DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nf1 and nf1

DIOPT Version :9

Sequence 1:	NP_733132.2	Gene:	Nf1 / 43149	FlyBaseID:	FBgn0015269	Length:	2802	Species:	Drosophila melanogaster
Sequence 2:	XP_002936865.2	Gene:	nf1 / 100493879	XenbaseID:	XB-GENE-488024	Length:	2785	Species:	Xenopus tropicalis

Alignment Length:	2921	Identity:	1584/2921 - (54%)
Similarity:	2008/2921 - (68%)	Gaps:	298/2921 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 KPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAA 68
            ||.||..|::.||::|||.:.....|..::|.:....|||:||:|:|||||||||.:|:.||...
Frog     5 KPVEWVQAVVNRFDEQLPIKASQQNTHTKVSMEHNKECLINISKYKFSLVISGLTNILKNVNHMR 69

  Fly    69 LQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQ 133
            :......:..|...| |||.||::||..|.||:.|.:|.|.||.||.||..|:....:.|..||:
Frog    70 ISGETSEKNLYLSQL-IILDTLDKCLAGQPKDSMRLDETMLVKQLLPEICHFIHTYREGNQYAAE 133

  Fly   134 LKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETI 198
            |:..||.|||:||.|:|:|||:|||.|:||||.|||:|.|.:||:|||:|::|..||.:|||||:
Frog   134 LRMSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNADVHDIDLIQYINVDCSKLKRLLQETV 198

  Fly   199 TKFRS-KRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKS 262
            .||:| |:...|.::.|||||.|||:|.:|.||..|.:....|::.|.|.|.|.|:.|...||:
Frog   199 FKFKSLKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQRPQTDMADCAEKLFDLVDSFAESNKR- 262

  Fly   263 KTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKR 327
            |..|||||::||:|.|..::.:..::.:..|                      .:.|.|:|::::
Frog   263 KAAVWPLQIILLVLCPEIIQDISKDVVEESK----------------------MNKKLFLENLRK 305

  Fly   328 GLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQG 392
            .|..|..:|.:||:||||.|||||||||||..| |:|:|.|||..:.|||.|||||:||||||..
Frog   306 ALAGHGGTKSLTETAAIASVKLCKASTYINWED-NSVIFHLVQSLVIDLKNLLFNPSKPFSRGAV 369

  Fly   393 YNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPF 457
            ...||::|||||.||||||||||.:..|.||..|||..:|:|:|.||                 .
Frog   370 SQNADLDLMIDCLVSCFRINPHNNQHFKTCLAQSSPTTFHYVLVNSL-----------------H 417

  Fly   458 RIVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQ---KGL 519
            ||:....|.|||:.|.|:..|.|||::|::||....|    .|..|...|||.|.|...   |..
Frog   418 RIITNSALDWWPKIDAVYCYSGELRSMFSETLKLVMQ----QTASRLTPSLTFKEKMPYLKFKDR 478

  Fly   520 TRAEEGPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEA 584
            ....|..::|.|||.:|:|||.||.|||...||...:.||||.|||||||.||.|:.||::||||
Frog   479 LADPETKSYKYLLLSVVKLIHGDPKLLLYNPGKAVSDTQSSTAELINGLVQLVPQSNMPEIAQEA 543

  Fly   585 MEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTF 649
            |||||.||..:.||:|||||||.|||::|||:|:||..||..||:.|.|::||||||||||||.|
Frog   544 MEALLVLHQSDSIELWNPEAPIETFWEISSQMLYSICTKLTGHQLLNSTEILKWLREILICRNKF 608

  Fly   650 LQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTV 714
            |.::|:.|..||.|.||:||..|:||..:|:|||.|.:|||.::|||..|||||:|.|..||::|
Frog   609 LLKYKESAAAGSGIPICRQAQTKLEVALYMFLWSPDTEAVLVAMSCFRSLCEEADIRCGVDEVSV 673

  Fly   715 GFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLS--RLTLQKRIMTLLRKIEHCVHGVQPAW 777
            ...:|||:.:.|.|.:|                |::|  |..||||:|.|||:|||...|...||
Frog   674 HNFLPNYNTFMEFASVS----------------NMVSTGRAALQKRVMALLRRIEHPTAGNTEAW 722

  Fly   778 EETFRNWEVSSKVLQTYPKCKGEDGQA-EVFHRGMGKRRASHQSSEHDLE----EQINEWANMTW 837
            |:|...|:.:::::..|||.|.||||| |..|:.:.|||.||.|....::    :.:.||.|||.
Frog   723 EDTHAKWDHATRLILNYPKTKLEDGQAPESLHKTIVKRRMSHVSGGGSIDLSDTDCLQEWINMTG 787

  Fly   838 FLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLA---------QNSLYSSSTSSGHGSLHPSTVS 893
            ||.|||||||            |.:.||.|.:.:         :.|:.|..::.|:..
Frog   788 FLSALGGVCL------------QHRGNAGLVTYSPPMGPVNERKGSVISMVSTEGNAE------- 833

