Protein Alignment sima and Hif1a

DIOPT Version :10

• Sequence 1: NP_001287599.1
• Gene: sima / 43580
• FlyBaseID: FBgn0266411
• Length: 1593
• Species: Drosophila melanogaster
• Sequence 2: XP_006240258.1
• Gene: Hif1a / 29560
• RGDID: 61928
• Length: 828
• Species: Rattus norvegicus
• Alignment Length: 900
• Identity: 255/900 - (28%)
• Similarity: 400/900 - (44%)
• Gaps: 221/900 - (24%)

  Fly   154 MNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVP 218
            |::|:||||||||||.|||||:|:|.||:..|||..:..:.||||||||:||::|::|::|
  Rat    14 MSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLL---- 74

  Fly   219 SLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSE 283
               |..|   .|||     .|:|             |:......:.:|||::||:.:||:.|:|:
  Rat    75 ---DAGD---LDIE-----DEMK-------------AQMNCFYLKALDGFVMVLTDDGDMIYISD 115

  Fly   284 NVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRL 348
            ||.:|:|:|:.:..|..:::::|.|||.|::|.|:.:....:|.|::..|        |..|:|:
  Rat   116 NVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQ--------RSFFLRM 172

  Fly   349 KCTLTSRGRSINIKSASYKVIHITGHLVVNAKGER------------LLMAIGRPIPHPSNIEIP 401
            |||||||||::|||||::||:|.|||:.|......            .|:.|..|||||||||||
  Rat   173 KCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIP 237

  Fly   402 LGTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGE 466
            |.:.|||::|||||:|:|.|:::.:|:||.|::||..|::...|..||:.|..|...:.:|||..
  Rat   238 LDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVT 302

  Fly   467 TSRYRFLGKYGGYCWILSQATIVYD--KLKPQSVVCVNYVISNLENKHEIYSLAQQTAASEQKEQ 529
            |.:||.|.|.|||.|:.:|||::|:  ..:||.:||||||:|.:.....|:|| |||
  Rat   303 TGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSL-QQT-------- 358

  Fly   530 HHQAAETEKEPEKAADPEIIAQETKETVNTPIHTSELQAKPL--QLESEKA----EKTIEETKTI 588
                                     |:|..|:.:|:::...|  ::|||..    :|..:|...:
  Rat   359 -------------------------ESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDAL 398

  Fly   589 ATIPPVTA------------TSTAD-QIKQLPESNPYKQILQAELLIKRENHSPGPRTITAQLLS 640
            ..:.|...            |.|.| |::.:|        |..:::....|.........:.|
  Rat   399 TLLAPAAGDTIISLDFGSDDTETEDQQLEDVP--------LYNDVMFPSSNEKLNINLAMSPL-- 453

  Fly   641 GSSSGLRPEEKRPKSVTASVLRPSPAPPLTPPPTAVLCKKTP--LGVEPNLPPTTTATAAIISSS 703
                        |.|.|...||.|..|.|. ...|:..:.:|  ||:...:|......|:....|
  Rat   454 ------------PASETPKPLRSSADPALN-QEVALKLESSPESLGLSFTMPQIQDQPASPSDGS 505

  Fly   704 NQQLQIAQQTQLQNPQQPAQ-------DMSKGFC-----SLFADDGRGLTMLKEEPDDLSHHLAS 756
            .:|       ....|..|::       ||...|.     .|||:|.........:..||...:.:
  Rat   506 TRQ-------SSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLA 563

  Fly   757 TNCIQLD---EMTPFSDMLVGLMGTCLLPEDINSLDSTTCSTTASGQ--HYQSPSSSSTSAPSNT 816
            . .|.:|   ::..|..         |.|.:.||....:.||....|  ..|.|:.:.|:|.:.|
  Rat   564 P-YIPMDDDFQLRSFDQ---------LSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTAATATT 618

  Fly   817 SSSNNS--------------YANSPLSPLTPNSTATASNPSHQQQQQHHNQQQQQ-----QQQQQ 862
            :::.:.              ...||.|...|....||...::...........:.     ::..:
  Rat   619 ATTTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTTSPDRAGKRVIEKTDK 683

  Fly   863 HHPQHHDNSNSSSNIDPLFNYR-----EESNDTSCSQHLHSPSITSKSPEDSSL---PSLCSPNS 919
            .||:       |.|:....|.|     ||.|..:.:  |.:.....|...|.||   ..:.|...
  Rat   684 AHPR-------SLNLSVTLNQRNTVPEEELNPKTIA--LQNAQRKRKMEHDGSLFQAAGIVSGTL 739

  Fly   920 LTQEDDFSFEAFAMRAPYIPIDDDMPLLTETDLMWCPPEDLQTMVPKEIDAIQQQ 974
            |.|..|        |||.:            .|.|   :.::..:..|.|.::|:
  Rat   740 LQQPGD--------RAPTM------------SLSW---KRVKGYISSEQDGMEQK 771

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sima	NP_001287599.1	bHLH-PAS_HIF	159..216	CDD:381439	35/56 (63%)
		PAS	256..392	CDD:395786	51/147 (35%)
		PAS	265..>317	CDD:238075	21/51 (41%)
		PAS	404..505	CDD:238075	46/102 (45%)
Hif1a	XP_006240258.1	bHLH-PAS_HIF1a_PASD8	14..84	CDD:381570	42/84 (50%)
		PAS	94..149	CDD:214512	21/54 (39%)
		PAS_3	255..340	CDD:430001	34/84 (40%)
		HIF-1	551..580	CDD:463274	5/38 (13%)
		HIF-1a_CTAD	791..827	CDD:430212