Protein Alignment AFDN and cno

DIOPT Version :10

• Sequence 1: XP_047274744.1
• Gene: AFDN / 4301
• HGNCID: 7137
• Length: 1909
• Species: Homo sapiens
• Sequence 2: NP_524232.2
• Gene: cno / 40620
• FlyBaseID: FBgn0259212
• Length: 2051
• Species: Drosophila melanogaster
• Alignment Length: 2193
• Identity: 726/2193 - (33%)
• Similarity: 1046/2193 - (47%)
• Gaps: 451/2193 - (20%)

Human     9 ERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIE 73
            :|..:..:|..|||||||||.:|:|.|:|.||||||||||| |....|||||||:|.||..|||:
  Fly     9 DREAVRSVIQQWNANRLDLFALSEPDENLLFHGVMRFYFQD-AGQKVATKCIRVASDATVTDVID 72

Human    74 TLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPP 138
            ||.|||||||||||.|.|:|||||.:||||||:.|||||:|||||:.|||||||:|||.:
  Fly    73 TLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLKNID----- 132

Human   139 KKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASD-------------------- 183
               |...|.:|..    .||||.|||:||||:||:||.| :.:||
  Fly   133 ---QKTTPIEQTD----LNFKRKLSKREKKEQKKKEKMA-KLSSDPPTSNGSHLGLGNGVGASSG 189

Human   184 ----KDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGR 244
                ..:....|.| .:..:|.::|.::|||||||:|||||.||:|||||||||::|:|||.||.
  Fly   190 SAHMNGNAGGTGGD-SSDAVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGG 253

Human   245 PDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQH 309
            ||:||||:||.:||..::||||:|||..|.|...|.|.|.||||||.:|..||:.:|       :
  Fly   254 PDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-------N 311

Human   310 SDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGS 374
            ||   ..|.|||||||||.|....|:.:|.::|.::|||.|..|::.||...| ...|.....|.
  Fly   312 SD---GTEYILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKKKPLG-AANGANHISGD 372

Human   375 GYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSI 439
            ..|        |.|||::            |:.:.|.         |::|....|.....:.|.:
  Fly   373 REG--------PVLVEVT------------HSGDGGR---------RIKLGSDPVEVGSANTNCL 408

Human   440 QLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPS 504
            |||||.|||.||.::.::||.||||...||.|:|.|..||:.|:|.:|..|.||....::|:|..
  Fly   409 QLFGPSIQPRHCLISLLEGVCTVTPLHTDALTFVNGHHISQPTILHNGSVVMFGRVASYRFLDSP 473

Human   505 QDH----ALAKRSVDGGLMV--KGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSS 563
            .|.    ||::..:|...:.  :.|. .||...:         ..|....|.||    |.|....
  Fly   474 TDGRYNLALSQSQLDSACLYESRSPT-SPGSWND---------EDGALSSTHKS----DYDHHQQ 524

Human   564 AS-----------------STAERGMVKPMIRVEQ---QPDYRRQE------------------- 589
            |:                 |:..|.:|......:|   ..:|..|.
  Fly   525 ANHSSAYHNNNNNSSDHPLSSTLRDVVDSKAGQDQGANSGNYDGQSLEGNLENETKSISSLKSGG 589

Human   590 SRTQDAS--------------------GPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLS 634
            |.:||.|                    |.|.||||.:||.|:.::.||..||:..:.:..||||:
  Fly   590 SNSQDRSPKMTGSGILAGGGSGSGDTQGQEPILPAVLEFPETHQELFLRHIISELDVNVPHFKLA 654

Human   635 PTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFW 699
            |.|.||:..||..|..|||::.||||.||:...::.:.:::..|:||       |......||||
  Fly   655 PVYSLYLCARYRASTHYRPELQPTERAHKLTMFLHHVANLVYSVVQE-------QYTDPRILAFW 712

Human   700 MANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQR 764
            |||:||.|:|:|.||.:|..::.||:|||..||.||:.||:|.:.||:..:..||.:
  Fly   713 MANSSEFLHFLKSDRHISAFSVQAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSE-------- 769

