Protein Alignment Pkc98E and PKN2

DIOPT Version :10

• Sequence 1: NP_524545.2
• Gene: Pkc98E / 43428
• FlyBaseID: FBgn0003093
• Length: 739
• Species: Drosophila melanogaster
• Sequence 2: NP_006247.1
• Gene: PKN2 / 5586
• HGNCID: 9406
• Length: 984
• Species: Homo sapiens
• Alignment Length: 702
• Identity: 229/702 - (32%)
• Similarity: 330/702 - (47%)
• Gaps: 146/702 - (20%)

  Fly    52 DRATTRPKTFDPV----WNEQFVHDVTNVSNINLTVFHDAALPPDDFVANCIISF---EDLMQSE 109
            |.......::.|:    |:::|..::.....:.::|:.      .|:.:.|.:.|   ||.:.::
Human   400 DNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYW------RDWRSLCAVKFLRLEDFLDNQ 458

  Fly   110 TAVQDLWVNLEPQGKIHVIIELKNRTDKAKAEAVVEHTVAVNKEFKERAGFNRRRGAMRRRVHQV 174
            .  ..:.:.|||||.:...:...|        .|:|....:.::.|.   |::::|
Human   459 R--HGMCLYLEPQGTLFAEVTFFN--------PVIERRPKLQRQKKI---FSKQQG--------- 501

  Fly   175 NGHKFMATFLRQPTFCSHCREFIWGIGKQGYQCQVCTLVVHKKCHLSVVS-KCPGMRDEQPAKVE 238
                  .||||.|..  :.....||        ::....:....|....| :.|     .|..|.
Human   502 ------KTFLRAPQM--NINIATWG--------RLVRRAIPTVNHSGTFSPQAP-----VPTTVP 545

  Fly   239 MVPAGQRFNVNLPHRFVVHSYKRFTFCDHCGSLLYGLIKQGLQCETCGMNVHKRCQKNVANTCGI 303
            :|      :|.:|......|....|..|.
Human   546 VV------DVRIPQLAPPASDSTVTKLDF------------------------------------ 568

  Fly   304 NTKQMAEILSSLGISPDKQQPRRSKYLNQQGGEDNYGASL------GADGDGA-PGQSFRSCALS 361
                      .|...|....||.|..     ||.:..:.|      |.|.:.. ..|:.|:..|.
Human   569 ----------DLEPEPPPAPPRASSL-----GEIDESSELRVLDIPGQDSETVFDIQNDRNSILP 618

  Fly   362 VDSLATSTTTMTSGYNSSSCMSLAVTGSGGVGATGETRPG----KCSLLDFNFIKVLGKGSFGKV 422
            .........|..||...|....|            |.|..    :.:|.||....|||:|.||||
Human   619 KSQSEYKPDTPQSGLEYSGIQEL------------EDRRSQQRFQFNLQDFRCCAVLGRGHFGKV 671

  Fly   423 MLAEKKGTDEIYAIKVLKKDAIIQDDDVDCTMTEKRILAL--AANHPFLTALHSCFQTPDRLFFV 485
            :|||.|.|:|::|||.|||..|:..|:||..|.||||...  :..||||..|.:||||.:.:.||
Human   672 LLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFV 736

  Fly   486 MEYVNGGDLMFQIQKARRFEASRAAFYAAEVTLALQFLHTHGVIYRDLKLDNILLDQEGHCKLAD 550
            |||..|||||..|. ...|...||.||||.|.|.||:||.|.::||||||||:|||.||..|:||
Human   737 MEYAAGGDLMMHIH-TDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIAD 800

  Fly   551 FGMCKEGIMNGMLTTTFCGTPDYIAPEILKEQEYGASVDWWALGVLMYEMMAGQPPFEADNEDEL 615
            ||:||||:..|..|:||||||:::|||:|.|..|..:||||.||||:|||:.|:.||..|:|:|:
Human   801 FGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEV 865

  Fly   616 FDSIMHDDVLYPVWLSREAVSILKGFLTKNPEQRLGCT-GDENEIRKHPFFAKLDWKELEKRNIK 679
            ||||::|:|.||.:||.||:||::..|.:|||:|||.: .|..:::|||||..:||..|..:.:|
Human   866 FDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVKKHPFFRLIDWSALMDKKVK 930

  Fly   680 PPFRPKMKNPRDANNFDAEFTKEDPVLTPIGNEVVRCINQDE---FAGFSFV 728
            |||.|.::...|.:|||.|||.|.|:|||  ....|.::::|   |..|.::
Human   931 PPFIPTIRGREDVSNFDDEFTSEAPILTP--PREPRILSEEEQEMFRDFDYI 980

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pkc98E	NP_524545.2	C2_PKC_epsilon	1..134	CDD:175981	15/88 (17%)
		C1_nPKC_epsilon-like_rpt1	171..230	CDD:410385	10/59 (17%)
		C1_nPKC_epsilon-like_rpt2	250..304	CDD:410388	4/53 (8%)
		STKc_nPKC_epsilon	412..729	CDD:270743	167/323 (52%)
PKN2	NP_006247.1	HR1_PKN_1	42..107	CDD:212012
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	114..133
		HR1	130..201	CDD:469609
		HR1_PKN2_3	209..282	CDD:212025
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	351..383
		Necessary to rescue apical junction formation. /evidence=ECO:0000250	382..463		10/70 (14%)
		C2_PKN-like	390..473	CDD:176069	15/80 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	558..584		8/71 (11%)
		STKc_PKN	657..982	CDD:270741	168/327 (51%)
		Necessary for the catalytic activity	917..977		23/61 (38%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA	978..984		0/3 (0%)