DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spg and Dock1

DIOPT Version :9

Sequence 1:	NP_733258.4	Gene:	spg / 43404	FlyBaseID:	FBgn0264324	Length:	2187	Species:	Drosophila melanogaster
Sequence 2:	NP_001028592.1	Gene:	Dock1 / 330662	MGIID:	2429765	Length:	1865	Species:	Mus musculus

Alignment Length:	2123	Identity:	735/2123 - (34%)
Similarity:	1080/2123 - (50%)	Gaps:	343/2123 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 WIPS--NNKYGVAIHNWHGDVRFGLALDVGDSVDIVEECKHWYRGSCPRKSRAVGLFPKTYIHIK 64
            |:|:  ..|||||.:|:.......|:|.:||:|.|:|..:.||||...||....|:||.:|||:|
Mouse     4 WVPTKREEKYGVAFYNYDARGADELSLQIGDTVHILETYEGWYRGYTLRKKSKKGIFPASYIHLK 68

  Fly    65 ----------------DLSKIDPVVAECTQVLREWSEIWKRLYVDREAYKFQTLRKVMFSILESR 113
                            ||    |::.|.|..|||||.||::|||......|:::|.:::.::|.|
Mouse    69 EAIVEGKGQHETVIPGDL----PLIQEVTTTLREWSTIWRQLYVQDNREMFRSVRHMIYDLIEWR 129

  Fly   114 RELLSATMTQDQTLELQMVVVSKIDWGNRKLGLDLVPR-EGPLAVDPHGIGIVKLYNVHVSSADN 177
            .::||.|:.||:..||:..|.:|||:|||.|.||||.| |....:||.....:.|:..|..::..
Mouse   130 SQILSGTLPQDELKELKKKVTAKIDYGNRILDLDLVVRDEDGNILDPELTSTISLFRAHEVASKQ 194

  Fly   178 A-----KASSSRGTLRRKTQRKIL---THHLYFCMRDFGHRIGGDDAEVYFFLYDGSHMRPLSER 234
            .     :..|.:..:....|.|..   :..|:..:::...:| |:||||...|||....:.:||.
Mouse   195 VEERLQEEKSQKQNMDINRQAKFAATPSLALFVNLKNVVCKI-GEDAEVLMSLYDPMESKFISEN 258

  Fly   235 FLVKISKDGFSNYIERLHSNCTVFTDLGAADL-NEELHLVAVVMRVGKIIQSDSIKKIEKSGTLG 298
            :||:.|..|....|:|||:...||||||:.|| .|::..|..::|||::...||..:...||
Mouse   259 YLVRWSSSGLPKDIDRLHNLRAVFTDLGSKDLKREKISFVCQIVRVGRMELRDSNTRKLTSG--- 320

  Fly   299 HGPTFRRPFGVGVLSLGDIAHFDSSLEQSSDEREYNFKLFQ--CEEKDFHLLPELIIKKSSGKYS 361
                .||||||.|:.:.||.:     .:..||.:.:|..||  ..|.||  |..:|.|..:.|
Mouse   321 ----LRRPFGVAVMDVTDIIN-----GKVDDEDKQHFIPFQAVAGENDF--LQTVINKVIAAK-- 372

  Fly   362 PIQASQGTQGIVVSLKLLHGGLGQARQEQPMLFQGST-ITRKMGFPDVIMPGDVRNDLFLNLERG 425
              :.:...||:.|:||||.|.:.|.|:|.|.|...:| :.||.|||::|||||||||:::.|.:|
Mouse   373 --EVNHKGQGLWVTLKLLPGDIHQIRKEFPHLVDRTTAVARKTGFPEIIMPGDVRNDIYVTLVQG 435

  Fly   426 EFERGGKNTGKNILVTVVVLDVAGNVLTDCLWGASGMESQPQYRSMILYHQNAPSWNEMLRLSVP 490
            :|::|.|.|.||:.|||.|.|..|..|...::..:|.|:..:|:|:|.|....|.|.|.|::::|
Mouse   436 DFDKGSKTTAKNVEVTVSVYDEDGKRLEHVIFPGAGDEAISEYKSVIYYQVKQPRWFETLKVAIP 500

