DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Nf1 and rasa3

DIOPT Version :9

Sequence 1:	NP_733132.2	Gene:	Nf1 / 43149	FlyBaseID:	FBgn0015269	Length:	2802	Species:	Drosophila melanogaster
Sequence 2:	XP_009303503.1	Gene:	rasa3 / 100318936	ZFINID:	ZDB-GENE-090313-21	Length:	853	Species:	Danio rerio

Alignment Length:	676	Identity:	139/676 - (20%)
Similarity:	242/676 - (35%)	Gaps:	193/676 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   926 EKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDI-NTQFIEHTIYIM 989
            |:.||.:.::||..:||..:...||        ...|..|....||::..:: .|:.||.:
Zfish     4 EEEGLRVFQSVKIKIGEAKNIPPYP--------GPNKMRDCYCTVNLDQEEVFRTKTIEKS---- 56

  Fly   990 KSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYAIRIKTKLCQLVEVMMKR 1054
               |.|...:|                   ....|.|..|||...::...:..:...:.:|.:|:
Zfish    57 ---LCPFYGED-------------------FYCEIPRSFRHLSFYIFDRDVFRRDSSIGKVAVKK 99

  Fly  1055 RD------------------DLAFRQEMKFRNKLVEYLTDWVMGTSHQIAP-------------- 1087
            .|                  |...:.::....:|.|.:|| ..|..|::|.
Zfish   100 EDLQKYHGKDHWFPLQPVCADSEVQGKVHLELRLSELITD-SGGLCHKLATRVLECQDLPIVNGQ 163

  Fly  1088 -----------PSSADAAILTNTSLIFR-----DLDQACMEAVAALL----RGLPLQPEESDRGD 1132
                       ||.::|    ..:.:.|     ..|:.....|...|    |...::.::.|:..
Zfish   164 CDPYAAVSLLGPSRSEA----KKTKVKRKNNNPQFDEIFFFEVTKPLSFTKRQFDVEEDDVDKLA 224

  Fly  1133 L-MDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNNTPL--------------LPPRPR----MA 1178
            | :|..:|..||:...|:..:              ..||              |.||..    :.
Zfish   225 LKVDLWNASNLKFGDEFLGEV--------------RVPLKVLGQAGVHDAWYFLQPRDNGHKSVK 275

  Fly  1179 AGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNP--DLQTRAAFMEVLTQILQQGTEFDTLA 1241
            |.:|.:||...:....::....      |     |||  ||..::|.::
Zfish   276 ADELGSLRLNIVYTEDHVFPTE------H-----YNPLRDLLLQSAHVQ---------------- 313

  Fly  1242 ETVLADRFEQLVQLVTMISDKGELPIAMALANVV--TTSQMDELARVLVTLFDAKHLLSPLLWNM 1304
                                    |::.:.|:::  ...:..|.|..||.||.....:.|.|..:
Zfish   314 ------------------------PVSASAAHILGEVCREKQEAAVPLVRLFLHYGKIVPFLSAI 354

  Fly  1305 FYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLLDE---EEETCFEVDPA 1366
            .:.|:..:....|:||||||.||.:....|:.|..||::.|:|:|    ||   :.:.| |:||.
Zfish   355 AHAEISRTQDPNTIFRGNSLTSKCIDETMKLAGMHYLRVTLKPII----DEICSDHKPC-EIDPV 414

  Fly  1367 RLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGT 1431
            :|..:|:::.:|.||.....::|..|.:|....|..:..:...|.:..:.||.........||.:
Zfish   415 KLKESENLDTNRENLRQYVDRIFSVITSSGVSCPTVMCDIFFSLRESAASRFQVDADVRYTAVSS 479

  Fly  1432 VIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVEFS----KEQHMLCFNDFL 1492
            .|||||..|||:||....:..........|.|.|:||.:|.:.:..:..    ||.:|..|.|:.
Zfish   480 FIFLRFFAPAILSPNLFHLRPHHPDPCTSRTLTLISKTIQTLGSLAKSKSANFKESYMAAFYDYF 544

  Fly  1493 RDH-FEAGRRFFIQIASDCETVDQTS 1517
            .:. :....:.|:.:.|.....||.|
Zfish   545 NEQKYADAVKNFLDLISSSARWDQKS 570

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nf1	NP_733132.2	RasGAP_Neurofibromin	1242..1575	CDD:213332	73/286 (26%)
		CRAL_TRIO_2	1633..1767	CDD:404584
		PH_NF1	1759..1868	CDD:270123
		MOR2-PAG1_mid	<1931..>2109	CDD:372971
rasa3	XP_009303503.1	C2A_RasA2_RasA3	13..133	CDD:176046	25/153 (16%)
		C2B_RasA3	146..287	CDD:175977	25/158 (16%)
		RasGAP	278..629	CDD:214617	83/349 (24%)
		RasGAP	296..563	CDD:295371	77/322 (24%)
		PH-like	561..705	CDD:302622	4/10 (40%)
		PH	597..695	CDD:278594
		BTK	698..733	CDD:128417
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R472
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.940