Protein Alignment Spag1 and BBS4

DIOPT Version :10

• Sequence 1: NP_651514.1
• Gene: Spag1 / 43239
• FlyBaseID: FBgn0039463
• Length: 469
• Species: Drosophila melanogaster
• Sequence 2: NP_149017.2
• Gene: BBS4 / 585
• HGNCID: 969
• Length: 519
• Species: Homo sapiens
• Alignment Length: 525
• Identity: 99/525 - (18%)
• Similarity: 170/525 - (32%)
• Gaps: 171/525 - (32%)

  Fly     2 EKEKKKS-----LLERYN--IPIHYLDFAHVEKCTNAREMEKIVQILRSGEEGHYPDLQRCAEEK 59
            :|.::|.     :||:.|  |.:||:          .::.|....:::.    ...:.|...|
Human    19 QKPRQKKAPEFPILEKQNWLIHLHYI----------RKDYEACKAVIKE----QLQETQGLCE-- 67

  Fly    60 LKALKPDSKLFRYEEQIKQSTDLDKT--ELKPILDWTDAIKTKDNALNELKKVKQNLNLPSVRKL 122
             .|:...:.:||.|..|::|.:|.:|  .|.|        ::.||    ||:|.::|.|....|
Human    68 -YAIYVQALIFRLEGNIQESLELFQTCAVLSP--------QSADN----LKQVARSLFLLGKHK- 118

  Fly   123 SKIDLEKESKTEKPKPAPKATSPSNTKNKEARIKSTDYRKWDKYDPDEEILRMDLNEERDQEQRE 187
                                 :.....|:.|::...|   |:
Human   119 ---------------------AAIEVYNEAAKLNQKD---WE----------------------- 136

  Fly   188 KIISNHSKSVTTDKLQSERDSLYERLQAQLKNLSQLEKE-----QFAERHRLRGNESFKAKEYEN 247
               .:|:..|....|:.     :.:.|.||.|...|.:.     ...:.|.|.|       :.:.
Human   137 ---ISHNLGVCYIYLKQ-----FNKAQDQLHNALNLNRHDLTYIMLGKIHLLEG-------DLDK 186

  Fly   248 AIEEYNCSIIYDPENAVHAYNNRAVAHLKLKKYFSAISDCQACLQIDPMNIKAHLRMAEAHNAEG 312
            |||.|..::.:.||| ........:.:|:|..|..|.......|..||.|.||.|.........|
Human   187 AIEVYKKAVEFSPEN-TELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHG 250

  Fly   313 KHLESLNVYKKLLDFEPD-----NAIA------KKAVEKLTSM----------------LGEV-- 348
            ....:|..|:.:....|:     |.|.      ||.|..::.:                ||.|
Human   251 DFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHL 315

  Fly   349 ------------------APSSATRLIIEEIDPPQLKTSEPKKEAEKSEPTVVKKPEPVVS---- 391
                              .|......::..:....|:..|..|.| .:|...:.|..|:|:
Human   316 TMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNLEDIENAKRA-YAEAVHLDKCNPLVNLNYA 379

  Fly   392 ------AKKPPPIKDYDLAE----LVKPNRMVK--SNLVSAAEALGNKMQASKGGAPKKPQSPPM 444
                  .:|...:..|...|    |:|.|..::  |.:|..|:.||..:|..:.....||...|.
Human   380 VLLYNQGEKKNALAQYQEMEKKVSLLKDNSSLEFDSEMVEMAQKLGAALQVGEALVWTKPVKDPK 444

  Fly   445 TPKET 449
            :..:|
Human   445 SKHQT 449

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Spag1	NP_651514.1	NlpI	<229..373	CDD:443815	36/190 (19%)
		TPR repeat	229..257	CDD:276809	7/27 (26%)
		TPR repeat	262..293	CDD:276809	6/30 (20%)
		TPR repeat	298..326	CDD:276809	6/27 (22%)
BBS4	NP_149017.2	Required for localization to centrosomes	1..66		10/60 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..25		1/5 (20%)
		TPR repeat	36..61	CDD:276809	5/38 (13%)
		TPR 1	67..100		11/43 (26%)
		TPR repeat	68..95	CDD:276809	8/26 (31%)
		LapB	71..332	CDD:442196	62/336 (18%)
		Interaction with PCM1. /evidence=ECO:0000269|PubMed:15107855	101..337		54/303 (18%)
		TPR repeat	101..129	CDD:276809	9/53 (17%)
		TPR repeat	134..164	CDD:276809	9/63 (14%)
		TPR repeat	169..196	CDD:276809	7/33 (21%)
		TPR repeat	202..229	CDD:276809	4/26 (15%)
		TPR repeat	236..264	CDD:276809	6/27 (22%)
		Spy	250..>446	CDD:443119	36/196 (18%)
		TPR repeat	270..298	CDD:276809	5/27 (19%)
		TPR repeat	303..333	CDD:276809	3/29 (10%)
		Required for localization to centrosomes	338..519		24/113 (21%)
		TPR repeat	338..365	CDD:276809	5/27 (19%)
		TPR repeat	372..397	CDD:276809	4/24 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	440..519		2/10 (20%)