Protein Alignment CG5521 and ralgapa2

DIOPT Version :9

• Sequence 1: NP_001163748.1
• Gene: CG5521 / 43242
• FlyBaseID: FBgn0039466
• Length: 1964
• Species: Drosophila melanogaster
• Sequence 2: XP_031757378.1
• Gene: ralgapa2 / 100145357
• XenbaseID: XB-GENE-957487
• Length: 1968
• Species: Xenopus tropicalis
• Alignment Length: 2167
• Identity: 700/2167 - (32%)
• Similarity: 1052/2167 - (48%)
• Gaps: 451/2167 - (20%)

  Fly     1 MFTKKSHADVKKSTAKLQDSKKDSASRLRHLRMILDNVELEESKCLFETNYSHVYFILYDTFIQA 65
            ||:::||.|:||||.|:.|.|||..:||:|||.:|||.|..|.|..||.:|||:|||.|:.||..
 Frog     1 MFSRRSHGDLKKSTQKVLDPKKDVPTRLKHLRAVLDNQESSELKQFFEAHYSHIYFIFYENFITL 65

  Fly    66 EANLKQKELPFHIVHKAHREELDGSLWLLEKILCLLPELLARRWQCHSLSRIMAKLLHLGNSPKL 130
            |::||.|.      :|:.|||||..|:|.||||.||||.:..|||.||:..::.||||.|||.|:
 Frog    66 ESSLKLKG------NKSQREELDSILFLFEKILQLLPERIFYRWQYHSIGSVLKKLLHTGNSIKI 124

  Fly   131 RREGVRYFLLWYQTLGENAPGYVHAMYADLIPGLIVPQKGVVGPDTEFSASDFLTHPNMKADGGM 195
            |.||:|.||||.|.|..|.......::|.|:||....... .||.|    .|.|.:||.....|
 Frog   125 RCEGMRLFLLWLQALQTNCGEEQVLIFACLVPGFPAVMSS-WGPCT----LDKLINPNYSLPDG- 183

  Fly   196 ASVFHDNAFSHPVQSSEVVALLPPSSSEKSAPPDPRDG----LEVLLNSMVHTAACLRWRDNRAQ 256
                       .:...|:..|||..|.||    :|.|.    ||:||..|||.||.|.|| |:..
 Frog   184 -----------KILPEEITPLLPAVSGEK----NPEDQSCFFLEMLLKYMVHQAASLSWR-NKEN 232

  Fly   257 KDHRAFAFLLQRFMDVFLPVFSPNFDVSCSIYNPRLDLPVMR------SINKKEEVMAS------ 309
            :| ..|.||...|...:|....|:|....::|.|.||:|.:|      ::.:..|.:.|
 Frog   233 QD-TGFKFLFNMFRKYYLHHLFPSFTKLTNLYKPVLDIPDVRPKPVYVTLTRNNENIYSTKIQHM 296

  Fly   310 -CVVVLINWVSRFTHERLLSHRLDC----------------TLHIEDV---------------DQ 342
             ..|:.|.|:..|..|:  .:.:.|                ::.:.:|               :|
 Frog   297 AARVLFIKWLVTFFLEK--KYVVSCQSTKNGGGDVLPKLIQSVSVANVNQEKNPELESNSVLAEQ 359

  Fly   343 VRLHGYQQGL----------------------IVRDVLYVNRENINFVHEVYRQAFLLNFTSKP- 384
            .:.|.....|                      :|:.:|...|..:||::||:||||||     |
 Frog   360 EKNHSNSSTLSDRRASNSSLCSIEEDQKSVYEMVQRILLSTRGYVNFINEVFRQAFLL-----PA 419

  Fly   385 -QIEAIRTAIAVYRDWMTGETPPPFLLEP------------------NDDPPPPSNAGGTPRSQR 430
             .:.|.:..:.|||.|:. :..|.|:.||                  ..|....|...|..||..
 Frog   420 CDVSATKKVVKVYRKWIL-QDKPVFMGEPTKPSSAHENFPVMESAVIETDSKQVSEQSGHKRSSS 483

