Protein Alignment AFF1 and HOT1

DIOPT Version :10

• Sequence 1: NP_001160165.1
• Gene: AFF1 / 4299
• HGNCID: 7135
• Length: 1218
• Species: Homo sapiens
• Sequence 2: NP_013895.1
• Gene: HOT1 / 855208
• SGDID: S000004783
• Length: 719
• Species: Saccharomyces cerevisiae
• Alignment Length: 796
• Identity: 155/796 - (19%)
• Similarity: 254/796 - (31%)
• Gaps: 229/796 - (28%)

Human   393 PFPTKDSQHVSSVTQNQK---QYDTSSKTH------SNSQQGT-SSMLEDDLQLSDSEDSDSEQT 447
            |...||...::|......   ..|.|..||      |.|..|: :|.|.:|   .::.|....:|
Yeast    39 PTTLKDGYRMNSQVNEDAIGINLDLSLPTHISPTTGSESASGSNASTLRND---GNALDGGLLRT 100

Human   448 PEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSE--------SESSSSDSEENE- 503
                   ||..|||....:|..:.....:..|..:.:.|:|:|.        |:|.::||..||
Yeast   101 -------SAAISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEI 158

Human   504 --------------------------PLETPAPEPEPPTTNKWQLDNW---------LTKVSQPA 533
                                      .|.....|......|..| ||:         |..|:||:
Yeast   159 STDQLKIFQRMDEMSARMIEMEESFNKLSNKIAEQNTMVLNLKQ-DNYKVMNKLNILLKLVAQPS 222

Human   534 APP-------------------------EGPRSTEPPRRHPESKGSSDSATS---QEHSESKDPP 570
            |.|                         :..::....|:|.....:.||.|.   .:|..:....
Yeast   223 ARPSTNNAQNKLAIELLNSISAVSSAYLQKMQNNGSGRQHTADLCTGDSNTHSGINQHRTTNGTI 287

Human   571 PKSSSKAP----------RAPPEAPHPGKRSCQKSPAQQEPPQRQ---TVGTKQPKKPVKASARA 622
            ..:::.|.          ...|:..|  .|:.......|..|.||   .:.|:..:...:....
Yeast   288 DVNTNTAQLNNQFSNALNTILPDQQH--NRNNVSQNINQSLPNRQLGPVINTQANQNQSQVLIH- 349

Human   623 GSRTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPS 687
            .:.|..|..|.|...|..|.|:..|..|. ..|.|.|...||.       ::....|.|:...|.
Yeast   350 NTNTHQQVNRSPISFPNASTDKPFKLNPN-GIKRRRRNTQSNN-------NASTNDHASAAQKPI 406

Human   688 KALSGPEPAKDNVEDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRD 752
            .|||          ..|..|.:...:..:....|||..|.::.......:........|.||
Yeast   407 SALS----------PLTNSHNSTTSMNYTNSSIHSGVTSASNSFHDLNSLNNFGTTTALSLP--- 458

Human   753 TKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDS-SSK 816
             .|.......||.|:::             ||...:.|      ||.:    .|:..:.|| :|:
Yeast   459 -SLALDNASFPPNQNVI-------------PPIINNTQ------QPLS----FSQLINQDSTTSE 499

Human   817 LAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSS 881
            |....|.....:..|                    :..:.|.||.|...:.|..:..........
Yeast   500 LLPSGKSGVNTNIVN--------------------RNRASTLPSYPKPMTVKSNVDDDGYQEDDD 544

Human   882 QKPAKPALKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRR-VEGKGSRSSSEHKGSSGDTAN 945
            .........|...|..|..:|  :.....:::.|::||.|:|| :..|.|.|.:..:...|::|
Yeast   545 DDGDDEGDGRDNEEDSTAEED--EVDDEIETDMKNASINKRRRSLHHKKSNSLNGRRKLHGESA- 606

Human   946 PFPVPSLPNGNSKPGKPQVKFDKQQADLHMR------EAKKMKQKAELMTDRVGK-AFKYLEAVL 1003
                 :.||.||              |||.|      :.|.:.:  |..|...|| :.|:|||..
Yeast   607 -----TKPNINS--------------DLHYRILKAPTDVKTIWE--EYDTGIRGKPSIKHLEAKY 650

Human  1004 SFIECGIATESESQSSKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMA 1068
            .         ::.:.:|:. ..:|....|.|||:   :..:.....||.|        :::.|..
Yeast   651 G---------NKWRLNKNK-KTFSRRKRLYKFIL---NGMERGKTAQEMI--------ETLENKR 694

Human  1069 MFRCKKDIAIKYSRTL 1084
            :::..:|..:| .||:
Yeast   695 LYKDDEDGEVK-KRTI 709

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AFF1	NP_001160165.1	AF-4	15..527	CDD:461550	38/187 (20%)
		PHA03247	<533..800	CDD:223021	55/307 (18%)
		AF-4_C	957..1218	CDD:465894	28/135 (21%)
HOT1	NP_013895.1	ROM1	242..>437	CDD:227709	41/215 (19%)
		GCR1_C	617..694	CDD:432628	20/99 (20%)