Protein Alignment Nf1 and IQGAP2

DIOPT Version :9

• Sequence 1: NP_733132.2
• Gene: Nf1 / 43149
• FlyBaseID: FBgn0015269
• Length: 2802
• Species: Drosophila melanogaster
• Sequence 2: NP_006624.3
• Gene: IQGAP2 / 10788
• HGNCID: 6111
• Length: 1575
• Species: Homo sapiens
• Alignment Length: 2006
• Identity: 371/2006 - (18%)
• Similarity: 661/2006 - (32%)
• Gaps: 713/2006 - (35%)

  Fly    45 ISRYRFSLVISGLTKMLQRVNEAALQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMN 109
            :.|.|:..::.......:.::|...||......|:.|.   ....:|.||..:...|...||.:.
Human     9 LQRPRYGSIVDDERLSAEEMDERRRQNIAYEYLCHLEE---AKRWMEVCLVEELPPTTELEEGLR 70

  Fly   110 VKLLLREISQFVDVQSDSNPNAAQLKALASKVLFALSQNHFSAV---FNRISARIQELTSCSEEN 171
            ..:.|.::::|.           ..|.::.|.::.:.|..:...   |......:|.|.:
Human    71 NGVYLAKLAKFF-----------APKMVSEKKIYDVEQTRYKKSGLHFRHTDNTVQWLRA----- 119

  Fly   172 PDYNDIELIQHIDMDMIKLTKLL-QETITKFRSKRAPPLI-LLYSLEKAIWNWIEYHPQEFQDLQ 234
                         |:.|.|.|:. .||...:..|..|.:| .:::|...::. :...|| .|||.
Human   120 -------------MESIGLPKIFYPETTDVYDRKNIPRMIYCIHALSLYLFK-LGIAPQ-IQDLL 169

  Fly   235 RG---TNRDISTCWEPLMDFVEYFKTENKKSKTLVWPL------------------------QML 272
            ..   |..:||...:.|..:.....:.:|....|...|                        |.:
Human   170 GKVDFTEEEISNMRKELEKYGIQMPSFSKIGGILANELSVDEAALHAAVIAINEAVEKGIAEQTV 234

  Fly   273 LLILNPSCLEAVVNELQQSEKEKE------KDKEKVASKSAQSTSRDKDFSAKQFIESIKRGLGQ 331
            :.:.||:.:..:|::....|.:||      |.:|....|::..:..::|    .:.|.:.:...|
Human   235 VTLRNPNAVLTLVDDNLAPEYQKELWDAKKKKEENARLKNSCISEEERD----AYEELLTQAEIQ 295

  Fly   332 HSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQGYNFA 396
            .:.:| |...||:..:     :..|...|..|.:..|               .||
Human   296 GNINK-VNRQAAVDHI-----NAVIPEGDPENTLLAL---------------KKP---------- 329

  Fly   397 DIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVD--FRLQNKNPFRI 459
                                ||       ..|..|.|.        |.:|.:  |.||.:|
Human   330 --------------------EA-------QLPAVYPFA--------AAMYQNELFNLQKQN---- 355

  Fly   460 VNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLTRAEE 524
                                             |..|:||                       ||
Human   356 ---------------------------------TMNYLAH-----------------------EE 364

  Fly   525 GPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEAMEALL 589
                   ||:.|.::.|  ..|||           ..|| .|.|||:.:|...|         .:
Human   365 -------LLIAVEMLSA--VALLN-----------QALE-SNDLVSVQNQLRSP---------AI 399

  Fly   590 ALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHK 654
            .|:..:|..|   |...||...|..:||......|..::|.|..|::          |..:|...
Human   400 GLNNLDKAYV---ERYANTLLSVKLEVLSQGQDNLSWNEIQNCIDMV----------NAQIQEEN 451

