DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment rdog and Cftr

DIOPT Version :9

Sequence 1:	NP_651678.1	Gene:	rdog / 43450	FlyBaseID:	FBgn0039644	Length:	1374	Species:	Drosophila melanogaster
Sequence 2:	NP_066388.1	Gene:	Cftr / 12638	MGIID:	88388	Length:	1476	Species:	Mus musculus

Alignment Length:	1502	Identity:	394/1502 - (26%)
Similarity:	687/1502 - (45%)	Gaps:	273/1502 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 KNPFTKANFFSQWSFWWMRDLFKRGLQGPLTDEELYQHRKTLDSERVTNKFAELWE-DELKRSNP 75
            |:|..||:|.|:..|.|...:.::|.:..|...::||......::.::.|....|: ::..:.||
Mouse     3 KSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKKNP 67

  Fly    76 SVVRMILRAYGKIFVPMGLAFSISETICKSLMPLFLGGLV-GYFATNQTDISENTAYLYAMGIVI 139
            .::..:.|.:...|:..|:...:.| :.|::.|:.||.:: .|...|:.   |.:..:| :||.:
Mouse    68 QLIHALRRCFFWRFLFYGILLYLGE-VTKAVQPVLLGRIIASYDPENKV---ERSIAIY-LGIGL 127

  Fly   140 CML--VPVITFHPFIFYIFQVGTKLRLALSGLIYRKVLQVSKSSSNDGLRGRAINILSNDLGRFD 202
            |:|  |..:..||.||.:.::|.::|.|:..|||:|.|::|....:....|:.:::|||:|.:||
Mouse   128 CLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFD 192

  Fly   203 VALCFLHDTWKGPMESILIGFLMYREIGISAVIGVSFMLSFIPLQAWAAKKAAYYRQMTAERTDM 267
            ..|...|..|..|::..|:..|::..:..||..|:..::..:..||...|....||...|.:.:.
Mouse   193 EGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINE 257

  Fly   268 RVKLMNEIIQGIQVIKMYAWEKSFARVIAEVRLKEVKAIRGTAYIHAALGCTSMISP-LSVFLAL 331
            |:.:.:|||..|..:|.|.||.:..::|..:|..|:|..|..||:..........|. ..|||::
Mouse   258 RLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFVVFLSV 322

  Fly   332 CSYVYLGDPLTAQKVYTVSSYFNMLNDSMVTFWPMSITFIAEALVSAKRCKDFLLDGDKAEVPIN 396
            ..|..: :.:..:|::|..|:..:|..|:...:|.::....::....::.:|||
Mouse   323 LPYTVI-NGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFL----------- 375

  Fly   397 LEANQQTGKNKRKISKKDKQKNVELNGSLLSNGQVHHNRLRDYDPEATKKCVVLKNVSASWDTSD 461
                           :|.:.|.:|.|  |::.|                  ::::||:|.|:...
Mouse   376 ---------------QKQEYKVLEYN--LMTTG------------------IIMENVTAFWEEGF 405

  Fly   462 GH-----------------------------ANCAIEDFSTDIKDQTLAAVVGPVGAGKSSFLNV 497
            |.                             .|..:::.:.:|:...:.|:.|..|:||:|.|.:
Mouse   406 GELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLNIEKGEMLAITGSTGSGKTSLLML 470

  Fly   498 LLGEVGIDKGEAMVHGKISYASQESWVFEGTIRDNIVFVEDYNERRYKKVVKVCGLERDMELLPR 562
            :|||:...:|.....|::|:.||.||:..|||::||:|...|:|.|||.|||.|.|::|:.....
Mouse   471 ILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAE 535

  Fly   563 GDLTVVGERGVSLSGGQKARVSLARAVYRKADIYLLDDPLSAVDTHVGKHIFDRCIRDFLSNKIR 627
            .|.||:||.||:|||||:||:|||||||:.||:||||.|...:|....:.:|:.|:...::||.|
Mouse   536 QDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKTR 600

  Fly   628 ILVTHQVQYLFDVEHLLLMGSGKIMAQGSYQELQRSR-QFQ--------FLEQTQHDESGIDTHS 683
            ||||.::::|...:.:|::..|.....|::.|||..| .|.        |.:.|:...|.|.|.:
Mouse   601 ILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTET 665

  Fly   684 VSSYLSRSDSEKSM--------------EHQHNQLLRPDEHVEDVNQEQQT-------------- 720
            :..: |..||....              |.:.|.:|.....|..::..|:|
Mouse   666 LRRF-SVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNSFSSVRKISIVQKTPLCIDGESDDLQEK 729

  Fly   721 --------------------------------------------------------------VGS 723
                                                                          |..
Mouse   730 RLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQ 794

