Protein Alignment Osbp and osh2

DIOPT Version :10

• Sequence 1: NP_477271.1
• Gene: Osbp / 42985
• FlyBaseID: FBgn0020626
• Length: 784
• Species: Drosophila melanogaster
• Sequence 2: NP_595710.1
• Gene: osh2 / 2540357
• PomBaseID: SPBC2F12.05C
• Length: 1310
• Species: Schizosaccharomyces pombe
• Alignment Length: 1124
• Identity: 299/1124 - (26%)
• Similarity: 428/1124 - (38%)
• Gaps: 399/1124 - (35%)

  Fly     5 AGN--ALAEKGLPE-MKGWLLKWTNYIKGYQRRWFVLSKGVLSYYRNQSEINHTCRGTISLHGA- 65
            :||  :.:...:|: |.|:|.|||||..||:.|||.|:.||||||:||.:.:..|||:|:|..|
pombe   242 SGNRDSSSAPAVPQHMSGYLKKWTNYKSGYKLRWFTLNNGVLSYYKNQDDASSACRGSINLKLAR 306

  Fly    66 LIHTVDSCT-FVISNGGTQTFHIKAGTEVERQSWVTALELAKAKAIRAIECEEEEETETAHVVPS 129
            |:|.....| |.:...|:..:.:||.:.||.:.|:.|:.       .|||.:||.....   |.:
pombe   307 LVHDPKQPTVFQVIGKGSVRYSVKANSPVEAKKWIAAIS-------SAIENDEEANKPN---VTA 361

  Fly   130 QEISSVVRDLTDRLESMRTCYDLITKHGAALQRALNDLETN-EEESLGSRTKI---VN----ERA 186
            ...|....||......       .|:..|:.....:|.|:: :...|.||...   ||    :..
pombe   362 DNASFGTHDLAPAAHK-------FTQSNASGNSGWDDNESDIDRAQLPSRENFEFNVNIAKLQVE 419

  Fly   187 TLFRITSNAM-----------------INAGNDYLH----------------------TAEAQGH 212
            ||.::..:||                 |:...:.||                      .|:|...
pombe   420 TLHKLIDSAMQTEKVKKDPSLSQVFDGISNSFNTLHKTVLEMLDLQRQAEHYYKRKLDNAKAINA 484

  Fly   213 KWSKML--------------HHEREQRQRLEEIIEQMA----KQQSQMEQAAVLVRQNKPVSSTS 259
            .|::.|              |.....|:|.:....::|    |:.|..:....:...:..:||||
pombe   485 LWAENLKTVVEEQDQIEERYHRSEAHRRRTKRAFRRLAASLKKRPSDKDNKLHIHYDDGAMSSTS 549

  Fly   260 GAGTAGSLVTSDEEM-----EFFDAEEHGYSGSGR------------------------------ 289
                ..:..|.|||.     ||||.:.|..:.:.:
pombe   550 ----YSTDFTDDEEETNTKDEFFDVDAHDNNHANKAEPSQTANNVHEIREPSFSSEHKPQPSLKT 610

  Fly   290 -----SPES----SDCERGTF--------------------------------------ILKMHK 307
                 |||:    :|..:.|.                                      .:..|.
pombe   611 TTDVSSPETKQNIADIRKTTLTDQTEEFTERRDNNGSIPSKQPSDEQHLGKESLPSQQSTVSNHH 675

  Fly   308 RRSS----------------------------------SEDQVEG-----------------HL- 320
            |:.|                                  |:...||                 ||
pombe   676 RKESIPSKQPTEGQHARQESLPSQQTTETKHLRKESTPSKQPTEGQHTRQESLPSQQTTETKHLR 740

  Fly   321 ----------EGS----------------------------------------SSESDEQKRTVQ 335
                      ||.                                        |.:|.|.|::.|
pombe   741 KESIPSKQPTEGQHARQESLPSQQTTETKHLRKESIPSKQPSGGQQLRQESLPSQQSSESKQSTQ 805

  Fly   336 TVQQVCL-------VSAPRV----------------ASGGQGDDDDDVDKALP------------ 365
            ..|.|.:       ||||:.                ||....:.||...|..|
pombe   806 HQQPVEVQKSIQSDVSAPKAKEVSEKPVSHQAKPSNASQLSRNTDDTQAKEAPKEASIPDNASTA 870

