Protein Alignment puf and USP9X

DIOPT Version :9

• Sequence 1: NP_001262923.1
• Gene: puf / 42935
• FlyBaseID: FBgn0039214
• Length: 3930
• Species: Drosophila melanogaster
• Sequence 2: XP_005272732.1
• Gene: USP9X / 8239
• HGNCID: 12632
• Length: 2575
• Species: Homo sapiens
• Alignment Length: 3142
• Identity: 577/3142 - (18%)
• Similarity: 1003/3142 - (31%)
• Gaps: 1169/3142 - (37%)

  Fly   102 QQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTAN 166
            |.|...|:::.|:...:.|:| ||..|           :.||.||.|   |.:....|..|.|
Human    23 QSQPPLQQNQTSSPDSSNENS-PATPP-----------DEQGQGDAP---PQLEDEEPAFPHT-- 70

  Fly   167 PQHKSHLQYTEEPWILPEVEK--LLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQ----------- 218
            ...|.........|::|.:.|  |.||:.....|:       |.|:..:.:..|
Human    71 DLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLS-------KKGLDVKSEACQRFFRDGLTISF 128

  Fly   219 ----AEEA----------------HHLALIC--DLHDNDLPIYLL-------------------- 241
                .:||                |.|..:|  .|..:..|:..|
Human   129 TKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPC 193

  Fly   242 RNVS---------LFCNS--------------GGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKL- 282
            .:||         ||..|              ..||.::      ...:.....:..:..||::
Human   194 ESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLN------GFQILHDRFINGSALNVQII 252

  Fly   283 ------------WLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVA 335
                        :|..|...:.|:|:...:.|::..|:|:.|:..|..       .:..|.|.:.
Human   253 AALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKN-------EAKNDALSMI 310

  Fly   336 VNFDTEGLALAFKYFTSTT---------LTMRLAGMAQINAHINLFNEICTTETVNEVE------ 385
            :. ..:.||........|.         :.:||..::..|..:|..||:  .:.::.|.
Human   311 IK-SLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEV--NKVISSVSYYTHRH 372

  Fly   386 --------LFGQRIANWLTENHIVQHLFGPNLH-VEIVKQAHVLLNFLAVENQISEEDIKLIWQA 441
                    |..:|:|.|:.:|:|:..:...:|| .:.|::...:|.|:..|..::.:|:..||.|
Human   373 GNPEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAA 437

  Fly   442 TQLKH--CSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSR 504
            ...||  ..|.:.|:|..|..:.:|....||:...                    |..||..:.:
Human   438 QAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCF--------------------KASWTNASKK 482

  Fly   505 SQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQ 569
            .:..|::                 :.....|||.                       |||
Human   483 QREKLLE-----------------LIRRLAEDDK-----------------------DGV----- 502

  Fly   570 ARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSN 634
                                  ||      .:::|::.|.:..::
Human   503 ----------------------MA------HKVLNLLWNLAHSDD-------------------- 519

  Fly   635 KRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAV 699
                        :|.:::::                        .|:...:..|.:.||   |..
Human   520 ------------VPVDIMDL------------------------ALSAHIKILDYSCSQ---DRD 545

  Fly   700 LKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSR 764
            .:.:|....||..:.||                              |:.|.|..:::.::
Human   546 TQKIQWIDRFIEELRTN------------------------------DKWVIPALKQIREI---- 576

  Fly   765 ANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVA 829
              ||                                          ..|.|.|.|
Human   577 --CS------------------------------------------LFGEAPQNL---------- 587

  Fly   830 AAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWD 894
                                            .:|:.|.|..|..|
Human   588 --------------------------------SSSRFSQTQRSPHV------------------- 601

  Fly   895 LLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFAS 959
            ..:.|.|.|      |:...||.||                    ||.|
Human   602 FYRHDLINQ------LQHNHALVTL--------------------VAEN---------------- 624

  Fly   960 FQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVS 1024
                                  :..:..::|.|||.|      ::.:.|..:||.: |||..:|.
Human   625 ----------------------LATYMESMRLYARDH------EDYDPQTVRLGSR-YSHVQEVQ 660

  Fly  1025 VRLQFLSSIFSTVGSPKSFRLTL--EQLDALWEWLAH------DPECADCYFSWLQAQAKGGDQH 1081
            .||.||..:.      |..:|.|  .|...:|:.||.      |.|....::|.|.     ||:.
Human   661 ERLNFLRFLL------KDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLM-----GDEP 714

  Fly  1082 ALGIEALQHLYLKKLPELRPEEFSMVALGLFQQL-----CSFARIAMAEYDNHSDQISASASAVG 1141
            .|..:..:..:...:.:|.|...:...:..|::.     |...::.........|.:    ..:|
Human   715 DLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDL----ELIG 775