Human   765 PKIDD------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYW 823
             .||.      ||..|..||:|||||||||||||||||||||:||:..||.:|.  :|.:|:..|
  Fly   770 -NIDHDSAAGLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVA--NSQMCTGDW 831

Human   824 GAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQA 888
            |.::.::|..:|.|||:|||||||||||::|.|....|...|.:.::|..:..:||:|||||:.|
  Fly   832 GKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAA 896

Human   889 LLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVV 953
            |||       :..:|.:|::..:.:||:.||||.|:|||||:|||.|:|.|..|||:||:|||||
  Fly   897 LLQ-------QEKLPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVV 954

Human   954 RNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITV 1018
            |.||.||.:||:||.|:|.|||.....|.|.||:|....:..|.....| :|.||   |.::|.:
  Fly   955 RGIPTGLVDFLNPLQQQGMCRLAAQPTSIGLWTVYMHQFNARSSSAMSN-KLPQP---ELQLIKL 1015

Human  1019 TLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAA 1083
            . |..|||||||||||||||:|||||:||||.|||||.||||.||||||.|||:||:|::|||||
  Fly  1016 H-KNSNGMGLSIVAAKGAGQEKLGIYIKSVVPGGAADADGRLQAGDQLLRVDGQSLIGITQERAA 1079

Human  1084 ELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQ----RISDR---RGSGKPRPKSEGFELYN 1141
            :.:.||..||:|||||||||||||||||.||||::|    |:|:|   |..|....||.|..:.|
  Fly  1080 DYLVRTGPVVSLEVAKQGAIYHGLATLLQQPSPVIQRGNRRMSERDLTRMGGAESTKSLGPLIAN 1144

Human  1142 N---------------------STQNGS---PESPQLPWAEYSEPKKLP------GDDRLMKNRA 1176
            |                     ::.|.|   |.:..||     ..|.:|      |...:....:
  Fly  1145 NHPNHSLSLSQHLNNSHNNTLGNSNNSSIINPLAAHLP-----NSKSVPALHHHTGSGTISLANS 1204

Human  1177 DHRSSPNVANQPPSPGGKSAYASG------TTAKITSVSTGN------------LCTEEQTPPPR 1223
            ..||:.::.|.....||....|:|      ..::..:...||            ...:.|:.|..
  Fly  1205 KSRSTHSLHNNTSGMGGIGGAAAGGMLGQPNGSQNNANGNGNGNEQGFYQNLSVYRAQNQSQPIL 1269

Human  1224 PEAYPIPTQTYTREY-FTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSN-----------HSSIA 1276
            .|..||........| .:.|.:..|......|:.:...:::.||..::|           ||..:
  Fly  1270 NERPPIAAHAAMNAYNGSSPLAPQQQPQQQQQSPYQQQQQQQHMQANANLPPTRPVSAYYHSQQS 1334

Human  1277 IQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSS---SLDSSTSSQEHLNHSSKSVT 1338
            .|:..:.|::.::..:.|.::..:.:............:|..   :|::.|.||::..|:
  Fly  1335 AQQQLQQQQQQQQQHSLQQQQFALSSGNLNGQQQQQQQHQHQHQLTLNNRTKSQQNFQHT----- 1394

Human  1339 PASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPV----HYAGDFDGMSMDLPLPPP 1399
                                         :|......|..|.:    |:.   ......|||...
  Fly  1395 -----------------------------LRMQQMMAPSMPNISNMYHHQ---QQQQQQLPLQQQ 1427

Human  1400 PSANQIGLPSA--------------QVAAAERRKREEH--QRWYEKEKARLEEERERKRREQERK 1448
            ....|..|.|:              |..:.|.|:.:.|  ...||.::.:|::::::::::|:::
  Fly  1428 QQQQQQPLMSSSQSMQNVNDFAGGYQNGSLEYRRSQLHDPSTLYEIQQQQLQQQQQQQQQQQQQQ 1492

Human  1449 LGQMRTQSLNPAPFS-----------PLTA---QQMKPEKPSTLQRPQETVIRELQPQQQ--PRT 1497
            .......:|.|.|..           |.||   ||.||..|.|...|.....:::.|...  ..|
  Fly  1493 QASPNFIALPPKPLGSLQSPNKPNVPPSTAPKPQQDKPPLPPTATHPLFKATQQIAPGMNYVAST 1557

