DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Osbp and SWH1

DIOPT Version :9

Sequence 1:	NP_477271.1	Gene:	Osbp / 42985	FlyBaseID:	FBgn0020626	Length:	784	Species:	Drosophila melanogaster
Sequence 2:	NP_009421.3	Gene:	SWH1 / 851286	SGDID:	S000000081	Length:	1188	Species:	Saccharomyces cerevisiae

Alignment Length:	986	Identity:	267/986 - (27%)
Similarity:	414/986 - (41%)	Gaps:	273/986 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 DAAGNALAEKGLPEMKGWLLKWTNYIKGYQRRWFVLSK-GVLSYYRNQSEINHTCRGTISLHGAL 66
            |...|.|.|  .|..||:|.||||:.:||:.|||:||. |.||||.:|::..:.|||::::....
Yeast   272 DVTNNNLHE--APTYKGYLKKWTNFAQGYKLRWFILSSDGKLSYYIDQADTKNACRGSLNMSSCS 334

  Fly    67 IHTVDSC-----TFVISNGGTQTFHIKAGTEVERQSWVTALELAKAKAIRAIECEEEEETETAHV 126
            :| :||.     ..:..|.|...:|:|....:|...||.|::    .|||..: :.|........
Yeast   335 LH-LDSSEKLKFEIIGGNNGVIRWHLKGNHPIETNRWVWAIQ----GAIRYAK-DREILLHNGPY 393

  Fly   127 VPSQEISSVVRDLTDRLESMRTCYDLITKHGAALQRAL--------------------NDLETN- 170
            .||..:|..:.......|::......:||.....:..|                    ||.:.|
Yeast   394 SPSLALSHGLSSKVSNKENLHATSKRLTKSPHLSKSTLTQNDHDNDDDSTNNNNNKSNNDYDDNN 458

  Fly   171 ------------EEES---------LGSRTKIVNE--------RATLFRI--------------- 191
                        ::||         :.||::.::|        ::|:...
Yeast   459 NNNNNDDDDYDDDDESRPLIEPLPLISSRSQSLSEITPGPHSRKSTVSSTRAADIPSDDEGYSED 523

  Fly   192 -------TSNAMINAG---------------------------------NDYLHTAEAQGHKWSK 216
                   :|..|.|.|                                 |:.|...:.....|:.
Yeast   524 DSDDDGNSSYTMENGGENDGDEDLNAIYGPYIQKLHMLQRSISIELASLNELLQDKQQHDEYWNT 588

  Fly   217 M----------------LHHEREQRQRLEEIIEQMAKQQSQMEQAAVLVRQNKPVSSTSGAGTAG 265
            :                |..:||:|     :|.||.||:..         .|..:.|
Yeast   589 VNTSIETVSEFFDKLNRLTSQREKR-----MIAQMTKQRDV---------NNVWIQS-------- 631

  Fly   266 SLVTSDEEMEFFDAEEHGYSGSGRSPESSDCERGTFILKMHKRRSSSEDQVEGHLEGSSSESDEQ 330
               ..|.|||..|.:|        ...:.|.||.. :.||.:::.:::.|||..   ::.|||:.
Yeast   632 ---VKDLEMELVDKDE--------KLVALDKERKN-LKKMLQKKLNNQPQVETE---ANEESDDA 681

  Fly   331 KRTVQTVQQ--------VCLVSAPRVASGGQGDDDDDVDKALPAKE-------------STDSIY 374
            ...::..|:        |..:.|.:     :.|:|.|.|:...|:|             :|:...
Yeast   682 NSMIKGSQESTNTLEEIVKFIEATK-----ESDEDSDADEFFDAEEAASDKKANDSEDLTTNKET 741

  Fly   375 GRNWNP----------------------DLIKK----------RRDRVPDKPNHP-ISLWGIMKN 406
            ..|..|                      .|:|:          |.....|:.|.| |.||.::|:
Yeast   742 PANAKPQEEAPEDESLIVISSPQVEKKNQLLKEGSFVGYEDPVRTKLALDEDNRPKIGLWSVLKS 806

