DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tusp and tulp4b

DIOPT Version :9

Sequence 1:	NP_001263014.1	Gene:	Tusp / 43317	FlyBaseID:	FBgn0039530	Length:	1433	Species:	Drosophila melanogaster
Sequence 2:	XP_021322965.1	Gene:	tulp4b / 562633	ZFINID:	ZDB-GENE-130530-708	Length:	1649	Species:	Danio rerio

Alignment Length:	1768	Identity:	546/1768 - (30%)
Similarity:	760/1768 - (42%)	Gaps:	493/1768 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 DCTILSLSWMGKVPDDIPEDEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCRKNMDYPLRTN 78
            |..||.|||.|:||....|.   .:.|..||:||||||||.||:||||||:||||::.:.|.|.|
Zfish    27 DSNILCLSWKGRVPKSEKEK---PVCRRRYYEEGWLATGNARGVVGVTFTSSHCRRDRNTPQRIN 88

  Fly    79 YNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMA 143
            :|||||.|:|:||:||||:||||:||..|.|||||:||||||:||:|||...|:.|:|||||..|
Zfish    89 FNLRGHNSEVVLVRWNEPFQKLATCDMEGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQA 153

  Fly   144 LICYQDGFVLVGSVAGQRYWSSMLNLESTITCGIWTPDDQQVYFGTTQGQVIVMDVHGAMVSQVQ 208
            ||.|:|||||||||:|||:|||.:||||.|||||||||||||.|||..|||||||.||.|::.|.
Zfish   154 LIAYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHVL 218

  Fly   209 LSNDVPITSMAWSCEKFKMEEGEEAE-------PGVTNAAKRSFVLAVSFQNGYIYLLKSFDDVS 266
            |.....|.||:|:|..|.:|:..|::       |......|.  :|.|||.:|.|.|:.::||:|
Zfish   219 LHESDGIVSMSWNCPNFLVEDSTESDTDSDDQTPAHVQNLKP--LLTVSFISGDISLMNNYDDLS 281

  Fly   267 PAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTN-LVKFYTETGTCLY 330
            |   |...:|...:.::|.:..:||||||..|.|        :.|.|...| |||||...|..:|
Zfish   282 P---NIIRSGLKDVEVQWCSQGDLLAVAGMERHG--------LPTDSLMRNALVKFYNVQGEHIY 335

  Fly   331 QAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVGSESLLPLLP 395
            ....| :...::.|.|||.|.|||:|.|..:::..|..|||||||||:..|.:::..|..:..|.
Zfish   336 TLETP-AQRPITTICWGHRDSRLFLACGPALYVVRVEHRVASLQLLCQQGIASALKEERDVGKLN 399

  Fly   396 LPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLE 460
            :||.:.|.:...|..||:..:||..::|||||.|...:.||||||.|.:::.. :.|.|||||||
Zfish   400 MPSLLCSYVTTAFIPTIKPPIPDPNNIRDFVSYPTAGNERLHCTMKRSEENPE-AGGPCYTLYLE 463

  Fly   461 YLGGLVPLLKGKRTSKIRPEFVIFDPQVNDSPLYFQYSAEAKSSSGSSQSTTTGNSGRTDSSDSD 525
            :||||||:|||:|.||:||||||.||:::.       .|:....:|.. |..|.:...:||||.:
Zfish   464 HLGGLVPILKGRRISKLRPEFVIMDPKMDG-------KADEVCVNGMI-SYMTDSCNCSDSSDIE 520

  Fly   526 FEER---------SRFG-SPRTPR---KKRVRPKRRHQAGDRLSASGGGVSNDP---DSLDELAY 574
            ..:.         ||.. ||:.||   :.|..||.. |....:|.|.......|   .||....:
Zfish   521 LSDEWVGRKSPKLSRGNRSPKLPRINMESRKSPKLA-QTSQEMSRSPRLPKKPPLRSPSLTRREF 584

  Fly   575 -VDTLPEQEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSLLHLQPRQMTLVITELRD-- 636
             ||...|... |.:||||||||||||.|||..:|||||.|.||||||||||||||:.:.|:|.
Zfish   585 PVDGFSEHNY-LAQVTSNIWGTKFKIVGLASFLPANLGAVIYKTSLLHLQPRQMTIYLPEVRKIS 648

