Protein Alignment Clbn and RQC2

DIOPT Version :9

• Sequence 1: NP_001163721.1
• Gene: Clbn / 43018
• FlyBaseID: FBgn0259152
• Length: 992
• Species: Drosophila melanogaster
• Sequence 2: NP_015316.1
• Gene: RQC2 / 856098
• SGDID: S000005930
• Length: 1038
• Species: Saccharomyces cerevisiae
• Alignment Length: 1086
• Identity: 283/1086 - (26%)
• Similarity: 485/1086 - (44%)
• Gaps: 195/1086 - (17%)

  Fly     1 MKTRFNTFDIICGVAEL-QKLVGWRVNQIYDVDNKTYLFRMQGTGAVEKVTLLIESGTRFHTTRF 64
            ||.|.:..|::....|| |.|.|:|::.||::.:.:..|.::......|:.::::.|.|.:.|.|
Yeast     1 MKQRISALDLLLLARELKQDLEGYRLSNIYNIADSSKQFLLKFNKPDSKLNVVVDCGLRIYLTEF 65

  Fly    65 EWPKNMAPSGFSMKLRKHLKNKRLEKVQQMGSDRIVDFQFGTGDAAYHVILELYDRGNVILTDYE 129
            ..|....||||.:|||||||.|||..::|:..|||:..||  .|..::::||.:..|||||.|
Yeast    66 SRPIPPTPSGFVVKLRKHLKAKRLTALKQVDQDRILVLQF--ADGHFYLVLEFFSAGNVILLD-- 126

  Fly   130 LTTLYILRPHTEGENLRFAMREKYPVERAKQPTKELEL----------EALVKLLENARNGDYLR 184
                         ||.|....::..:|...:..:..|:          |:..:.:|..|..:|..
Yeast   127 -------------ENRRIMALQRVVLEHENKVGQIYEMFDESLFTTNNESADESIEKNRKAEYTS 178

  Fly   185 QILTPNLDCGPAVIEHVLLSHGLDNHVIKKETTEETPEAEDKPEKGGKKQRKK------------ 237
            :::.       ..|:.|...:..|..|||        :...:.::|.||::.|
Yeast   179 ELVN-------EWIKAVQAKYESDITVIK--------QLNIQGKEGAKKKKVKVPSIHKLLLSKV 228

  Fly   238 --------QQNTKL-----EQKPFDMVNDLPILQQAVKDAQ----ELIAEGNSGKSKGYIIQVKE 285
                    .:|.|:     .:...:::.:...|.:.:...|    :|:   .:...||||:..:.
Yeast   229 PHLSSDLLSKNLKVFNIDPSESCLNLLEETDSLAELLNSTQLEYNQLL---TTTDRKGYILAKRN 290

  Fly   286 EK-PTENGTVEFFFRNIEFHPYL-FIQFKNFEKATF----ESFMEAVDEFYSTQESQKIDMKTLQ 344
            |. .:|..|.:..|....|||:. :|...:.:.:..    ..:...:|:|:||.||.|..::...
Yeast   291 ENYISEKDTADLEFIYDTFHPFKPYINGGDTDSSCIIEVEGPYNRTLDKFFSTIESSKYALRIQN 355

  Fly   345 QEREALKKLSNVKNDHAKRLEELTKVQDVDRKKAELITSNQSLVDNAIRAVQSAIASQLSWPDIH 409
            ||.:|.||:.:.:.::.::::.|..||:::.:|..||..|..|::....|||..|..|:.|..|.
Yeast   356 QESQAQKKIDDARAENDRKIQALLDVQELNERKGHLIIENAPLIEEVKLAVQGLIDQQMDWNTIE 420

  Fly   410 ELVKEAQANGDAVASSIK-QLKLETNHISLML---------SDPYDND---------EDDDLKDP 455
            :|:|..|..|:.:|..:. .|.|:.|.||:.|         |...||:         .|.|.:|.
Yeast   421 KLIKSEQKKGNRIAQLLNLPLNLKQNKISVKLDLSSKELNTSSDEDNESEGNTTDSSSDSDSEDM 485

  Fly   456 EVTV----------------VDVDLALSAWANARRYYDMKRSAAQKEKKTVDASQKALKSAERKT 504
            |.:.                |.:||.|||:|||..|:::|:::|||:||......||:|:.|.|.
Yeast   486 ESSKERSTKSMKRKSNEKINVTIDLGLSAYANATEYFNIKKTSAQKQKKVEKNVGKAMKNIEVKI 550

