DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Gycalpha99B and Adcy8

DIOPT Version :9

Sequence 1:	NP_477088.2	Gene:	Gycalpha99B / 43493	FlyBaseID:	FBgn0013972	Length:	676	Species:	Drosophila melanogaster
Sequence 2:	NP_033753.2	Gene:	Adcy8 / 11514	MGIID:	1341110	Length:	1249	Species:	Mus musculus

Alignment Length:	704	Identity:	152/704 - (21%)
Similarity:	240/704 - (34%)	Gaps:	263/704 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly    75 NIHKLKLDPQTFKSCANYDYLADIQELLLKMDEASASE------------ILVLLGEELITCCCT 127
            :|.|..||      |.|.||         .::|....|            .|:...||.:.|...
Mouse   548 HISKATLD------CLNGDY---------NVEEGHGKERNEFLRKHNIETYLIKQPEESLLCLPE 597

  Fly   128 GIIERAFRCLGTDLQEFLGSLDGVYDVLKLQEEDVTDTGFVCAGEG----ELIF---TSERPVIA 185
            .|::.:..|                       .|..::|.... ||    ||.|   ..::..:|
Mouse   598 DIVKESVSC-----------------------SDRRNSGATFT-EGSWSPELPFDNIVGKQNTLA 638

  Fly   186 WLLLGSLKALTRMLYKV--------DVNIKIEPV----EGDARRYRYL--FSLV-KDNS------ 229
            .|...|:..|...|.:.        ::|.:||..    .||..|..::  |||: ||:|
Mouse   639 ALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYS 703

  Fly   230 ------------------------QTMLMGRPTSVSKTIPETVQRSNSSNASDLQMNSSSFCKMF 270
                                    |::|   |:  |:.:|.|:|                    |
Mouse   704 QMRDEVFKSNLVCAFIVLLFITAIQSLL---PS--SRLMPMTIQ--------------------F 743

  Fly   271 PWHFIMNEQLELVQLGRGFSKLYKPYMADFGCQATTYFDFKRPKGLTMKFRDIVRRTYTP----- 330
            ....:::..|.|:                     ||..|:|....:..|....:..||..
Mouse   744 SILIMLHSALVLI---------------------TTAEDYKCLPLILRKTCCWINETYLARNVII 787

  Fly   331 FLIGLNNPPGAV------DF----PAIGLEIKGQMVH---CPESNSLLFIGSPFLDGLDGLTC-- 380
            |...|.|..|||      ||    |...|......|.   |......:|.|.     |..:||
Mouse   788 FASILINFLGAVLNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGV-----LAMVTCAV 847

  Fly   381 ----------------------------NGLFISDIPLHDA------TREVILV----------- 400
                                        .|||:|...|:.:      |:|..|:
Mouse   848 FLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAVFY 912

  Fly   401 -GEQ----ARAQDGLRRRMDKIKNSIEEANSAVTKE-RKKNVSLLHLIFPAEIA----EKLWLGS 455
             |:|    ||. |.|.|...|     ||.|.  .|| |:.|.::|..|.|:.:|    ||.....
Mouse   913 HGQQLEYTARL-DFLWRVQAK-----EEINE--MKELREHNENMLRNILPSHVARHFLEKDRDNE 969

  Fly   456 SIDAKTYPDVTILFSDIVGFTSICSRA----TPFMVISMLEGLYKDFDEFCD---FFDVYKVETI 513
            .:.:::|..|.::|:.|.||....|:.    .....:.:|..:..||||...   |.|:.|::||
Mouse   970 ELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTI 1034

  Fly   514 GDAYCVASGL------------HRASIYDAHKVAWMALKMIDACSKHITHDGEQIKMRIGLHTGT 566
            |..|...|||            |..::.|      .:|.:.::..:...|.....::|||:..|:
Mouse  1035 GSTYMAVSGLSPEKQQCEDKWGHLCALAD------FSLALTESIQEINKHSFNNFELRIGISHGS 1093

  Fly   567 VLAGVVGRKMPRYCLFGHSVTIANKFESGSEALKINVSPTTKDWLTKHEGFEFE 620
            |:|||:|.|.|:|.::|.:|.:|::.:|...:.:|.| |.....:.|.:||.|:
Mouse  1094 VVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQV-PEETYLILKDQGFAFD 1146

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gycalpha99B	NP_477088.2	HNOB	76..208	CDD:285002	29/158 (18%)
		HNOBA	259..451	CDD:285003	55/266 (21%)
		CYCc	430..619	CDD:214485	58/212 (27%)
		Guanylate_cyc	457..647	CDD:278633	49/183 (27%)
Adcy8	NP_033753.2	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..180
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..107
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	14..33
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..118
		AC_N	<159..398	CDD:292831
		CYCc	364..562	CDD:214485	8/28 (29%)
		Guanylate_cyc	403..587	CDD:278633	11/53 (21%)
		DUF1053	615..709	CDD:283888	22/94 (23%)
		CYCc	942..1146	CDD:214485	56/210 (27%)
		Guanylate_cyc	971..1170	CDD:278633	49/183 (27%)
		Involved in CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1107..1249		11/41 (27%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000250\|UniProtKB:P40146	1198..1213
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1221..1249
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900