Protein Alignment Nf1 and Nf1

DIOPT Version :9

• Sequence 1: NP_733132.2
• Gene: Nf1 / 43149
• FlyBaseID: FBgn0015269
• Length: 2802
• Species: Drosophila melanogaster
• Sequence 2: XP_006532499.1
• Gene: Nf1 / 18015
• MGIID: 97306
• Length: 2852
• Species: Mus musculus
• Alignment Length: 2974
• Identity: 1600/2974 - (53%)
• Similarity: 2030/2974 - (68%)
• Gaps: 337/2974 - (11%)

  Fly     4 KPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAA 68
            :|.||..|:::||::|||.:.|...|..::|.:....|||:||:|:|||||||||.:|:.||...
Mouse     5 RPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMR 69

  Fly    69 LQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQ 133
            :......:..|...| |||.|||:||..|.|||.|.:|.|.||.||.||..|:....:.|.:||:
Mouse    70 IFGEAAEKNLYLSQL-IILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAE 133

  Fly   134 LKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETI 198
            |:..||.|||:||.|:|:|||:|||.|:||||.|||:|.|.:||||:|:|::|..||.:||:||.
Mouse   134 LRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETA 198

  Fly   199 TKFRS-KRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKS 262
            .||:: |:...|.::.|||||.|||:|.:|.||..|.:....|::.|.|.|.|.|:.| .|:.|.
Mouse   199 FKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGF-AESTKR 262

  Fly   263 KTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKR 327
            |..|||||::||||.|..::.:..::..                      :.:.:.|.|::|:::
Mouse   263 KAAVWPLQIILLILCPEIIQDISKDVVD----------------------ESNINKKLFLDSLRK 305

  Fly   328 GLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQG 392
            .|..|..|:|:|||||||||||||||||||..| |:|:|.|||..:.|||.|||||:||||||.
Mouse   306 ALAGHGGSRQLTESAAIACVKLCKASTYINWED-NSVIFLLVQSMVVDLKNLLFNPSKPFSRGS- 368

  Fly   393 YNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPF 457
             ..||::|||||.||||||:|||.:..|:||..:||..:|:|:|.||         .|:...:.|
Mouse   369 -QPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSL---------HRIITNSTF 423

  Fly   458 R--IVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLT 520
            :  :.....|.|||:.|.|:..|.|||.:|.:||:||.||..||..:|...|||.|.|.|.   .
Mouse   424 KHGLGTASALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTS---L 485

  Fly   521 RAEEGP------AHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPD 579
            :.:|.|      ::|.|||.:|:||||||.|||....|...|.||||.|||.|||.||.|:.||:
Mouse   486 KFKEKPTDLETRSYKCLLLSMVKLIHADPKLLLCNPRKQGPETQSSTAELITGLVQLVPQSHMPE 550

  Fly   580 VAQEAMEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILI 644
            ||||||||||.||..:.|::|||:||:.|||::|||:||.|.:||..||:.:.|::|||||||||
Mouse   551 VAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILI 615

  Fly   645 CRNTFLQRHKD---------YAH-VGSQIA----------------------------------- 664
            |||.||.::|.         |.: ||.:::
Mouse   616 CRNKFLLKNKQADRSSCHSLYLYGVGCEMSATGNTTQMSVDHDEFLRACTPGASLRKGRGNSSMD 680

  Fly   665 ----------ICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIMP 719
                      ||:||..|:||..:|:||:.|.:|||.::|||..|||||:|.|..||::|...:|
Mouse   681 STAGCSGTPPICRQAQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLP 745

  Fly   720 NYHIYQELAQLSTSATDSRICFFDNTHGNVLS--RLTLQKRIMTLLRKIEHCVHGVQPAWEETFR 782
            ||:.:.|.|.:|                |::|  |..||||:|.|||:|||...|...|||:|..
Mouse   746 NYNTFMEFASVS----------------NMMSTGRAALQKRVMALLRRIEHPTAGNIEAWEDTHA 794

  Fly   783 NWEVSSKVLQTYPKCKGEDGQ-AEVFHRGMGKRRASHQSSEHDLE----EQINEWANMTWFLLAL 842
            .||.::|::..|||.|.|||| ||..|:.:.|||.||.|....::    :.:.||.|||.||.||
Mouse   795 KWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCAL 859

  Fly   843 GGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSY 907
            |||||.:||||     ..:..:..:|::::......|..|..|::.
Mouse   860 GGVCLQQRSSS-----GLATYSPPMGAVSERKGSMISVMSSEGNID------------------- 900

