Protein Alignment Nf1 and IRA1

DIOPT Version :9

• Sequence 1: NP_733132.2
• Gene: Nf1 / 43149
• FlyBaseID: FBgn0015269
• Length: 2802
• Species: Drosophila melanogaster
• Sequence 2: NP_009698.3
• Gene: IRA1 / 852437
• SGDID: S000000344
• Length: 3092
• Species: Saccharomyces cerevisiae
• Alignment Length: 2709
• Identity: 538/2709 - (19%)
• Similarity: 995/2709 - (36%)
• Gaps: 682/2709 - (25%)

  Fly    95 TNQTKDTARFEEAMNVKLLLREISQFVDVQS-DSNPNAAQLKALASKVLFALSQNHFSAVFN-RI 157
            |..|.:......:.|:..:.:.|....|:.| :|:..:..|....|..|:..||:..:::.| .:
Yeast   473 TMNTNNNNNNNNSANLNNIPQRIFSLDDISSFNSSRKSLNLDDSNSLFLWDTSQHSNASMTNTNM 537

  Fly   158 SARIQELTSCSEEN------------PDYNDIELIQHIDMDMIKLTKLLQETITKFRSKRAPPLI 210
            .|.:....|.::::            .:|...||..|             ..|.:|       |:
Yeast   538 HAGVNNSQSQNDQSSLNYMENIMELYSNYTGSELSSH-------------TAILRF-------LV 582

  Fly   211 LLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKSKTLVWPLQMLLLI 275
            :|..|:..:          :.::...:.|.||   ||:|:.      ..|.|.|..|....
Yeast   583 VLTLLDSEV----------YDEMNSNSYRKIS---EPIMNI------NPKDSNTSSWGSAS---- 624

  Fly   276 LNPSC------LEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKRGL--GQH 332
            .|||.      |:.:.  |||..|...|                       |:..:.|.|  ||.
Yeast   625 KNPSIRHLTHGLKKLT--LQQGRKRNVK-----------------------FLTYLIRNLNGGQF 664

  Fly   333 SPSKQVTESAAIACVK----LCKASTYINNTDSN--NVVFKLVQFFINDLKALLFNPAKPFSRGQ 391
                 |::.:.|..::    |...::.|:..|||  :|:|.  :.|.|.|       .:....|.
Yeast   665 -----VSDVSLIDSIRSILFLMTMTSSISQIDSNIASVIFS--KRFYNLL-------GQNLEVGT 715

  Fly   392 GYNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNP 456
            .:|.|.....|.   .|...||.....|::....|..|      :.|.|.|.|:.::..|.:
Yeast   716 NWNSATANTFIS---HCVERNPLTHRRLQLEFFASGLQ------LDSDLFLRHLQLEKELNH--- 768

  Fly   457 FRIVNQPRLSWWPQ---------------TDVVHYRSAELRALF---TDTLNKATQGYIAHTPLR 503
               ::.|::|.:.:               .|:....|:.|:.||   .|.|.|||. |......:
Yeast   769 ---IDLPKISLYTEGFRVFFHLVSTKKLHEDIAEKTSSVLKRLFCIIADILLKATP-YFDDNVTK 829

  Fly   504 YITSL-------------TLKSKD--TQKGLTRAEEGPA---HKMLLLLLVRLIHADPTLLLNTQ 550
            .|.|:             ||.:.|  :....|.....|.   |......||..:...| |.:|:.
Yeast   830 IIASILDGHILDQFDAARTLSNDDHVSFDAATSVYTEPTEIIHNSSDASLVSSLSQSP-LSINSG 893

  Fly   551 GKVAH----EVQSSTLELIN----GLVSLVHQTTMPDVAQEAMEALLALH--------------- 592
            ..:.:    ::||....|.|    ..:||.:..|.|..||:...|.|..|
Yeast   894 SNITNTRTWDIQSILPTLSNRSSASDLSLSNILTNPLEAQQNNNANLLAHRLSGVPTTKRYASPN 958

  Fly   593 --------------------APEKIEVWNPEAPINTFWDVS------------------------ 613
                                .|..:.|.:..|..::...::
Yeast   959 DSERSRQSPYSSPPQLQQSDLPSPLSVLSSSAGFSSNHSITATPTILKNIKSPKPNKTKKIADDK 1023

