DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Gcn2 and gcn2

DIOPT Version :9

Sequence 1:	NP_001263142.1	Gene:	Gcn2 / 43709	FlyBaseID:	FBgn0019990	Length:	1591	Species:	Drosophila melanogaster
Sequence 2:	NP_595991.2	Gene:	gcn2 / 2540500	PomBaseID:	SPBC36B7.09	Length:	1576	Species:	Schizosaccharomyces pombe

Alignment Length:	1724	Identity:	450/1724 - (26%)
Similarity:	756/1724 - (43%)	Gaps:	325/1724 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 KAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLH 69
            |..|..:|.|..|:|.:|:||..|.|:|:.:   :.|..|:..:....|...|...::.....|.
pombe     5 KRLELCKEIQENEIEALKAIFMDDFEELKVR---NAWNVTNGHVYCIHLCSRSANSKSIAKLDLC 66

  Fly    70 VTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPP 134
            :.....||.:.|.|.|:..:.:.:..:..|.::|..::::|.||.||:|||..||.:|.:.....
pombe    67 IELGRSYPYVKPVIKLQNGENVLNSQIRFLLDKLDTKAKDLLGEEMIFELASIVQDYLNDWQSDL 131

  Fly   135 KGSFYD-------QMLQDKQKRDQELQ-DIQRQRESL--QRQTL----IDEVERRK-EMFKTEAK 184
            ...|..       |:..|:::.:.:|| .::|::::|  :.|||    .||::||. |..::.:|
pombe   132 SSQFASLEEERAVQLKHDRERAEVDLQLRLKREKDALFEEEQTLQNKIQDELQRRSYETPQSSSK 196

  Fly   185 RRGEPRRSMSESNPRHPSSSESSENSSPYYR---------------GHIYPSKCLDHRNTETLYF 234
            ::         :|.:..:|.|:.. :|.|:.               ..:.|...:.|.|..||..
pombe   197 KK---------TNSKETTSLETLP-TSIYFDCSISVRDCHDSLVTFNRVLPLYTISHSNLSTLTL 251

  Fly   235 HK-MGRQIQRGCCVGEGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSES 298
            .| ..::|....||                           :.::..::..|       :||:|.
pombe   252 VKPESKEISLQDCV---------------------------FLLRTVRISTP-------YWSTED 282

  Fly   299 KCMGSHRVDEVMASIEKQVSSLSQLQHKNLVS-YECVLCIK-RKEGLLVYLVQDFLLGTSVFS-I 360
               |...:.|    :|.::.||..::|..|.| ||..|..: |..|..:|::|::....::|| :
pombe   283 ---GKREIQE----LEYELESLKVIRHDLLASIYEYQLERETRGYGWRLYVLQEYSPKFTLFSLL 340

  Fly   361 SSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFMDNTGN---VRVSDF--------- 413
            .:.|...::..|..:..:|:.|..||..|:||..|....|.:.::|:   .::.||
pombe   341 QTVLTLDVETVRAFSNNILEGLAELHRLGISHKSLHLDNVVLFHSGHRTFAKLMDFGFTRTLRDM 405

  Fly   414 --------------SLVPN---------------------------LLELLSGAGQSSSCGDLPA 437
                          :::|.                           :|::|.||...:....|..
pombe   406 NASHPFNINSQSITNILPEGLYPPEVSESSFAAASRKTDIWCFGLLVLQMLCGAHVLNKFSSLKL 470

  Fly   438 LGALVESLMPTNSYEMRDFVDKC---NSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNT 499
            :...|..|:| .||:  |.|.:|   :|.:..||.:||....:|.    |...:.|:.|.....:
pombe   471 IMTHVIPLLP-GSYQ--DLVRRCLMRDSRKRPSAIDLLSSHVIRL----GTAVLPPVEQGTFSKS 528