  Fly   724 IKMHVYASYIKAL--DST-----------------------------------FLL--GIVISLF 749
            |.::....|.:.|  |||                                   |.|  |:::.|.
Mouse   795 ISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLHKGLLLVLI 859

  Fly   750 VCARIMLTGVDYFLSRWVIWEEQIAVNRTTTLLNETDTTIANDTLPINATDPYIAPLLASDIQAG 814
            .|..:.|  |:...|.:|:|           ||........|:...| :...|:..:.::..
Mouse   860 WCVLVFL--VEVAASLFVLW-----------LLKNNPVNSGNNGTKI-SNSSYVVIITSTSF--- 907

  Fly   815 IRQELVLFYATILGATLIVYLIRTFGFFKMCLRISLHLHDRLFRGITRASMYFFNTNSSGRILNR 879
              ..:...|..:....|.:.|.|........:..|..||.::...|..|.|...:...:|.||||
Mouse   908 --YYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTISKLKAGGILNR 970

  Fly   880 FSKDIRTVDTDLPHTLLDCLAFIIDVSGVVIIV-AIANYWLL--IPAAIIGVILGMIRYLYVNTS 941
            |||||..:|..||.|:.|.:..:..|.|.:|:| |:..|..|  :|..::.::|   |..:::|:
Mouse   971 FSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFILL---RAYFLHTA 1032

  Fly   942 RSVKRLESISRSPVFSLTNQTFQGLTTIRALQAQSALEQEFHEYQNTNTSAWFLFLSCTRAFALW 1006
            :.:|:|||..|||:|:....:.:||.|:||.:.|:..|..||:..|.:|:.||::|:..|.|.:.
Mouse  1033 QQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMR 1097

  Fly  1007 SDVLCIVYMTAVTFSFLLLKDEFDSGDVGLAILHSTTMTGMCQWGMRQTAELENQMTSVERVLE- 1070
            .|::.:::...|||..:|...| ..|..|:.:..:..:....||.:..:.:.::.|.||.||.:
Mouse  1098 IDMIFVLFFIVVTFISILTTGE-GEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKF 1161

  Fly  1071 ---------YT--------EQPSEALLETPEKFKPKSEWPSKGRIEFINFKLRYSPKEAPVLKDL 1118
                     ||        |..|:.|:...|..|....|||.|.:...:..::|......||:::
Mouse  1162 IDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDDGNAVLENI 1226

  Fly  1119 NFTIEPREKIGIVGRTGAGKSSIIQSIFRLACNEGMIRIDDVDIEHIGLHDLRSQVSIIPQDPVL 1183
            :|:|.|.:::|::||||:|||:::.:..|:...:|.|.||.|....:.|.:.|....:|.|...:
Mouse  1227 SFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKAFGVITQKVFI 1291

  Fly  1184 FSGTLRYNLDPMDERSDEEMWKALGDVELRSYVSTLIGGLNCRMYDGGSNFSVGQRQLVCLARAI 1248
            ||||.|.||||..:..|||:||...:|.|:|.:....|.||..:.|||...|.|.:||:||||::
Mouse  1292 FSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSV 1356

  Fly  1249 LRHNKILIMDEATANVDPETDKLIQQTIRSKFAHCTVLTIAHRLHTVMDSDRVLVMDAGEVRELG 1313
            |...||:::||.:|::||.|.::|::.::..||.|||:...||:..::|..|.||::...|.:
Mouse  1357 LSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLVIEESNVWQ-- 1419

  Fly  1314 HPYELLQ 1320
              |:.||
Mouse  1420 --YDSLQ 1424

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
rdog	NP_651678.1	CFTR_protein	12..1343	CDD:273530	394/1502 (26%)
		ABC_membrane	94..360	CDD:294371	74/269 (28%)
		ABCC_MRP_domain1	448..650	CDD:213217	81/230 (35%)
		ABC_membrane	821..1046	CDD:294371	68/227 (30%)
		ABCC_MRP_domain2	1096..1315	CDD:213211	78/218 (36%)
Cftr	NP_066388.1	CFTR_protein	1..1464	CDD:273530	394/1502 (26%)
		ABC_membrane	85..303	CDD:294371	66/222 (30%)
		ABCC_CFTR1	389..670	CDD:213258	94/299 (31%)
		CFTR_R	639..844	CDD:291094	22/205 (11%)
		Disordered R region. /evidence=ECO:0000250\|UniProtKB:P13569	654..826		20/172 (12%)
		ABC_membrane	861..1142	CDD:279056	81/303 (27%)
		ABCC_CFTR2	1204..1464	CDD:213256	81/225 (36%)
		Interaction with GORASP2. /evidence=ECO:0000250\|UniProtKB:P13569	1382..1476		15/47 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1446..1476
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P13569	1474..1476
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167841716
Domainoid	1	1.000	58	1.000	Domainoid score	I10724
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.850