DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ALiX and RIM20

DIOPT Version :9

Sequence 1:	NP_651582.1	Gene:	ALiX / 43330	FlyBaseID:	FBgn0086346	Length:	836	Species:	Drosophila melanogaster
Sequence 2:	NP_014918.1	Gene:	RIM20 / 854449	SGDID:	S000005801	Length:	661	Species:	Saccharomyces cerevisiae

Alignment Length:	749	Identity:	203/749 - (27%)
Similarity:	330/749 - (44%)	Gaps:	127/749 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSKFLGVPLKKPSEVDVIKPLNNLIQSTYNGASEEEKGKYGEAVNEFSKQRNTAIWKFFEKYEAS 65
            ||:.|.:|||:..|||....|:.||.:|    |.:....:...:.:....||.||     ..:.|
Yeast     1 MSELLAIPLKRTLEVDFATELSKLIDTT----SFQTASFFQSDILKVVDARNNAI-----APDIS 56

  Fly    66 LEIVYA---YYDQICALETKISVSELQIPFKWKDAFDKGSIFGGKISLTHTSLLYEKVCVLFNIA 127
            ::.:.|   ||..:..||.|...:  ||.|.|.....:.|     ...:..||.:||:.:::||.
Yeast    57 IDGLSALKEYYVILLQLEKKFPNN--QIEFTWFQTLSQKS-----RGTSQYSLQWEKLTIIYNIG 114

  Fly   128 ALQSNIAANQSLDSDDGLKLTIKLLQQSAGIFQYL----KGATPAAVPSEPTPDLSQDTLTVLQA 188
            .:.|.:|.|.:.|:.:.||.:....|.:||.|:::    |..       |..|.:...||..|.:
Yeast   115 CMYSLLALNSNNDAAESLKTSCLYFQNAAGCFKHVLDHQKNL-------ETIPVVDDATLNALTS 172

  Fly   189 LMVAQAQEVFILKAIKDNLKDQIIAKLCCQAEESYADVLRAMQK-ESVRSLWEKEWIPTIAGKQA 252
            ||:|||||.|..||::|..||.:||||..|..:.|.:.:...|: :.:||    :||..:..|:|
Yeast   173 LMLAQAQECFWFKAVQDKHKDSLIAKLSQQIVDFYCEAINDAQRGKLIRS----DWINHLKAKKA 233

  Fly   253 GFHALTQLYQSLVCRAAKKIGEEIARLRNAIDLFKAAQTRSGNETYLDEYFSRAK------RNLT 311
            .|.|:|....:|.....|:.|..:..|:..:...        ||:.|.   |:||      .:|.
Yeast   234 YFSAVTYYRIALSFNEKKQFGNVVKALQMGLQFI--------NESTLS---SQAKFKTVVESSLK 287

  Fly   312 ESTKDNEFIYNEIIPELSTLTSPGKAQLAKPLPIAVPLAENFKD--IFSSLVPVELHRALTASDM 374
            |:.:||||||.:.:|  |.|.|...|.:.||...|..|....||  :|..|:|:|:....||.:.
Yeast   288 EAQRDNEFIYLQEVP--SELPSIKPALMVKPSSSATLLPSIKKDETLFKDLIPIEVMEYCTAYNE 350

  Fly   375 RRNEIVNVEIMKLREATQTLNAVLASLN--LPAAVETADGNSGLPPSLKEKANEVRQKGGIENVQ 437
            |::|.|.          |.:...|||||  |..::.|.....||     .|.:|..    :.:.|
Yeast   351 RQDEYVE----------QRVTNPLASLNKLLKESLTTFQIPQGL-----TKVSEAE----LSHYQ 396

  Fly   438 TMLKDLPELLNRNREI-LDETERLLDEERDSDNQLRAQFKD-RWTRISSDKLTEMFRTNAKKYRE 500
            ..|.:| .:.|:|.:: ||..|::|:||..:|||||.:... .||...|......:....||.|.
Yeast   397 ASLNNL-LINNKNVQVQLDNIEQILNEEAFTDNQLRLKHGTLNWTLPESSTTNTAYYEKLKKLRG 460

  Fly   501 VITNAIEADKVVRQKFEANQKGIQLLSLPPDQIQQSLPSASGSVDPNCSSVQRLKKLMDDVETIK 565
            .:......||...:.|::..|     :|...:|:  ||.::   ||          |.:.::.|.
Yeast   461 YLDEGSAIDKQTNELFQSIDK-----NLIGSEIR--LPESN---DP----------LTNKIKMII 505

  Fly   566 AEREAI--ESELKGATFNMKDEFLIALQKDGAID-EPALSLARIGQV--LNPLQQQVRESVERQQ 625
            .||...  .:..|.:.:.:..:.:.:.:|:|.:| ||..    ||.:  .:...:.|..:.|...
Yeast   506 QERNDYIDRTRRKSSEYRILPKIITSYKKNGTVDFEPIF----IGHLKYFDEDLRYVNSTKEENI 566

  Fly   626 SLVSEIQSAHGAFVSETGSCGSS------------RDTLYQELATAFDSYIELSGNLQEGTKFYN 678
            .|:.|:.      :|:..:.|.|            |:...::|..:|....|:..||..||.||.
Yeast   567 KLIEEVN------LSKKNNPGRSGIEPKKMVRIDPRELYIEDLRYSFKLLDEVKENLSAGTAFYE 625

  Fly   679 DLTQLLVVFQNKISDFVFARKTEKEELLKDLTTE 712
            :|........|::.::..||:.||..|.|.||.|
Yeast   626 NLITSTSNLYNEVQEYDTARRAEKARLDKSLTFE 659

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ALiX	NP_651582.1	BRO1_Alix	2..347	CDD:185763	105/358 (29%)
		V_Alix	362..698	CDD:185748	82/356 (23%)
		ALIX_LYPXL_bnd	415..701	CDD:290660	69/304 (23%)
		MFMR	733..822	CDD:285072
RIM20	NP_014918.1	BRO1_ScRim20-like	3..343	CDD:185764	112/379 (30%)
RIM20	NP_014918.1	V_Alix_like	338..645	CDD:185746	82/356 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346516
Domainoid	1	1.000	123	1.000	Domainoid score	I1220
eggNOG	1	0.900	-	-		E1_KOG2220
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H22614
Inparanoid	1	1.050	172	1.000	Inparanoid score	I999
Isobase	1	0.950	-	0.704428	Normalized mean entropy	S2147
OMA	1	1.010	-	-		QHG53532
OrthoFinder	1	1.000	-	-		FOG0003348
OrthoInspector	1	1.000	-	-		oto99191
orthoMCL	1	0.900	-	-		OOG6_102685
Panther	1	1.100	-	-	LDO	PTHR23030
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R147
SonicParanoid	1	1.000	-	-		X4332
TreeFam	1	0.960	-	-
	16	15.740