  Fly   491 IDKFSTAHVRFEFRHCS---TRDKTEPKLFAFSFARLMDTGGATLGDGLHELYVYKCEDPAKLQV 552
            |:..:.:|:||.|||.|   ::||:| |:||.:|.:||...|.||.||.|:|.|||.|.......
Mouse   501 IEDVNRSHLRFTFRHRSSQDSKDKSE-KIFALAFVKLMRYDGTTLRDGEHDLIVYKAEAKKLEDA 564

  Fly   553 ANYLRLQCRPRDAQAK---------MDCGGCFSRSSKEVFVLRSLLCSTKLTQNADLLSLLQWRT 608
            |.||.|.....:.:.|         ...|.|  ..||:.|.:.:|:||||||||.|||.||:||:
Mouse   565 ATYLSLPSTKGELEEKGHSATGKGMQSLGSC--TISKDSFQISTLVCSTKLTQNVDLLGLLKWRS 627

  Fly   609 YPETIQDSLTGVLRLNDEELVKFLQDVLDALF-AMFSNEEGNSTQHSGLVFHVLVSIFSLLQSNK 672
            ....:|.:|..:::::..|:||||||.||||| .|..|.|  |.....|||..||.|..|:...|
Mouse   628 NTNLLQQNLRQLMKVDGGEVVKFLQDTLDALFNIMMENSE--SETFDTLVFDALVFIIGLIADRK 690

  Fly   673 FQHFRPVMNEYIENHFAAALVYKGLITSVEHMAVFMTKAEHP---DPFQKCFGSLEYIFKLLIQS 734
            ||||.||:..||:.||:|.|.|..| |.|  :..::..||.|   :...|...:||||||.:::|
Mouse   691 FQHFNPVLETYIKKHFSATLAYTKL-TKV--LRTYVASAEKPGVNEQLYKAIKALEYIFKFIVRS 752

  Fly   735 RKLFARATGGQYEDSFKRDLHSLFTALNGMLAVPSYDVIIPTQEALLNSTGVVLEQLKDTLPAPE 799
            |.||.:....:.|..|...|..||.::|.|::..| ::.:..:.|.|.....::..:|......|
Mouse   753 RVLFNQLYENKGEADFVESLLQLFRSINDMMSSLS-ELTVRVKGAALKYLPTIVNDVKLVFDPKE 816

  Fly   800 LGMLARNMLDAIPRGAPIRLIQAKLHAVKDLVSGELFHEDDSRTVVLSVACKHLRMHLSRRDELR 864
            |..:....:..:|.|.   |...||..:.::|..:||.:.|.|.::|.:....|:.||.|::||.
Mouse   817 LSKMFTEFILNVPAGL---LTVQKLSCLIEIVHSDLFTQHDCREILLPMMTDQLKYHLERQEELE 878

  Fly   865 LCAEILSEILSQLYDLQKEQREKVTNTLQHDLDSLCKNILGILIRTIS---IIMEGSNAVLPQLV 926
            .|.::||.||..||      |:.|..|.:|     .:.|:..|:||::   |.|...:.::...|
Mouse   879 ACCQLLSNILEVLY------RKDVGPTQRH-----VQIIMETLLRTVNRTVISMGRDSELIGNFV 932

  Fly   927 SCLLGLLQLLDETHY----KRYWDELSPNKDPRDLKEFLSKSLLVYEELLTQDWHVFPNDWLVMK 987
            :|:..:|:.:::.||    |.:      .|...|:.:||.::.::::.|:.:  :|:|.||::|.
Mouse   933 ACMTAILRQMEDYHYAHLIKTF------GKMRTDVVDFLMETFIMFKNLIGK--NVYPFDWVIMN 989

  Fly   988 LAANDVLRMSLEEFAKPLVYRFLGPQAFDSQLWWSYFSLAVAFLTQPSLQLEQYREPKRLKILHS 1052
            ...|.|...::.::|..|..|||....|:.|||.:||.||||||||.|||||.:...||.|||:.
Mouse   990 TMQNKVFLRAINQYADMLNKRFLDQANFELQLWNNYFHLAVAFLTQESLQLENFSSAKRAKILNK 1054

