Protein Alignment Gcn2 and gcn-2

DIOPT Version :10

• Sequence 1: NP_001263142.1
• Gene: Gcn2 / 43709
• FlyBaseID: FBgn0019990
• Length: 1591
• Species: Drosophila melanogaster
• Sequence 2: NP_001367229.1
• Gene: gcn-2 / 174953
• WormBaseID: WBGene00013591
• Length: 1699
• Species: Caenorhabditis elegans
• Alignment Length: 1869
• Identity: 441/1869 - (23%)
• Similarity: 749/1869 - (40%)
• Gaps: 459/1869 - (24%)

  Fly     3 DEKAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSS--NG---LET 62
            ||:.||: :..|.:|...:.:::...:..::  |:..:|.||:..|.|..| ||.  ||   .::
 Worm    10 DERVKEN-QHLQEEEKLALDAVYLNQITYIK--AHWHVWVPTNCHILLKAL-DSCFLNGDPLGKS 70

  Fly    63 YVCTKLHVTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFL 127
            .:...|||.|...||:..|.:.|.:.:|:|.:.::.|...|:..:....|.|:|.|||..|:.||
 Worm    71 KLSVILHVKCSEDYPQRKPAVDLLDPQGLSKEDVQNLLTILRQMADTWEGCVVIAELAHRVREFL 135

  Fly   128 LEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQRESLQRQTLIDEVERRK--EMFKTEAKRRGE-- 188
            .:|...|.|||:|.||.:|.:.:.|.|..:...|..:.:.|.:|:.:|.  ||.||....|.|
 Worm   136 TDHTPRPAGSFHDDMLANKVRTEAEKQRKRLDTEQKELELLEEEMRQRNAIEMEKTLNGTRQENE 200

  Fly   189 -----PRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVG 248
                 .||.:..||                     .|       ||:
 Worm   201 TRIIGGRRIVVLSN---------------------MP-------NTQ------------------ 219

  Fly   249 EGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASI 313
                                 |.|:||..::|....|..|..|      .......::|.|...|
 Worm   220 ---------------------LLISEWTFRFSSNRNPAEGKRK------DFAPFLQKLDAVYNEI 257

  Fly   314 EK--QVSSLSQLQHKNLVSYECV-----------LCIKRKEGLLVYLVQDFLLGTSVFSISSSLG 365
            :|  ::..|.|    |||.|..|           :.|:......::..::.:..|....:..|
 Worm   258 QKLCEIKGLDQ----NLVEYAFVHLQKISVSPDQILIQLNVAQKIFSSEENMQDTYELIVQKS-- 316

  Fly   366 WCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFMDN-TGN--VRVSDFSLVPNLLELLSGAG 427
               :..|::|...:..|.:||...::|.||...:|:..| ||:  .|.|||..:..||:|:...|
 Worm   317 ---NLLRLLAAQAICGLRYLHEASMTHKHLTLGSVWTRNSTGDCVFRFSDFGSMGPLLDLVKMFG 378

  Fly   428 QSSSCG------------------DLPALGALVESLM-------------------PTNSYEMRD 455
            ...| |                  ||..||.|::.|:                   .|.:..:.:
 Worm   379 DICS-GKYVARDEDKEKEYDRRRKDLFQLGTLLDGLILATRGSTYSRVPTPVEGNQNTGTNLLGN 442

  Fly   456 FVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSR 520
            |:.||...:.:  .:|:|.|||:...           |.:..|.....|.||.        ...|
 Worm   443 FIAKCQEAKNI--DQLVEDPFLKEEC-----------QSESENIFTPFGGAMS--------PDGR 486

  Fly   521 LRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVV 585
            :..:..::..||:|.||||:.|||.:|:.:||||||||.|:|.:|.:|:.:|.:..::|||.|:|
 Worm   487 MLADNVIIRVLGRGGFGDVVLVRNKMDSTDYAIKRIPLNAKSDKLNRKIAKEAKFFAKLNHPNMV 551