  Fly   637 -DFPPGPDPSFNPNIFSEDEDEHHQHQGIHHDAVPQVNVITAQDGASLKPPI----IPQRRLTDG 696
             ||.  ..|.||||:||||||:           :|    :....|.|...|.    ||
Zfish   649 LDFM--SLPVFNPNVFSEDEDD-----------LP----VMGPSGVSADNPSCTVNIP------- 689

  Fly   697 ASAPLIAP---MSPRPN-----RILARHKNSLTVNGERGSGSSAGLSPLARAESYDDDSSNESQE 753
             .||:.:|   |||..:     .:||.....|.|.....:.::|..:..|.|.:....::..:..
Zfish   690 -IAPIHSPAQAMSPTQSIGLVQSLLANQNIQLDVLTNPTATAAAATAAAAAAAAAATAAAATAAA 753

  Fly   754 AGAA---GSQSTTVLLHQAPSNGSGPGPSCSKSITRPKTISSFKNSYSRSSSNSSCQSRHAISPL 815
            |.|.   .||.|....:..|:..|.||......:.....:|
Zfish   754 ANATVSEHSQDTVTAQYTVPTRYSNPGQVIFSGLEMNNILS------------------------ 794

  Fly   816 YCDGAVP-----TLQSPKNAVAPSDIIFERPAVPAAGQTTLMSYSSNADYANNVVQVKNALMSEP 875
               |.:|     ..|.|...:.||          .:|..||     :..:...:.|:
Zfish   795 ---GTLPPPPHHLPQPPHQPLQPS----------PSGYATL-----SLHHLQLMPQI-------- 833

  Fly   876 VRSANSHVNPVPLNLNLNLERMDARVKCTAPTTSSTAKRREMLYID-EETQSPTPTPSSSSMKRT 939
                 .|.:..|       :|.:..:....|:...:....:.|.|. .:...|.|.|.
Zfish   834 -----PHPDQPP-------DRGEQVLPLKVPSRPQSFIEMDTLEIQLRKVNPPPPYPG------- 879

  Fly   940 PTVVSIAPALPDS------ITRSCSVGYLDSVAITPSDEALSALRKDAPN----KRLILVDKKRN 994
             ||||.|.|:|.:      ::............:|..:.:|..:....|.    :|:...|...|
Zfish   880 -TVVSAAAAIPTTAPPGLLVSNENGTTLSTDTCLTKDEFSLHPISLQYPTPLGYERITTFDSSGN 943

  Fly   995 -----RRKRQQQEDARRHKLQQTGKSKSLDSCDLLSLQTKLSSKEHEQV---------------- 1038
                 |.:|:...:...:.:|..|.|.:|          |::|.|:::|
Zfish   944 VEEVCRPRRRLLRNQNAYGMQGMGSSATL----------KVTSSENKKVQLPYSSATLSRLSVPR 998

  Fly  1039 --------------------VRKLQEISDSSACSS------------------AANTLCFKCRNN 1065
                                :|...:..|||...:                  |..||..|.:.|
Zfish   999 YSIPSGDPPPYPDTSNQMNTIRSPTQRIDSSLIHATLRRDRREPSLKVSQMVDAVRTLPTKSKMN 1063

  Fly  1066 MS---------PSS--ACKRCQPSASSVLDEIT------------AVV----PAVEPAKESPAV- 1102
            .|         ||:  .|.:|..::||....:|            .||    |..:.|:.|..:
Zfish  1064 GSLTLSYQPRIPSALYTCTQCSSNSSSTSVSVTGGGTNSSGIAGGTVVRQDFPPGKGAQHSTIIV 1128

  Fly  1103 ---QAKP-APKKRFDVI--------------TSFTDSPLFTRKHRFGIGRSKETAGTENSTPLLG 1149
               .|.| |.:..::::              ::||:....:::..........|.|..|.|.
Zfish  1129 HSKSASPLASQSSYNLLSPTDNSRDRTVYVNSAFTEDETVSQQCHLEKSMRHLTLGDVNLTV--- 1190

  Fly  1150 RKQDNGFSFVKQLSEVRWRRKE------------------QPAQAQV-----------NGSSNAS 1185
             |:...:.:....:|..|..:|                  ||...::           ..|.|:.
Zfish  1191 -KRPPPYQWDSPAAEQLWIPQEQNLLPGPPPHKPPPFILSQPQHLEIARLPFVLSAKHTPSPNSM 1254

