DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mIF2 and eif5b

DIOPT Version :9

Sequence 1:	NP_651621.2	Gene:	mIF2 / 43382	FlyBaseID:	FBgn0039588	Length:	696	Species:	Drosophila melanogaster
Sequence 2:	XP_001923573.5	Gene:	eif5b / 561194	ZFINID:	ZDB-GENE-050208-20	Length:	1231	Species:	Danio rerio

Alignment Length:	707	Identity:	187/707 - (26%)
Similarity:	280/707 - (39%)	Gaps:	184/707 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   103 DNIEPAFKLADLKIIQEIAKKLGAKT-----RVVATPEETNADEQKERDVAPRPPAAPELL--QP 160
            ||.|.:.|....|..:|.:.:..:::     |   |.||...|:.|:|....|......::  :.
Zfish   579 DNKESSNKTTANKARKEASSESSSESDSDDDR---TKEERLYDKAKKRIEKRRQENLKNIILDKL 640

  Fly   161 RPPVVTVMGHVDHGKTTLLDSLRGADVAAGEAGGITQHIGAFTVTLE----------NGER---- 211
            |.|||.|:||||.|||.:||.||...|..||||||||.|||..|.|:          |.:|
Zfish   641 RAPVVCVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLDTIVEQTKMVKNFDRDNVK 705

  Fly   212 ---VTFLDTPGHAAFSAMRARGAVATDIIVLVVAAEDGVMAQTREVIQLAKEAQVPIIVALNKID 273
               :..:|||||.:||.:|.||:...||.:|||....|:..||.|.|.|.||.:.|.||||||||
Zfish   706 IPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTLESINLLKEKKCPFIVALNKID 770

  Fly   274 K-------PEANI----EKSKR---------------ELAQMGL--ALEEHGGD----VQVIPIS 306
            :       |:.::    :|.|:               |.||.||  ||.....|    |.::|.|
Zfish   771 RLYDWKKSPDTDVVTTLKKQKKNTKDEFDERAKSVIVEFAQQGLNAALFYENKDPRTFVSLVPTS 835

  Fly   307 ALKGTNL-ELLAEAVSTQATLMGLKADPTGLVEGIVVESKTDPRRGKLSTAIVSRGTLRKGSVLL 370
            |..|..: .|:|..|....|::..:......:...|:|.|..|..|.....|:..|.||:|..::
Zfish   836 AHSGDGMGNLIALLVELTQTMLARRLAHCDELRAQVMEVKALPGMGTTIDVILINGHLREGDTII 900

  Fly   371 ----SGLAHAKVRGLFDHNGQPLSEAPPGTPVEILGWRELPLAGDLILEVETEKKAHAVLKYREH 431
                .|....::|||.         .||            ||           |:.....:|.:|
Zfish   901 VPGVDGPIVTQIRGLL---------LPP------------PL-----------KELRVKNQYEKH 933

  Fly   432 E----SQQEKIESSSDE----------IRKKEEEHQAKYRAEREARRLAGRFRMRGGQRVKQVDE 482
            :    :|..||.....|          ..|::|....:....||.::.....::          |
Zfish   934 KEVCTAQGVKILGKDLEKTLAGLPLLVAHKEDEVPVLRDELVRELKQTLNAIKL----------E 988

  Fly   483 NEGKPRVSVIIKGDVHGSVEAILDVLETYNSNESCRLDIVHYGVGNVTEGDLELAKAF------H 541
            .:|     |.::....||:||:|:.|.|      .::......:|.|.:.|:..|...      :
Zfish   989 EKG-----VYVQASTLGSLEALLEFLRT------SKVPYAGINIGPVHKKDVMKASTMLEHDPQY 1042

  Fly   542 AIIYAFAV----ETPQLKAAKEVNIRSYNIIYRLID-------DLKEQLSSKLPPVEVEEVLGEA 595
            |:|.||.|    ::.::..:..|.|.|..|||.|.|       |.|:|...:...:.|...  :.
Zfish  1043 AVILAFDVKIERDSQEMADSLGVRIFSAEIIYHLFDAFTKYREDYKKQKQDEFKHIAVFPC--KL 1105

  Fly   596 NVLQNFLINEGRKEVPVAGCRCTKGVLKKAQKFRLLRDGEVVYDGPLESMRHLKNEVDTIKKDVE 660
            .:|..|:.|.  ::..|.|.....|||:......:...| .|..|.:.|:......|||.||..|
Zfish  1106 RILPQFIFNS--RDPIVMGVTVEAGVLRTGTPLCVPSKG-FVDIGIVTSIEVNHKSVDTAKKGQE 1167

  Fly   661 -CGLRFRDTKVLP-------------QAGDILQCYTTKM-----------EAQQTDW 692
             |      .|:.|             :|.||:....|:.           |.|::||
Zfish  1168 IC------VKIEPIPGEAPKMFGRHFEAVDIIVSKITRQSIDALKNWFRDEMQKSDW 1218

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mIF2	NP_651621.2	InfB	158..689	CDD:223606	170/642 (26%)
		IF2_eIF5B	163..327	CDD:206674	83/213 (39%)
		IF2_mtIF2_II	336..431	CDD:293903	20/98 (20%)
		IF-2	482..575	CDD:288813	27/109 (25%)
		mtIF2_IVc	593..680	CDD:293893	24/100 (24%)
eif5b	XP_001923573.5	PRK04004	638..1213	CDD:235195	169/638 (26%)
		IF2_eIF5B	643..856	CDD:206674	82/212 (39%)
		aeIF5B_II	866..976	CDD:293904	27/141 (19%)
		IF-2	977..1078	CDD:288813	27/121 (22%)
		IF2_aeIF5B_IV	1103..1197	CDD:293911	24/104 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1124591at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.820