  Fly  1142 MYHLWKIALRAQSN-DVSLAAIQYINMYY--MGQQLR-----LEKEFVSQCMENLVQAATALESI 1198
            :.:||::.:  ||| |::..||..:...|  :|.:|:     :.::|:..|.:.|..:...|..:
Human   776 LDYLWRVVI--QSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVL 838

  Fly  1199 D-DENAL-------MRVQRGLLLLNTHLD-----------------TFRRRYAFHLRRWAIEGKG 1238
            | |::::       :|:.|.|.:|..:::                 .||.::...:.|:..:|:.
Human   839 DGDKDSVNCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFVVRFPNQGRQ 903

  Fly  1239 IG-----SHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAP 1298
            :.     ||:   |:..|...|.:|.:                   :||.|           |..
Human   904 VDDLEVWSHT---NDTIGSVRRCILNR-------------------IKANV-----------AHT 935

  Fly  1299 VLGLLLSDGPLRIITQGQELTSDYDERSL-GDAGFKDNQIVYVSL----------------GGRG 1346
            .:.|.:          |.||....|:|.| |....||..::...|                ...|
Human   936 KIELFV----------GGELIDPADDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTG 990

  Fly  1347 ARRKESNLEHPSMLPPPPKE-CLPTVLL-LQPKYFEKLFCLMQTLGDMQPQASTVN-PQHHTKAQ 1408
            :.....|  |.|..|.|..| |||.|:: |.|:|...|:    .:.|:   .|::| |.....|:
Human   991 SPGNHGN--HYSDGPNPEVESCLPGVIMSLHPRYISFLW----QVADL---GSSLNMPPLRDGAR 1046

  Fly  1409 LLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNL 1473
            :|       :.::|.:...::..:::..|.::|      ||        ..:......|....:.
Human  1047 VL-------MKLMPPDSTTIEKLRAICLDHAKL------GE--------SSLSPSLDSLFFGPSA 1090

  Fly  1474 QKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKS-------SMGRRRNSNEQPPPPPP 1531
            .:.:|...:|.:|.:      .:...:|..::..|....||       ||..|.|.       .|
Human  1091 SQVLYLTEVVYALLM------PAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNF-------LP 1142

  Fly  1532 EVKMSKEQLCELE----APLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKE 1592
            ...|...:...|.    |.|..| :.|...|...|.:...|.:...|    ..|....|.:|...
Human  1143 NADMETRRGAYLNALKIAKLLLT-AIGYGHVRAVAEACQPGVEGVNP----MTQINQVTHDQAVV 1202

  Fly  1593 RPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWR 1657
            ......:.|:|:                ||..::...:|...:|..|.    |.:..::.:
Human  1203 LQSALQSIPNPS----------------SECMLRNVSVRLAQQISDEA----SRYMPDICV---- 1243

  Fly  1658 HDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQH---QA 1719
               :.::.:|:|..|...||      |:..|:.      |:.:...:.::....::..:.   :|
Human  1244 ---IRAIQKIIWASGCGSLQ------LVFSPNE------EITKIYEKTNAGNEPDLEDEQVCCEA 1293

  Fly  1720 SSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTD---HCRWLQKF 1781
            .....|.|               .||..|::.|         ::...|.....|   ||.  .|.
Human  1294 LEVMTLCF---------------ALIPTALDAL---------SKEKAWQTFIIDLLLHCH--SKT 1332

  Fly  1782 ILEDPEPAVRRE----ICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLS 1842
            :.:     |.:|    :|.   |.|:|  |...|....||..::..........||
Human  1333 VRQ-----VAQEQFFLMCT---RCCMG--HRPLLFFITLLFTVLGSTARERAKHSG--------- 1378

  Fly  1843 EEGKDPYGPACRDYFWLLARL--------VDTLSPEMVAEEHID-IEMLCESISQSILTREYYEL 1898
                        |||.||..|        ::..:.|::....|| ::.:.:.:.::..|
Human  1379 ------------DYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGET------ 1425

  Fly  1899 RHGYQDDGLVGLLNLMSNLIKYDTTFK-----YTPKALSFIEQLI-GFLFDMPS----------- 1946
              |.::..|.|.|.:...|:.:.|:.|     ......:.|::|| .|:|...:
Human  1426 --GIEETILEGHLGVTKELLAFQTSEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRNGEL 1488

  Fly  1947 PADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGP-----KQPYPWDYWPRD 2006
            ||::..|.|.|..:..:.::|||.|..||..|...:...|...:..|.     :....|:|.|..
Human  1489 PAEQAIPVCGSPPTINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPV 1553

