Protein Alignment Nf1 and RasGAP1

DIOPT Version :10

• Sequence 1: NP_733132.2
• Gene: Nf1 / 43149
• FlyBaseID: FBgn0015269
• Length: 2802
• Species: Drosophila melanogaster
• Sequence 2: NP_001261664.1
• Gene: RasGAP1 / 39158
• FlyBaseID: FBgn0004390
• Length: 1181
• Species: Drosophila melanogaster
• Alignment Length: 1023
• Identity: 208/1023 - (20%)
• Similarity: 364/1023 - (35%)
• Gaps: 280/1023 - (27%)

  Fly   960 VEKFFDQQGQVNVNVTDINTQ----------FIEHTIYIMKS-----------ILDPKANKDPNN 1003
            |::..:.||.|||.|.....|          ..:||:...::           :.|.|.|.:.:|
  Fly   153 VDQDSEVQGMVNVEVAFTEAQQTQSLSEGIDLGQHTLRHHQNLPHHSHQQRAHLNDYKENSELSN 217

  Fly  1004 DQ----PSPSEHLGVTSIEGMMLGIVRYVRH----------LDMT---------------VYAIR 1039
            .|    .:.|......:::....|:..:|.|          ::.|               ...:.
  Fly   218 IQRASAAAASSSSAAMTLKTRAAGLFGHVHHPPSQTQHFPIINTTSTSSDQLSNWKSHGRFVGVT 282

  Fly  1040 IKTKLCQLVEVMMKRRDDLAFRQ---------------------EMKFRNKLVEYLTDWVMGTSH 1083
            ||...|.          |||.:|                     ..|.|.|.|:...|..|....
  Fly   283 IKVPACV----------DLAKKQGTCDPFVVCTAHYSNKHQVTRRTKQRKKTVDPEFDEAMYFDL 337

  Fly  1084 QIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLM--------DAKSAL 1140
            .|    .|||. .|||:    ..:::.....::..:|..:.|...  .||:        ||..|:
  Fly   338 HI----DADAG-STNTT----GSNKSAGSLESSANKGYSIYPVGG--ADLVEIVVSVWHDAHGAM 391

  Fly  1141 FLKYF--TLFMNLLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGA--NI 1201
            ..|.|  .:.:.:||     .:.::.:|.:.....:||.......:| |||..:.:...|.  ::
  Fly   392 SDKVFLGEVRLPMLN-----KQEQQAVNPSAWYYLQPRSMTHSSRSL-NATPRSCATPPGTRLSV 450

  Fly  1202 DS--GLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGE 1264
            ||  |.:. ::|.|..|            .:....|..|.:  .:|.:..:|....|:.:|..||
  Fly   451 DSTIGSLR-LNLNYTAD------------HVFPLATYDDLM--NLLLESVDQRPITVSAVSILGE 500

  Fly  1265 LPIAMALANVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIM 1329
            |           .|...|:|:.||.||.....::|::..:...|:.......|:||||:|.||:|
  Fly   501 L-----------VSGKTEVAQPLVRLFTHTERIAPIIKALADHEISHLTDPTTIFRGNTLVSKMM 554

  Fly  1330 AFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIIN 1394
            ....::.|..||...|.|::..:: .|::.| |:||:::.....::.:.:||....::||:||..
  Fly   555 DEAMRLSGLHYLHQTLRPVLSQIV-AEKKPC-EIDPSKIKDRSAVDTNLHNLQDYVERVFEAITK 617

  Fly  1395 SSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSA 1459
            |:||.|..|..:.|.|.:...:.||:..:.....|...|||||..|||:.|:...:..:::.:..
  Fly   618 SADRCPKVLCQIFHDLRECAGEHFPSNREVRYSVVSGFIFLRFFAPAILGPKLFDLTTERLDAQT 682

  Fly  1460 KRGLMLMSKILQNIANHVEFSKEQHMLCFNDF---------LRDHFEAGRRFFIQIASDCETVDQ 1515
            .|.|.|:||.:|::.|.|. |:.....|..:|         ...|.:|.:.|.       |.:..
  Fly   683 SRTLTLISKTIQSLGNLVS-SRSSQQTCKEEFTVELYKKFCTEQHVDAVKHFL-------EVIST 739

  Fly  1516 TSHSMSFISDANVLAL-----------------------------HRLLWTHQEKIGDYLSSSRD 1551
            .||:.|.:..|...|.                             |..|.||.      ||.:
  Fly   740 PSHASSSVHPAAAAATPLEPVLLKEGLMTKYPTSRKRFGRQFKQRHFRLTTHS------LSYA-- 796

  Fly  1552 HKAVGRRPF-DKMATLLAYLGPPEHKPVDSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEF 1615
             |:.|::|. |.....:|.:...:.|.......|.......|:.:.:||..|        |:|.|
  Fly   797 -KSKGKQPICDIPLQEIASVEQLKDKSFKMQNCFKIVHNDRSLIVQTTNCVE--------EREWF 852

  Fly  1616 KTLKSMNI-------FYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVV 1673
            ..|..:.:       ::......||   ||....|   .:.|            .|.|....:..
  Fly   853 DLLHKICLMNSIRMQYFHPSAFVSG---FYSCCGR---SDEN------------SPGCKKVLDKT 899

  Fly  1674 IDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLL 1738
            :|:        |:.:.:..   :.|.:..:.:|.:.:.|.:        ..:.:|.||..::.|.
  Fly   900 MDY--------FQMDLVTA---LDPALDLQRIHTLIMSNMS--------VLESLLDPLTYHQSLS 945

  Fly  1739 FL--ESPNKLTDFIDAEQQKLPGATLSLD---EDLKVFSNALKLSHKDTKVAIKVGPTALQITSA 1798
            ..  :..|.|.......|:..|.|.....   |.|:..:.|:...|:|.|..|
  Fly   946 QTQHQQHNPLVPLATDLQKHSPQAFAEFKRTIEKLREKAYAIDRDHRDYKQGI------------ 998

  Fly  1799 EKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHN 1846
              |:.|.         |.|  .:..:.|||.:  .:...:|||:..|:
  Fly   999 --TRQLK---------YGS--RQAPIGDDNYW--HMMRAAGQLNQQHH 1031

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	91/371 (25%)
		CRAL_TRIO_2	1633..1767	CDD:463965	21/138 (15%)
		PH_NF1	1759..1868	CDD:270123	19/91 (21%)
		MOR2-PAG1_mid	<1931..>2086	CDD:433790
RasGAP1	NP_001261664.1	C2A_RasA2_RasA3	44..169	CDD:176046	7/15 (47%)
		C2B_RasA3	280..462	CDD:175977	42/209 (20%)
		RasGAP_GAP1_like	473..740	CDD:213330	77/289 (27%)
		PH_GAP1-like	763..867	CDD:269950	21/120 (18%)
		BTK	862..897	CDD:128417	8/52 (15%)