Protein Alignment PH4alphaEFB and PH4alphaNE2

DIOPT Version :9

• Sequence 1: NP_733371.1
• Gene: PH4alphaEFB / 43620
• FlyBaseID: FBgn0039776
• Length: 550
• Species: Drosophila melanogaster
• Sequence 2: NP_733378.1
• Gene: PH4alphaNE2 / 43628
• FlyBaseID: FBgn0039783
• Length: 542
• Species: Drosophila melanogaster
• Alignment Length: 554
• Identity: 170/554 - (30%)
• Similarity: 271/554 - (48%)
• Gaps: 52/554 - (9%)

  Fly     5 WRLMLLLGILLLV-------GGPANGEVYTALAEMEELLETESVLITNLEGYIRVQEDKLNFLK- 61
            ||| :|||:|.|.       ...|......::..|:::|..|..|::|:|........|...:|
  Fly     4 WRL-VLLGMLYLSLSFGQLRENNAQQRFARSVVNMDDMLNLEDDLVSNVEKLAEALARKAKTIKW 67

  Fly    62 --NKMDEYQREHSDASHDITAYVSNPINAYLLTKRLTTDWRQVENLMEHDVGTDFLQNITQYRSL 124
              .||.:.::|:. :|.:|.|   |||:.:.|.:.:.::|......:|..||.:.|..:.....|
  Fly    68 GVFKMMKRRQEYK-SSMEIFA---NPIDTFSLIRHMQSNWLMWLLYLETPVGQEELFFVDSRMPL 128

  Fly   125 LKFPSDEDLNGAAVALLRLQDTYQLDTSSVARGKLNGIQYSTEMSSDDCFELGRQSYVNHDYYHT 189
            |  |...|...||..:.::|.|||:.:|.:|:|.|:|:||::.:...||..:|.....|..:|..
  Fly   129 L--PKYFDFIDAAEGIRKMQATYQMFSSDIAKGLLDGVQYNSSLKPIDCLAIGLHLMNNSRWYAA 191

  Fly   190 VLWMNEAMARMLEEPTNHTQSFTK----ADILEYLAFSTYKEGNIESALTMTNELLQLLPHHERA 250
            ..|::.::....::.:.......:    ||:...|.....|:.|.|.||......|:|.||
  Fly   192 EQWISASIEAYDQKSSQTDMELLRGPKLADLCRILGQVQMKQRNHEGALQAYQVALKLSPH---- 252

  Fly   251 NGNKRFYEKEIAQQLQLRKMKGDDGTDEMPKSDLPVAKSDPAIFDMT---ERRAYEMLCRGELKP 312
                   :.||.::.::           :.|.||.::..:|...|..   ||......|.|..:
  Fly   253 -------DPEIYEEYRI-----------LEKRDLTLSDIEPIEQDKDNSHERLVLPPCCSGRCQ- 298

  Fly   313 SPSDLRSLRCRYVTNRVPFLRLGPLKLEEVHADPYIVIYHDAMYDSEIDLIKRMARPRFRRATVQ 377
            .|.:|.:|.|.|.....|||:|.|:|.|.:..||::|:.||.:...|..||:..::.....:...
  Fly   299 VPRNLSNLYCVYNHVTSPFLQLAPIKTEILSIDPFVVLLHDMISQKESTLIRTSSKEHMLPSATT 363

  Fly   378 NSVTGALETA--NYRISKSAWLKTQEDRVIETVVQRTADMTGLDMDSAEELQVVNYGIGGHYEPH 440
            :......||.  .||.|||.|..:..:...:.:.:|..|.|||||:|.|..||:|||:||.:|.|
  Fly   364 DPDASDDETQVDTYRTSKSVWYSSDFNDTTKKITERLGDATGLDMNSTEFYQVINYGLGGFFETH 428

  Fly   441 FDFARKEEQRAFEGLNLGNRIATVLFYMSDVEQGGATVFTSLHTALFPKKGTAAFWMNLHRDGQG 505
            .|....|:.| |.|.:  :||||.|||:::|.|||.|.|..|:..:||:.|:|.||.||...|..
  Fly   429 LDMLLSEKNR-FNGTS--DRIATTLFYLNEVRQGGGTYFPRLNLTVFPQPGSALFWYNLDTKGND 490

  Fly   506 DVRTRHAACPVLTGTKWVSNKWIHERGQEFRRPC 539
            .:.:.|..|||:.|:|||.:|||::.||||.|||
  Fly   491 HMGSLHTGCPVIVGSKWVMSKWINDMGQEFTRPC 524

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PH4alphaEFB	NP_733371.1	P4Ha_N	28..159	CDD:285528	36/133 (27%)
		P4Hc	356..529	CDD:214780	67/174 (39%)
PH4alphaNE2	NP_733378.1	P4Ha_N	33..161	CDD:285528	36/133 (27%)
		TPR repeat	175..200	CDD:276809	5/24 (21%)
		TPR_16	176..254	CDD:290168	16/88 (18%)
		TPR repeat	219..249	CDD:276809	9/29 (31%)
		TPR_2	220..253	CDD:285020	12/43 (28%)
		P4Hc	342..513	CDD:214780	66/173 (38%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45461815
Domainoid	1	1.000	91	0.514	Domainoid score	I2609
eggNOG	1	0.900	-	-		E1_KOG1591
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	79	1.000	Inparanoid score	I2378
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D192165at33208
OrthoFinder	1	1.000	-	-		FOG0000199
OrthoInspector	1	1.000	-	-		mtm1209
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR10869
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X129
	10	9.900