Protein Alignment AFDN and afd-1

DIOPT Version :10

• Sequence 1: XP_047274744.1
• Gene: AFDN / 4301
• HGNCID: 7137
• Length: 1909
• Species: Homo sapiens
• Sequence 2: NP_001021660.2
• Gene: afd-1 / 171776
• WormBaseID: WBGene00021009
• Length: 1660
• Species: Caenorhabditis elegans
• Alignment Length: 1874
• Identity: 537/1874 - (28%)
• Similarity: 818/1874 - (43%)
• Gaps: 410/1874 - (21%)

Human     9 ERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIE 73
            ||.:||.::..||.||||||.:|.||||||..||||||||| ......|||:||||||||:.|::
 Worm     4 EREQLACLVQQWNENRLDLFHLSYPTEDLEVEGVMRFYFQD-GGEKVLTKCVRVSSTATTRAVVD 67

Human    74 TLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPP 138
            .|:|||.||::|||:..|||:|||.:||||:|..:||||||||.|:||||||||:||  .||.||
 Worm    68 ALSEKFLPDLKMLSNDTYSLWEVHENGEERKLLDEEKPLVVQLQWHKDDREGRFLLK--KDAAPP 130

Human   139 KKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEV 203
            ....:    .||.:   |...|...||:|:.|||.:|..:....:......|.:|        ::
 Worm   131 VSLLN----LQEAD---QKNTRFAKKKDKEPKKKNQKNFVSVQVNGGREEAQSQD--------DI 180

Human   204 YKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTIL 268
            ||.:|..:|||||||||.||::||::|||.:::|..       .||:|::|...:.|..||.:||
 Worm   181 YKQVPFNTFTRTISNPEAVMRKRREKKLETKLKEMM-------FGGSLKVYGGEIVPTRPYVSIL 238

Human   269 LSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEI------ILDDDECPL 327
            ....:.||..:..|||||||| .:..|:.:..|     :...|.|...::      .:...||||
 Worm   239 AEINENADRILGAALEKYGLE-HSKDDFILVEV-----SNDDDRKSMSDLREIDGRPIPPTECPL 297

Human   328 --QIFREWPSDKGILVF-QLKRRPPDHIPKKTKKHLEGKTPKGKERADGS-----GYGSTLPPE- 383
              ...|....:.|...| .:||:|.|:           :.|.....|..|     ...::|||:
 Worm   298 FEMTARSGNGENGFDSFLAIKRKPHDY-----------QVPSTSVVAPSSVTLPMSPLTSLPPQI 351

Human   384 -KLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTE----KLDDNSIQLFG 443
             .||.|:.::               .||..|..:..|:.   .|||||::    ....::|.|.|
 Worm   352 HNLPVLIVMN---------------NDGHLSTHRITLHE---GVTEVGSDSQMSNFSQHNIYLDG 398

Human   444 PGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKF-VDPSQDH 507
            |.|:..|..:..|:||||:||.:.||...|.|.::.:|.:|:.|..::.||.|.||| ...||.:
 Worm   399 PDIRGRHAAIAFMEGVVTLTPSTRDAYLEVNGHQLMQTEILRDGDHIRIGADHSFKFSTSSSQAN 463

Human   508 ALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTA----------------LPTSKSTTRL 556
            .:...::..    ...||   ..|:|.:....|..:.||                .|.|.|.:.:
 Worm   464 QINSTNISN----SNQRH---FQQQTFYPEMADSRASTARCAPSDYCSLPQQNDYYPPSMSGSSV 521

Human   557 DSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAII 621
            .|.|.......:...::.|.              .|:.|..| |.||.||:..:.:...||:.::
 Worm   522 SSSRQEDLMMHSNNNILPPY--------------STKSAQEP-LQLPLSIQIGDYNVWDFLAEVM 571

