DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Gcn2 and EIF2AK4

DIOPT Version :9

Sequence 1:	NP_001263142.1	Gene:	Gcn2 / 43709	FlyBaseID:	FBgn0019990	Length:	1591	Species:	Drosophila melanogaster
Sequence 2:	NP_001013725.2	Gene:	EIF2AK4 / 440275	HGNCID:	19687	Length:	1649	Species:	Homo sapiens

Alignment Length:	1745	Identity:	551/1745 - (31%)
Similarity:	880/1745 - (50%)	Gaps:	267/1745 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 EKAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKL 68
            ::..||:.:||..||:.:::|:|.|.:||||.|...:.:|.:|.:.|.|  ....|.|.||...|
Human    13 DEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYP--QGLTGEEVYVKVDL 75

  Fly    69 HVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKP 133
            .|.||..||.:.|:|.|:.:||:|::.:..|:::|:..:::..|||||:|||..||:||.|||||
Human    76 RVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKP 140

  Fly   134 PKGSFYDQMLQDK-QKRDQELQDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSE-- 195
            |..||:::||:.: |:..|.|.:.:|:.|..||: ::.|::||||..|.|.||:...::...|
Human   141 PPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQRE-ILHEIQRRKEEIKEEKKRKEMAKQERLEIA 204

  Fly   196 --SNPRHPSSSESS--------ENSSPYYRGH-----------------------IYPSKCLDHR 227
              ||..|.|..:..        ...||.:.|:                       ..|..|
Human   205 SLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSC---- 265

  Fly   228 NTETLYFHKMGR----QIQRGCCVGEGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIG 288
              |.|||: ||.    .:.:|.|:| ...|.|.:.|..::...|..:.:.||.:::.:...|.:
Human   266 --EILYFN-MGSPDQLMVHKGKCIG-SDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFL- 325

  Fly   289 GGKCHWSSESKCMGSHRVDEVMASI---EKQVSSLSQLQHKNLVSYECVLCIKRKEGLLVYLVQD 350
                  :|:.|    .::|:....|   |.:.:||.:|.|.|:|.|..:...::.:.::|.::.:
Human   326 ------TSQEK----EKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVE 380

  Fly   351 FLLGTSVFS-ISSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFMDNTGNVRVSDFS 414
            .:.|.|:.: :|.|....:...|.....:|..|.:||:..|.|..|..:.|.:|..|.|:::|:|
Human   381 HISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYS 445

  Fly   415 LVPNLLE----------------------------------LLSGAGQSSSCGDLPALGALVESL 445
            :...|.:                                  ||....|...||:.|   ..:.|.
Human   446 ISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYP---VTIPSD 507

  Fly   446 MPTNSYEMRDFVDKC---NSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQR--TGS 505
            :|.   :.:||:.||   :.....|..:||:|.|:      ..|..|||.:|...::..:  ..:
Human   508 LPA---DFQDFLKKCVCLDDKERWSPQQLLKHSFI------NPQPKMPLVEQSPEDSEGQDYVET 563

  Fly   506 AMP-YQIPTLALSQ------SRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSR 563
            .:| .::|:.|...      ||...|||.|..|||||||.|:||:|.||...||:||||:...||
Human   564 VIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASR 628

  Fly   564 QLYKKMTREVELLSRLNHENVVRYFNSWIE----------------------------------S 594
            | ::::..||.|||||:|||:|||:|:|||                                  .
Human   629 Q-FRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDG 692

  Fly   595 VDDADAAEMDKLLGG--EWSQS-QQDLSVK-PAKSPQLGPTLEEDEDEEDSSSSMWNGYIPNMED 655
            :|..:||....:|..  |||.| ::..|.: ||..|  |.:.:||:||::........::| ..|
Human   693 LDSVEAAAPPPILSSSVEWSTSGERSASARFPATGP--GSSDDEDDDEDEHGGVFSQSFLP-ASD 754

  Fly   656 SDSDGI-EFVDSNGKVAVYDDE--EQEDSTRGKTNSPKPLMQVMYIQMEFCEKCTLRTAIDDNLF 717
            |:||.| :..|.|.|....|::  |:......:.:.....:..:|||||:|||.|||..||..|:
Human   755 SESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLY 819

  Fly   718 NDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAP 782
            .||.|||||||||.:|||:||::|:||||||||||||||.|.:|||||||| |..||..|.....
Human   820 RDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLA-TDHLAFSADSKQD 883

  Fly   783 APVNQ-ITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMER 846
            ..... |.|...|..||.|||.|||:||:.| ::||.||||||:::||||.|||...|..|:.||
Human   884 DQTGDLIKSDPSGHLTGMVGTALYVSPEVQG-STKSAYNQKVDLFSLGIIFFEMSYHPMVTASER 947

  Fly   847 AQTIMALRN-VSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANEL 910
            ...:..||: .|...|:. ..|.::.|...::.||||||||:||||.|||.|:|:||.|:|.:||
Human   948 IFVLNQLRDPTSPKFPED-FDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESEL 1011

  Fly   911 QEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEF 975
            .|:|.|.|.|...|||:.::|:...|.....:::||.                .||:..|..|..
Human  1012 HEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYD----------------SDILKGNFSIRT 1060