  Fly   614 -------SQV--------------LFSISQKLIQHQI---AN------YTDVLKWLREILICRNT 648
                   |:|              :|||.:::....|   ||      :||::|.|...::..|.
Yeast  1024 QLKQPSYSRVILSDNDEARKIMMNIFSIFKRMTNWFIRPDANTEFPKTFTDIIKPLFVSILDSNQ 1088

  Fly   649 FLQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSD--- 710
            .||             :..:|.|::.:.:......:|.|.....|:...|||..|....:|.
Yeast  1089 RLQ-------------VTARAFIEIPLSYIATFEDIDNDLDPRVLNDHYLLCTYAVTLFASSLFD 1140

  Fly   711 --------ELTVGFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLSRLTLQKRIMTLLRKIE 767
                    |:.:..|:....:...|:.|:            ..|..|.:.:|.::..:.||....
Yeast  1141 LKLENAKREMLLDIIVKFQRVRSYLSNLA------------EKHNLVQAIITTERLTLPLLVGAV 1193

  Fly   768 --------HCVHGVQPAWEETFRNWEVSS----KVLQTYPKCKGEDGQAEVFHRGMGKRRASHQ- 819
                    :|..|..|      |..::|.    :.|:.|.|..|...|..:::.......|...
Yeast  1194 GSGIFISLYCSRGNTP------RLIKISCCEFLRSLRFYQKYVGALDQYSIYNIDFIDAMAQDNF 1252

  Fly   820 --SSEHDLEEQINEWANMTWFLLALGGVCL------HKR----SSSRQMLLQQSQNNASL-GSLA 871
              |....|:.::..  |:..::.....:.|      :|:    |.|:.:..::..:..|| |.||
Yeast  1253 TASGSVALQRRLRN--NILTYIKGSDSILLDSMDVIYKKWFYFSCSKSVTQEELVDFRSLAGILA 1315

  Fly   872 ---------QNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEK 927
                     |....|.|.....|    .::|..:..||           ..|.:.|:|.:  .:|
Yeast  1316 SMSGILSDMQELEKSKSAPDNEG----DSLSFESRNPA-----------YEVHKSLKLEL--TKK 1363

  Fly   928 IGLNIQK-----NVKELVGEE-----MSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDINTQFI 982
            :...|.|     |...|:..|     :|.:|:|:.|:.:...:....|:...::::.:..::.|:
Yeast  1364 MNFFISKQCQWLNNPNLLTRENSRDILSIELHPLSFNLLFNNLGLKIDELMSIDLSKSHEDSSFV 1428

  Fly   983 --EHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMML----GIVRYVRHLDMTVYAIRIK 1041
              |..|.|:::||  |.:.|                 |.:||    .::..|..|...|..|.||
Yeast  1429 LLEQIIIIIRTIL--KRDDD-----------------EKIMLLFSTDLLDAVDKLIEIVEKISIK 1474

  Fly  1042 T--------KLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQ------IAPPSSAD 1092
            :        ::.::.........:|........:||.::.:..|...:.::      ::.|....
Yeast  1475 SSKYYKGIIQMSKMFRAFEHSEKNLGISNHFHLKNKWLKLVIGWFKLSINKDYDFENLSRPLREM 1539

  Fly  1093 AAILTNTSLIFRDLDQACMEAVAALLRGLPLQ-PEESDRGDLMDAKSALFLKYFTLFMNLLNDCI 1156
            .....:...::.|......:|:|.|...:||: |..|.:.|...:.:..|..:||:.:..|
Yeast  1540 DLQKRDEDFLYIDTSIESAKALAYLTHNVPLEIPPSSSKEDWNRSSTVSFGNHFTILLKGL---- 1600

  Fly  1157 DSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRA 1221
               |...::|..|:      ....|::.|....|:|::||..||::..|..::.:||:|:...|.
Yeast  1601 ---EKSADLNQFPV------SLRHKISILNENVIIALTNLSNANVNVSLKFTLPMGYSPNKDIRI 1656

  Fly  1222 AFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARV 1286
            ||:.|...|:   |.:....|....|:...:...:..|.....|....:||  .:.:.:|..|..
Yeast  1657 AFLRVFIDIV---TNYPVNPEKHEMDKMLAIDDFLKYIIKNPILAFFGSLA--CSPADVDLYAGG 1716