  Fly   586 RYFNSW-------IESVDDADAA-------------EMDKL--------------LGG------- 609
            ||:.:|       :|...|.|::             :..||              |||
 Worm   552 RYYYAWAEDLIPIVEETSDDDSSLGAVPIPGKEKIGKKGKLKTGKSLEDKENKANLGGGDSLMPM 616

  Fly   610 ----------------EWS--------------QSQQDLSVKPAKSPQLGPTLEEDEDEEDSSSS 644
                            |||              ..|...|........|.....:|||::|||..
 Worm   617 NLRGLVKDHSIGVDAKEWSTPFGKPEGPKCASRMRQSKRSTPSGGLKHLSECSSDDEDDDDSSEI 681

  Fly   645 MWNGYIPNMEDSDSDGIEFVD--------------SNGKVAVYD----DE------EQEDSTRGK 685
            .|:.....:||.:||..:..|              |.|..:|::    ||      |.||....:
 Worm   682 DWDAESEEVEDEESDDSDEEDEDDGERLVQLNTETSTGADSVFERSTADEDVVFTAESEDLNAKR 746

  Fly   686 TNS---------------------PKPLMQVMYIQMEFCEKCTLRTAIDDN-LFNDTDRLWRLFR 728
            ..|                     |....:::.||||:|::.|||..||:| .||....:||:|.
 Worm   747 RESIELMEINTTTTSKSKLAIDVVPVRKPRILCIQMEYCDRATLRQYIDENHCFNAPTEVWRIFS 811

  Fly   729 EIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAH------------DAA 781
            |:..||.::|...:||||:||:||||.|.:.:|||||||||...::.:..            :|.
 Worm   812 EVLCGLKYMHDMAMIHRDIKPLNIFLTSQNGVKIGDFGLATLEAMSSKGKIVGGAAEKSTSIEAM 876

  Fly   782 PAPVNQITSAEDGTGTGKVGTTLYVAPELTGN--ASKSVYNQKVDMYTLGIILFEMCQPPFDTSM 844
            .:|....:...|...|..:||.||:||||..:  ..|:.|..|:|:|:.|::||||...|...||
 Worm   877 LSPNGVKSKGSDVHQTRDIGTQLYMAPELFVDELVHKAPYTSKIDIYSAGVVLFEMFYRPLPPSM 941

  Fly   845 ERAQTIMALRNVSINIPD---AMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLE 906
            :|..|:..||: .|.||.   |.|..|......:.::.:|..:|.:||||::||..:.:|....|
 Worm   942 DRVSTLNNLRD-DIKIPSDFGAGLAAPMAGLARRTVEKMLQRNPDERPTADDLLNDEDLPMHTKE 1005

  Fly   907 ANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNP 971
            ....:.:....:.....:....|:.:..::|....|.:.|.:........:|:..::
 Worm  1006 DATFRNLCEKVIKKRDGRMNAWLLDKQFKEEVPTSLNYCYDVDICLERAKYNNREVL-------- 1062

  Fly   972 VIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFAR 1036
             :|.::|:...:.:.|...::.:..|.|:|...:.|:.....|.::..||..|.||.|||..|.|
 Worm  1063 -VETLRAEFCKILKIHSFEKLHTHTLMPVSTALAAASVRTKPVEVLDRSGVPVALPMDLRQNFVR 1126

  Fly  1037 HVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFE-ITSEL 1100
            ....|||..::|:...|||.:... |.||.:.::|..|.|.|...|..::||||.:|.| :...|
 Worm  1127 FCVRNSVQRMKRFNFGRVYSQTSA-NGHPHERWECCVDCIGPQCSSPSLEAELLLVACEMMIGSL 1190

  Fly  1101 PRLREKNLAIRMNHTNLLRAILIFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITG------- 1158
            |.::   ..:::.|..|                         ||::|...:....:..
 Worm  1191 PGMK---FTLKIGHAQL-------------------------IEAQIRHLKLSDDVRAELLDALH 1227