Human  1498 IERRDLQYITVSK---EELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSA 1559
            ::.....|:..::   ..|.||.    :||:|:.:||..:.::.||.....::|.||.....||.
  Fly  1558 LDPPKGSYVASNQGNNRPLHSGS----NPWEREEREKDLEMRREHIRQWREQQISELSQIVSRSP 1618

Human  1560 EESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQ---DEERRRQ 1621
            .:.::|:.|:||..|::|.||.::::|.|:|::.|..:...:.:.....:.:.:|   ...|:.:
  Fly  1619 MQEEQLKTLILERDFERRAQELQEQEEQDQEQQYDKENVQELFRLAGGGQVSAIQTPITSYRQTE 1683

Human  1622 QQLEEMRKREA----------EDRARQEEERRRQEEERTKRDAEEKRRQEEG------------- 1663
            .:|.||....:          ...|:......:|.:...|.:     |..||
  Fly  1684 IKLAEMPDSNSLVDSVPPQPPAPTAQPLSSNTQQPKSILKHN-----RYSEGGVGPSGAPSSPSK 1743

Human  1664 -YYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYL--KT 1725
             ..|...|:.|..|.|.....|..|...|..  |.:|....: ..|...|||||:||:|||  ..
  Fly  1744 SQKSASFADERHLHTEHPISNLAKELNQLTM--LDKDNNNET-LDAVVPPPPPPERNSSYLIMSQ 1805

Human  1726 QVL------SPDSLFTAKFVAYNEEE---EEEDCSLAGQDKYSSTRKS--------HGDLLPA-- 1771
            |.|      :..|:...|....|:..   |.:..||......::...|        ||..|.|
  Fly  1806 QKLRGSTGNATSSMGLLKTATSNQAAAAVEIKKASLLNTQTNNNNNLSGSLNNNTMHGSPLSAME 1870

Human  1772 -----------------PLKPRPPPCQPRP-------ASDGVFLSNSFQPPSAKANSTAHKKG-- 1810
                             ...|.|||...|.       ..:..|:|.:.|....:........|
  Fly  1871 LNAAYVSATGTGIIGGLGTPPPPPPLMQRDNKRVSFHDEENNFVSGNSQQQQLQQQYIMDNYGME 1935

Human  1811 -QPLPPPKKSSSYHPSHCKGRGPNSYPG------------STGAAVGAHDACRDAKEKR---SKS 1859
             |.|...::.:||...:.......|.|.            ||...:||.:..||.:.:|   .:.
  Fly  1936 HQDLDTIREDTSYLQHNLDASMLPSMPATPDAALWNTSVQSTPGVIGAQEVYRDPRTRRLAEKQQ 2000

Human  1860 QDADSPGSSGAPENLTFKERQRLFS--QGQDVS--NKVKASRKLTELE 1903
            |..........||.|:|||:.::|:  .|:|.:  :|:|.||...:::
  Fly  2001 QQQQQRAGDAVPEKLSFKEKMKMFALESGEDKTPKDKLKISRAQRDID 2048

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AFDN	XP_047274744.1	RA1_Afadin	20..132	CDD:340480	83/111 (75%)
		RA2_Afadin	248..348	CDD:340479	47/99 (47%)
		FHA_AFDN	385..503	CDD:438763	40/117 (34%)
		Myo5p-like_CBD_afadin	612..940	CDD:271255	147/333 (44%)
		PDZ_AFDN-like	1013..1100	CDD:467251	60/86 (70%)
		DUF4670	<1470..1688	CDD:464815	52/249 (21%)
cno	NP_524232.2	RA1_Afadin	20..130	CDD:340480	82/110 (75%)
		RA2_Afadin	257..347	CDD:340479	47/99 (47%)
		FHA	376..471	CDD:469597	40/115 (35%)
		Myo5p-like_CBD_afadin	631..941	CDD:271255	147/334 (44%)
		PDZ_AFDN-like	1008..1096	CDD:467251	62/91 (68%)