  Fly  1053 HGDMRVLMGFQILSMWSQLGEQKLHFIPSMVGPFLEVTLVPEPALRKATLSVFYDMMQCEQAARG 1117
            :||||..:||:|..||..||:.|:.|||.||||.||:||:||..||||||.:|:||||||..:..
Mouse  1055 YGDMRRQIGFEIRDMWYNLGQHKIKFIPEMVGPILEMTLIPETELRKATLPIFFDMMQCEFHSTR 1119

  Fly  1118 SFRLVESELIDKLDLLISENKGDDEYRELFSTILLEKVQVENPNWKEAGIAFIASVTRLLERLLD 1182
            ||::.|:|:|.|||..:...:||::|:.||..||||..: ::....:.|..|:..|.||:|||||
Mouse  1120 SFQMFENEIITKLDHEVEGGRGDEQYKVLFDKILLEHCR-KHKYLAKTGETFVKLVVRLMERLLD 1183

  Fly  1183 YRSVMQGEENRDKRMTCTVNLLNFYKNEINRKEMYLRYIYKLHNLHLQAENYTEAGFTLKLYASM 1247
            ||::|. :||:|.||:||||:||||| ||.|:|||:||:|||.:||.:.:|||||.:||.|:|.:
Mouse  1184 YRTIMH-DENKDNRMSCTVNVLNFYK-EIEREEMYIRYLYKLCDLHKECDNYTEAAYTLLLHAKL 1246

  Fly  1248 LSWDRETQSFAPFDNSG---QPEWQRKERLYHEILKYFDKGKCWEKGIPLCKELAHLYETRRFDY 1309
            |.|..:..:.......|   ..:.|.||:||.||:.||||||.||:.|.|.||||..|||..|||
Mouse  1247 LKWSEDVCAAHLTQRDGFQATTQGQLKEQLYQEIIHYFDKGKMWEEAIALGKELAEQYETEMFDY 1311

  Fly  1310 NKLSEILIQEAKFFQNILTQLRPEPEYFRVGFYGMGLPLFVRNKQFVYRGLEYERIGAFTQRLQT 1374
            .:|||:|.::|:|::||:..:||:|:||.||:||.|.|.|:|.|.|:|||.||||...|..||.|
Mouse  1312 EQLSELLKKQAQFYENIVKVIRPKPDYFAVGYYGQGFPSFLRGKVFIYRGKEYERREDFEARLLT 1376

  Fly  1375 EFPSAQILGNNSPPDNAILNAPDQYIQISNVRPVGDAQALKTAMVPVPEKIARFYEVNDVTRFIY 1439
            :||:|:.:...|||.:.|..:|.||||...|:|..|..  .....||.|:|..||.||:|.||.|
Mouse  1377 QFPNAEKMKTTSPPGDDIKTSPGQYIQCFTVKPKLDLP--PRFHRPVSEQIVSFYRVNEVQRFEY 1439

  Fly  1440 DRPMYKGTVDKDNEFKSLWIERTILEIASPLPGILRWYEVKQKTMQELTPVEYACEIISNAGKEL 1504
            .||:.||..:.||||.::||||||...|..|||||||:|||...|.|::|:|.|.|.:.....::
Mouse  1440 SRPIRKGEKNPDNEFANMWIERTIYTTAYKLPGILRWFEVKSVFMVEISPLENAIETMQLTNDKI 1504

  Fly  1505 SELIVQYKRDPKRNINPFSMRLQGTIDANVMGGISKYQEAFFSEQFLKS-PQGAGQQANVQRLKV 1568
            |.::.|:..||...|||.||.|.|.:|..||||.:.|::|||::::|:. |:..||   :::||.
Mouse  1505 SSMVQQHLDDPGLPINPLSMLLNGIVDPAVMGGFANYEKAFFTDRYLQEHPEAHGQ---IEKLKD 1566

  Fly  1569 LILEQIQILEQALELHGQLAPSGVQPLHNRLLERFSQLKQSLSGMGRLKRQHSESIVNTPLPPLP 1633
            ||..||..|.:.:.:||......::|.|.|:...|.|||:.:
Mouse  1567 LIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKV----------------------- 1608