Human   622 N---YTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVD 683
            :   .|:|....|:|||.|.||:|.|             :..||      ||..|     :|...
 Worm   572 SNRAATSSEMSTFRLSPAYSLYLALR-------------SFHTH------NKPYS-----VQFFS 612

Human   684 QVDQKQKNIA------GALAFWMANASELLNFIKQDRDL-----SRITLDAQDVLAHLVQMAFKY 737
            :::...|.||      ..|.||:|||||..:.:::|.:|     .:|:::        ::..|:.
 Worm   613 RIESNFKQIAQECSSKDDLIFWLANASEFNHLVERDTELKASRYGKISVE--------IERIFRR 669

Human   738 LVHCLQSELNNYMPAFLD----DPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQL 798
            ||..|||.:.....|.||    |.:...       ||:|.|...|...|.|.:||||||||...|
 Worm   670 LVAVLQSVIRPAARALLDIHLNDHDATG-------DVIHLLDTTMRQARICGLNAALTIQLCGHL 727

Human   799 FHFINMWLFNRLVTD--PDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLL 861
            .|.:|.::||.||:.  |.:.|.:.. |..::.::..|..:.|:.|:|:||:|||.|..||..||
 Worm   728 LHMVNAFVFNSLVSVNLPAAQLNTRL-GKCLQYRIESIHRFCERMGVEMAAECHLDRSRQAANLL 791

Human   862 TMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIP--TDLIENVVTVAENTADELARS 924
            ...|   :|:..:.|||:||||||:..:|..:.  ||:..||  .|:::.:..:||..||:|...
 Worm   792 ATQK---NDVAALGSTCYKLNSLQVVHILSGFE--PDDGEIPCDDDVVDRICRLAEKQADKLTLE 851

Human   925 DGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSP------- 982
            ||:.|.|||..:|.||||:|:|||..:..|.:|:||.::|..|..|..|..:..|.:|
 Worm   852 DGQRVTLEESDELNLPFLIPQDGYFVEQFRTVPDGLWQYLQQLQHRRLCHTVSLTPAPPAAMTHS 916

Human   983 -GTWTIYFEGADYESHLLRENTELAQPLRK-----------------------EPEIITVTLKKQ 1023
             ....|....|.|..|   ::.:...|:.:                       ...|..|||:|.
 Worm   917 MHAMHIGASDAHYHQH---QHQQQQNPIVRSVSQTTLTSIPTTQFGDSFGSAVSDTISKVTLRKN 978

Human  1024 -NGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMT 1087
             .|:|||||||:|.|..::|||||.||:|..|..||||..|||||||:|.||:|:|||.||.|||
 Worm   979 GGGIGLSIVAAQGVGDRQMGIYVKKVVEGTPAAHDGRLETGDQLLSVNGHSLIGISQEDAARLMT 1043

Human  1088 RTSSVVTLEVAKQGAIYHGLATLL---------------------------------NQPSPMM- 1118
            ::.:.|..:|.|:.|..:||:..|                                 .||.|..
 Worm  1044 QSGNEVHFDVRKRAAHRNGLSNWLGSQTPTTSNFIAPSPSTVPPPSYSHVGRTPSYDGQPPPSYN 1108

Human  1119 QRISDRRGSGKPRPKSEGFEL-YNNSTQNGSPESPQLPWAEY---------SEPKKLPGDDRLMK 1173
            .|.....|:|....::..|.. .:||:.|.|....|..:|.:         :.|....||.:..:
 Worm  1109 HRNGGASGAGGSSGRNSAFVAPLSNSSSNASSSHMQQHYAHHNMMHNGNLSAAPGAPAGDPKHYR 1173

Human  1174 --NRADHRSSPNVANQPPSPGGK----------SAYASGTTAKITSVSTGNLCTEEQTPPP-RPE 1225
              :.:|.:..||.:....|..|.          |.|...|...:...:.    ...|:|.. |..
 Worm  1174 SSSASDLQHDPNASFSQSSMTGSARSSVFDKLPSHYRHSTRPTVIQPTR----PSAQSPSALRKA 1234

