Protein Alignment Gcn2 and eif2ak4

DIOPT Version :9

• Sequence 1: NP_001263142.1
• Gene: Gcn2 / 43709
• FlyBaseID: FBgn0019990
• Length: 1591
• Species: Drosophila melanogaster
• Sequence 2: XP_012823581.1
• Gene: eif2ak4 / 100216307
• XenbaseID: XB-GENE-967321
• Length: 1674
• Species: Xenopus tropicalis
• Alignment Length: 1769
• Identity: 534/1769 - (30%)
• Similarity: 854/1769 - (48%)
• Gaps: 312/1769 - (17%)

  Fly     8 ESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWK---PTDIRIQLTPLRDSSNGLETYVCTKLH 69
            |::..||..||||::||:..|..|||..   |.||   |.::.|.|.|     .|.|.:|...|.
 Frog    31 ETYSHRQENELEVLESIYSGDFADLRKN---SAWKVQQPPEVIIALRP-----QGNEVHVKVDLW 87

  Fly    70 VTCPSKYPKLPPKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPP 134
            |.||..||.:||:|.|:..:|:|::.:.:|.:.|:..:::|.||||||:||..||.||.|:|.||
 Frog    88 VKCPQNYPDVPPEIKLKNIQGLSNENVNSLTSSLEDLAKQLCGEVMIYQLADHVQCFLTEYNHPP 152

  Fly   135 KGSFYDQMLQDKQKRDQEL--------QDIQRQRESLQRQTLIDEVERRKEMFKTEAKRRGEP-- 189
            ..||:::||::.||:.::|        |:|:.:.|..:|: :::|:::|:|. |.|.|||.|.
 Frog   153 PKSFHEEMLKNLQKQQEKLALEEERRMQEIKMKEEQTRRE-ILNEIQKREEE-KKEEKRRKEVAK 215

  Fly   190 -----------RRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQ--- 240
                       :.::::.|....:::.:|:.|.|            ||..|.....|..||.
 Frog   216 QERIENANLMCQENVNKINNTGKTTTAASDGSLP------------DHGGTNRNRPHSAGRSKRD 268

  Fly   241 ----------------------------IQRGCCVGEGHSQRGCIAYTGIDMHCGQLLYITEWNI 277
                                        :.||.|:|: ....|...|.|::...|..:.|.||.:
 Frog   269 RQYSVCLTDDSTPFEVLTFDIGCSGQIVVHRGKCLGK-DELLGKSVYNGLETASGHFVVIYEWVL 332

  Fly   278 KYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEK----------QVSSLSQLQHKNLVSYE 332
            ::                  .|.||....::....|||          :.|||.:|.|.|:|.|.
 Frog   333 QW------------------QKKMGKFLTNQEKEKIEKCKKQIQGTETEFSSLIKLSHPNIVHYL 379

  Fly   333 CVLCIKRKEGLLVYLVQDFLLGTSVFS-ISSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLL 396
            .:...::::.:.|.::.:.:.||.:.| :...:...:|..|.....:|.||.:||:..|.|..:.
 Frog   380 AMRIKEKEDSISVGIIAEHVNGTKLSSYLEQGVPVPLDQLRHYTAQLLSALDYLHSNSVVHKVMC 444

  Fly   397 DTTVFMDNTGNVRVSDFSLVPNLLELLSG---------------AGQSSSCGDLPALGALVESLM 446
            .::|.:...|.|:::|:||...|.::...               ..:|...||:..||.|:.||.
 Frog   445 ASSVLVTADGKVKLTDYSLSKRLADICKEDVFEQTKVRFSEDALPSKSGKKGDIWKLGLLLLSLS 509

  Fly   447 PTN---SY----------EMRDFVDKC---NSDRTLSASELLEHPFLRFYVDNGQQQVMPLPQQQ 495
            ..|   .|          :.:||:.||   ......:..:||.|.||.           | |.::
 Frog   510 QGNIAKEYPVKVPSDLPADFQDFLQKCVCLEDKERWTTQQLLSHSFLN-----------P-PPEK 562