  Fly  1186 T------LERQPQPEITG-------------AACG---TVEATPVEAKAS--------------- 1213
            |      |...|.|.:.|             ..||   .|.::|.....|
Zfish  1255 TLPSPYQLSLSPYPSVVGHGGPPLQPLQSPAQTCGPNDMVASSPFSQPDSTLVLPPGYPSNVSNL 1319

  Fly  1214 -----------------VSLHTQAL----------------TTLENIISRLRDLDEGRLTPPSTP 1245
                             :.||..:|                .||.:  ...:.|::..|:||..|
Zfish  1320 GCCTLPPMYPGTSSCNNLQLHPMSLHPWSTYSTCPPMPDHSATLPS--KTHQTLEKPVLSPPPPP 1382

  Fly  1246 QRLPRSSPASP------------------------------------------------------ 1256
            ...|...|..|
Zfish  1383 PPPPPPPPPPPLPPPPPPVEHLNLQSTSEVVAESGESFQDQSSLNESPQGAERFSKKGRKRLDSR 1447

  Fly  1257 ---------AASKKNKRQQSNSPIRHILNSPLLNRRQRKK-----------QSIIESSDDEGNQT 1301
                     :..|..|..::.|....:::||.|..|::||           :.:..:|::..:.:
Zfish  1448 AEEANMSGVSEGKSKKESRTLSDFNSLISSPRLGSREKKKPKGQKEPLNKTKKLSRTSNEFQDSS 1512

  Fly  1302 NG------SGEESNNAGNG-----KQYRDLETFQKAQLRQKLKRGKIEPNGSASCANPAPVRREF 1355
            ..      ||:|..|....     |..|:|.|   |...:::|..|.......|..:...|   :
Zfish  1513 ESEPELFISGDELMNQSQSSKKGWKSKRNLRT---ANELEEIKCRKANEREDRSLGSQGFV---Y 1571

  Fly  1356 VMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQA 1420
            ||.||.|:|||.:||||||||||||||||||||||..|:|||||||||||||.|||||||||:||
Zfish  1572 VMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELDGRQVMQFGRIDGNAYILDFQYPFSAVQA 1636

  Fly  1421 FAVALANVTQRLK 1433
            |||||||||||||
Zfish  1637 FAVALANVTQRLK 1649

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tusp	NP_001263014.1	WD40	51..>295	CDD:225201	140/250 (56%)
		WD40	81..359	CDD:295369	145/285 (51%)
		WD40 repeat	91..126	CDD:293791	25/34 (74%)
		WD40 repeat	132..168	CDD:293791	25/35 (71%)
		WD40 repeat	173..208	CDD:293791	26/34 (76%)
		WD40 repeat	216..263	CDD:293791	16/53 (30%)
		WD40 repeat	270..333	CDD:293791	21/63 (33%)
		Tub	<588..>644	CDD:279506	39/58 (67%)
		Tub	<1355..1426	CDD:279506	59/70 (84%)
tulp4b	XP_021322965.1	WD40	91..362	CDD:330360	145/284 (51%)
		WD40 repeat	98..136	CDD:293791	27/37 (73%)
		WD40 repeat	142..179	CDD:293791	25/36 (69%)
		WD40 repeat	184..218	CDD:293791	25/33 (76%)
		WD40 repeat	226..265	CDD:293791	10/40 (25%)
		WD40 repeat	285..338	CDD:293791	20/60 (33%)
		Tub	<592..>645	CDD:307359	37/53 (70%)
		Tub	<1543..1642	CDD:307359	65/104 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170596627
Domainoid	1	1.000	133	1.000	Domainoid score	I5020
eggNOG	1	0.900	-	-		E1_KOG2041
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	699	1.000	Inparanoid score	I652
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1445357at2759
OrthoFinder	1	1.000	-	-		FOG0006168
OrthoInspector	1	1.000	-	-		oto39401
orthoMCL	1	0.900	-	-		OOG6_108297
Panther	1	1.100	-	-	O	PTHR16517
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4245
SonicParanoid	1	1.000	-	-		X4453
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.830