  Fly   431 L-RTPSYVGAIAG----SKDQLVVRAGRQNVLQVFVTNAANVFLVNTANLNICFPTRSRSYRSTP 490
            . ||.|:..|::.    ..:.:.|:||.|..||||:||:|||||     |..|        ...|
 Frog   484 WGRTYSFTSAVSRGCVCEDENMDVQAGDQAALQVFLTNSANVFL-----LEPC--------PEVP 535

  Fly   491 --LEEQTEICKKVLNVYRTMVMNTEMDTRTWEQLLMVLLQVTSIVLHQNQHTLPSGTNKSATLGG 553
              ||||.|.||.||::||.|:|:..||.:||||:|.:||::|..|:.:     |....|......
 Frog   536 LLLEEQVETCKSVLSIYRRMIMDLAMDKKTWEQMLQILLRITEAVVQK-----PKDNLKKDLFSQ 595

  Fly   554 ILGSAIFQTLIVTWIRAHTKVPVNVLLWEKFLNVLQSLTHREELIIEWNKTIQTLTRVFSRYTYG 618
            .|...:|:||||.||||:..|.::..||::.|:||.|||..||||.||:|.:.:||.|.:|..||
 Frog   596 DLAGVLFRTLIVAWIRANLCVYISRDLWDELLSVLSSLTDWEELINEWSKIMDSLTAVLARTVYG 660

  Fly   619 INLLDLPLDRVAESRAEKRRRIGSV----------------WQG-----------SGSNSAANGG 656
            :::..||||:::|.:.:|:|..|..                |:.           |.:.|.|.|.
 Frog   661 LDMSSLPLDKLSEQKEKKQRGKGGFPDPQKGAAVGRSFSLSWRSNPDSHEPMRFRSATTSGAPGV 725

  Fly   657 SAASAAISQNRQRESLAESSSSRSEETSSQV----PLPNHPR-PHHQHTQSQGGTGHGTRPIPLT 716
            ..|...:.|        ::|:.||:..|:.|    .||.... ||..||.|.           |.
 Frog   726 EKARNIVRQ--------KASAKRSQSISNCVHLYEALPAAKSVPHLLHTVSS-----------LL 771

  Fly   717 PMLSRSYSEGSLASAARSSRIRRRRAATPKPKALQPSQLAEN-RINPQDLRRAMSLDSLA----- 775
            |         .::|.:.|.|:.......|:.::......:.| ..:.|:|.|:.|...||
 Frog   772 P---------GISSTSCSQRLSEVDEIQPQDRSAGSESCSGNCEQDAQELARSSSTSDLAEPYPD 827

  Fly   776 ----RKGD------------AEETDSYQEGDNESGA--GSRSPSP-------------------- 802
                :|.|            |:|:....:.|||:..  ..||.||
 Frog   828 LTQGQKIDYSVSESSTDSTFAQESTGRGKSDNEAPTIFVERSSSPVELEWTADSQSSNGRAAYHE 892

  Fly   803 ---TASSGIEGGSIKDAQLQI----------------DASLD-DANSGSYGSGSN----SGSI-- 841
               :.||.|..|.||||::.:                |.::| ||......|.::    :.||
 Frog   893 KGESVSSDIANGYIKDAEIYLMPWQSSEEDHEVNTPTDVAIDHDARQWLQLSPADPTPLTDSIEC 957

  Fly   842 -SGRRCIILGGSAEGWHPDSTSIMWKRMLGALGDVNRIPKADLHAQVFMHLLEMTQNLIKIKQNQ 905
             :|...||.||:..||||||.:::|:|:||.|||||.|..|.:||:||.:|.::...|.||:.|.
 Frog   958 LAGDCSIIAGGNLTGWHPDSAAVLWRRILGILGDVNTIQSAKIHARVFGYLYDLWYKLAKIRDNL 1022

