DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nf1 and Rasa2

DIOPT Version :9

Sequence 1:	NP_733132.2	Gene:	Nf1 / 43149	FlyBaseID:	FBgn0015269	Length:	2802	Species:	Drosophila melanogaster
Sequence 2:	XP_006510838.1	Gene:	Rasa2 / 114713	MGIID:	2149960	Length:	884	Species:	Mus musculus

Alignment Length:	639	Identity:	131/639 - (20%)
Similarity:	236/639 - (36%)	Gaps:	175/639 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly  1266 PIAMALANVVTTSQMDELARV--LVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKI 1328
            |::.:.|.::.....|:...|  ||.|....:.|.|.:..:...:::.:.....:||||||.::.
Mouse   377 PVSASAAYILGEICQDQKDAVLPLVRLLLHHNKLVPFITAVAELDLKDTPDANAIFRGNSLATQC 441

  Fly  1329 MAFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAII 1393
            :....||.|..||::.|:|::..:. |..::| |:||.:|...:::|.::.||.....|||:.|:
Mouse   442 LTEMMKIVGGHYLKVTLKPVLDEIC-ESSKSC-EIDPVKLKEGDNVENNKENLYYYVDKVFNTIV 504

  Fly  1394 NSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSS 1458
            .||...|..:..:.:.|.|:.:|:|||.......||.:.:||||...||:||....:......:.
Mouse   505 GSSVSCPTVMCDIFYSLRQMAAKKFPNHPHVQYSAVSSFVFLRFFAVAILSPHAFHLRPHYPDTQ 569

  Fly  1459 AKRGLMLMSKILQNIAN-------HVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQT 1516
            ..|.|.|:||.:|.|.|       ...|.|.  ::|  :||:...|  .|:|..:.   :.:|:.
Mouse   570 TVRTLTLISKTIQIIGNWGCQSRKKSRFKKS--VMC--EFLKMFQE--ERYFTDVK---KFLDEI 625

  Fly  1517 SHSMSFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSH 1581
            |.:.:..|......:|.       |.|:....::....:|::.|.|
Mouse   626 SSTETKESSGTSEPVHL-------KEGEMYKRAQGRTRIGKKNFKK------------------- 664

  Fly  1582 MMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKI 1646
                   ||..:......:      |:...|:...|:...||.               ...:.:.
Mouse   665 -------RWFCLTSRELTY------HRQQGKDAIYTIPVKNIL---------------AVEKLEE 701

  Fly  1647 GETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQ--------KWFYVLPTVAYE 1703
            |..|.               .:.|:|:    ||     .:|.::|        :|..||..|:
Mouse   702 GSFNK---------------KNMFQVI----HT-----EKTLYIQANNCVEANEWIDVLCRVS-- 740

  Fly  1704 NVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPG---ATLSLD 1765
                    .||.  ...:.||..  |.|.||  .|..:..::.|          ||   .|..:.
Mouse   741 --------RCNH--NRLSSFHPS--AYLNGN--WLCCQETSEST----------PGCKPCTAGIP 781

  Fly  1766 EDLKVFSNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQF 1830
            .|:::                       .|....:|:.: :|:....:....::||.|      .
Mouse   782 ADIQI-----------------------DIDEDRETERI-YSIFTLSLLKLQKMEETC------G 816

  Fly  1831 TLSI----TNESGQLSFIHNDCDNIVQAIIHIRNRWE-LSQPDSVTVHQKIRPK 1879
            ::::    ..|.|...|...|.....:.|..|::..| |.:|     |:|.|.|
Mouse   817 SIAVYQGPQKEPGYSKFTIEDSVATFKTIQQIKSTIEKLDEP-----HEKYRKK 865

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	79/317 (25%)
		CRAL_TRIO_2	1633..1767	CDD:404584	25/144 (17%)
		PH_NF1	1759..1868	CDD:270123	17/116 (15%)
		MOR2-PAG1_mid	<1931..>2109	CDD:372971
Rasa2	XP_006510838.1	C2A_RasA2_RasA3	82..195	CDD:176046
		C2B_RasA3	208..350	CDD:175977
		RasGAP	359..629	CDD:383031	72/262 (27%)
		PH_GAP1m_mammal-like	626..758	CDD:241521	33/223 (15%)
		BTK	743..778	CDD:128417	11/50 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R472
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.940