  Fly   894 LSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRA 958
                          .||::|:.:||.|:||::||:|:.|:.|:|:|||.|:|..|||:||::::.
Frog   834 --------------TPVSKFLDRLLSLMVCNHEKVGIQIRNNIKDLVGLELSPALYPMLFNKMKN 884

  Fly   959 IVEKFFDQQGQVNVNVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLG 1023
            .:.||||.|||  |.:||.||||:|.||.|:|::||        |.....|||||..|:|.|||.
Frog   885 NISKFFDSQGQ--VLLTDTNTQFVEQTITILKNLLD--------NHTEGSSEHLGQASLETMMLN 939

  Fly  1024 IVRYVRHLDMTVYAIRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPP 1088
            :|||||.|...|:||:|||||||||||||:|||||:|.||||||||:||||||||||||:|.|
Frog   940 LVRYVRVLGNLVHAIQIKTKLCQLVEVMMERRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAA-- 1002

  Fly  1089 SSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLN 1153
             ..|...||      ||||||.||||.:||.||||||||.|..:||:|||.||||||||||||||
Frog  1003 -DEDVKCLT------RDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLN 1060

  Fly  1154 DCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQ 1218
            ||   ||||.:...|   ..|.|..:.:|.:||:.|:|||||||.||:||||||||.|||:.|||
Frog  1061 DC---SEAEDDGTQT---GGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQ 1119

  Fly  1219 TRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDEL 1283
            |||.||||||:|||||||||||||||||||||:||:||||:.|:|||||||||||||..||.|||
Frog  1120 TRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDEL 1184

  Fly  1284 ARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPL 1348
            |||||||||::|||..||||||.:|||::|.||||||||||.||||.||||:|||:|||.|||||
Frog  1185 ARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLEPL 1249

  Fly  1349 IRPLL--DEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLY 1411
            :|..:  .|.:...||||..||:..|.:|:::..|:.:|:|.|.|||||::.||.||||:|||||
Frog  1250 LRTFITSPEWQYVSFEVDSTRLEGNELLEENQRRLLQMTEKFFHAIINSTNEFPTQLRSVCHCLY 1314

  Fly  1412 Q---------------------VLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQV 1455
            |                     |:|:|||   ||:|||||:.:||||||||||||.|.||:||:.
Frog  1315 QATCHSLLSKATVKEKKENKKSVVSQRFP---QNSIGAVGSAMFLRFINPAIVSPYEAGILDKKP 1376

  Fly  1456 HSSAKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSM 1520
            ....:|||.|||||||:|||||.|:||:||..||:|::.:|:|.||||..||::|...|..:||:
Frog  1377 PPRLERGLKLMSKILQSIANHVLFTKEEHMRPFNEFVKSNFDAARRFFFDIANNCPPSDTVNHSL 1441

  Fly  1521 SFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPV-DSHMMF 1584
            |||:|.||||||||||.:|||||.||||:||||||||||||||||||||||||||||| |:|
Frog  1442 SFITDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTH--- 1503

  Fly  1585 SSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGET 1649
                 |||::::|:.|||.|.:||:|||||||.||::|||||||||::|.|||||||||:|.|:.
Frog  1504 -----WSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLNIFYQAGTSRAGNPVFYYIARRFKTGQI 1563

  Fly  1650 NGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCN 1714
            |||||||||:|||||:...|:|:|:|.||....|||:|:||.|||.|.|..|||||.||||||||
Frog  1564 NGDLLIYHVLLTLKPYYAKPYEIVVDLTHAGPSNRFKTDFLSKWFVVFPGFAYENVTAVYIYNCN 1628

  Fly  1715 SWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSH 1779
            ||||||||:|:|:|..|||:::|:|::...|||::|:.||||||.|||:|:||||:|.|||||:|
Frog  1629 SWVREYTKYHERLLTGLKGSKRLIFIDPSGKLTEYIEHEQQKLPAATLALEEDLKLFHNALKLAH 1693

  Fly  1780 KDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFI 1844
            |||||:||||.||:|:||||:|:||..||.|||:||||||||:||||:|||||:|.|:...|:|:
Frog  1694 KDTKVSIKVGSTAVQVTSAERTRVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFM 1758

  Fly  1845 HNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLC 1909
            |.:|:.|||:|||||.|||||||||:..|.|||||||||||||:|||||||.||:||:|||||||
Frog  1759 HQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLC 1823

  Fly  1910 ALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKH 1974
            |||.||:||||||||||.|||||:|||:||.|:|:.||.||||||||||||.|.||.:|:|||||
Frog  1824 ALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKH 1888

  Fly  1975 LCLEYMTPWLKNLVKFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELID 2039
            ||||||||||.|||:|||..||:|:.:||.||||||.:||::|:||||:|||||||:|||.:|:|
Frog  1889 LCLEYMTPWLLNLVRFCKLTDDAKRQRVSAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLD 1953

  Fly  2040 MVLDNFLHKSITYGLGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHM 2104
            :|||:|:..|.|.||||.:.|:||||||||||.||:|||.|||.|:|:::||:|.:||..||||:
Frog  1954 VVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHL 2018