  Fly   906 GISTDNQNTQPMPPLVPPIGIVAPWCYGSLSLDRSFKKGKLWALQLLCSLAIQG---AVNMQQLP 967
            .||.|||::...|.|:||:.:.|.|.:.:.:|...:|:|||.|.:|:||:..:.   ..|...|.
 Frog  1023 SISLDNQSSPAPPVLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICSMMTRRQDVLPNSDFLV 1087

  Fly   968 LFYHALHQLLTGEDRDLIYAILKHLEGPRLLSLLLPGHTLLLLDLVHASAILLTS--LEVSRSTP 1030
            .||..:|..|..:|:|::.|:::|... |...|.|||.|:||.|.:.|:|.:|::  ||    .|
 Frog  1088 HFYLIMHTGLNNQDQDILNAVIRHCPS-RFFFLGLPGFTMLLGDFITAAARVLSTDMLE----AP 1147

  Fly  1031 RAEVAALLGSLLCYPSSLLTRSVLQPTPQKFE-LMECSDLQDHILNIVLRCARREPSAKARCIAL 1094
            |.|...:||||:|:|:......:|..:..:.: ..|..|::.::::.:|:.|:.||:..|||||:
 Frog  1148 RLEAQTILGSLVCFPNVYKDLPLLGSSAGETDTTTEHKDIKSYLIDKLLKNAKDEPAEIARCIAI 1212

  Fly  1095 SQLGQWLLMRLSQPLPASNSGRANLFQQAVPHHKDVHPKASSVYNPRIKEVLQVLLQALQFKHHT 1159
            ..||.|:...|.|                            :..:|:||:.:.|:...|:|.:.|
 Frog  1213 CSLGLWICEELVQ----------------------------NTNHPQIKDAINVIGVTLKFPNKT 1249

  Fly  1160 IAIVAVDSLKLCAERGRQLAAIE-----RVPQLIITAICKAL---EIQSVTKPKDSDKVVLTSLM 1216
            :|.:|.|.|:|.....|:|...:     ::.::::..|...|   |..||    :.||.::.||:
 Frog  1250 VAQMACDVLQLLVSYWRELQNTDLPLSRKITEILVATISFLLPSAENSSV----EMDKKLIVSLL 1310

  Fly  1217 LCLGEFCMAIPAPIMLTPF--------NEQGDTLVLQVLRVLLQVASGAPRHERVKLTADDDFDM 1273
            |||.::|||:|..|:|.|.        :....:|:..:.|||.....|:..:     |....:.:
 Frog  1311 LCLLDWCMALPLHILLEPISTFAIDESHSPKASLLDYIYRVLHCCVCGSNTY-----TQQSQYIL 1370

  Fly  1274 HIAHDDLQG---DGRLPEATYQTSETIQ-------------------KCITAIKLCAKAVSMHLV 1316
            .:|  ||..   |..||....:.||..|                   :.:..|.|.|:.|..|||
 Frog  1371 SLA--DLSSMDYDPFLPLGNVKNSEPQQFFSSVELGDLLTVVEEKKRRNLELIPLTARMVMTHLV 1433

  Fly  1317 THIGHFPMGIGASRLSSMVEEQDDIGNAAYGGQVETRRDSVELPS-VVSAQNMQLFMLNSGLVAS 1380
            .|:||:|:..|.:.|.|::.|..|...|          ::.||.: |..:.|:||.:.|...:.|
 Frog  1434 NHLGHYPLSGGPAVLHSLINENQDNPYA----------EASELSNEVFRSPNLQLCVFNDSALIS 1488

  Fly  1381 FIELPTLKLPG---GGITAGLVTADKQVRVLMRDLNGKACWDASILYSEPRNAEEPPKTTPKIQH 1442
            ::::|.....|   .|:..       .||:::||::||..||:.|:|. |.......|...|:|:
 Frog  1489 YLQIPAESSVGRDPSGVPG-------DVRIIVRDISGKYSWDSGIMYG-PLECHTIFKMEQKVQN 1545