  Fly   496 HPNTVQRT----GSAMPYQIPTLALSQ-----------SRLRTEFEVLMYLGKGAFGDVLKVRNI 545
            .|.|.:.:    |......||..::|.           ||...|||.|..|||||||.|:||:|.
 Frog   563 SPVTEECSDDYPGDCTETVIPRSSVSNSTFHIESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNK 627

  Fly   546 LDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGE 610
            ||...||:||:.:...|:| ::::..||.|||||||||:|||:|:|||..:....:...:
 Frog   628 LDGCYYAVKRVQINPASKQ-FRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCE----- 686

  Fly   611 WSQSQQDLSVKP-----------------AKSPQLGPTLE-EDEDEEDSSSSMWNGYIPNMEDSD 657
             :..::.::|||                 |.:|.|..::| ....|..||:...||...:.:|.|
 Frog   687 -AHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDD 750

  Fly   658 SDGIEFV---------DSNGKVAV-------------YDDEEQEDSTRGKTNSPKPLMQVMYIQM 700
            .|..|.|         ||:.::..             ||....||...|........:..:||||
 Frog   751 DDDEEGVFSPSFLPGTDSDSEIIFENEDDISPDQDEEYDKNNVEDRKAGANEIVTQAVHYLYIQM 815

  Fly   701 EFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDF 765
            |:|||.|||..||..|:.|..|||||||||.:||.:||:||:||||||||||||||.|.:|||||
 Frog   816 EYCEKSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFLDSEDHVKIGDF 880

  Fly   766 GLAT------------------TSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPELTG 812
            ||||                  :..:.:...|.|..|...    ..|..||.|||.|||:||:.|
 Frog   881 GLATDHPASYTESAQGEEDQAHSDLVKVDPEDRASTPGRN----TQGKLTGMVGTALYVSPEVQG 941

  Fly   813 NASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKML 877
            | :|:.||||||:|:||||||||...|.|||.||...:..||...|..| :..::|:.||..|::
 Frog   942 N-TKASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFP-SDFENPENEKQKKVI 1004

  Fly   878 QWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVL 942
            .||||||||.||||.|||..||:||.|||.:||.|:|.|.|||...|||:.::::...|.....:
 Frog  1005 TWLLNHDPASRPTAMELLKCDLLPPPQLEESELHEVLHHTLANIDGKAYRTMMSQIFAQRISPAM 1069

  Fly   943 EHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTA 1007
            ::||.....:.:.|..||.:...:..          .|..:|::|||:::.:|||.|   |....
 Frog  1070 DYTYDSDILKGIFSVRSARLHHHVCE----------AVCRIFKRHGAVKLHAPLLMP---RGKAG 1121

  Fly  1008 NANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCS 1072
            ..|:.:...|.|||.:|.||.:||..|||.|..|::..::||.::||:|..::...|||:..:|:
 Frog  1122 FDNSESACFMDHSGMLVTLPYNLRISFARFVARNNITSLKRYSIERVFRPRKLNRCHPKELTECA 1186

  Fly  1073 FDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQ----YG 1133
            ||||......:|..||.:...:||..|.|.|:|:|.:|.:|||:||:|||:.|.||:.:    |.
 Frog  1187 FDIITSAANCYLPIAETIYTIYEIIQEFPVLQERNYSIYVNHTSLLKAILLHCGVPEDKLTQVYN 1251

  Fly  1134 ALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDD--SALKSLM 1196
            .|::..::.:..|....:|.:     :..|..|.|     |...:.....:..:.|  :.:..|.
 Frog  1252 ILYDAMIEKLTKREVEAKFCN-----LSLSENSFQ-----LSRLYRFIEQKGDLPDLEAMMVPLT 1306

  Fly  1197 RGKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQMTADLKPNRSG 1261
            :.|.....||:..|::||.|:||...||:|..|.:..||.....:.|  |||:|..|.:|..:..
 Frog  1307 KQKTGVGVLAKQGLKDLEGVIGLVKRLGIKLQIVVNFGLVYKMQQHS--GIVFQFVAFIKRRQRT 1369