  Fly   505 QQTLKEVRTISNIV--KARKVFWFEKFYWFISSENYLVIGGRDAQQNELIVKRYMRPKDIYVHAE 567
            .|.||:....|:.|  |.|..::|||:.||||||.:||:.|:...:.:.|..:|:...|||:...
Yeast   551 DQQLKKKLKDSHSVLKKIRTPYFFEKYSWFISSEGFLVMMGKSPAETDQIYSKYIEDDDIYMSNS 615

  Fly   568 IQGASSVIIQNPTGEEIPPKTLLEAGSMAISYSVAWDAKVVTNSYWVTSDQVSK-TAPTGEYLAT 631
            ..  |.|.|:||...|:||.||::||.:.:|.|.||..|:.::.:|..:..||| .......|..
Yeast   616 FN--SHVWIKNPEKTEVPPNTLMQAGILCMSSSEAWSKKISSSPWWCFAKNVSKFDGSDNSILPE 678

  Fly   632 GSFMIRGK--KNFLPSCHLTMGLSLLFKL----------EDSFIERHLGERKVRSLEDDQIDPNV 684
            |:|.::.:  :|.||...|.||...|:|:          :|...|....|.:....|:::.:...
Yeast   679 GAFRLKNENDQNHLPPAQLVMGFGFLWKVKTSGNEDNGDDDEEEEEEEEEEEEEEEEEEEEEEEE 743

  Fly   685 KENEVEHDLLSDNEDADSNINLSEPSSNTEITAFPNTEVKIEHDTGRIIVRSDSVNPEIEETKES 749
            ||.|.:.:....:||..:.:|..|...::..:...|:   .|||.
Yeast   744 KEEEEKEEEQQQDEDDSNEVNGLEKGGDSNDSTKNNS---FEHDN-------------------- 785

  Fly   750 EVVLDKILKKTDDEETTIILAGPSRKKQVSAKKTKEDKARAKQEAAKQEVPPVSSEP-------K 807
                   |:|..::..||            :..|..|...||.:........:..||       :
Yeast   786 -------LEKDIEKHCTI------------SSDTDSDSGNAKAKNDNSSTQRILDEPGVPISLIE 831

  Fly   808 NPSQVKRGQKGKLKKMKQKYKDQDDEEREIRMMILKS-SGKEKPQASADKVVEKSESTKEYVKPE 871
            |.:...||::|||||:::||.|||:.||.:|:..|.: .|.||.|....:.:.|.|..::.....
Yeast   832 NINSNVRGKRGKLKKIQKKYADQDETERLLRLEALGTLKGIEKQQQRKKEEIMKREVREDRKNKR 896

  Fly   872 KSAAPKNPVELDDADEVPVGGDVDVLNSLTGQPHEGDELLFAIPVVAPYQALQNYKFKVKLTPGT 936
            :.......::....::..|..|.. .:.|.....:||.:...|||.||:.||..||:|||:.||:
Yeast   897 EKQRRLQALKFTKKEKARVNYDKH-KSELKPSLDKGDVVDDIIPVFAPWPALLKYKYKVKIQPGS 960

  Fly   937 GKRGKAAKLALNIFAKEKSCSA----------REKDLLKSIKEESL 972
            .|:.|.....|:.| |.:....          :|.:::|.:||:.|
Yeast   961 AKKTKTLTEILHYF-KSRPLDGSSTDNEMDWPQEHEMIKGLKEQDL 1005

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Clbn	NP_001163721.1	YloA	1..643	CDD:224212	199/727 (27%)
		DUF814	531..630	CDD:283353	35/99 (35%)
		DUF3441	883..984	CDD:288752	29/100 (29%)
RQC2	NP_015316.1	YloA	1..699	CDD:224212	202/734 (28%)
		DUF3441	915..1017	CDD:403211	29/93 (31%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157343603
Domainoid	1	1.000	169	1.000	Domainoid score	I790
eggNOG	1	0.900	-	-		E1_COG1293
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	329	1.000	Inparanoid score	I433
Isobase	1	0.950	-	0	Normalized mean entropy	S2364
OMA	1	1.010	-	-		QHG54163
OrthoFinder	1	1.000	-	-		FOG0005262
OrthoInspector	1	1.000	-	-		oto99336
orthoMCL	1	0.900	-	-		OOG6_101297
Panther	1	1.100	-	-	LDO	PTHR15239
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1916
SonicParanoid	1	1.000	-	-		X4285
TreeFam	1	0.960	-	-
	15	14.740