  Fly   407 CIGKDLSKIPMPINFNEPLSMLQRLVEDYEYTEILDYAATCQDECEQLAYIAAFTVSAYATTTNR 471
            .:|:||:|:.:|::||||.|:|||:.||.||:.|||.|||.:|...::.|:||||.|.||:||||
Yeast   807 MVGQDLTKLTLPVSFNEPTSLLQRVSEDIEYSHILDQAATFEDSSLRMLYVAAFTASMYASTTNR 871

  Fly   472 TGKPFNPLLGETYECDRMDDYGWRCLAEQVSHHPPVAALHCESKNWTCWQEFSMTSKFRGKYVQI 536
            ..||||||||||:|..|.|.. :|...||||||||::|...||..|..:.|.::.|.|.|:...:
Yeast   872 VSKPFNPLLGETFEYARTDGQ-YRFFTEQVSHHPPISATWTESPKWDFYGECNVDSSFNGRTFAV 935

  Fly   537 NPLGGVYVQF-PNSG-----RRYSWRKVTTTVNNIIVGKLWVDQHGEMEIRGSQAAEGHKCILNF 595
            ..||..|:.. |:..     ..|||:|...||..|::||..||..|::::  :....|..|:|::
Yeast   936 QHLGLWYITIRPDHNISVPEETYSWKKPNNTVIGILMGKPQVDNSGDVKV--TNHTTGDYCMLHY 998

  Fly   596 IPYSYFSRDVQRSVKGVVMNKDNEVKWVVRGTWDMKIEIAPV-----------LKTSGSVSSPTY 649
            ..:.:.|.... .|:|.|.|||::..||:.|.|:..|....|           :||:.|.:....
Yeast   999 KAHGWTSAGAY-EVRGEVFNKDDKKLWVLGGHWNDSIYGKKVTARGGELTLDRIKTANSATGGPK 1062

  Fly   650 TTGEFKLAWR--RRPAPPDSDKFYNFTTLACQLNEEEEG----VAPTDSRLRPDQRLMEQGDWDE 708
            ..|...|.|:  .||:.|     :|.|:.|..||.....    :||||||||||||.||.|::|:
Yeast  1063 LDGSKFLIWKANERPSVP-----FNLTSFALTLNALPPHLIPYLAPTDSRLRPDQRAMENGEYDK 1122

  Fly   709 SNKEKLRLEEKQRTERRRRENEAEEAAAEGRPYPAYEPMWFKREKEEGSEEYVHVFKNTYWEAKA 773
            :..||.|:|.|||..::.||.:.||          |.|.||.:|:...::.....|...||..:.
Yeast  1123 AAAEKHRVEVKQRAAKKEREQKGEE----------YRPKWFVQEEHPVTKSLYWKFNGEYWNKRK 1177

  Fly   774 AQNFEGCPDIY 784
            ..:|:.|.||:
Yeast  1178 NHDFKDCADIF 1188

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Osbp	NP_477271.1	PH	15..107	CDD:278594	35/97 (36%)
		PH_OSBP_ORP4	17..113	CDD:270101	37/101 (37%)
		Oxysterol_BP	399..764	CDD:279564	147/387 (38%)
SWH1	NP_009421.3	Ank_4	56..117	CDD:372654
		ANK repeat	56..94	CDD:293786
		ANKYR	<89..181	CDD:223738
		ANK repeat	96..193	CDD:293786
		ANK repeat	195..227	CDD:293786
		Ank	197..228	CDD:394980
		PH_Osh1p_Osh2p_yeast	281..383	CDD:241446	38/107 (36%)
		SMC_prok_A	<562..>774	CDD:274009	44/253 (17%)
		Oxysterol_BP	800..1174	CDD:395990	149/392 (38%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	255	1.000	Domainoid score	I325
eggNOG	1	0.900	-	-		E1_KOG1737
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	330	1.000	Inparanoid score	I427
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46720
orthoMCL	1	0.900	-	-		OOG6_101373
Panther	1	1.100	-	-	O	PTHR10972
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3013
SonicParanoid	1	1.000	-	-		X203
TreeFam	1	0.960	-	-
	11	10.850