  Fly   908 CPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNV 972
            .||::|:.:||.|:||::||:||.|:.|||:|||.|:|..|||:||::::..:.||||.|||  |
Mouse   901 SPVSRFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQ--V 963

  Fly   973 NVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYA 1037
            .::|.||||:|.||.|||::||        |.....|||||..|||.|||.:|||||.|...|:|
Mouse   964 LLSDSNTQFVEQTIAIMKNLLD--------NHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHA 1020

  Fly  1038 IRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLI 1102
            |:|||||||||||||.|||||:|.||||||||:||||||||||||:|.|   ..|...||
Mouse  1021 IQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAA---DDDIKCLT----- 1077

  Fly  1103 FRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNN 1167
             ||||||.||||.:||.||||||||.|..:||:|||.||||||||||||||||   ||.|.|...
Mouse  1078 -RDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDC---SEVEDENAQ 1138

  Fly  1168 TPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQ 1232
            |   ..|.|..:.:|.:||:.|:|||||||.||:||||||||.|||:.||||||.||||||:|||
Mouse  1139 T---GGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQ 1200

  Fly  1233 QGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFDAKHLL 1297
            ||||||||||||||||||:||:||||:.|:|||||||||||||..||.||||||||||||::|||
Mouse  1201 QGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLL 1265

  Fly  1298 SPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLL--DEEEETC 1360
            ..||||||.:|||::|.||||||||||.||||.||||:|||:|||.||:||:|.::  .:.:...
Mouse  1266 YQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRVIITSSDWQHVS 1330

  Fly  1361 FEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQ------------- 1412
            |||||.||:|:|.:|:::.||:.:|:|.|.|||:||..||.||||:||||||
Mouse  1331 FEVDPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPSQLRSVCHCLYQATCHSLLNKATVK 1395

  Fly  1413 --------VLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKI 1469
                    |:|:|||   ||:|||||:.:||||||||||||.|.||:||:.....:|||.||||:
Mouse  1396 ERKENKKSVVSQRFP---QNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKV 1457

  Fly  1470 LQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRL 1534
            ||:|||||.|:||:||..||||::.:|:..||||:.|||||.|.|..:||:|||||.||||||||
Mouse  1458 LQSIANHVLFTKEEHMRPFNDFVKSNFDLARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRL 1522

  Fly  1535 LWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPV-DSHMMFSSYARWSSIDMSST 1598
            ||.:|||||.||||:||||||||||||||||||||||||||||| |:|        |||::::|:
Mouse  1523 LWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTH--------WSSLNLTSS 1579

  Fly  1599 NFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLK 1663
            .|||.|.:||:|||||||.||:::|||||||||:|.|:|||:|||:|.|:.|||||||||:||||
Mouse  1580 KFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLK 1644

  Fly  1664 PFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRIL 1728
            |:...|:|:|:|.|||...|||:|:||.|||.|.|..||:||.||||||||||||||||:|:|:|
Mouse  1645 PYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLL 1709

  Fly  1729 APLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSHKDTKVAIKVGPTAL 1793
            ..|||:::|:|::.|.||.:.|:.||||||.|||:|:||||||.|||||:||||||:||||.||:
Mouse  1710 TGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAV 1774

  Fly  1794 QITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHNDCDNIVQAIIHI 1858
            |:||||:||||..||.|||:||||||||:||||:|||||:|.|:...|:|:|.:|:.|||:||||
Mouse  1775 QVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHI 1839

  Fly  1859 RNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLCALTATFDLKIEGQL 1923
            |.|||||||||:..|.|||||||||||||:|||||||.||:||:||||||||||.||:|||||||
Mouse  1840 RTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQL 1904

  Fly  1924 LETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHLCLEYMTPWLKNLV 1988
            |||.|||||:|||:||.|:|:.||.||||||||||||.|.||.:|:|||||||||||||||.|||
Mouse  1905 LETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLV 1969

  Fly  1989 KFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDMVLDNFLHKSITYG 2053
            :|||.|||:|:.:|:.||||||.:||::|:||||:|||||||:|||.:|:|:|||:|:..|.|.|
Mouse  1970 RFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGG 2034

  Fly  2054 LGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHMMWDDIAILGRYLLM 2118
            |||.:.|:||||||||||.||:|||.|||.|:|:::||:|.:||..||||:||||||||.||:||
Mouse  2035 LGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLM 2099