  Fly   500 VQRTGSAMPYQIPTLAL--SQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARS 562
            .:.:.......|..|..  |.||..|:||.|.:||:|.||:|:||:|.:|.|.||:|::.|.:..
pombe   529 ARPSYGGQQDGIIDLLYRKSVSRYETDFEELEFLGRGGFGEVVKVKNRIDGRFYAVKKLVLLSDD 593

  Fly   563 RQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQS-------QQDLSV 620
            :: ..::.|||..||||:||:||||:.:|:|:..:....|:........|||       :|..|:
pombe   594 KE-NSRILREVMTLSRLHHEHVVRYYTAWVETEANDTVTEIISSDSESLSQSLNMAVDFRQSSSL 657

  Fly   621 KPAKSPQLGPTLEED----EDEEDSSSSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEDS 681
            ...|...|....|:|    .||||..:|..:....|  .||.:|....||:        .|:..|
pombe   658 PADKLSSLDIHFEDDYNSSADEEDPEASDISFQYSN--TSDKEGSSDKDSS--------IEEASS 712

  Fly   682 TRGKTNSPKPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRD 746
            .:.:.|.   |...:|||||:|||.:|:..|.|.:  ..|.:|||||:|.|.||:||.:|::|||
pombe   713 VKTQENG---LNATLYIQMEYCEKLSLQDIIRDKI--PVDEMWRLFRQILEALAYIHSRGMMHRD 772

  Fly   747 LKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELT 811
            |||.|||||.:..:|:|||||||.:......:|...   |:.::.||.| || |||.|||||||.
pombe   773 LKPGNIFLDENRNVKLGDFGLATENENYQDNNDKWK---NRQSADEDLT-TG-VGTALYVAPELL 832

  Fly   812 GNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDP--KYEKTV 874
            ...:...|:.|||||:|||||||||. .|.|||||.:.|..:|:.||:.|...   |  :.....
pombe   833 SRRNGVRYDAKVDMYSLGIILFEMCM-TFSTSMERIRIIDTIRSPSISFPSTF---PFSRASHEF 893

  Fly   875 KMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESD 939
            |::..||.|||.:||:::|||.|:.:|| ::....:||.|| .|:||.:..|..|:.....|..|
pombe   894 KVIHCLLQHDPTKRPSSQELLESEAIPP-KVGEEFIQEGLR-LLSNPNTPYYLKLLKVLFGQVPD 956

  Fly   940 EVLEHTYHLGSSR--------AMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPL 996
            ...:.||....|.        :.:.|:|.:..           .|:..||.:||:|||.|.:|.:
pombe   957 RHKDFTYDFNLSEESGVLSKVSDRGWDSLLAC-----------LVRDHVVKVFRRHGAKERESHI 1010

  Fly   997 LSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVF 1061
            |.|.|::.....|   :|.|:..:|.::.||.|....:||:|..|:|...:.|.:..|:||.: .
pombe  1011 LFPKSSQYDKDQA---SVSLLDKNGTLLQLPYDTVLPYARNVARNAVEEEKTYLISDVFREAK-G 1071

  Fly  1062 NFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCN 1126
            ...||...:.||||...:......|||.:....|:.:|:|.|.|.  .|.:||.::|.:||.:..
pombe  1072 GGRPKAIKEISFDITTNSDNLDWYDAETIKALDEVLTEIPSLTES--CILINHADILSSILDYLQ 1134

  Fly  1127 VPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSA 1191
            |.|.: ..:....:..|..|::..|..:.   :..:|...:.||.|:.|.:|     |...::.|
pombe  1135 VSKDK-RRMATHILGQINQRLTLSQVRNQ---LRIESLVPSTTLDDLSLFDF-----RENYEEGA 1190

  Fly  1192 LKSLMR---GKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQMTA 1253
            .|  :|   || |.....|.||..:|.||.|..:|.:...::.......:|: ..:||:::|..
pombe  1191 SK--LRKIFGK-EMPQKMRTALNYMERVVKLLRALKISHQLYFMPLCVYNFE-FYDGGLMFQAI- 1250

