Protein Alignment sima and Hif1a

DIOPT Version :10

• Sequence 1: NP_001287599.1
• Gene: sima / 43580
• FlyBaseID: FBgn0266411
• Length: 1593
• Species: Drosophila melanogaster
• Sequence 2: NP_001300848.1
• Gene: Hif1a / 15251
• MGIID: 106918
• Length: 837
• Species: Mus musculus
• Alignment Length: 898
• Identity: 251/898 - (27%)
• Similarity: 406/898 - (45%)
• Gaps: 222/898 - (24%)

  Fly   154 MNNEKRKEKSRDAARCRRSKETEIFMELSAALPLKTDDVNQLDKASVMRITIAFLKIREMLQFVP 218
            |::|:||||||||||.|||||:|:|.||:..|||..:..:.||||||||:||::|::|::|
Mouse    14 MSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLL---- 74

  Fly   219 SLRDCNDDIKQDIETAEDQQEVKPKLEVGTEDWLNGAEARELLKQTMDGFLLVLSHEGDITYVSE 283
                       |....:.:.|:|.:::.             ...:.:|||::||:.:||:.|:|:
Mouse    75 -----------DAGGLDSEDEMKAQMDC-------------FYLKALDGFVMVLTDDGDMVYISD 115

  Fly   284 NVVEYLGITKIDTLGQQIWEYSHQCDHAEIKEALSLKRELAQKVKDEPQQNSGVSTHHRDLFVRL 348
            ||.:|:|:|:.:..|..:::::|.|||.|::|.|:.:....:|.|:...|        |..|:|:
Mouse   116 NVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQ--------RSFFLRM 172

  Fly   349 KCTLTSRGRSINIKSASYKVIHITGHLVV---NAKGER---------LLMAIGRPIPHPSNIEIP 401
            |||||||||::|||||::||:|.|||:.|   |:...:         .|:.|..|||||||||||
Mouse   173 KCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIP 237

  Fly   402 LGTSTFLTKHSLDMRFTYVDDKMHDLLGYSPKDLLDTSLFSCQHGADSERLMATFKSVLSKGQGE 466
            |.:.|||::|||||:|:|.|:::.:|:||.|::||..|::...|..||:.|..|...:.:|||..
Mouse   238 LDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVT 302

  Fly   467 TSRYRFLGKYGGYCWILSQATIVYD--KLKPQSVVCVNYVISNLENKHEIYSLAQQTAASEQKEQ 529
            |.:||.|.|.|||.|:.:|||::|:  ..:||.:||||||:|.:.....|:|| |||
Mouse   303 TGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSL-QQT-------- 358

  Fly   530 HHQAAETEKEPEKAADPEIIAQETKETVNTPIHTSELQAKPL--QLESEKA----EKTIEETKTI 588
                                     |:|..|:.:|:::...|  ::|||..    :|..:|...:
Mouse   359 -------------------------ESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDAL 398

  Fly   589 ATIPPVTA------------TSTAD-QIKQLPESNPYKQILQAELLIKRENHSPGPRTITAQLLS 640
            ..:.|...            |.|.| |::.:|        |..:::....|.........:.|
Mouse   399 TLLAPAAGDTIISLDFGSDDTETEDQQLEDVP--------LYNDVMFPSSNEKLNINLAMSPL-- 453

  Fly   641 GSSSGLRPEEKRPKSVTASVLRPSPAPPLTPPPTAVLCKKTP--LGVEPNLP-----PTTTATAA 698
                        |.|.|...||.|..|.|. ...|:..:.:|  ||:...:|     |.:.:..:
Mouse   454 ------------PSSETPKPLRSSADPALN-QEVALKLESSPESLGLSFTMPQIQDQPASPSDGS 505

  Fly   699 IISSSNQQL--QIAQQTQLQNPQQPAQ-------DMSKGFC-----SLFADDGRGLTMLKEEPDD 749
            ...||.::|  :.........|..|::       ||...|.     .|||:|.........:..|
Mouse   506 TRQSSPERLLQENVNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTD 570

  Fly   750 -----LSHHLASTNCIQL---DEMTPFS---------DMLVGLMGTCLLPEDINSLDSTTCSTTA 797
                 |:.::...:..||   |:::|..         ..:.|...|.|....|.:..:||.:|..
Mouse   571 LDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDE 635

  Fly   798 SGQHYQ-----------SPSSS-----STSAPSNTSSSNNSYANSP-------------LSPLTP 833
            |....:           ||||:     :|:|.::..|..:|...||             ..|.:.
Mouse   636 SKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSL 700

  Fly   834 NSTATASNPSHQQQQQHHNQQQQQQQQQQHHPQHHDNS-NSSSNIDPLFN--------------- 882
            |.:||.:..:...:::.:.:....|..|:.....||.| ..::.|..|..
Mouse   701 NLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKR 765

  Fly   883 ----YREESNDTSCSQHLHSPS-----ITSKSPEDSSLPSL----CSPNSLTQ 922
                ...|.|.|.....:..||     :..:|.::|.||.|    |..|:..|
Mouse   766 VKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQ 818

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sima	NP_001287599.1	bHLH-PAS_HIF	159..216	CDD:381439	35/56 (63%)
		PAS	256..392	CDD:395786	52/147 (35%)
		PAS	265..>317	CDD:238075	21/51 (41%)
		PAS	404..505	CDD:238075	46/102 (45%)
Hif1a	NP_001300848.1	bHLH-PAS_HIF1a_PASD8	14..84	CDD:381570	38/84 (45%)
		DNA-binding. /evidence=ECO:0000269|PubMed:26245371	22..31		7/8 (88%)
		PAS	94..149	CDD:214512	21/54 (39%)
		Required for heterodimer formation with ARNT. /evidence=ECO:0000269|PubMed:26245371	171..192		15/20 (75%)
		PAS_3	255..340	CDD:430001	34/84 (40%)
		N-terminal VHL recognition site	381..418		5/36 (14%)
		ODD	402..614		42/234 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	493..512		3/18 (17%)
		NTAD	545..589		8/43 (19%)
		HIF-1	565..594	CDD:463274	5/28 (18%)
		C-terminal VHL recognition site	570..586		2/15 (13%)
		ID	590..796		36/205 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	594..685		19/90 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	708..735		2/26 (8%)
		Nuclear localization signal. /evidence=ECO:0000255	729..732		0/2 (0%)
		CTAD	797..837		8/22 (36%)
		HIF-1a_CTAD	800..836	CDD:430212	7/19 (37%)