  Fly   976 VKAK------VVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQF 1034
            .|.:      ::.:|::|||:::.:|||.|   ||.....:..|...|.|||.:|:||.|||..|
Human  1061 AKMQQHVCETIIRIFKRHGAVQLCTPLLLP---RNRQIYEHNEAALFMDHSGMLVMLPFDLRIPF 1122

  Fly  1035 ARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSE 1099
            ||:|..|::..::|||::||:|..::..||||:..:|:|||:..||.|.|..||::...:||..|
Human  1123 ARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQE 1187

  Fly  1100 LPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSR 1164
            .|.|:|:|.:|.:|||.||:|||:.|.:|:.:...::....|.:..:::|.:..:....:...|.
Human  1188 FPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSN 1252

  Fly  1165 TSAQTLMDMLLANFLLTGSRSTVDD--SALKSLMRGKGEAASLARGALRELETVVGLAYSLGVKC 1227
            :..:      |..|:  ..:..:.|  ..:.||::.|...|.|.:..|::||.||||...||:|.
Human  1253 SLCR------LYKFI--EQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKL 1309

  Fly  1228 PIHIWAGLPISFDRASNGGIVWQMTADLKPNRSGHPSVLAIGERYDSMLHEFQKQAQKFNPAMPA 1292
            .:.|..||.....:  :.||::|..|.:|..:...|.:||.|.|||.::.:| :..|...|
Human  1310 QVLINLGLVYKVQQ--HNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQF-RGPQALGP---- 1367

  Fly  1293 RGVLSGAGLTFSLDKLVAAV-GVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIV 1356
              |.:..|::.::||:.||| .:|.:....:.|:.:...|.....:.:. :.:.||:.||...|:
Human  1368 --VPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAIN-LTQKLWTAGITAEIM 1429

  Fly  1357 EAASELGDEAQDLARLGAL-HVILVA--ENGSLRVRSFERERFQERHLTRTELVEFIQKMLR--- 1415
            ...|:..:|.|:..|...: :|.||:  |...::|:|||:||..|:.:..||||:.:.:.||
Human  1430 YDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKV 1494

  Fly  1416 SDGLNGTTV-DNFSQLSALGSGDNRSSGGKERERGENGLSTSASNATIKNNYSQLPNLQVTFLTH 1479
            :|..||... ||.:..:..||..|.|           || .....||:      :|.:.|  |..
Human  1495 TDERNGREASDNLAVQNLKGSFSNAS-----------GL-FEIHGATV------VPIVSV--LAP 1539

  Fly  1480 DKPTANYKRRLENQVAQQMSSTLSQFLKKETFV-VLVVELPPAVV--------NAIVGAINPREI 1535
            :|.:|:.:||.|.||..::.::|:...:|.:.: :|.|:||...:        :|...|.|
Human  1540 EKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFN---- 1600

  Fly  1536 RKRETEPEINYVIERFSKYKRYISEINEEVVDYLSDAKTPIVALYSISDSYYRVI 1590
                  ..:..::.|..| :||:..:.:|:.:...:.|..::.|||..|.|||::
Human  1601 ------TTVKQLLSRLPK-QRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRIL 1648

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gcn2	NP_001263142.1	RWD	17..130	CDD:214735	44/112 (39%)
		PK_eIF2AK_GCN2_rpt1	261..476	CDD:270914	52/255 (20%)
		STKc_EIF2AK4_GCN2_rpt2	518..898	CDD:270948	185/428 (43%)
		S_TKc	525..895	CDD:214567	180/412 (44%)
		HisS	974..1414	CDD:223202	138/451 (31%)
		class_II_aaRS-like_core	974..>1137	CDD:294192	65/168 (39%)
EIF2AK4	NP_001013725.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..25		4/11 (36%)
		RWD	18..134	CDD:283440	45/117 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	138..158		9/19 (47%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	227..256		3/28 (11%)
		Pkinase	296..539	CDD:278497	53/259 (20%)
		PK_eIF2AK_GCN2_rpt1	299..538	CDD:270914	52/255 (20%)
		STKc_EIF2AK4_GCN2_rpt2	583..999	CDD:270948	185/422 (44%)
		S_TKc	590..1001	CDD:214567	183/417 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	660..750		17/91 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	765..788		5/22 (23%)
		Histidyl-tRNA synthetase-like	1022..1493		151/507 (30%)
		class_II_aaRS-like_core	1061..1385	CDD:294192	109/343 (32%)
		HisS	1065..1490	CDD:223202	137/445 (31%)
		HGTP_anticodon	1395..1641	CDD:294191	69/277 (25%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165152773
Domainoid	1	1.000	203	1.000	Domainoid score	I2971
eggNOG	1	0.900	-	-		E1_COG0124
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H40891
Inparanoid	1	1.050	735	1.000	Inparanoid score	I603
Isobase	1	0.950	-	0	Normalized mean entropy	S4325
OMA	1	1.010	-	-		QHG53068
OrthoDB	1	1.010	-	-		D433474at33208
OrthoFinder	1	1.000	-	-		FOG0004143
OrthoInspector	1	1.000	-	-		oto90746
orthoMCL	1	0.900	-	-		OOG6_102717
Panther	1	1.100	-	-	LDO	PTHR11042
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2124
SonicParanoid	1	1.000	-	-		X3408
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	17	16.750