  Fly  2105 MWDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTN 2169
            ||||||||.||:|||||||.||||..:|||||.:|.||.:|.||:||||||||||||||||||:.
Frog  2019 MWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTLLVATGPLSLRASTHGLVINIIHSLCTCSQ 2083

  Fly  2170 PSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADR 2234
            .:||||.::||||||.|||||||||||||||||||||.|||||.|         :|...|...:|
Frog  2084 LNFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYR---------DRSFSPGSYER 2139

  Fly  2235 ERLSLPSLEVITDALLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSV 2299
            |..:|.|||.:|:||||||||||||:|.|:||:.||.||:.|||.|||:||||||:|:|||||.|
Frog  2140 ETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQKFAFQYNPSLQPRALVVFGCISKRV 2204

  Fly  2300 TDHEVKQLLRILVKALES-------FNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQ 2357
            :..::||::|||.|.|||       :|..:||||.|:.||::||||..:||:|:||||||::|||
Frog  2205 SHGQIKQIIRILSKGLESCLKGPDNYNSQVLIEATVIALTKLQPLLNKDSPMHKALFWVAMAVLQ 2269

  Fly  2358 LDEITLYGAGLALLEQNLHTLKSQGCFDKKETIAEVMMKTREKL-EWHFKQLDHAVGLSFRSNFH 2421
            |||:.||.||.||||||||||.|...|:.|.. .||.|:.|..| |||.||:||.|||:|.|||:
Frog  2270 LDEVNLYSAGTALLEQNLHTLDSLRVFNDKSP-EEVFMEIRNPLEEWHCKQMDHFVGLNFNSNFN 2333

  Fly  2422 FALVGHLIKGFRHPTPTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSR 2486
            |||||||:||:|||:||||:||.|:|..||.::.|..:.|||||..:|||||.||:.||||||||
Frog  2334 FALVGHLLKGYRHPSPTTVARTIRILHTLLALVNKHRNCDKFEVNTESVAYLAALLTVSEEVRSR 2398

  Fly  2487 CHVKHALPRWPADLS------------------SSVENGEASGGVQAIGLPLS-----------R 2522
            |.:||.......|:|                  .:::..:.....||.|:.|:           |
Frog  2399 CSLKHRKSLLLTDVSMENVPMDTYPVHHNDPTCRTLKENQPWSSPQASGMHLAASYPTVGQVSPR 2463

  Fly  2523 RQKSWDILDQSALQFARQHKVPTLQNARVLFKTQRSFS--VPTTKDPNNATGIEERQERGS---- 2581
            .:||.. ||...         |:..|.:.|..|::||.  :..||.|       :|||..|
Frog  2464 TRKSMS-LDMGQ---------PSQANTKKLLGTRKSFDHLISDTKAP-------KRQEMESGITT 2511

  Fly  2582 -----------------------------RSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKY 2617
                                         :.||| ||.||||.|||.|...|||:||||||||||
Frog  2512 PPKMRRVAEHDYDIDTPRISSPQQHPHLRKVSVS-ESTVLLDEEVLTDPKTQALLLTVLATLVKY 2575

  Fly  2618 SSDEGETRVLYQYLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASED- 2681
            ::||.:.|:||:||||.||||||||||:|:|||.||:.:||:..|..:||.:..|:|:::..|:
Frog  2576 TTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKISTLLSLCQDPNLLNPIHGIVQSVVYHEES 2640

  Fly  2682 PSQQQLHFLQSCGFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLP------GDESAPVPP 2740
            |.|.|..:|||.||.|||||||||:|...:.:.:||.|..|:|:::..||      .:||...|.
Frog  2641 PPQYQPSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDKYLPVIDEEASEESLLTPT 2705

  Fly  2741 SPRPYNLSSSLSSLTLGSPTDKAFSSESLDFYDNCPGSVSSLRRASHSKSRAKHRINDSPS 2801
            ||.|..:.|.| |:|...               |...|::||..:.||....|..:..||:
Frog  2706 SPYPPAVQSQL-SITANL---------------NLSNSMTSLATSQHSPGIDKENVELSPT 2750

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	227/355 (64%)
		CRAL_TRIO_2	1633..1767	CDD:404584	86/133 (65%)
		PH_NF1	1759..1868	CDD:270123	76/108 (70%)
		MOR2-PAG1_mid	<1931..>2109	CDD:372971	121/177 (68%)
nf1	XP_002936865.2	RasGAP_Neurofibromin	1143..1496	CDD:213332	227/355 (64%)
		CRAL_TRIO_2	1547..1681	CDD:372686	86/133 (65%)
		PH_NF1	1673..1782	CDD:270123	76/108 (70%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	1238	1.000	Domainoid score	I11
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H226
Inparanoid	1	1.050	2891	1.000	Inparanoid score	I21
OMA	1	1.010	-	-		QHG52953
OrthoDB	1	1.010	-	-		D4905at33208
OrthoFinder	1	1.000	-	-		FOG0005399
OrthoInspector	1	1.000	-	-		oto104792
Panther	1	1.100	-	-	LDO	PTHR10194
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4961
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	11.080