DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sima and hif1a

DIOPT Version :9

Sequence 1:	NP_001287599.1	Gene:	sima / 43580	FlyBaseID:	FBgn0266411	Length:	1593	Species:	Drosophila melanogaster
Sequence 2:	XP_031746581.1	Gene:	hif1a / 496583	XenbaseID:	XB-GENE-946622	Length:	796	Species:	Xenopus tropicalis

Alignment Length:	876	Identity:	270/876 - (30%)
Similarity:	402/876 - (45%)	Gaps:	224/876 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly   154 MNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVP 218
            :::|:||||||||||||||||:|:|.|||..|||..:..:.|||||:||:||::|:||.:|
Frog    13 ISSERRKEKSRDAARCRRSKESEVFYELSHELPLPHNVSSHLDKASIMRLTISYLRIRRLL---- 73

  Fly   219 SLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSE 283
               |..|   .|.||..|:|             ||.     ...:.::||:|||:.|||:.|:||
Frog    74 ---DAGD---LDGETELDKQ-------------LNC-----FYLKALEGFVLVLTEEGDMIYLSE 114

  Fly   284 NVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRL 348
            ||.:.:|:|:.:..|..:::::|.|||.|::|.|:.:...|:|.|::..:        |..|:|:
Frog   115 NVNKCMGLTQFELTGHSVFDFTHPCDHEELREMLTFRNGPAKKGKEQITE--------RSFFLRM 171

  Fly   349 KCTLTSRGRSINIKSASYKVIHITGHLVV------NAKGER-----LLMAIGRPIPHPSNIEIPL 402
            |||||||||::|||||::||:|.|||:.|      |..|.:     .::.|..|||||||||.||
Frog   172 KCTLTSRGRTVNIKSATWKVLHCTGHMRVYDANNHNHCGYKKPPMTCMVIICEPIPHPSNIEFPL 236

  Fly   403 GTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGET 467
            .:.|||::|||||:|:|.|:::.:|:||.|.:||..|::...|..||:.|.....::.:|||..|
Frog   237 DSKTFLSRHSLDMKFSYCDERVTELVGYEPDELLGRSVYEYYHALDSDHLTKAHHNMFTKGQVTT 301

  Fly   468 SRYRFLGKYGGYCWILSQATIVYD--KLKPQSVVCVNYVISNLENKHEIYSLAQQTAA------- 523
            .:||.|.|.|||.|:.:|||::|:  ..:||.:||||||:|.:..|..|.||.|....
Frog   302 GQYRMLAKKGGYVWVETQATVIYNSKNSQPQCIVCVNYVLSEVVEKDLILSLGQTACVLSPVESQ 366

  Fly   524 --------SEQKEQHHQAAETEK---EPEKA------ADPEIIA------QETKETVNTPIHTSE 565
                    :|..|.::.....||   |||..      |..|||:      ...|...:.|::...
Frog   367 EMKMPEIFTELNEDNNAECLFEKLKQEPESLTVLAPDAGDEIISLDFSSGDSDKPYEDVPLYNDV 431

  Fly   566 LQAKPLQLESEKAEKTIEETKTIATIPPVTATSTADQIKQLPESNPYKQILQAELLIKRENHSPG 630
            :    |...|:|.|.|           |||....::..|.| .|| ....|..|::||.|::
Frog   432 M----LHSTSKKLEST-----------PVTPLPASEMPKPL-RSN-VDPALNREVVIKMESN--- 476

  Fly   631 PR----TITAQLLSGSSSGLRPEEKRPKSVTASVLRPSPAPPLTPPP-----TAVLCKKTPLGVE 686
            |.    |.|...||      :|:.  |..:::|   .|...|.|||.     .:.:..:..|.:.
Frog   477 PEQLELTFTIPQLS------KPDS--PSDISSS---QSSTEPSTPPEYCFDVDSEIASEFKLDLV 530

  Fly   687 PNLPPTTTATAAIISSSNQQLQIAQQTQLQNPQQPAQDMSKGFCSLFADDGRGLTMLKEEPDDLS 751
            ..|....|...|..|:....|.:    ::..|..|            .||...|....:
Frog   531 EKLFAIDTEAKAPFSTQETDLDL----EMLAPYIP------------MDDDFQLRTFDQ------ 573

  Fly   752 HHLASTNCIQLDEMTPFSDMLVGLMGT----CLL-------PED-INSLDSTTCSTTASGQHYQS 804
              |:|..|   |...|   ..:|.|.|    .||       ||| ||.|.:.          .||
Frog   574 --LSSLEC---DSSNP---QTLGSMATLFQQSLLPSSSDFKPEDAINDLKTI----------IQS 620

  Fly   805 PSSSSTSAPSNTSSSNNSYANSPLSPL-------------TPNSTATAS----------NPSHQQ 846
            |..   |||::....|.|..:||:.|.             |||......          ||   :
Frog   621 PVH---SAPASPFDGNRSRTSSPVRPAKSAVDKTEVSRPGTPNLPVQLKKRCTVLDEELNP---K 679

  Fly   847 QQQHHNQQQQQQQQQQHHPQHHD---NSNSSSNIDP-----LFNYREESND----TSCSQH---L 896
            ....||.|::::.:.. .|.:..   .:...:|:||     |...|.:.:|    :|..|.   |
Frog   680 MVALHNVQRKRKMESD-GPLYQAIGIGTLFQTNVDPGPNSSLLWKRVKGSDSDRPSSAEQRTILL 743

  Fly   897 HSPSITS----KSPEDSSLPSL----CSPNS 919
            .|..:.|    :|.:.:.||.|    |..|:
Frog   744 LSTDMASQLLGQSLDGTVLPQLTGYDCEVNA 774

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sima	NP_001287599.1	HLH	156..209	CDD:238036	34/52 (65%)
		PAS	256..392	CDD:279347	53/146 (36%)
		PAS	265..>317	CDD:238075	22/51 (43%)
		PAS	404..505	CDD:238075	45/102 (44%)
		PAS_3	417..502	CDD:285623	34/86 (40%)
hif1a	XP_031746581.1	bHLH-PAS_HIF1a_PASD8	13..83	CDD:381570	42/79 (53%)
		PAS	93..148	CDD:214512	22/54 (41%)
		PAS_11	241..343	CDD:405306	45/101 (45%)
		HIF-1	549..575	CDD:402842	6/49 (12%)
		HIF-1a_CTAD	761..795	CDD:400914	5/14 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	68	1.000	Domainoid score	I9575
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	336	1.000	Inparanoid score	I2352
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	1	1.000	-	-		otm49414
Panther	1	1.100	-	-	LDO	PTHR23043
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1038
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	7.150