  Fly   366 -AKESTDSIYGRNWNPDLIKK-----------------------------------RRDRVPDKP 394
             .|.|.||    :..||..||                                   .|:.:....
pombe   871 STKVSNDS----HLKPDADKKSVSSELTHASKPSLDEKTMQLAKQIAVSFHGYEAPTRENLDVND 931

  Fly   395 NHP-ISLWGIMKNCIGKDLSKIPMPINFNEPLSMLQRLVEDYEYTEILDYAATCQDECEQLAYIA 458
            :.| ||||||:|..||||::|:.:|::||||.|:|||:.||.||||:||.|:..:|..:::.|:|
pombe   932 DRPKISLWGILKGMIGKDMTKMTLPVSFNEPTSLLQRVAEDMEYTELLDQASHNKDPYQRILYVA 996

  Fly   459 AFTVSAYATTTNRTGKPFNPLLGETYE-CDRMDDYGWRCLAEQVSHHPPVAALHCESKNWTCWQE 522
            ||..|.||:|.||..|||||||||||| |.  ...|:|.:.|||||||||.|.:.||.||..:.|
pombe   997 AFAASEYASTLNRVAKPFNPLLGETYEFCH--PQRGFRFIVEQVSHHPPVGAAYSESANWKYYGE 1059

  Fly   523 FSMTSKFRGKYVQINPLGGVYVQFPN-SG--RRYSWRKVTTTVNNIIVGKLWVDQHGEMEIRGSQ 584
            .|:.|||.||...|:|||..:::..: ||  ..|:|:|||::|..||:|...||.:|:|.|  ..
pombe  1060 SSVKSKFYGKSFDISPLGTWFLELRHPSGEVELYTWKKVTSSVVGIILGSPSVDNYGQMHI--VN 1122

  Fly   585 AAEGHKCILNFIPYSYFSRDVQRSVKGVVMNKDNEVKWVVRGTWDMKIEIAPVLKTSGSVSSPTY 649
            .:.|..|:|:|.|..:...:. ..|||.|.:.|:..||:|.|.|:.||             ....
pombe  1123 HSSGINCVLDFKPRGWRGTNA-HEVKGSVQSTDDTPKWMVNGHWNDKI-------------FGQQ 1173

  Fly   650 TTGEFKLAWRRRPAPPDSDKFYNFTTLACQLN----EEEEGVAPTDSRLRPDQRLMEQGDWDESN 710
            ..|...|.|:....||   :.:|.|..|..||    :.:..:.|||:|||||||.||.|.:|.:.
pombe  1174 PNGNKILLWQNHERPP---RPFNLTPFAISLNALTPQLKPWLPPTDTRLRPDQRAMENGQYDLAA 1235

  Fly   711 KEKLRLEEKQRTERRRRENEAEEAAAEGRPYPAYEPMWFKREKEEGSEEYVHVFKNTYWEAK--- 772
            .||.|||||||.:||.||        :|. .|.:.|.||...|...:.|....|.|.||:.:
pombe  1236 SEKNRLEEKQRKKRRMRE--------QGE-MPPWSPRWFSAAKHPVTGEDYWQFNNEYWKIREEA 1291

  Fly   773 -----AAQNFE--GCPDIY 784
                 |.:.||  ...||:
pombe  1292 GEAHLAGKEFEWPNVDDIF 1310

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Osbp	NP_477271.1	PH_OSBP_ORP4	17..113	CDD:270101	39/97 (40%)
		Oxysterol_BP	399..773	CDD:460126	161/389 (41%)
osh2	NP_595710.1	ANKYR	18..239	CDD:440430
		ANK repeat	58..86	CDD:293786
		ANK repeat	88..185	CDD:293786
		ANK repeat	188..219	CDD:293786
		PH_Osh1p_Osh2p_yeast	254..354	CDD:241446	44/106 (42%)
		Semenogelin	<563..891	CDD:368458	45/331 (14%)
		Oxysterol_BP	937..1289	CDD:460126	161/381 (42%)