DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment niki and nek8

DIOPT Version :9

Sequence 1:	NP_651293.1	Gene:	niki / 42959	FlyBaseID:	FBgn0045980	Length:	841	Species:	Drosophila melanogaster
Sequence 2:	NP_620776.1	Gene:	nek8 / 171094	ZFINID:	ZDB-GENE-020509-1	Length:	697	Species:	Danio rerio

Alignment Length:	690	Identity:	224/690 - (32%)
Similarity:	338/690 - (48%)	Gaps:	100/690 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly   102 LANYEKVRVVGQGSFGIAILYRRKSDGHQIVFKQINLSELSPPGRDLAMNEVDVFSKLHHPNIVS 166
            :..|||.:|||:|:|||..|.||::|...::.|:|.:.:::...|..|.||..|...|.||||:.
Zfish     1 MEKYEKTKVVGRGAFGIVHLCRRRTDSALVILKEIPVEQMTRDERLAAQNECQVLKLLSHPNIIE 65

  Fly   167 YLGSFIKDNTLLIEMEYADGGTLAQIIAERQGKLHFPERYIIAVFEQISSAINYMHSENILHRDL 231
            |..:|::|..|:|.||||.|||||..|.:|...| ..|..|:..|.||..|:.::|::.||||||
Zfish    66 YYENFLEDKALMIAMEYAPGGTLADYIQKRCNSL-LDEDTILHSFVQILLALYHVHNKLILHRDL 129

  Fly   232 KTANVFLNR-RGIVKIGDFGISKIMNTKIHAQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILG 295
            ||.|:.|:: :.||||||||||||:.:|..|.||:|||.|.|||:||||.|:.|||||||||:|.
Zfish   130 KTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLY 194

  Fly   296 EMCCLKKTFAASNLSELVTKIMAGNYTPVPSGYTSGLRSLMSNLLQVEAPRRPTASEVLVYWI-- 358
            |:..||:.|.|:||..||.|||:|.:.|:...|:..||.|:.|:|.::..:||..:|::.:.|
Zfish   195 ELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMAHAICI 259

  Fly   359 -PL--IFRSLGKNKGYSYEDDV--------GGPG----------------SDQLTAPVPA-AAY- 394
             ||  ::..:|..|....|..:        |.||                |.::..|:|. :.|
Zfish   260 RPLLNLYTDIGNVKMRRIEKPLSNVQAGPHGRPGGWITSTRTRGGLSSLTSSKMMHPLPLFSVYT 324

  Fly   395 --SNVSMELELPTAQTETKQLMIADTAAPHEILEKRSVLYQLKAFGTCFSMAP---IQLPPKAVI 454
              |.:|..|.||...||..|:.:..|.........|.:.::..:.|:.....|   .|:.|:.:
Zfish   325 WGSGISTPLRLPMLNTEVIQVSLGRTQKMGVTKSGRLITWEAPSVGSGEPTLPGAVEQMQPQFI- 388

  Fly   455 VDVAMSDSHFVVVNEDGSAYAWGEGTHGQLGLTALEAWKHYPSRMESVRNYHVVSACAGDGFTIL 519
                   |.|:               .||.|:|                   :.|...||.||..
Zfish   389 -------SRFL---------------EGQSGVT-------------------IKSVSCGDLFTTC 412

  Fly   520 VTQAGSLLSCGSNAHLALGQDEQRNYHSPKLIARLADVRVEQVAAGLQHVLALSREGAVYVWGTS 584
            :|..|.:::.||.::..||.....:...||::..|....:.||:.|..||||::.|..|:.||..
Zfish   413 LTDRGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYELVQVSCGASHVLAVTNEREVFSWGRG 477

  Fly   585 TCGALGLGNYQQQQKFPQKILLSHVKTKPSKIYCGPDTSAVLFANGELHVCGSNDYNKLGFQR-- 647
            ..|.|||.. |.....||::.|. ...:..::.||.|.|.::....::..||:|.:||||..:
Zfish   478 DNGRLGLAT-QDSHNCPQQVSLP-ADFEAQRVLCGVDCSMIMSTQHQILACGNNRFNKLGLDKVS 540

  Fly   648 -SAKITAFKKVQLPH-------------KVTQACFSSTHSVFLVEGGYVYTMGRNAEGQRGIRHC 698
             :.:.::|.:|:..|             |:......:.|||.:.|.|..:|.|.|..||.|..|.
Zfish   541 GTEEPSSFCQVEEVHLFQLVQSAPLNTEKIVYIDIGTAHSVAVTEKGQCFTFGSNQHGQLGCSHR 605

  Fly   699 NSVDHPTLVDSVKSRYIVKANCSDQCTIVASEDNIITVWG 738
            .|...|..|..::.  |..|.|.|..|:....:..:..||
Zfish   606 RSSRVPYQVSGLQG--ITMAACGDAFTLAIGAEGEVYTWG 643

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
niki	NP_651293.1	STKc_Nek	104..359	CDD:270855	122/258 (47%)
		S_TKc	105..354	CDD:214567	121/249 (49%)
		ATS1	445..747	CDD:227511	79/313 (25%)
nek8	NP_620776.1	STKc_Nek8	3..258	CDD:270859	121/255 (47%)
		S_TKc	4..255	CDD:214567	121/251 (48%)
		ATS1	309..662	CDD:227511	94/381 (25%)
		RCC1 1	417..468		15/50 (30%)
		RCC1 2	469..520		15/52 (29%)
		RCC1 3	521..586		14/64 (22%)
		RCC1 4	587..636		16/50 (32%)
		RCC1 5	637..689		2/7 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0589
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H84442
Inparanoid	1	1.050	329	1.000	Inparanoid score	I2440
OMA	1	1.010	-	-		QHG48158
OrthoDB	1	1.010	-	-		D1062377at2759
OrthoFinder	1	1.000	-	-		FOG0000168
OrthoInspector	1	1.000	-	-		oto40999
orthoMCL	1	0.900	-	-		OOG6_106447
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R445
SonicParanoid	1	1.000	-	-		X891
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	12	11.770