  Fly   655 DYAHVGSQIAICKQAHIKMEVVFFMYL-WSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIM 718
            |                  .||...|: .::|....|.:|..  ||...|.|   ||      :.
Human   452 D------------------RVVAVGYINEAIDEGNPLRTLET--LLLPTANI---SD------VD 487

  Fly   719 PNY--HIYQEL------AQLSTSATDSRICFFDNTH-----GNVLSRLTLQKRIMTLLRKIEHCV 770
            |.:  | ||::      .:|..|.:.|::.:.|...     .||  .....|:.:||:..:..|:
Human   488 PAHAQH-YQDVLYHAKSQKLGDSESVSKVLWLDEIQQAVDDANV--DKDRAKQWVTLVVDVNQCL 549

  Fly   771 HGVQPAWEETFRNWEVSSKVLQT-----------YPKCKGEDGQAEVFHRGMGKRRASHQSS--E 822
            .|            :.||.:|..           .|:|..:...|.|..:.:...|.|...|  :
Human   550 EG------------KKSSDILSVLKSSTSNANDIIPECADKYYDALVKAKELKSERVSSDGSWLK 602

  Fly   823 HDLEEQINEWAN-----MTWFLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSS--- 879
            .:|.::.:.:.|     .:|   .....||:|.|......::......::|.:.:| ::|:|
Human   603 LNLHKKYDYYYNTDSKESSW---VTPESCLYKESWLTGKEIEDIIEEVTVGYIREN-IWSASEEL 663

  Fly   880 ------TSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQ----- 933
                  ||||                         |:.:...:..:..:...|:..:.||
Human   664 LLRFQATSSG-------------------------PILREEFEARKSFLHEQEENVVKIQAFWKG 703

  Fly   934 -KNVKELVGEEMSTQLYPILFDQVRAIV--EKFFDQQGQVNVNVTDINTQFIEHTIYIMK--SIL 993
             |..||.:....:      ..|...:||  :.:| :......:.......|.:|...|:|  |:|
Human   704 YKQRKEYMHRRQT------FIDNTDSIVKIQSWF-RMATARKSYLSRLQYFRDHNNEIVKIQSLL 761

  Fly   994 DPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVR-YVRHLDMTVYAIRIKTKLCQLVEVMMKRRDD 1057
              :|||       :..::..:...|...|.::| :|..||                      :.|
Human   762 --RANK-------ARDDYKTLVGSENPPLTVIRKFVYLLD----------------------QSD 795

  Fly  1058 LAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLP 1122
            |.|::|::......|.:|                  .|..|..|
Human   796 LDFQEELEVARLREEVVT------------------KIRANQQL--------------------- 821

  Fly  1123 LQPEESDRGDLMDAKSALFLKYFTLFMN--LLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTAL 1185
                |.|. :|||.|..|.:|      |  .|.|.|..|   |::|
Human   822 ----EKDL-NLMDIKIGLLVK------NRITLEDVISHS---KKLN------------------- 853

  Fly  1186 RNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFE 1250
                                            :.:...||:|.....||.:..:.......:.::
Human   854 --------------------------------KKKGGEMEILNNTDNQGIKSLSKERRKTLETYQ 886

  Fly  1251 QLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFD------AKHLLSPLLWNMFYREV 1309
            ||..|:    ....|.:|..:..:........:..|:.||::      .::||..|.......|:
Human   887 QLFYLL----QTNPLYLAKLIFQMPQNKSTKFMDTVIFTLYNYASNQREEYLLLKLFKTALEEEI 947

  Fly  1310 EVS-DCMQTLFRGNSLGSK-IMAFCFKIYGASYLQMLLEPLIRPLLDEE---------------- 1356
            :.. |.:|.:..||....| :::|.....|.:.|:.||.|:::.::|::
Human   948 KSKVDQVQDIVTGNPTVIKMVVSFNRGARGQNTLRQLLAPVVKEIIDDKSLIINTNPVEVYKAWV 1012