  Fly  1443 SQPLDSMATSMVTHTPSPRHTLRHRPAGVLPLAKDMAPDLDQLDDMLAYIGHTSPECVAPTVSQL 1507
                       |:..|...:..:      ..|......||  ||.:|..:|..||||:....|:|
 Frog  1546 -----------VSQDPVEPYECK------TGLTDGEGEDL--LDTLLEKLGTVSPECLLHPDSKL 1591

  Fly  1508 NAPTASPLSGN--QEAQAISVILNQRLLEQEFVTHSTQAPSPALRHASSNSSLQQPDQRSLHSTT 1570
            |.|::.|...|  ||.:.|..|..|...|.:.|......|...:      :..::|      ||
 Frog  1592 NEPSSPPYGMNREQEEEIIQAINRQSEHEAQHVQMCNSDPGVKV------ACQEEP------ST- 1643

  Fly  1571 ASFDSLPTRTEMPFQYCRLLFSHLGLAGWERRSRTHLLQRSEKLMRELRNVDLQKCRETHKMAVI 1635
                   |:.:.||.:||||.:.||:..||||...|||:::.||:|||:|:|.::||||||:||.
 Frog  1644 -------TQPQAPFYFCRLLLNDLGMNSWERRKNFHLLKKNAKLLRELKNLDSRRCRETHKIAVF 1701

  Fly  1636 YVAAGQEDKGSILRNTSGSSTYEMFVSALGWEIDLETHNGFLGGLPRQG-CGATAPYYATPFLEV 1699
            |:|.|||||.|||.|::||..||.|:..||||:||.||.||:|||.|.| .|.|||||||..:||
 Frog  1702 YIAEGQEDKRSILSNSAGSKAYEDFLGGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEV 1766

  Fly  1700 VYHVATRMPSDSSEAMLLKTRHLGNDEVHIVWSEHNRDYRRDILPTEFCDVLIVVYPLRNGLFRV 1764
            ::||:||:.|||.:::..|.||||||||.||||||.|:||:.|:||:|.|||||:||::|.:|.:
 Frog  1767 IFHVSTRILSDSDDSVTKKLRHLGNDEVQIVWSEHTRNYRKGIIPTDFGDVLIVIYPMKNHMFFI 1831

  Fly  1765 TVNRKPEVPWFGPLANESVVSGACLATLIRATAINASRTKRAALPLYQQFYEERNRSLDSVSSRY 1829
            .:.:||:||:||||.:.::|:...|.||:.||.|||||..::.|||||.|||||...|:::...:
 Frog  1832 EIMKKPQVPFFGPLFDGAIVTEKLLPTLVCATCINASRAVKSRLPLYQSFYEERALYLEAIIHNF 1896

  Fly  1830 KESTTFEDFASRIYNPMPLSTLGTLRESNASSSAAPLASALLDHNRASVKGWVQASIDSGPIMGI 1894
            :|..||||||:::::|.|..:.|.|  ...:|::|.||.|             :|:..|.|.:..
 Frog  1897 REVMTFEDFAAQVFSPSPAYSPGGL--GLPASTSADLAGA-------------EAAEPSSPTLSR 1946

  Fly  1895 APSASAGSTAAMEAATGMSSAS 1916
            | :.|..|.....:|:.:|.:|
 Frog  1947 A-TKSKVSGKLRRSASAISKSS 1967

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG5521	NP_001163748.1	Rap_GAP	1648..1820	CDD:280332	99/172 (58%)
ralgapa2	XP_031757378.1	Rap_GAP	1714..1886	CDD:396632	98/171 (57%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	771	1.000	Domainoid score	I84
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1019	1.000	Inparanoid score	I285
OMA	1	1.010	-	-		QHG47016
OrthoDB	1	1.010	-	-		D157372at2759
OrthoFinder	1	1.000	-	-		FOG0003646
OrthoInspector	1	1.000	-	-		otm48612
Panther	1	1.100	-	-	LDO	PTHR10063
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2517
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.080