Human  1226 AYPIPTQTYTREYFTFPASKSQDRMAPPQNQ--WPNYEEKPHMHTDSNHSSIAIQRVTRSQEELR 1288
            |...|.....|     |.|.| :..|||:|.  ...|        .||.:..:.|.:.|:..:|.
 Worm  1235 AAASPVSNLYR-----PTSAS-NLFAPPRNSGGLVGY--------SSNAARESTQDIHRALNDLE 1285

Human  1289 ---------EDKAYQLERHRIEAAMDRKSD---------SDMWINQSSSLDSSTS---SQEHLNH 1332
                     .::.|::...|:.......|:         :.|:..||.|  |||:   :.:.:..
 Worm  1286 LNSPLARSTPNEEYRIPPPRVPTVTSTSSNYAPLHSIRPASMYGYQSPS--SSTTVIPAPKGVAS 1348

Human  1333 SSKSVTPASTLTKSGP-GRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPL 1396
            ||.:.:|...:|..|. ...::..::...|..|.:|:..::|...||..:..|.. ..::..|.|
 Worm  1349 SSNNTSPRVNVTLGGALSSSRSHNSLSQEPAIVIRPVDLNMPKATPPASIRGAVP-TAITQALQL 1412

Human  1397 PPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAP 1461
            .|    |..|....||..|...                   |||.:....|.|
 Worm  1413 QP----NGGGAVLRQVRPASGL-------------------RERLQDADVRNL------------ 1442

Human  1462 FSPLTAQQMKPEKPSTLQRPQETVIRE---LQPQQQPRTI--ERRDLQYITVSKEELSSGDSLS- 1520
                       ||.:|.....|.|..|   |..:|...||  :||..:.:.|..|:..:...||
 Worm  1443 -----------EKTNTALMSYEAVNEELDRLDAKQNTMTIDEQRRYRELLNVVAEQSRARKLLSE 1496

Human  1521 ----PDPW-KRDAKEKLEKQQQMH----IVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQK 1576
                |..: :|:....::|:||.|    ::|.:......:::....|..||.:::.:....|:|.
 Worm  1497 IRSPPTSYQEREIPIHVQKEQQQHRRETVIDDIPHSSSSVENVSPVSPPESRKVQFIDEVVQYQT 1561

Human  1577 RLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEE 1641
            ....:.....||...    :|:..|:...|..|     |..:||..:|:: |.|.:|:.|
 Worm  1562 PTSGANHAFFDDVPV----LDSPGIVGTNEIYR-----DPRQRRLNELQD-RNRSSENGA----- 1611

Human  1642 RRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLE------PEAP 1689
                 .:..|....:|:|..........|.|:|.::.:|:||:|      |:.|
 Worm  1612 -----ADGAKLGFRDKQRLFARQIGEDTAPRQRMNESSAQRLIETDIAATPQKP 1660

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AFDN	XP_047274744.1	RA1_Afadin	20..132	CDD:340480	75/111 (68%)
		RA2_Afadin	248..348	CDD:340479	32/108 (30%)
		FHA_AFDN	385..503	CDD:438763	38/122 (31%)
		Myo5p-like_CBD_afadin	612..940	CDD:271255	111/349 (32%)
		PDZ_AFDN-like	1013..1100	CDD:467251	49/87 (56%)
		DUF4670	<1470..1688	CDD:464815	53/238 (22%)
afd-1	NP_001021660.2	RA1_Afadin	15..126	CDD:340480	75/113 (66%)
		RA	217..321	CDD:425871	32/109 (29%)
		FHA	354..455	CDD:469597	36/118 (31%)
		Myo5p-like_CBD_afadin	566..867	CDD:271255	111/345 (32%)
		PDZ_AFDN-like	968..1056	CDD:467251	49/87 (56%)
		DUF5585	<1159..>1419	CDD:465521	56/284 (20%)