  Fly  1254 DLKPNRSGHPSVLAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSLDKLVAAVGVEYAK 1318
                |.:....::..|.|||.::..|..      |.|.........|:.|:|:|||.:: :.|.:
pombe  1251 ----NLAEKSELICAGGRYDKLVRFFDP------PLMRTARKKHVVGICFALEKLVFSM-LRYIR 1304

  Fly  1319 ----------------------DCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIV-EAAS 1360
                                  ..|.:||.:...| :..:.:...:::.||::.|:..|| ..||
pombe  1305 FHNSKQSSKHSPSPTLKSVGPWAPRRVDVLVTSIG-KDSILEKCSLLQELWALNIQADIVLRGAS 1368

  Fly  1361 ELGDEAQDLARLGALHVILVAENGSLRVRSFERERFQERHLTRTELVEFIQKMLRSDGLNGTTVD 1425
            .|.:........|...|::|.:..:....|     .:.|::.:.|..|     :|.|.:.
pombe  1369 SLEEIVTHYRSEGINWVLVVRQKNTQMEHS-----VKARNILKNEDDE-----IRFDEVG----- 1418

  Fly  1426 NFSQLSALGSGDNRSSGGKERERGENGLST-----SASNATIKNNYSQLPNLQVTFLT----HD- 1480
                :..||..:       ||:|.|:.|.:     ||.....|...:...||.|..::    :|
pombe  1419 ----MWLLGEIN-------ERKRNESMLQSKRILDSAQQDVAKFVDTSQSNLDVQLISLKDVNDR 1472

  Fly  1481 KPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREIRKRETEPEIN 1545
            |....:|:...|:|...:.|.:.:  ..|..:.|.|:.....:         .::|...|..|
pombe  1473 KYKWKHKQNAMNKVYDLVQSAIRE--SSEDAIALAVDCDSEAM---------EKLRSTTTLDE-- 1524

  Fly  1546 YVIERFSKYKRYISEINEEVVDYLSDAKTPIVAL 1579
                  ..:||.|........:|:...:..:|.|
pombe  1525 ------ESWKRLIESCPASQREYMQRLQKKLVTL 1552

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gcn2	NP_001263142.1	RWD	17..130	CDD:214735	31/112 (28%)
		PK_eIF2AK_GCN2_rpt1	261..476	CDD:270914	54/273 (20%)
		STKc_EIF2AK4_GCN2_rpt2	518..898	CDD:270948	159/392 (41%)
		S_TKc	525..895	CDD:214567	154/382 (40%)
		HisS	974..1414	CDD:223202	113/465 (24%)
		class_II_aaRS-like_core	974..>1137	CDD:294192	51/162 (31%)
gcn2	NP_595991.2	RWD	10..124	CDD:283440	32/116 (28%)
		PK_eIF2AK_GCN2_rpt1	241..508	CDD:270914	62/310 (20%)
		STKc_EIF2AK4_GCN2_rpt2	549..917	CDD:270948	159/392 (41%)
		S_TKc	556..915	CDD:214567	155/383 (40%)
		HisS	990..1417	CDD:223202	115/468 (25%)
		class_II_aaRS-like_core	990..1295	CDD:294192	90/334 (27%)
		HGTP_anticodon2	1326..1576	CDD:289514	52/273 (19%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	165	1.000	Domainoid score	I937
eggNOG	1	0.900	-	-		E1_COG0124
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H40891
Inparanoid	1	1.050	384	1.000	Inparanoid score	I414
OMA	1	1.010	-	-		QHG53068
OrthoFinder	1	1.000	-	-		FOG0004143
OrthoInspector	1	1.000	-	-		oto101657
orthoMCL	1	0.900	-	-		OOG6_102717
Panther	1	1.100	-	-	LDO	PTHR11042
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2124
SonicParanoid	1	1.000	-	-		X3408
TreeFam	1	0.960	-	-
	14	13.860