DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Doa and Hipk1

DIOPT Version :9

Sequence 1:	NP_001014681.2	Gene:	Doa / 43415	FlyBaseID:	FBgn0265998	Length:	2045	Species:	Drosophila melanogaster
Sequence 2:	NP_001288233.1	Gene:	Hipk1 / 15257	MGIID:	1314873	Length:	1210	Species:	Mus musculus

Alignment Length:	575	Identity:	146/575 - (25%)
Similarity:	249/575 - (43%)	Gaps:	112/575 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly  1481 PLTHSASFSSAQR----PTALQFHQQHQQQQHLQQQQQHPQQQQHQHSSFGVGMMSRNYYNMPKQ 1541
            |...|::|.||::    |:......|....::....:..|..|....||..|.     .:|:|
Mouse    11 PSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQASSSHQVA-----NFNLP-- 68

  Fly  1542 PERKPLQTFDPYAYPKPNQMQPVKYQQQQQHPHTQFQNASAGGGGGGAAGLQYDPNTNTQLFYAS 1606
                        ||.: ..:.|.        |..:....:|....|.||         |..|.:|
Mouse    69 ------------AYDQ-GLLLPA--------PAVEHIVVTAADSSGSAA---------TATFQSS 103

  Fly  1607 PA-SSSSNKQPQQPQQQ--QQQQQSQLQQSNSVIFNHSGQQHQPHQQQQNEMSKSALGLHFIETA 1668
            .. :..||....:|.|:  .:::..:::.:.||   ...::|.|...|...:..:|.....:.|.
Mouse   104 QTLTHRSNVSLLEPYQKCGLKRKSEEVESNGSV---QIIEEHPPLMLQNRTVVGAAATTTTVTTK 165

  Fly  1669 KPVIQDDADGHLIYHTGDIL---HHRYKIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYR 1730
            ......:.|..|:.|  :||   .:.|:::..||.||||:|.|.........:|:||:||...|.
Mouse   166 SSSSSGEGDYQLVQH--EILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYA 228

  Fly  1731 EAAKLEINALEKIAQKDPHCDHLCVKMIDWFDYHGHMCIVFEMLGLSVFDFLRENNYEPYPLDQV 1795
            ...::|::.|.:::.::.. ::..|:..:.|.:..|.|:|||||..:::|||::|.:.|.||..:
Mouse   229 RQGQIEVSILSRLSSENAD-EYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYI 292

  Fly  1796 RHMAYQLCYSVKFLHDNRLTHTDLKPENILFVDSDYTSHYNHKINREVRRVKNTDVRLIDFGSAT 1860
            |.:..|:..::..|....|.|.|||||||:.||.          .|:..|||     :||||||:
Mouse   293 RPILQQVATALMKLKSLGLIHADLKPENIMLVDP----------VRQPYRVK-----VIDFGSAS 342

  Fly  1861 FDHEHH------STIVSTRHYRAPEVILELGWSQPCDVWSIGCILFELYLGITLFQTHDNREHLA 1919
                 |      ||.:.:|:|||||:||.|.:.:..|:||:||::.||:||..|:......:.:.
Mouse   343 -----HVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIR 402

  Fly  1920 MMERILGQIPYRMARNHTLYSKTKTKYFYH-----GKLDW------DEKSSAGRYVRDHCKPLFL 1973
            .:.:..|     :...:.|.:.|||..|::     |...|      :.:...|...::..|.:|.
Mouse   403 YISQTQG-----LPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFN 462

  Fly  1974 C-----------------QLSDSEDHCELFSLIKKMLEYEPSSRITLGEALHHPF 2011
            |                 .|::..|..|...|:||||..:...|||..:.|:|.|
Mouse   463 CLDDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQF 517

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Doa	NP_001014681.2	PKc_CLK	1679..2012	CDD:271036	109/370 (29%)
Doa	NP_001014681.2	S_TKc	1692..2012	CDD:214567	105/354 (30%)
Hipk1	NP_001288233.1	STKc_HIPK1	174..528	CDD:271130	110/372 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	835..856
		Nuclear localization signal 1 (NLS1). /evidence=ECO:0000250	844..847
		Interaction with TP53. /evidence=ECO:0000269\|PubMed:12702766	885..1093
		Required for localization to nuclear speckles. /evidence=ECO:0000250	891..998
		SUMO interaction motifs (SIM), required for nuclear localization and kinase activity. /evidence=ECO:0000250	902..926
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	938..981
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1046..1069
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1084..1104
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0667
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.900