  Fly  2007 EGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAA-------------------- 2051
            ..|...|:|||.|.||||||.|.:|.|||:|..|..:|.:....:
Human  1554 GPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDP 1618

  Fly  2052 ----------------------RKH---GPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQP 2091
                                  ||.   | .|..||.:|.:|..|..:.|.||.|.:.:::..:|
Human  1619 RDDVFGYPQQFEDKPALSKTEDRKEYNIG-VLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEP 1682

  Fly  2092 LNTGEQKDMAEFFIDLVSKLEDMTPDLKH--LVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRC 2154
            :|..||.|..|||..||..|::....|.|  ::.::..||.::..:...|.|.....|.|.|:..
Human  1683 VNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNV 1747

  Fly  2155 QVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTM 2219
            .:.:.:||.:||::....|.|||.|.|.|.:|.|||...||...||||.:|.....|:.::....
Human  1748 DIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERE 1812

  Fly  2220 LKEKVNTHFSFPLRLNMCHY----VEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLD 2280
            ...|.|.:|.||..|:|..|    |.|........|.:..:|.|:         :::|.|.:|
Human  1813 CAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQSEQ---------SESETAGST-- 1866

  Fly  2281 DDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEV---KPFDPSQIAA 2342
                   :|.||||.||:|.|.||||||:|.:|.... ....||:.|:|.:|   |..|..::..
Human  1867 -------KYRLVGVLVHSGQASGGHYYSYIIQRNGGD-GERNRWYKFDDGDVTECKMDDDEEMKN 1923

  Fly  2343 ECFGGEMTSKTYDSVTEKYLDFSFEKTN---SAYMLFYERR----LPEHLQRRHSELLVTPTPSP 2400
            :|||||...:.:|.:.::   .|:.:..   :||:|||||.    ..:.|.|..|||.:|..|..
Human  1924 QCFGGEYMGEVFDHMMKR---MSYRRQKRWWNAYILFYERMDTIDQDDELIRYISELAITTRPHQ 1985

  Fly  2401 TVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEP 2465
            .:       .|:.:|.|
Human  1986 II-------MPSAIERS------------------------------------------------ 1995

  Fly  2466 EEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAA 2530
                :.||
Human  1996 ----VRKQ--------------------------------------------------------- 1999

  Fly  2531 QKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQI--CGHI-------PQSLISET- 2585
                                 |.||:.:|..:...||.||.::  |..:       ...|:.|.
Human  2000 ---------------------NVQFMHNRMQYSMEYFQFMKKLLTCNGVYLNPPPGQDHLLPEAE 2043

  Fly  2586 DVTCMAAKLSVSFFIETFIHAKE--KPTMVPWVELLTKQFNASQEACEWF-------LSHMSQEP 2641
            ::|.::.:|:..|...|..|.|:  :.:...|.:.|......|:....||       :|:...|
Human  2044 EITMISIQLAARFLFTTGFHTKKVVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSE- 2107

  Fly  2642 YWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLE-------VETDEDDKELIGQASCVT 2699
                 .|::||:..||..|.:|::.:        ||..|:       ..:.....:.....|...
Human  2108 -----YLLECPSAEVRGAFAKLIVFI--------AHFSLQDGPCPSPFASPGPSSQAYDNLSLSD 2159

  Fly  2700 RFIGSLISLL-----EHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQ 2759
            ..:.::::||     |||     |||.:||.|...::.:|..|...||::.|..:.:...|....
Human  2160 HLLRAVLNLLRREVSEHG-----RHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGP 2219

  Fly  2760 TDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKG 2824
            ...|                  |.:.|.|.|:.::.:.|: |......|::.|......:....|
Human  2220 GPPI------------------KYQYAELGKLYSVVSQLI-RCCNVSSRMQSSINGNPPLPNPFG 2265

  Fly  2825 FPFLYQQ----------------------IKDGINPHQTKHLIHALCRWDERLATQIIGMLFASV 2867
            .|.|.|.                      |:|..|..:|..|:...|..:.:.::.::..|...|
Human  2266 DPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQV 2330

  Fly  2868 T-KHTELCAPFFKLL--TLLTE 2886
            . .:|....|:..||  .||.|
Human  2331 AYSYTYELRPYLDLLLQILLIE 2352

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
puf	NP_001262923.1	peptidase_C19C	2013..2382	CDD:239124	129/429 (30%)
		UCH	2014..2377	CDD:278850	124/419 (30%)
		DUF3517	2723..>2877	CDD:288853	31/176 (18%)
USP9X	XP_005272732.1	peptidase_C19C	1560..1963	CDD:239124	129/425 (30%)
		UCH	1562..1958	CDD:278850	124/418 (30%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5077
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D625455at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.910