Protein Alignment Med and Smad4

DIOPT Version :10

• Sequence 1: NP_524610.1
• Gene: Med / 43725
• FlyBaseID: FBgn0288966
• Length: 771
• Species: Drosophila melanogaster
• Sequence 2: NP_032566.2
• Gene: Smad4 / 17128
• MGIID: 894293
• Length: 551
• Species: Mus musculus
• Alignment Length: 753
• Identity: 381/753 - (50%)
• Similarity: 423/753 - (56%)
• Gaps: 230/753 - (30%)

  Fly    35 SNAPTSADACLSIVHSLMCHRQGGESEGFAKRAIESLVKKLKEKRDELDSLITAITTNGAHPSKC 99
            :|.|||.||||||||||||||||||||.||||||||||||||||:||||||||||||||||||||
Mouse     7 TNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKC 71

  Fly   100 VTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELKHVKYCAFAFDLKCDSVCVNPYHYERV 164
            ||||||||||||||||||||||||||:|||||||||||||||||.:|||||||||||||||||||
Mouse    72 VTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERV 136

  Fly   165 VSPGIDLSGLSLQS-GPSRLVKDEY----SAGPLVGSMDIDGNDIGTIQHHPTQMVGPGGYGYPQ 224
            ||||||||||:||| .||.||||||    ...|   |:..:|:.|.||||.|:.......|..|.
Mouse   137 VSPGIDLSGLTLQSNAPSMLVKDEYVHDFEGQP---SLPTEGHSIQTIQHPPSNRASTETYSAPA 198

  Fly   225 GPSEYVGDANPMSAMFPTGRTIPKIEPQDGVAGSRGSWMVPPPPRLGQPPQQQQQQPQQQTPQPT 289
                                                  ::.|
Mouse   199 --------------------------------------LLAP----------------------- 202

  Fly   290 QQQQAQSQAAAHSLPVPHGMPGMPGPMNPGPVMAPPPPPQQAQNPQGNGVHHTQANSPTDPASAL 354
                |:|.|.:.:     ..|.:|                              ..|.:.|||.|
Mouse   203 ----AESNATSTT-----NFPNIP------------------------------VASTSQPASIL 228

  Fly   355 AMQQQQQQQQQQQQQQQQQQQQSGGVPNGSVNAGGGAAAGGQYYGQPPPVSQMQGAGGGGTSVAP 419
            |                           ||.:.|....|.|...||
Mouse   229 A---------------------------GSHSEGLLQIASGPQPGQ------------------- 247

  Fly   420 SVHAQQNGYVSQPGSAGSAPVGGGGVFGTAQPTPQQPQQPPTGVQANTGSAGAQAGAGGGAAGTW 484
                ||||:.:||                                         |.....:..||
Mouse   248 ----QQNGFTAQP-----------------------------------------ATYHHNSTTTW 267

  Fly   485 TGPNTLTYTQSM----------QPPDPRSLPGGFWNSSLSGDLGSPQQTPPQQQQQQQQPRLLSR 539
            ||..|..||.::          .||.|.. ||.:|              |...:...|.|  :|.
Mouse   268 TGSRTAPYTPNLPHHQNGHLQHHPPMPPH-PGHYW--------------PVHNELAFQPP--ISN 315

  Fly   540 QPPPEYWCSIAYFELDTQVGETFKVPSAKPNVIIDGYVDPSGGNRFCLGALSNVHRTEQSERARL 604
            .|.|||||||||||:|.||||||||||:.|.|.:|||||||||:|||||.||||||||..|||||
Mouse   316 HPAPEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARL 380

  Fly   605 HIGKGVQLDLRGEGDVWLRCLSDNSVFVQSYYLDREAGRTPGDAVHKIYPAACIKVFDLRQCHQQ 669
            ||||||||:.:||||||:|||||::||||||||||||||.||||||||||:|.||||||||||:|
Mouse   381 HIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQ 445

  Fly   670 MHSLATNAQAAAAAQAAAVAG-VANQQMGGG---GRSMTAAAGIGVDDLRRLCILRLSFVKGWGP 730
            |...|..|||||||||||||| :......||   ..|::|||||||||||||||||:||||||||
Mouse   446 MQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGP 510

  Fly   731 DYPRQSIKETPCWIEVHLHRALQLLDEVLHAMPIDGPR 768
            |||||||||||||||:||||||||||||||.|||..|:
Mouse   511 DYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQ 548

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Med	NP_524610.1	MH1_SMAD_4	42..166	CDD:199816	118/123 (96%)
		MH2_SMAD_4	543..760	CDD:199823	177/220 (80%)
Smad4	NP_032566.2	Mediates interaction with ZBTB7A. /evidence=ECO:0000250|UniProtKB:Q13485	1..321		199/524 (38%)
		MH1_SMAD_4	14..138	CDD:199816	118/123 (96%)
		Required for interaction with TSC22D1. /evidence=ECO:0000250|UniProtKB:Q13485	44..69		23/24 (96%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	166..192		9/28 (32%)
		SAD	274..319		13/61 (21%)
		MH2_SMAD_4	319..540	CDD:199823	177/220 (80%)