DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Doa and Hipk2

DIOPT Version :9

Sequence 1:	NP_001014681.2	Gene:	Doa / 43415	FlyBaseID:	FBgn0265998	Length:	2045	Species:	Drosophila melanogaster
Sequence 2:	NP_034563.2	Gene:	Hipk2 / 15258	MGIID:	1314872	Length:	1196	Species:	Mus musculus

Alignment Length:	560	Identity:	148/560 - (26%)
Similarity:	240/560 - (42%)	Gaps:	143/560 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly  1561 MQPVKYQQQQQH-----PHTQFQNASAGGGGGGAAGLQYDPNTNTQLF-YASPA---SSSSNKQP 1616
            |.|| |:....|     |||...:|..     ....|:.:|::|..:. |.|.:   |.|.|..|
Mouse     1 MAPV-YEGMASHVQVFSPHTLQSSAFC-----SVKKLKVEPSSNWDMTGYGSHSKVYSQSKNIPP 59

  Fly  1617 QQPQQQQQQ-----QQSQLQQSNSVIFNHS---------------GQ-QHQPHQ----------- 1649
            .||......     ....|....::||..|               || ...||.
Mouse    60 SQPASTTVSTSLPIPNPSLPYEQTIIFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLD 124

  Fly  1650 -------QQQNEMSKSALGLHFIETAKPVIQDDADG------------------------HLIYH 1683
                   ::::|..::...:..||...|:||::|.|                        .|:.|
Mouse   125 TYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQH 189

  Fly  1684 TGDIL---HHRYKIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYREAAKLEINALEKIAQ 1745
              ::|   .:.|:::..||.||||:|||......:..:|:||:||...|....::|::.|.:::.
Mouse   190 --EVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLST 252

  Fly  1746 KDPHCDHLCVKMIDWFDYHGHMCIVFEMLGLSVFDFLRENNYEPYPLDQVRHMAYQLCYSVKFLH 1810
            :... |:..|:..:.|.:..|.|:|||||..:::|||::|.:.|.||..:|.:..|:..::..|.
Mouse   253 ESAD-DYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLK 316

  Fly  1811 DNRLTHTDLKPENILFVDSDYTSHYNHKINREVRRVKNTDVRLIDFGSATFDHEHH------STI 1869
            ...|.|.|||||||:.||.          :|:..|||     :||||||:     |      ||.
Mouse   317 SLGLIHADLKPENIMLVDP----------SRQPYRVK-----VIDFGSAS-----HVSKAVCSTY 361

  Fly  1870 VSTRHYRAPEVILELGWSQPCDVWSIGCILFELYLGITLFQTHDNREHLAMMERILGQIPYRMAR 1934
            :.:|:|||||:||.|.:.:..|:||:||::.||:||..|:......:.:..:.:..|     :..
Mouse   362 LQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQG-----LPA 421

  Fly  1935 NHTLYSKTKTKYFYHGKLD-----W------DEKSSAGRYVRDHCKPLFLC-------------- 1974
            .:.|.:.|||..|::...|     |      |.::..|...::..|.:|.|
Mouse   422 EYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLE 486

  Fly  1975 ---QLSDSEDHCELFSLIKKMLEYEPSSRITLGEALHHPF 2011
               .|.:..|..|...|:||||..:...|:|..|.|:|||
Mouse   487 GSDMLVEKADRREFIDLLKKMLTIDADKRVTPIETLNHPF 526

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Doa	NP_001014681.2	PKc_CLK	1679..2012	CDD:271036	112/370 (30%)
Doa	NP_001014681.2	S_TKc	1692..2012	CDD:214567	109/354 (31%)
Hipk2	NP_034563.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..69		6/18 (33%)
		Transcriptional corepression	97..230		27/134 (20%)
		STKc_HIPK2	183..537	CDD:271129	112/372 (30%)
		Interaction with DAXX. /evidence=ECO:0000250	189..520		106/358 (30%)
		S_TKc	199..527	CDD:214567	109/354 (31%)
		Interaction with SKI and SMAD1. /evidence=ECO:0000250	539..844
		Interaction with POU4F1. /evidence=ECO:0000269\|PubMed:15492043	752..897
		Interaction with CTBP1. /evidence=ECO:0000269\|PubMed:14567915	774..876
		Interaction with HMGA1. /evidence=ECO:0000269\|PubMed:11593421	787..897
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	792..847
		Nuclear localization signal 1 (NLS1). /evidence=ECO:0000250	802..805
		Nuclear localization signal 2 (NLS2). /evidence=ECO:0000250	832..835
		Interaction with TP53 and TP73. /evidence=ECO:0000269\|PubMed:11780126	839..934
		Localization to nuclear speckles	873..980
		Required for localization to nuclear speckles. /evidence=ECO:0000250	873..980
		Interaction with UBE2I. /evidence=ECO:0000269\|PubMed:10535925	873..907
		SUMO interaction motifs (SIM), required for nuclear localization and kinase activity. /evidence=ECO:0000250	884..908
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	891..963
		Interaction with AXIN1. /evidence=ECO:0000269\|PubMed:15526030	935..1050
		Autoinhibitory domain (AID). /evidence=ECO:0000250	984..1196
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	991..1058
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0667
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810