DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment puf and Usp24

DIOPT Version :9

Sequence 1:	NP_001262923.1	Gene:	puf / 42935	FlyBaseID:	FBgn0039214	Length:	3930	Species:	Drosophila melanogaster
Sequence 2:	XP_006503239.1	Gene:	Usp24 / 329908	MGIID:	1919936	Length:	2637	Species:	Mus musculus

Alignment Length:	2967	Identity:	625/2967 - (21%)
Similarity:	1038/2967 - (34%)	Gaps:	865/2967 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   348 KYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVE 412
            |...::|...|| |:::.:.:...|.|.|..|...::          ||.:.:  |.:|..:|..
Mouse   149 KCLLASTYLARL-GLSESDENCKRFMERCMPEAFKKL----------LTSSAV--HKWGTEIHEG 200

  Fly   413 IVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRM 477
            |.....:|:..:|  .::.::.|.:               .:|..|.....|....|..:   ||
Mouse   201 IYNMLMLLIELVA--ERMKQDPIPI---------------GLLGVLTMAFNPDNEYHFKN---RM 245

  Fly   478 DPKEHTEQSIY-------IASALTKQL----WTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEG 531
            ...:.....::       |:...|.|.    |..|.              .|.........:|:.
Mouse   246 KVSQRNWAEVFGEGNMFAISPVSTFQKEPHGWVVDL--------------VNKFGELGGFAAIQA 296

  Fly   532 S-NTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDP---TTEKGKQISPEDM 592
            . ::||..:||..:.:...|......|.|.:            :.:.||   ||       .:|:
Mouse   297 KLHSEDIELGAVSALVQPLGVCAEYLNSSVV------------QPMLDPVILTT-------IQDV 342

  Fly   593 AKV---DLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPH----- 649
            ..|   ||.:||:|:|      .|.|.:...|.::...:...|.:..|.....:.:..||
Mouse   343 RSVEEKDLKDKRLVSI------PELLSAIKLLCMRFQPALVTTVDALRLDILLRMLKSPHFSAKM 401

  Fly   650 -ELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAI 713
             .|.|:...:||...|.......|              :|.....||.::||.         .|:
Mouse   402 NSLKEVTKLIEDSTLSKSVKNAID--------------TDRLLDWLVENSVLS---------IAL 443

  Fly   714 ETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAA 778
            |.||::                       |.:.|.     .:.:.:|:.|:.:..::...:
Mouse   444 EGNIDQ-----------------------AQYCDR-----IKGIIELLGSKLSLDELTKIW---- 476

  Fly   779 AAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSD 843
                      .:|....|.|......:.||||....|.|    :|.       ..|:.....:::
Mouse   477 ----------KIQSGQSSTVIENIHTIIAAAAVKFNADQ----LNH-------LFVLIQKSWETE 520

  Fly   844 SD---VDLMDVVSG-GKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDL-----LQDD 899
            ||   ..|:.::.. |::      ::..:|||        |:.||       ||:|     |...
Mouse   521 SDRVRQKLLSLIGRIGRE------ARFEATSG--------KVLDV-------LWELAHLPTLPSS 564

  Fly   900 KIGQ-LGESLAL--------EAEKALATLLCF----------SMDRQLRTKFIEGCL-------- 937
            .|.| |.|.|.:        ||.|....:.|.          |:::..:..|.:|.|
Mouse   565 LIQQALEEHLTILSDAYAVKEAVKRSYIIKCIEDIKRPGEWSSLEKNKKDGFKQGGLTGDYVSLP 629

  Fly   938 -YNVANNRS----------VIVSLRLLPKLFASF--QQFRPSDTHSMTMWAERNHRMMQCFFNNI 989
             |.....||          |:.:||.|.::..||  |.::..| .|:....::|..:::....::
Mouse   630 GYTETKRRSSQLNNPQFVWVVPALRQLHEITRSFIKQTYQKQD-KSIIQDLKKNFEIVKLVTGSL 693

