DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sima and Hif1a

DIOPT Version :9

Sequence 1:	NP_001287599.1	Gene:	sima / 43580	FlyBaseID:	FBgn0266411	Length:	1593	Species:	Drosophila melanogaster
Sequence 2:	NP_001300848.1	Gene:	Hif1a / 15251	MGIID:	106918	Length:	837	Species:	Mus musculus

Alignment Length:	898	Identity:	251/898 - (27%)
Similarity:	406/898 - (45%)	Gaps:	222/898 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   154 MNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVP 218
            |::|:||||||||||.|||||:|:|.||:..|||..:..:.||||||||:||::|::|::|
Mouse    14 MSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLL---- 74

  Fly   219 SLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSE 283
                       |....:.:.|:|.:::.             ...:.:|||::||:.:||:.|:|:
Mouse    75 -----------DAGGLDSEDEMKAQMDC-------------FYLKALDGFVMVLTDDGDMVYISD 115

  Fly   284 NVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRL 348
            ||.:|:|:|:.:..|..:::::|.|||.|::|.|:.:....:|.|:...|        |..|:|:
Mouse   116 NVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQ--------RSFFLRM 172

  Fly   349 KCTLTSRGRSINIKSASYKVIHITGHLVV---NAKGER---------LLMAIGRPIPHPSNIEIP 401
            |||||||||::|||||::||:|.|||:.|   |:...:         .|:.|..|||||||||||
Mouse   173 KCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIP 237

  Fly   402 LGTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGE 466
            |.:.|||::|||||:|:|.|:::.:|:||.|::||..|::...|..||:.|..|...:.:|||..
Mouse   238 LDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVT 302

  Fly   467 TSRYRFLGKYGGYCWILSQATIVYD--KLKPQSVVCVNYVISNLENKHEIYSLAQQTAASEQKEQ 529
            |.:||.|.|.|||.|:.:|||::|:  ..:||.:||||||:|.:.....|:|| |||
Mouse   303 TGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSL-QQT-------- 358

  Fly   530 HHQAAETEKEPEKAADPEIIAQETKETVNTPIHTSELQAKPL--QLESEKA----EKTIEETKTI 588
                                     |:|..|:.:|:::...|  ::|||..    :|..:|...:
Mouse   359 -------------------------ESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDAL 398

  Fly   589 ATIPPVTA------------TSTAD-QIKQLPESNPYKQILQAELLIKRENHSPGPRTITAQLLS 640
            ..:.|...            |.|.| |::.:|        |..:::....|.........:.|
Mouse   399 TLLAPAAGDTIISLDFGSDDTETEDQQLEDVP--------LYNDVMFPSSNEKLNINLAMSPL-- 453

  Fly   641 GSSSGLRPEEKRPKSVTASVLRPSPAPPLTPPPTAVLCKKTP--LGVEPNLP-----PTTTATAA 698
                        |.|.|...||.|..|.|. ...|:..:.:|  ||:...:|     |.:.:..:
Mouse   454 ------------PSSETPKPLRSSADPALN-QEVALKLESSPESLGLSFTMPQIQDQPASPSDGS 505

  Fly   699 IISSSNQQL--QIAQQTQLQNPQQPAQ-------DMSKGFC-----SLFADDGRGLTMLKEEPDD 749
            ...||.::|  :.........|..|::       ||...|.     .|||:|.........:..|
Mouse   506 TRQSSPERLLQENVNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTD 570

  Fly   750 -----LSHHLASTNCIQL---DEMTPFS---------DMLVGLMGTCLLPEDINSLDSTTCSTTA 797
                 |:.::...:..||   |:::|..         ..:.|...|.|....|.:..:||.:|..
Mouse   571 LDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDE 635

  Fly   798 SGQHYQ-----------SPSSS-----STSAPSNTSSSNNSYANSP-------------LSPLTP 833
            |....:           ||||:     :|:|.::..|..:|...||             ..|.:.
Mouse   636 SKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSL 700

  Fly   834 NSTATASNPSHQQQQQHHNQQQQQQQQQQHHPQHHDNS-NSSSNIDPLFN--------------- 882
            |.:||.:..:...:::.:.:....|..|:.....||.| ..::.|..|..
Mouse   701 NLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKR 765

  Fly   883 ----YREESNDTSCSQHLHSPS-----ITSKSPEDSSLPSL----CSPNSLTQ 922
                ...|.|.|.....:..||     :..:|.::|.||.|    |..|:..|
Mouse   766 VKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQ 818

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sima	NP_001287599.1	HLH	156..209	CDD:238036	33/52 (63%)
		PAS	256..392	CDD:279347	52/147 (35%)
		PAS	265..>317	CDD:238075	21/51 (41%)
		PAS	404..505	CDD:238075	46/102 (45%)
		PAS_3	417..502	CDD:285623	35/86 (41%)
Hif1a	NP_001300848.1	HLH	16..69	CDD:238036	33/52 (63%)
		DNA-binding. /evidence=ECO:0000269\|PubMed:26245371	22..31		7/8 (88%)
		PAS	94..149	CDD:214512	21/54 (39%)
		Required for heterodimer formation with ARNT. /evidence=ECO:0000269\|PubMed:26245371	171..192		15/20 (75%)
		PAS	240..339	CDD:238075	42/98 (43%)
		PAS_3	255..340	CDD:285623	34/84 (40%)
		N-terminal VHL recognition site	381..418		5/36 (14%)
		ODD	402..614		42/234 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	493..512		3/18 (17%)
		NTAD	545..589		8/43 (19%)
		HIF-1	565..594	CDD:288296	5/28 (18%)
		C-terminal VHL recognition site	570..586		2/15 (13%)
		ID	590..796		36/205 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	594..685		19/90 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	708..735		2/26 (8%)
		Nuclear localization signal. /evidence=ECO:0000255	729..732		0/2 (0%)
		CTAD	797..837		8/22 (36%)
		HIF-1a_CTAD	800..836	CDD:285931	7/19 (37%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167847531
Domainoid	1	1.000	82	1.000	Domainoid score	I8395
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	1	1.000	-	-		otm44237
orthoMCL	1	0.900	-	-		OOG6_106128
Panther	1	1.100	-	-	LDO	PTHR23043
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4976
SonicParanoid	1	1.000	-	-		X1038
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	9	8.870