  Fly  1159 -IMEKSRT-SAQTLMDML-------LANFLLTGSRSTVDDS--ALKSLM-----RGKGEAAS-LA 1206
             |....|. |.:..||||       .|| ::|.....|:|:  |.|..:     :.|.:||. |.
 Worm  1228 LISVSDRPHSHKEKMDMLTPKIGAKAAN-IITKLLIPVEDNFGAFKEKVACFRKKLKVDAARVLV 1291

  Fly  1207 RGALRELETVVGLAYSLGVKCPIHIWAGLPISFD-------RASNGGIVWQMTADLKP-----NR 1259
            ..|:|:||.:|| .:..   |.......:.|.:|       |....|:::|:..: ||     |:
 Worm  1292 DKAIRDLEEIVG-TFKF---CRTEAIEQISIVYDSQTCYRPRTFGDGLLFQIQVE-KPSTIANNK 1351

  Fly  1260 SGHPSVLAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSL-----DKLVAAVGVEYA-- 1317
            .|....:..|.||||.|.. ::..:.|...:|.  .:||.|:...:     |.:..:..:...
 Worm  1352 RGRRQNVLAGGRYDSALLR-ERHPRDFVYEIPL--CISGFGVAMDVVSQIRDSINKSANIPKTPQ 1413

  Fly  1318 KDCRAIDVGICVCGTRPP-----LKDVTYIMRLLWSVGIRCGIVE-AASELGDEAQDLARLGALH 1376
            ..|:     :.:|....|     :.....:.:.|||:||...:.. ...:|....:...|....|
 Worm  1414 NHCK-----VLICSMVQPDGSNLITQKFELAKKLWSMGIEADVFHIPVDDLESLTEHRNRASITH 1473

  Fly  1377 VILVAENGSLRVRSFERERFQERHLTRTELVEF---IQKMLRS-DGLNGTTVDNFSQLSALGSG- 1436
            ::.|....:..:...|         |.:|.::.   |..:.|. ..|:|.::    .::..|.|
 Worm  1474 ILAVYNTLNEVICKTE---------TSSETMDVDSAISSVWRGVQALDGQSI----HMTPCGGGP 1525

  Fly  1437 -DNRSSGGKERERGE--------------NGLSTSASNATIKNNYSQLPNLQVTFLTH-DK--PT 1483
             .:.|:.|:.....:              :.:||:.:..:|:...:.:.||.|..:|. |:  ..
 Worm  1526 ISSISTPGEAHHHDDHHPGTPVIASKCFRSSVSTTVATTSIRPISATVANLNVILVTSADRFHKV 1590

  Fly  1484 ANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREIRKRETEPEINYVI 1548
            ...|:|:|:||...::..::.|..|....|||.::|..|:..||.     |:.|..:|.||:.:.
 Worm  1591 MKEKKRVESQVRNHLTEFVAHFTSKTRIEVLVCDIPADVIKKIVS-----ELTKTSSEAEIDKLF 1650

  Fly  1549 ER-FSKY-KRYISEINEE---VVDYLSDAKTPIVAL-YSISDSYYRVII 1591
            :: ..|: |..:|.:..:   .::.:|.....:..| |..||::||.::
 Worm  1651 DQLIQKHGKVDLSPLRRQFHITLNGISTGSAQVAILFYRQSDNFYRYLV 1699

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Gcn2	NP_001263142.1	RWD_GCN2	11..131	CDD:467659	34/124 (27%)
		PK_eIF2AK_GCN2_rpt1	261..476	CDD:270914	56/267 (21%)
		STKc_EIF2AK4_GCN2_rpt2	518..898	CDD:270948	152/513 (30%)
		class_II_aaRS-like_core	974..>1137	CDD:444800	43/163 (26%)
		PRK12420	1029..1392	CDD:237097	91/412 (22%)
gcn-2	NP_001367229.1	RWD_GCN2	17..138	CDD:467659	34/123 (28%)
		Protein Kinases, catalytic domain	217..>373	CDD:473864	42/209 (20%)
		Protein Kinases, catalytic domain	486..>560	CDD:473864	33/73 (45%)
		Protein Kinases, catalytic domain	<776..997	CDD:473864	83/221 (38%)
		class_II_aaRS-like_core	1106..>1227	CDD:444800	38/149 (26%)