  Fly   990 RHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALW 1054
            . ...|.|..:....|......:.|: |:::..:...|:||:......    :..|...:...:|
Mouse   694 L-ACHRLAAAVAGPGGLTGLTLVDGR-YTYREYLEAHLKFLAFFLQEA----TLYLGWNRAKEIW 752

  Fly  1055 EWLAHDPECAD-----CYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQ 1114
            |.|....:..:     | |.|.   .||  ||.|..:..|.|:.:|:.:|...|.:|....||:.
Mouse   753 ECLVTGQDVCELDREMC-FEWF---TKG--QHDLESDVQQQLFKEKILKLESYEITMNGFNLFKT 811

  Fly  1115 LCSFARIAMAEYDNHSDQISA-SASAVGMYHLWKIALRAQSNDVSLAAIQ-YINMYYMGQQLRLE 1177
            ......:..........|:.. ....|||..:||||:.:...:::..||| .||..|:....||:
Mouse   812 FFENVNLCDHRLKRQGAQLYVEKLELVGMDFIWKIAMESPDEEIANEAIQLIINYSYINLNPRLK 876

  Fly  1178 KE-------FVSQCMENLVQA----------------------------ATALESIDDENALMRV 1207
            |:       |::.|...|..|                            ||:::|......|:.:
Mouse   877 KDSVSLHKKFIADCYTRLEAASSALGGPTLTHAVTRATKMLTATAMPTVATSVQSPYRSTKLVII 941

  Fly  1208 QRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMH 1272
            :|.|||...::.|....|:...   .|...|...|.:|        |.:.:......:...::.|
Mouse   942 ERLLLLAERYVITIEDFYSVPR---TILPHGASFHGHL--------LTLNVTYESTKDTFTVEAH 995

  Fly  1273 ACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQI 1337
            :.:.|..::.:::|       .|.:||       ..::|.|....||.:.|::.|...||.|.|:
Mouse   996 SNETIGSVRWKIAK-------QLCSPV-------DNIQIFTNDSLLTVNKDQKLLHQLGFSDEQV 1046

  Fly  1338 VYVSLGGRG-----------ARRKESNLEHPSMLPPPPKECLP-TVLLLQPKYFEKLFCLMQTLG 1390
            :.|...|.|           :....|:....|......::.|| .|:.|....|:.|:.|
Mouse  1047 LTVKTSGSGTPSGSSADSSTSSSSSSSGAFSSSYAMEQEKSLPGVVMALVCNVFDMLYQL----- 1106

  Fly  1391 DMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLAT 1455
                 |:...|:       ::.||..:|.::||:|.|.:|          |:|||:.|.::.|
Mouse  1107 -----ANLEEPR-------ITLRVRKLLLLIPTDPAIQEA----------LDQLDSLGRKKTL-- 1147

  Fly  1456 KRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRR 1520
                                          |:.|||                     :||.....
Mouse  1148 ------------------------------LSETSS---------------------QSSKSPSL 1161

  Fly  1521 NSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGE 1585
            :|.:|..|....:      |..|.....|..||.......|..||                |...
Mouse  1162 SSKQQHQPSASSI------LESLFRSFAPGMSTFRVLYNLEVLSS----------------KLMP 1204

  Fly  1586 TFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKE 1650
            |.:.:..|  .|:                   ..:.|.|:|.|||..:..:     :|:.:.|.|
Mouse  1205 TADDDMAR--SCA-------------------KSFCENFLKAGGLSLVVNV-----MQRDSIPSE 1243

  Fly  1651 LALNEWRHD----CLASLLRILWLLG-------FEELQSADAHVLMSRPH--------------- 1689
            :.. |.|..    || .|.|.| |:|       .|:|.......|.|||.
Mouse  1244 VDY-ETRQGVYSICL-QLARFL-LVGQTMPTSLDEDLTKDGIEALSSRPFRNVSRQTSRQMSLCG 1305

