Protein Alignment niki and Nek2

DIOPT Version :10

• Sequence 1: NP_651293.1
• Gene: niki / 42959
• FlyBaseID: FBgn0045980
• Length: 841
• Species: Drosophila melanogaster
• Sequence 2: NP_035022.2
• Gene: Nek2 / 18005
• MGIID: 109359
• Length: 443
• Species: Mus musculus
• Alignment Length: 520
• Identity: 147/520 - (28%)
• Similarity: 233/520 - (44%)
• Gaps: 131/520 - (25%)

  Fly    77 MPNRQESLLQLSVPRETGVGVAGPELANYEKVRVVGQGSFGIAILYRRKSDGHQIVFKQINLSEL 141
            ||:|.|                     :||.:..:|.||:|.....||||||..:|:|:::...:
Mouse     1 MPSRVE---------------------DYEVLHSIGTGSYGRCQKIRRKSDGKILVWKELDYGSM 44

  Fly   142 SPPGRDLAMNEVDVFSKLHHPNIVSYLGSFI--KDNTLLIEMEYADGGTLAQIIAE-RQGKLHFP 203
            :...:.:.::||::..:|.|||||||....|  .:.||.|.|||.:||.||.:|:: .:.:.:..
Mouse    45 TEVEKQMLVSEVNLLRELKHPNIVSYYDRIIDRTNTTLYIVMEYCEGGDLASVISKGTKDRQYLE 109

  Fly   204 ERYIIAVFEQISSAINYMH-----SENILHRDLKTANVFLNRRGIVKIGDFGISKIMNTKIH--- 260
            |.:::.|..|::.|:...|     ...:||||||.|||||:.:..||:||||:::|:|   |
Mouse   110 EEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDSKHNVKLGDFGLARILN---HDTS 171

  Fly   261 -AQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILGEMCCLKKTFAASNLSELVTKIMAGNYTPV 324
             |:|.:|||||.|||......|:.|||||:|||:|.|:|.|...|.|.|..||..||..|.:..:
Mouse   172 FAKTFVGTPYYMSPEQMSCLSYNEKSDIWSLGCLLYELCALMPPFTAFNQKELAGKIREGRFRRI 236

  Fly   325 PSGYTSGLRSLMSNLLQVEAPRRPTASEVLVYWIPLIFRSLGKNKGYSYEDDVGGPGSDQLTAPV 389
            |..|:.||..|::.:|.::...||:..|:|.  .|||...:.:.:..:.|......|.     |.
Mouse   237 PYRYSDGLNDLITRMLNLKDYHRPSVEEILE--SPLIADLVAEEQRRNLERRGRRSGE-----PS 294

  Fly   390 PAAAYSNVSMELELPTAQTETKQLMIADTAAPHEILEKR-------------------------S 429
            .....|.|..||:|...|.:.::..:   .|..:|||::                         |
Mouse   295 KLPDSSPVLSELKLKERQLQDREQAL---RAREDILEQKERELCIRERLAEDKLARAESLMKNYS 356

  Fly   430 VLYQLKAFGTCFSMAP-IQLPPKAVIVDVAMSDSHFVVVNEDGSAYAWGEGTHGQLGLTALEAWK 493
            :|.:.:..  |.:..| :.||..|:...|     ||                ||:.        |
Mouse   357 LLKEHRLL--CLAGGPELDLPSSAMKKKV-----HF----------------HGES--------K 390

  Fly   494 HYPSRMESVRNYHVVSACAGDGFTILVTQAGSLLSCGSNAHLALGQDEQRNYHSPKLIAR-LADV 557
            ...:|.|:..:|...|.|                           :|.::..|:.:|.|: |||:
Mouse   391 ENTARSENSESYLAKSKC---------------------------RDLKKRLHAAQLRAQALADI 428

  Fly   558  557
            Mouse   429  428

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
niki	NP_651293.1	STKc_Nek	104..359	CDD:270855	102/266 (38%)
		ATS1	433..749	CDD:444065	23/127 (18%)
Nek2	NP_035022.2	STKc_Nek2	7..271	CDD:270857	103/268 (38%)
		Interaction with PCNT. /evidence=ECO:0000250	264..443		43/233 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	282..303		4/25 (16%)
		Interaction with CEP85. /evidence=ECO:0000250|UniProtKB:P51955	301..443		34/189 (18%)
		Leucine-zipper	306..334		7/30 (23%)
		Necessary for interaction with MAD1L1. /evidence=ECO:0000250	329..443		25/158 (16%)
		Required for microtubule binding and for localization to the centrosomes. /evidence=ECO:0000250	333..370		3/38 (8%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	383..402		8/47 (17%)
		Interaction with SAV1 and STK3/MST2. /evidence=ECO:0000250	402..437		10/54 (19%)