  Fly  1634 TEQRVAPATASSSLNANAHSNYVYDLDEIYTRPGDTVRPVDPLSSYQTLSKESLSIPLEDTAAPP 1698
             |::....|..|.|:           |...:||...||.....||.:.||..|:|....|:
Mouse  1609 -EKQYGVRTMPSGLD-----------DRRGSRPRSMVRSFTMPSSSRPLSVASVSSFSSDS---- 1657

  Fly  1699 VPNRPRSQNFIGNGSMDSPEVPPKRQPTLNSPNAPPLPPRGITPDKRASNPLIFNDFGSGDAVGR 1763
            .|:||.|..|.                      ..||.|:                        :
Mouse  1658 TPSRPGSDGFA----------------------LEPLLPK------------------------K 1676

  Fly  1764 RHSQQQQQHGQKYSVVDISYDDPEADQQPHSLPPNFQDGSGGHLSVC---FAPNDFRDSGISTTS 1825
            .||:.|.:         :..|||:.:::....    :..:..|..:.   |.|   .||.:..:.
Mouse  1677 MHSRSQDK---------LDKDDPDKEKKDKKK----EKRNSKHQEIFDKEFKP---ADSSLQQSE 1725

  Fly  1826 SRELNHMNLNNLSEDSSSISTITVQHREHCRISSNGSLDYNAAASMNITQRESSTSSFDVEDFPV 1890
            :..|           |.:||.:..|..:...|:..|:            :|..|.|.........
Mouse  1726 AVIL-----------SETISPLRPQRPKSQVINVIGN------------ERRFSVSPASPSSQQT 1767

  Fly  1891 PPPPIPPKSLAVS------SNGVNFSLDEAHSPHPSTQANTTQLNHIQNPNQNHSTNSHHHLQQQ 1949
            |||..|...|:.|      .||: ..:|.|..|.|                            ..
Mouse  1768 PPPVTPRAKLSFSIQPSLELNGM-MGMDVADVPPP----------------------------LP 1803

  Fly  1950 QNGDGDGYSTLQHPMNGLGLPTLPPPPSAVESPMPASAIASSTSSHQHDGGTFXVTICRCHHPPD 2014
            ..|:...|..|....:.:..||.||||                                   ||.
Mouse  1804 LKGNMADYGNLMENQDMMVSPTSPPPP-----------------------------------PPQ 1833

  Fly  2015 QPHQPHKPSSSTSTSDSTATDTASESTESSSSTHSMTPPPPPP 2057
            :...|..||.                          |||||||
Mouse  1834 RQQPPPLPSK--------------------------TPPPPPP 1850

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spg	NP_733258.4	SH3_DOCK_AB	9..64	CDD:212805	24/54 (44%)
		DOCK_N	67..406	CDD:292790	120/351 (34%)
		C2_Dock-B	414..600	CDD:176077	77/197 (39%)
		DHR2_DOCK	1215..1610	CDD:297424	181/398 (45%)
Dock1	NP_001028592.1	SH3_DOCK1_5_A	13..68	CDD:212984	24/54 (44%)
		DOCK_N	72..416	CDD:292790	122/366 (33%)
		C2_Dock-A	424..619	CDD:176076	77/197 (39%)
		DHR2_DOCK	1214..1612	CDD:297424	182/426 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1613..1723		34/186 (18%)
		Phosphoinositide-binding. /evidence=ECO:0000255	1687..1695		3/7 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1753..1778		7/24 (29%)
		Interaction with NCK2 second and third SH3 domain (minor). /evidence=ECO:0000250	1793..1819		7/53 (13%)
		SH3-binding, interaction with CRK. /evidence=ECO:0000255	1799..1805		2/33 (6%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1801..1865		22/139 (16%)
		Interaction with NCK2 third SH3 domain (major). /evidence=ECO:0000250	1820..1836		8/50 (16%)
		Interaction with NCK2 (minor). /evidence=ECO:0000250	1837..1852		10/40 (25%)
		SH3-binding, interaction with CRK. /evidence=ECO:0000255	1838..1843		2/4 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1997
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54452
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000433
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100636
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2355
SonicParanoid	1	1.000	-	-		X372
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	7.750