  Fly  1690 ---------------------------PFMLQLMEVPQ------CLTRLSSILN----DEVHQQH 1717
                                       ...:|.||...      |..|||....    |.|....
Mouse  1306 TPEKSSYRQLSVSDRSSIRVEEIIPAARVAIQTMEASDFTATVACFMRLSWAAAAGRLDLVGSSQ 1370

  Fly  1718 QASSANPLVFPYQFQH-------------------LRTGFWGRAQLIQF--------AMNILVSF 1755
            ....:|.| ||...:.                   |..|...|.|.:..        |:::||:.
Mouse  1371 PIKESNSL-FPAGIRSRLSSSGSNCSSSSEGEPAALHAGICVRQQSVSTKDALIAGEALSLLVTC 1434

  Fly  1756 VHASAEARRLLWA-PTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSY-------RLL 1812
            :...::.....:: |...|   ::...:|..|...:||..|..||.:...:..::       :.|
Mouse  1435 LQLRSQQLASFYSLPCVAD---FIIDILLGSPSAEIRRVACDQLYTLSQTDTSAHPEVQKPNQFL 1496

  Fly  1813 LAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTL---------- 1867
            |..:   |.|.|||....|......|.|||:         |.:||.|..:|:|.|
Mouse  1497 LGVI---LTAQLPLWSPTSIMRGVNQRLLSQ---------CMEYFDLRCQLLDDLTTSEMDQLRI 1549

  Fly  1868 SPEMVAEEHI------------DIEMLCE-SISQSILTREYYELRHGYQDDGLVGLLNLM----- 1914
            ||..:.|:.|            |    || |.:.:||...:..|        :..||:|.
Mouse  1550 SPATMLEDEITWLDNFEPNRTAD----CETSEADNILLAGHLRL--------IKTLLSLCGAEKE 1602

  Fly  1915 ---SNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQ-------KPKCKSASSRASAYDLLV 1969
               |:|||        |....|:.:....:.:..|||...       .|||.:.:||.:||::||
Mouse  1603 MLGSSLIK--------PLLDDFLFRASRIIVNSHSPASSAAISQQDFHPKCSTVNSRLAAYEVLV 1659

  Fly  1970 ELCRGCATNYAYLHGRLLAQHKSGPKQPYP-----WDYWPRDEGRAECGYVGLTNLGATCYMASC 2029
            .|.....:|...:...||:.|    .||.|     :||.|..:.|:..|:|||.|.||||||.:.
Mouse  1660 MLADSSPSNLQIITKELLSMH----HQPDPALTKEFDYLPPVDSRSSSGFVGLRNGGATCYMNAV 1720

  Fly  2030 VQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNT 2094
            .|.|||.|....::|.|..:..........::|.:|.:|:||:.:.|.|.:|.::::|.::.|..
Mouse  1721 FQQLYMQPGLPESLLSVDDDTDNPDDSVFYQVQSLFGHLMESKLQYYVPENFWKIFKMWNKELYV 1785

  Fly  2095 GEQKDMAEFFIDLVSKLEDMTPDL--KHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVA 2157
            .||:|..|||..|:.::::....:  :.:.|..|.|..|:..:..||.|.....|.|..:...|.
Mouse  1786 REQQDAYEFFTSLIDQMDEYLKKMGREQIFKNTFQGIYSDQKICKDCPHRYEREEAFMALNLGVT 1850

  Fly  2158 DMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKE 2222
            ..::|:.|||:....:.|||.|.|.|.:|.:|....||.|.|.||.:|..:.||:.|:..:....
Mouse  1851 SCQSLEISLDQFVRGEVLEGSNAYYCEKCKEKRITVKRTCIKSLPSVLVIHLMRFGFDWESGRSI 1915

  Fly  2223 KVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECY 2287
            |.:....||..|||..|....:..|....|.....:   ..|..|.|:..:|...|        .
Mouse  1916 KYDEQIRFPWMLNMEPYTVAGMARQDSSSEVGENGR---NMDQGGGGSPRKKVALT--------E 1969