  Fly  1262 HPSVLAIGERYDSMLHEFQKQAQKFNPAMPARGVLSGAGLTFSLDKLVAAVGV--EYAKDCRAID 1324
            .|.:||.|.|||.::.:|:      .|...|.|:....|::.::||:.||:..  |:...|   |
 Frog  1370 VPEILAAGGRYDHLISQFR------GPQTGAMGLPCAVGVSIAIDKISAALSTAEEHISSC---D 1425

  Fly  1325 VGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASELGDEAQDLARLGALH---VILVAENGSL 1386
            : :.|...:..|.....|.:.||:.||...|:...||..||.||..:...:.   :|...|...:
 Frog  1426 L-LVVSAGQMSLNRAIGITQKLWAAGITAEIMYEWSESQDELQDYCKCSGISYAAIISDKEGNHI 1489

  Fly  1387 RVRSFERERFQERHLTRTELVEFIQKMLR----SDGLNGTTVDNFSQLSALGSGDNRSSGGKERE 1447
            ::::||:||..|:.:..::||:.:.:.||    .:..|..|.|:.|..:..||.:::|
 Frog  1490 KLKTFEKERQSEKRILESDLVDLLVQKLRIRVLEERNNRETSDSLSVQNVKGSFNSQS------- 1547

  Fly  1448 RGENGLSTSASNATIKNNYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFV 1512
                  .....:|.|      :|::.|  :..:|.:|:.:||.|.||..::.:.::.|.::...:
 Frog  1548 ------QFEPHSAII------VPSVSV--IAPEKLSASARRRFETQVLTKLQTLITNFQQRNGEI 1598

  Fly  1513 -VLVVELPPAVVNAIVGAINPREIRKRETEPEINYVIERFSKYKRYISEINEEVVDYLSDAKTPI 1576
             :|.|:||...:..:: |:...:  ::.....:..::.|..| :||:..:.:|:.....:.:.|:
 Frog  1599 EILAVDLPKETIQNLL-ALECAD--EQVFNASVKMLMSRLPK-QRYLKSVFDEIYTIKIEKRVPV 1659

  Fly  1577 VALYSISDSYYRVI 1590
            :.|||..|.||:::
 Frog  1660 LVLYSYKDDYYKIL 1673

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Gcn2	NP_001263142.1	RWD	17..130	CDD:214735	47/115 (41%)
		PK_eIF2AK_GCN2_rpt1	261..476	CDD:270914	56/256 (22%)
		STKc_EIF2AK4_GCN2_rpt2	518..898	CDD:270948	182/448 (41%)
		S_TKc	525..895	CDD:214567	177/427 (41%)
		HisS	974..1414	CDD:223202	134/450 (30%)
		class_II_aaRS-like_core	974..>1137	CDD:294192	62/166 (37%)
eif2ak4	XP_012823581.1	RWD	33..142	CDD:283440	45/116 (39%)
		Pkinase	305..556	CDD:278497	58/269 (22%)
		PK_eIF2AK_GCN2_rpt1	316..555	CDD:270914	56/256 (22%)
		STKc_EIF2AK4_GCN2_rpt2	600..1025	CDD:270948	182/437 (42%)
		S_TKc	607..1023	CDD:214567	178/428 (42%)
		HisS	1087..1517	CDD:223202	135/464 (29%)
		class_II_aaRS-like_core	1087..1414	CDD:294192	108/357 (30%)
		HGTP_anticodon	1422..>1613	CDD:294191	50/215 (23%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	212	1.000	Domainoid score	I2727
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H40891
Inparanoid	1	1.050	707	1.000	Inparanoid score	I645
OMA	1	1.010	-	-		QHG53068
OrthoDB	1	1.010	-	-		D433474at33208
OrthoFinder	1	1.000	-	-		FOG0004143
OrthoInspector	1	1.000	-	-		oto104541
Panther	1	1.100	-	-	LDO	PTHR11042
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3408
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	11.080