DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Osbp and Osbpl1a

DIOPT Version :9

Sequence 1:	NP_477271.1	Gene:	Osbp / 42985	FlyBaseID:	FBgn0020626	Length:	784	Species:	Drosophila melanogaster
Sequence 2:	NP_997413.2	Gene:	Osbpl1a / 64291	MGIID:	1927551	Length:	950	Species:	Mus musculus

Alignment Length:	834	Identity:	259/834 - (31%)
Similarity:	391/834 - (46%)	Gaps:	165/834 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 GNALAEKGLPEMKGWLLKWTNYIKGYQRRWFVLSKGVLSYYRNQSEINHTC--RGTISLHGAL-- 66
            ||.:..|.|...:|.|.|.:.:. |::..|.||..||||:||.|.:..|..  :|...|..|:
Mouse   227 GNKVVHKALKRYEGPLWKSSRFF-GWKLFWVVLEHGVLSWYRKQPDAVHNSYRQGCKHLTQAVCT 290

  Fly    67 IHTVDSCTFVIS--NGGTQTFHI-KAGTEVERQSWVTALELAKAKAIRAIECEEEEETETAHVVP 128
            :...|||.|.|.  :.....|.: |...:..|:.|:.|:|  :..|.....|.:::.|:.    .
Mouse   291 VKPTDSCLFSIRCFDDTVHCFRVPKNSVQQSREKWLEAIE--EHSAYSTHYCSQDQVTDD----E 349

  Fly   129 SQEISSVVRDLTDRLESMRTCYDLITKHGAALQRALNDLETNEE--ESLGSRTKIVNERATLFRI 191
            .:::.|.: ||.:.|...:||...:.:......:.:.:.:..::  .|...:..||:|.:   |.
Mouse   350 EEDVVSAM-DLKESLARAQTCQQRLDREIYNFLKMIKECDVAKDMLPSFLQKADIVSEAS---RE 410

  Fly   192 TSNAMINAGNDYLHTAEAQGHKWSKMLHHEREQRQRLEEIIEQMAKQQSQMEQAAVLVRQNKPVS 256
            |..|:    ||.|:....|....:..|..|:|:.:.|.|.:|.:|.:..::|::  ||..:.|||
Mouse   411 TCVAL----NDCLNLFTKQEGVRNFKLEQEQEKNKILSEALETLATEHHELERS--LVEGSPPVS 469

  Fly   257 STSGAGTAGSLVTSDEEMEFFDAEEHGYSGSGRSPESSDCERGTFILKMHKRRSSSEDQVEGHLE 321
            ..|             |.||:||    .||| .|..|..|      |:.....|..|::|    .
Mouse   470 ILS-------------EEEFYDA----LSGS-ESEGSLTC------LEAVTAHSFEENEV----P 506

  Fly   322 GSSSESDEQKRTVQTVQQVCLVSAPRVASG---GQGDDDDDVDKALPAKESTDSIYGRNWNPDLI 383
            |||.:.                   |::.|   |.||       ||               .:.|
Mouse   507 GSSGKH-------------------RMSEGKDCGGGD-------AL---------------SNGI 530

  Fly   384 KKRRDRVPDK--PNHPISLWGIMKNCIGKDLSKIPMPINFNEPLSMLQRLVEDYEYTEILDYAAT 446
            ||.|..:|..  ..:..|:|.|::.|||.:||||.||:.||||||.||||.|..|:|.::..|::
Mouse   531 KKHRTSLPSPMFSRNDFSIWSILRKCIGMELSKITMPVIFNEPLSFLQRLTEYMEHTYLIHKASS 595

  Fly   447 CQDECEQLAYIAAFTVSAYATTTNRTGKPFNPLLGETYECDRMDDYGWRCLAEQVSHHPPVAALH 511
            ..|..|::..:|||.|||.|:...|||||||||||||||..| ||.|:|.::||||||||::|.|
Mouse   596 LSDPVERMQCVAAFAVSAVASQWERTGKPFNPLLGETYELVR-DDLGFRLISEQVSHHPPISAFH 659

  Fly   512 CESKNWTCWQEFSMTS------KFRGKYVQINPLGGVYVQFPNSGRRYSWRKVTTTVNNIIVGKL 570
            .|..|    .:|....      ||.||.|:..|.|.:.::..:....|:|...|..|:|||||||
Mouse   660 AEGLN----NDFIFHGSIYPKLKFWGKSVEAEPKGTITLELLDHNEAYTWTNPTCCVHNIIVGKL 720

  Fly   571 WVDQHGEMEIRGSQAAEGHKCILNFIPYSYFSRDVQRSVKGVVMNKDNEVKWVVRGTW-DMKIEI 634
            |::|:|.:||...:.  |.||:|||.|...|.:::.: |:|.:.:|..:....:.|.| :....:
Mouse   721 WIEQYGNVEIINHKT--GDKCVLNFKPCGLFGKELHK-VEGYIQDKSKKKLCALYGKWTECLYSV 782

  Fly   635 APVL-------------------KTSGSVSSPTYTTGEFK---------LAWRRRPAPPDSDKFY 671
            .|..                   :||.|..|......:.:         |.||..|.||:|.:.|
Mouse   783 DPATFDAYKKNDKKNTEEKKNSKQTSSSEESDEMPVPDSESVFIIPGSVLLWRIAPRPPNSAQMY 847

  Fly   672 NFTTLACQLNE---EEEGVAP-TDSRLRPDQRLMEQGDWDESNKEKLRLEEKQRTERRRRENEAE 732
            |||:.|..|||   |.|.|.| ||.|||||.|.||.|:.|.:::||.|||||||..|:.|....|
Mouse   848 NFTSFAMVLNEVDKEMESVIPKTDCRLRPDIRAMENGEIDLASEEKKRLEEKQRAARKNRSKSEE 912

  Fly   733 EAAAEGRPYPAYEPMWFKR--EKEEGSEEYVHVFKNTYWEAKAAQNFEGCPDIY 784
            :          ::..||.:  ....|::::  ::..:||:    :|:...||||
Mouse   913 D----------WKTRWFHQGPNPYSGAQDW--IYSGSYWD----RNYFNLPDIY 950

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Osbp	NP_477271.1	PH	15..107	CDD:278594	29/98 (30%)
		PH_OSBP_ORP4	17..113	CDD:270101	30/102 (29%)
		Oxysterol_BP	399..764	CDD:279564	155/405 (38%)
Osbpl1a	NP_997413.2	Interaction with RAB7A. /evidence=ECO:0000250	1..237		4/9 (44%)
		Ank_2	10..111	CDD:372319
		ANKYR	<38..208	CDD:223738
		ANK repeat	43..78	CDD:293786
		ANK repeat	80..111	CDD:293786
		ANK repeat	175..206	CDD:293786
		PH_ORP1	229..352	CDD:270102	34/129 (26%)
		FFAT. /evidence=ECO:0000250\|UniProtKB:Q9BXW6	469..483		8/30 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	502..530		12/72 (17%)
		Oxysterol_BP	548..940	CDD:366530	156/411 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	795..821		4/25 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1737
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3013
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.840