  Fly  1287 LVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRP 1351
            .:..||.::....|:..:..:|::.:.....:.|.||..::.::...:..|..||...|.|:::.
Yeast  1717 FLNAFDTRNASHILVTELLKQEIKRAARSDDILRRNSCATRALSLYTRSRGNKYLIKTLRPVLQG 1781

  Fly  1352 LLDEEEETCFEVDPARLDP-TEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLS 1415
            ::|.:|.  ||:|  ::.| :|:.|:..:.......::.|||.:|.|.||.:|..:|..:|...|
Yeast  1782 IVDNKES--FEID--KMKPGSENSEKMLDLFEKYMTRLIDAITSSIDDFPIELVDICKTIYNAAS 1842

  Fly  1416 KRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVE-- 1478
            ..||.....   |||:.:|||||.||:|||....|: ...|:..::..:.::|::|::||..|
Yeast  1843 VNFPEYAYI---AVGSFVFLRFIGPALVSPDSENII-IVTHAHDRKPFITLAKVIQSLANGRENI 1903

  Fly  1479 ------FSKEQHMLCFND----FLRDHFE-AGRRFFIQIASDCETVDQTSHSMSFISDANVLALH 1532
                  .|||:.:...:|    ||.:..: ....|.:.:..|...:   |...||        ||
Yeast  1904 FKKDILVSKEEFLKTCSDKIFNFLSELCKIPTNNFTVNVREDPTPI---SFDYSF--------LH 1957

  Fly  1533 RLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMAT-----LLAYLGPP------EHKP--VDSHMMF 1584
            :..:.::..|...:.:  :.|..|...|.|...     :|..||.|      |..|  |::...:
Yeast  1958 KFFYLNEFTIRKEIIN--ESKLPGEFSFLKNTVMLNDKILGVLGQPSMEIKNEIPPFVVENREKY 2020

  Fly  1585 SSYARWSSIDMSSTNFEEIMVKHQMHEKEEF----KTLKSMNIFYQAGTSKSGYPVFYYIARRYK 1645
            .|...:    ||...|:::.:|.:..:...|    .||..:.|.....|:
Yeast  2021 PSLYEF----MSRYAFKKVDMKEEEEDNAPFVHEAMTLDGIQIIVVTFTN--------------- 2066

  Fly  1646 IGETNG---DLLIYHVI-LTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVH 1706
             .|.|.   |.|:|.|: :..:.:| |...||||.|..........:....:|.::|..|..|..
Yeast  2067 -CEYNNFVMDSLVYKVLQIYARMWC-SKHYVVIDCTTFYGGKANFQKLTTLFFSLIPEQASSNCM 2129

  Fly  1707 AVYIYNCN-----SWVREYTKFHDRILAPLKGNRKLLFLESPNKLTD----FIDAEQQK------ 1756
            ..|.:|.|     .|...||                  :|:|..:|.    ||::...:
Yeast  2130 GCYYFNVNKSFMDQWASSYT------------------VENPYLVTTIPRCFINSNTDQSLIKSL 2176

  Fly  1757 -LPGATLSLDEDLKVFSNALKLSHKDTK----VAIKVG----------PTALQITSAEKTKVLAH 1806
             |.|.:|.:.:|::|..:.:.|..|:.|    |::|:|          |...::|.:.:|    .
Yeast  2177 GLSGRSLEVLKDVRVTLHDITLYDKEKKKFCPVSLKIGNKYFQVLHEIPQLYKVTVSNRT----F 2237

  Fly  1807 SVLLNDVYYASEIEEVCLVD----DNQFTLSITNESGQLSFIHNDCDNIVQAIIHIRNRWE---- 1863
            |:..|:||..|.:..|.:.:    .::||||:.||. :|.|.......||:...:.:.:.|
Yeast  2238 SIKFNNVYKISNLISVDVSNTTGVSSEFTLSLDNEE-KLVFCSPKYLEIVKMFYYAQLKMEEDFG 2301

  Fly  1864 --------LSQPDSVTVHQKIRPKDVPGTLLN----MALLNLGSCDPNLRTAAYNLLCALTATFD 1916
                    .|...|.........|:| |.:::    :.|:.|.:.|..::..:||||.|....|:
Yeast  2302 TDFSNDISFSTSSSAVNASYCNVKEV-GEIISHLSLVILVGLFNEDDLVKNISYNLLVATQEAFN 2365