  Fly  2119 LSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTNPSFSEEAQRVLRLS 2183
            |||||.||||..:|||||.:||||.:|.||:|||||||:||||||||||:...||||.::|||||
Mouse  2100 LSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLLINIIHSLCTCSQLHFSEETKQVLRLS 2164

  Fly  2184 LDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADRERLSLPSLEVITDA 2248
            |.|||||||||||||||||||||.|||||.|         :|...|...:||..:|.|||.:|:|
Mouse  2165 LTEFSLPKFYLLFGISKVKSAAVIAFRSSYR---------DRSFSPGSYERETFALTSLETVTEA 2220

  Fly  2249 LLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSVTDHEVKQLLRILVK 2313
            |||||||||||:|.|:||:.||.||:.|||.|||:||||||:|:|||||.|:..::||::|||.|
Mouse  2221 LLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSK 2285

  Fly  2314 ALES-------FNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQLDEITLYGAGLALL 2371
            ||||       :|..:|||:.|:.||::||||..:||:|:||||||::||||||:.||.||.|||
Mouse  2286 ALESCLKGPDTYNSQVLIESTVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALL 2350

  Fly  2372 EQNLHTLKSQGCFDKKETIAEVMMKTREKLEWHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHPT 2436
            |||||||.|...|:.|.. .||.|..|..||||.||:||.|||:|.|||:|||||||:||:|||:
Mouse  2351 EQNLHTLDSLRIFNDKSP-EEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPS 2414

  Fly  2437 PTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSRCHVKHALPRWPADLS 2501
            |..|:||.|:|..||.::.|..:.|||||...|||||.||:.|||||||||.:||.......|:
Mouse  2415 PAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDI- 2478

  Fly  2502 SSVEN-------------------------------GEASGGVQAIGLPLSRRQKSWDILDQSAL 2535
             |:||                               |..:....|:|....|.:||.. ||...
Mouse  2479 -SMENVPMDTYPIHHGDPSYRTLKETQPWSSPKGSEGYLAATYPAVGQTSPRARKSMS-LDMGQ- 2540

  Fly  2536 QFARQHKVPTLQNARVLFKTQRSFS--VPTTKDPNNATGIEERQERGS----------------- 2581
                    |:..|.:.|..|::||.  :..||.|       :|||..|
Mouse  2541 --------PSQANTKKLLGTRKSFDHLISDTKAP-------KRQEMESGITTPPKMRRVAETDYE 2590

  Fly  2582 -----------------RSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKYSSDEGETRVLYQ 2629
                             :.||| |||||||.|||.|..||||:||||||||||::||.:.|:||:
Mouse  2591 MAETQRIPSSQQHPHLRKVSVS-ESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYE 2654

  Fly  2630 YLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASED-PSQQQLHFLQSC 2693
            ||||.||||||||||:|:|||.|||.:||:..|..:||.:..|:|:::..|: |.|.|..:|||.
Mouse  2655 YLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSF 2719

  Fly  2694 GFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLPG------DESAPVPPSPRPYNLSSSLS 2752
            ||.|||||||||:|...:.:.:||.|..|:|:::|.|||      :||...|.||.|..|.|.|
Mouse  2720 GFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPALQSQL- 2783

  Fly  2753 SLTLGSPTDKAFSSESLDFYDNCPGSVSSLRRASHSKSRAKHRINDSPS 2801
            |:|...               |...|::||..:.||....|..:..||:
Mouse  2784 SITANL---------------NLSNSMTSLATSQHSPGLDKENVELSPT 2817

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	231/355 (65%)
		CRAL_TRIO_2	1633..1767	CDD:404584	84/133 (63%)
		PH_NF1	1759..1868	CDD:270123	78/108 (72%)
		MOR2-PAG1_mid	<1931..>2109	CDD:372971	121/177 (68%)
Nf1	XP_006532499.1	RasGAP_Neurofibromin	1210..1563	CDD:213332	246/375 (66%)
		CRAL_TRIO_2	1614..1748	CDD:372686	87/133 (65%)
		PH_NF1	1740..1849	CDD:270123	74/108 (69%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167842146
Domainoid	1	1.000	1233	1.000	Domainoid score	I11
eggNOG	1	0.900	-	-		E1_KOG1826
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H226
Inparanoid	1	1.050	2904	1.000	Inparanoid score	I19
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG52953
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0005399
OrthoInspector	1	1.000	-	-		oto94593
orthoMCL	1	0.900	-	-		OOG6_103500
Panther	1	1.100	-	-	LDO	PTHR10194
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R472
SonicParanoid	1	1.000	-	-		X4961
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.790