DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mIF2 and Eif5b

DIOPT Version :9

Sequence 1:	NP_651621.2	Gene:	mIF2 / 43382	FlyBaseID:	FBgn0039588	Length:	696	Species:	Drosophila melanogaster
Sequence 2:	NP_938045.2	Gene:	Eif5b / 226982	MGIID:	2441772	Length:	1216	Species:	Mus musculus

Alignment Length:	789	Identity:	196/789 - (24%)
Similarity:	314/789 - (39%)	Gaps:	209/789 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    47 EEKKSPRIIEYSPKKFQTAGGGASDIWR---------------HMTV---------AQLAKELER 87
            |::::|..:|  |::.:.......|.|.               |:.|         .:..:|.|.
Mouse   481 EKEETPPSVE--PEEEEDTEDAGLDDWEAMASDEEREKEGNMIHIEVEENPEEEEEEEEEEEEEE 543

  Fly    88 SLDDVQEAMLYVKGADNIEPAFKLADLKIIQEIAKKLG--AKTRVVATPEETNAD-----EQKER 145
            |.|:.:|.  ..:|:|..|...||:|    ::.:.|.|  ..::..::..|.::|     |::..
Mouse   544 SEDEEEEG--DSEGSDGDEEDCKLSD----EKDSGKAGDTKPSKDASSDSEYDSDDDRTKEERAY 602

  Fly   146 DVAPRPPAAPEL--------LQPRPPVVTVMGHVDHGKTTLLDSLRGADVAAGEAGGITQHIGAF 202
            |.|.|......|        .:.|.|::.|:||||.|||.:||.||...|..||||||||.|||.
Mouse   603 DKAKRRIEKRRLEHGKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGAT 667

  Fly   203 TVTLE----------NGER-------VTFLDTPGHAAFSAMRARGAVATDIIVLVVAAEDGVMAQ 250
            .|.||          |.:|       :..:|||||.:||.:|.||:...||.:|||....|:..|
Mouse   668 NVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQ 732

  Fly   251 TREVIQLAKEAQVPIIVALNKIDK-------PEANI----EKSKR---------------ELAQM 289
            |.|.|.:.|..:.|.||||||||:       |::::    :|.|:               |.||.
Mouse   733 TIESINILKSKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQ 797

  Fly   290 GL--ALEEHGGD----VQVIPISALKGTNL-ELLAEAVSTQATLMGLKADPTGLVEGIVVESKTD 347
            ||  ||.....|    |.::|.||..|..: .|:...|....|::..:......:...|:|.|..
Mouse   798 GLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKAL 862

  Fly   348 PRRGKLSTAIVSRGTLRKGSVLL----SGLAHAKVRGLFDHNGQPLSEAPPGTPVEILGWRELPL 408
            |..|.....|:..|.|::|..::    .|....::|||.         .||  |:     :||.:
Mouse   863 PGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLL---------LPP--PM-----KELRV 911

  Fly   409 AGDLILEVETEKKAHAVLKYREHESQQEK--------IESSSDEIRKKEEE--HQAKYRAEREAR 463
            ..    :.|..|:..|....:......||        :....|||...::|  |:.|
Mouse   912 KN----QYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIHELK-------- 964

  Fly   464 RLAGRFRMRGGQRVKQVD-ENEGKPRVSVIIKGDVHGSVEAILDVLETYNSNESCRLDIVHYG-- 525
                       |.:..:. |.:|     |.::....||:||:|:.|:|        .::.:.|
Mouse   965 -----------QTLNAIKLEEKG-----VYVQASTLGSLEALLEFLKT--------SEVPYAGIN 1005

  Fly   526 VGNVTEGDLELAKAF------HAIIYAFAV----ETPQLKAAKEVNIRSYNIIYRLID------- 573
            :|.|.:.|:..|...      :|:|.||.|    :..::..:..|.|.|..|||.|.|
Mouse  1006 IGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQ 1070

  Fly   574 DLKEQLSSKLPPVEVEEVLGEANVLQNFLINEGRKEVPVAGCRCTKGVLKKAQKFRLLRDGEVVY 638
            |.|:|...:...:.|...  :..:|..::.|.  ::..|.|.....|.:|:.... .:.....|.
Mouse  1071 DYKKQKQEEFKHIAVFPC--KMKILPQYIFNS--RDPIVIGVTVEAGQVKQGTPM-CVPSKNFVD 1130

  Fly   639 DGPLESMRHLKNEVDTIKKDVE-C--------------GLRFRDT-----KVLPQAGDILQCYTT 683
            .|.:.|:.....:||..||..| |              |..|..|     |:..|:.|.|:.: .
Mouse  1131 IGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDW-F 1194

  Fly   684 KMEAQQTDW 692
            :.|.|::||
Mouse  1195 RDEMQKSDW 1203

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mIF2	NP_651621.2	InfB	158..689	CDD:223606	165/634 (26%)
		IF2_eIF5B	163..327	CDD:206674	79/213 (37%)
		IF2_mtIF2_II	336..431	CDD:293903	21/98 (21%)
		IF-2	482..575	CDD:288813	28/111 (25%)
		mtIF2_IVc	593..680	CDD:293893	22/106 (21%)
Eif5b	NP_938045.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..604		24/130 (18%)
		PRK04004	623..1198	CDD:235195	164/632 (26%)
		IF2_eIF5B	628..841	CDD:206674	78/212 (37%)
		G1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	634..641		5/6 (83%)
		G2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	659..663		3/3 (100%)
		G3. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	698..701		2/2 (100%)
		G4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	752..755		2/2 (100%)
		G5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	820..822		1/1 (100%)
		aeIF5B_II	851..961	CDD:293904	27/129 (21%)
		IF-2	962..1063	CDD:288813	29/132 (22%)
		IF2_aeIF5B_IV	1088..1182	CDD:293911	18/98 (18%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0532
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810