  Fly  1917 LKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHL----C- 1976
            |....:|.::....:|.:.|.|:..:.:..:.:...||....:..:.|.:...|..:|:    |
Yeast  2366 LDFGTRLHKSPETYVPDDTTTFLALIFKAFSESSTELTPYIWKYMLDGLENDVIPQEHIPTVVCS 2430

  Fly  1977 LEYMTPWLKNLVKFC-KSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDM 2040
            |.|   |:.||.:.. .:||:.....:|:|:..||.||:.:.....:...:||         ..:
Yeast  2431 LSY---WVPNLYEHVYLANDEEGPEAISRIIYSLIRLTVKEPNFTTAYLQQIW---------FLL 2483

  Fly  2041 VLDNFLHKSITYGLGSPQVEIMADTAVALASAN------VQLVSKKVITRI-CRVMDKSCTNPTQ 2098
            .||..|...|        ||.:...|:...|.|      |.:::....|.| |:|::|.......
Yeast  2484 ALDGRLTNVI--------VEEIVSHALDRDSENRDWMKAVSILTSFPTTEIACQVIEKLINMIKS 2540

  Fly  2099 YLEQHMM------WDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLV 2157
            :|....:      |.::.||.:..:.:.|.:.|.....:|.:...::.|:..|...:|.|.:.|:
Yeast  2541 FLPSLAVEASAHSWSELTILSKISVSIFFESPLLSQMYLPEILFAVSLLIDVGPSEIRVSLYELL 2605

  Fly  2158 INIIHSLCTCTNPSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLG 2222
            :|:.|||  ..|.|..|..::.|.:....|:..|...:.|.|:.|...:..|.:|
Yeast  2606 MNVCHSL--TNNESLPERNRKNLDIVCATFARQKLNFISGFSQEKGRVLPNFAAS---------- 2658

  Fly  2223 NERVTQPLPADRERLSLPS----LEVITDALLEIMEACMRDVPD-CEWLNTWTSLARSFAFCYNP 2282
                           |..|    |::.|..::.:||  ...:.: .:|...:........|.:..
Yeast  2659 ---------------SFSSKFGTLDLFTKNIMLLME--YGSISEGAQWEAKYKKYLMDAIFGHRS 2706

  Fly  2283 ALQPRALIVYGCISKSVTD-HEVKQLL----RILVKALESFNDLILIEALVMCLTRIQPLLRPES 2342
            ....||:::.|.:|||.|. ...|:||    ::..:.:.....:.:|.|.|...::|...|.|.|
Yeast  2707 FFSARAMMILGIMSKSHTSLFLCKELLVETMKVFAEPVVDDEQMFIIIAHVFTYSKIVEGLDPSS 2771

  Fly  2343 PIHRALFWVAISVLQLDEITLYGAGLALLE---QNLHTLKSQGCFDKKETIAEVMMKTRE----- 2399
            .:.:.|||:|...::.....|:..||..:.   :.|:|:..|..||.| ::|:.:|::|.
Yeast  2772 ELMKELFWLATICVESPHPLLFEGGLLFMVNCLKRLYTVHLQLGFDGK-SLAKKLMESRNFAATL 2835

  Fly  2400 --KLE------WHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHP 2435
              |||      |:            ..||...::|.:..|...|
Yeast  2836 LAKLESYNGCIWN------------EDNFPHIILGFIANGLSIP 2867

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	83/357 (23%)
		CRAL_TRIO_2	1633..1767	CDD:404584	31/153 (20%)
		PH_NF1	1759..1868	CDD:270123	31/138 (22%)
		MOR2-PAG1_mid	<1931..>2109	CDD:372971	41/196 (21%)
IRA1	NP_009698.3	RasGAP_Neurofibromin_like	1679..1995	CDD:213341	78/338 (23%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	106	1.000	Domainoid score	I1446
eggNOG	1	0.900	-	-		E1_KOG1826
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0005399
OrthoInspector	1	1.000	-	-		otm46912
orthoMCL	1	0.900	-	-		OOG6_103500
Panther	1	1.100	-	-	O	PTHR10194
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R472
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	9	8.840