DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Lnk and SH2B1

DIOPT Version :9

Sequence 1:	NP_001287536.1	Gene:	Lnk / 43130	FlyBaseID:	FBgn0028717	Length:	728	Species:	Drosophila melanogaster
Sequence 2:	NP_001139267.1	Gene:	SH2B1 / 25970	HGNCID:	30417	Length:	756	Species:	Homo sapiens

Alignment Length:	883	Identity:	251/883 - (28%)
Similarity:	347/883 - (39%)	Gaps:	316/883 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    31 GTSSAAAAAAAGSDLIPAPIGTGNAMGVSSYAYGGTSWEEFCERHARVAASDFAKACITYINGNL 95
            |..|....|:..|..:|.|              ...||.||||.|||.||.|||:....|:..:
Human     3 GAPSPEDGASPSSPPLPPP--------------PPPSWREFCESHARAAALDFARRFRLYLASH- 52

  Fly    96 PPEEARNIQHRSFAQKFVESFSAHYDTEFFKRRSTLKSGAGSLDFEEEHEVPKLL------SKSL 154
             |:.|......:|:::|.|.|..|::.|       :...:|||.       |.:|      ::..
Human    53 -PQYAGPGAEAAFSRRFAELFLQHFEAE-------VARASGSLS-------PPILAPLSPGAEIS 102

  Fly   155 LRRLSFKGLR-KGKISLLQAFFHKNSDDLDG------SGGSGKQSKTKLAKI------------- 199
            ...||.:..| .|.:::|..  .::|:||.|      |..|...||.||.|.
Human   103 PHDLSLESCRVGGPLAVLGP--SRSSEDLAGPLPSSVSSSSTTSSKPKLKKRFSLRSVGRSVRGS 165

  Fly   200 ---VVECRKEGTV---------------------------------------NNLTP-------- 214
               :::.|  |||                                       :..:|
Human   166 VRGILQWR--GTVDPPSSAGPLETSSGPPVLGGNSNSNSSGGAGTVGRGLVSDGTSPGERWTHRF 228

  Fly   215 -------------------------------ESLDQPT----------------GSQKWEKCRLV 232
                                           |:...|.                |..:|:||||:
Human   229 ERLRLSRGGGALKDGAGMVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSGGGGQPQWQKCRLL 293

  Fly   233 L---VKAVGGYMLEFYTPHKATKPRSGVFCFLISEARETTALEMPDRLNTFVLKADNNMEYVIEA 294
            |   .:..||..|||:.|.||::||..:.|..|::.|.|||||||||.||||:|.:...||::|.
Human   294 LRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEGPSEYIMET 358

  Fly   295 ESAEEMRSWLATIRYCMR----------------TPPTQQPTIE-SDGVMASAMQTSPTLPSPNP 342
            ..|:.:::|::.|:.|:.                .|.|...|.| :|.:..|.:..|.:|||.:.
Human   359 VDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPSQDL 423

  Fly   343 IGGIQNPQYQQQRGSNGNLVGGGAPLTSSLSADSALGQGGATSASELNVINELGTSPTSGPPDIP 407
            :.|   |.....|.|.|...|.....::|:|..||     :.:||..:.:..|   |...||.||
Human   424 LLG---PSESNDRLSQGAYGGLSDRPSASISPSSA-----SIAASHFDSMELL---PPELPPRIP 477

  Fly   408 VR--PHRG--------------EQRLSASSNFDG-IEGTENDADVADLTAEMSVFPWFHGTLTRS 455
            :.  |..|              .:..:.|..|.| .||.|.|       ..:|.:|||||.|:|.
Human   478 IEEGPPTGTVHPLSAPYPPLDTPETATGSFLFQGEPEGGEGD-------QPLSGYPWFHGMLSRL 535

  Fly   456 EAARMVLHSDAAGHGYFLVRQSETRRGEFVLTFNFQGRAKHLRLTISEKGQCRVQHLWFPSIQEM 520
            :||::||......||.||||||||||||:||||||||:||||||:::|:||||||||||.||.:|
Human   536 KAAQLVLTGGTGSHGVFLVRQSETRRGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDM 600

  Fly   521 LEHFRHNPIPLESGGTSDVTLTEWVHSHSRLNDPTTAANHDSGQ------LNDLSTNGNGNGNGN 579
            |||||.:||||||||:|||.|..:|.|..|..:|||  :||..|      ..|....|
Human   601 LEHFRVHPIPLESGGSSDVVLVSYVPSSQRQQEPTT--SHDPPQPPEPPSWTDPPQPG------- 656

  Fly   580 GYDNGQGSSTASNAAGGTASGA--------AGGGHPSPRHCNEVITMNLSVRLKTNEIELPQEPT 636
                .:.:|.|...|...|:.|        ||||                        .:|:|
Human   657 ----AEEASRAPEVAAAAAAAAKERQEKEKAGGG------------------------GVPEE-- 691

  Fly   637 HVYFPEQVYFHLDPTTLTVHGSPPPAQNFLDQPHLRASNASLQAAAHHQAGSSGNRHPSDGGSNS 701
                       |.|....|             |.:....|....:....|||.|:
Human   692 -----------LVPVVELV-------------PVVELEEAIAPGSEAQGAGSGGD---------- 722

  Fly   702 GGAG------------GGSGSSGGAECTGRAVDNQYSF 727
              ||            ||.|..||..   ||::|||||
Human   723 --AGVPPMVQLQQSPLGGDGEEGGHP---RAINNQYSF 755

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Lnk	NP_001287536.1	Phe_ZIP	67..125	CDD:286060	23/57 (40%)
		PH_SH2B_family	202..313	CDD:269938	48/223 (22%)
		PH	220..312	CDD:278594	42/110 (38%)
		SH2_SH2B_family	438..534	CDD:198209	64/95 (67%)
SH2B1	NP_001139267.1	Interaction with JAK2 (low-affinity binding, independent of JAK2 phosphorylation). /evidence=ECO:0000250	1..555		151/603 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..27		7/37 (19%)
		Required for self-association	24..85		23/69 (33%)
		Phe_ZIP	26..81	CDD:401022	22/63 (35%)
		Interaction with RAC1. /evidence=ECO:0000250	85..196		25/121 (21%)
		Required for NGF signaling. /evidence=ECO:0000250	100..243		21/146 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	123..154		11/30 (37%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	169..222		5/54 (9%)
		Required for nuclear localization. /evidence=ECO:0000250	224..233		0/8 (0%)
		PH_SH2B_family	246..378	CDD:269938	43/131 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	263..286		2/22 (9%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	420..455		9/37 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	468..491		8/25 (32%)
		SH2_SH2B_family	518..614	CDD:198209	65/102 (64%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	626..688		19/98 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	709..756		18/62 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159494
Domainoid	1	1.000	124	1.000	Domainoid score	I5520
eggNOG	1	0.900	-	-		E1_2CN0G
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	296	1.000	Inparanoid score	I2744
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D255086at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		oto90655
orthoMCL	1	0.900	-	-		OOG6_107031
Panther	1	1.100	-	-	LDO	PTHR10872
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3118
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.760