  Fly  1357 ---EETCFEVDPARLDPTED-----------IEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMC 1407
               |....|......|.|.:           :|....||..:|.||.::||:|.|..|..||.:.
Human  1013 NQLETQTGEASKLPYDVTTEQALTYPEVKNKLEASIENLRRVTDKVLNSIISSLDLLPYGLRYIA 1077

  Fly  1408 HCLYQVLSKRFPNLLQNN-IGAVGTVIFLRFINPAIVSPQELGIVDK----QVHSSAKRGLMLMS 1467
            ..|...:.::||:..::. :..||.:::.|::|||||:|....|:|.    |::|..:|.|..::
Human  1078 KVLKNSIHEKFPDATEDELLKIVGNLLYYRYMNPAIVAPDGFDIIDMTAGGQINSDQRRNLGSVA 1142

  Fly  1468 KILQNIANHVEFSKE-QHMLCFNDFLRDHFEAGRRFFIQIA--------------SDCETVDQTS 1517
            |:||:.|::..|..| :|:...|::|.:.::..|::|.:..              :|..||   |
Human  1143 KVLQHAASNKLFEGENEHLSSMNNYLSETYQEFRKYFKEACNVPEPEEKFNMDKYTDLVTV---S 1204

  Fly  1518 HSMSFISDANVLALHRLLWTHQEKI----GDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPV 1578
            ..:.:||...:::.|.||..||:.|    .|.||....  ::|..|     |:.::||.....|.
Human  1205 KPVIYISIEEIISTHSLLLEHQDAIAPEKNDLLSELLG--SLGEVP-----TVESFLGEGAVDPN 1262

  Fly  1579 DSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARR 1643
            |.:.. ::.::.|..::|       :|....::.|:.:.:.|.::
Human  1263 DPNKA-NTLSQLSKTEIS-------LVLTSKYDIEDGEAIDSRSL-------------------- 1299

  Fly  1644 YKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENV-HA 1707
                          :|.|.|        ::||.......|.. ||.|:     .|..|.:.| ||
Human  1300 --------------MIKTKK--------LIIDVIRNQPGNTL-TEILE-----TPATAQQEVDHA 1336

  Fly  1708 VYIYN---CNSWVREYTKFHDRIL----APL-----KGNRKLLFLE------SPNKLTDFID--- 1751
            ..:.:   .:|...|..|....::    .||     |..|.|..||      |.||..|.::
Human  1337 TDMVSRAMIDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRTLEQTGHVSSENKYQDILNEIA 1401

  Fly  1752 --------------AEQQKLPGATLSL-------DEDLKVFSNALK-----LSHKDTKVAIKVGP 1790
                          ||..||.....:|       :|.:..:...:|     |..|:|:.:||:..
Human  1402 KDIRNQRIYRKLRKAELAKLQQTLNALNKKAAFYEEQINYYDTYIKTCLDNLKRKNTRRSIKLDG 1466

  Fly  1791 TALQITSAEKTKVLAHSVLLNDVYYASEIEE--VCL-VDDNQFTLSITNESGQLSF 1843
            .. :...|::.|.:.        |.|:::.|  |.| :||.|     ||:...::|
Human  1467 KG-EPKGAKRAKPVK--------YTAAKLHEKGVLLDIDDLQ-----TNQFKNVTF 1508

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	90/394 (23%)
		CRAL_TRIO_2	1633..1767	CDD:404584	33/176 (19%)
		PH_NF1	1759..1868	CDD:270123	21/100 (21%)
		MOR2-PAG1_mid	<1931..>2109	CDD:372971
IQGAP2	NP_006624.3	CH_IQGAP2	11..166	CDD:409124	33/188 (18%)
		IQ	689..710	CDD:197470	5/20 (25%)
		IQG1	<784..1575	CDD:227586	183/935 (20%)
		RasGAP	916..1274	CDD:413381	87/368 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R472
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	1.030