHRTEQSE 600
             :|..|.|||||||||||:|.||||||||||:.|.|.:|||||||||:|||||.||||||||..|
  Rat   314 -ISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIE 377

  Fly   601 RARLHIGKGVQLDLRGEGDVWLRCLSDNSVFVQSYYLDREAGRTPGDAVHKIYPAACIKVFDLRQ 665
            ||||||||||||:.:||||||:|||||::||||||||||||||.||||||||||:|.||||||||
  Rat   378 RARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQ 442

  Fly   666 CHQQMHSLATNAQAAAAAQAAAVAG-VANQQMGGG---GRSMTAAAGIGVDDLRRLCILRLSFVK 726
            ||:||...|..|||||||||||||| :......||   ..|::|||||||||||||||||:||||
  Rat   443 CHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVK 507

  Fly   727 GWGPDYPRQSIKETPCWIEVHLHRALQLLDEVLHAMPIDGPR 768
            |||||||||||||||||||:||||||||||||||.|||..|:
  Rat   508 GWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQ 549

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Med	NP_524610.1	MH1_SMAD_4	42..166	CDD:199816	118/123 (96%)
		MH2_SMAD_4	543..760	CDD:199823	177/220 (80%)
Smad4	NP_062148.1	Mediates interaction with ZBTB7A. /evidence=ECO:0000250|UniProtKB:Q13485	1..322		202/528 (38%)
		MH1_SMAD_4	14..138	CDD:199816	118/123 (96%)
		Required for interaction with TSC22D1. /evidence=ECO:0000250|UniProtKB:Q13485	44..69		23/24 (96%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	167..193		9/28 (32%)
		SAD	275..320		13/61 (21%)
		MH2_SMAD_4	320..541	CDD:199823	177/220 (80%)