  Fly  2288 EYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFD--PSQIAAECFGGEMT 2350
            .||||||.||:|.|..||||||||:|....   ..:|:.|||..::.||  ...:..||||||..
Mouse  1970 NYELVGVIVHSGQAHAGHYYSFIKDRRGCG---KGKWYKFNDTVIEEFDLNDETLEYECFGGEYR 2031

  Fly  2351 SKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKME 2415
            .|.||. |..|.|......| ||||||:|              |:...||.:.:||....
Mouse  2032 PKVYDQ-TNPYTDVRRRYWN-AYMLFYQR--------------VSDQNSPVLPKKSRVSV----- 2075

  Fly  2416 TSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEK 2480
                       |..|.|:.           |...|:..||    |.|..|
Mouse  2076 -----------VRQEAEDL-----------SLSAPSSPEI----SPQSSP--------------- 2099

  Fly  2481 SETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELE 2545
                 :|                    |:.||:..|..:     ...|..:|..||...:...:.
Mouse  2100 -----RP--------------------HRPNNDRLSILT-----KLVKKGEKKGLFVEKMPARIY 2134

  Fly  2546 DWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMA---AKLSVSFFIETFIHAK 2607
            ..:..:|.:|:::|:::...||:|:..:.. :..:.:......|||   .:|::.|..:|::..|
Mouse  2135 QMVRDENLKFMKNRDVYSSDYFSFVLSLAS-LNATKLKHPYYPCMAKVSLQLAIQFLFQTYLRTK 2198

  Fly  2608 EKPTM--VPWVELLTKQFNASQEACEWFLSH-MSQEPYWPVQV-LIQCPNQMVRQMFQRLVIHVI 2668
            :|..:  ..|:..:....:.|.:||:|.:.: :|.|....|:| |::|   .||::  |:.:..|
Mouse  2199 KKLRVDTEEWIATIEALLSKSLDACQWLVEYFISSEGRELVKVFLLEC---SVREV--RVAVATI 2258

  Fly  2669 QQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEF-SR 2732
            .:.....|..|       .||     ...:.:.:..|::||:.....|.::.::||.|...| .:
Mouse  2259 LEKTLDSALFY-------QDK-----LKSLHQLLEVLLALLDKDVPENCKNCAQYFSLFNTFVQK 2311

  Fly  2733 MGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDN---SSEEALSVEKMRPASLDKMIAL 2794
            .|......|||...|:.::.|.||        :|..|...   ||.:|...     .:|...:||
Mouse  2312 QGIRAGDLLLRHSALRHMISFLLG--------VSRQNSQIRRWSSAQAREF-----GNLHNTVAL 2363

  Fly  2795 CASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQI 2859
               ||..|..:..| .::|..|.            |:....:.|......:|          .::
Mouse  2364 ---LVLHSDVSSQR-NVAPGIFK------------QRPPISVAPSSPLLPLH----------EEV 2402

  Fly  2860 IGMLFASVTKHTELCAPF-FKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYC--PQSVLDWLS 2921
            ..:||.|..|      |: .:::..|.|..|.           |..:.:...||  ......:..
Mouse  2403 EALLFLSEGK------PYLLEVMFALRELTGS-----------LLALMEMVVYCCFCNEHFSFTM 2450

  Fly  2922 LQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLAL 2986
            |...||::..|    ...:....|.|.|:          :||...|.|..|..|...:::.||||
Mouse  2451 LHFIKNQLETA----PPHELKNTFQLLHE----------VLVIEDPIQVERVKFVFETENGLLAL 2501

  Fly  2987 NPHSYRDINSDAQAVLHQVITLLLRLLR---PARVYADIGAH 3025
            ..||    |....:..:|.:..|:.|.:   .|:.|....:|
Mouse  2502 MHHS----NHVDSSRCYQCVKFLVTLAQKCPAAKEYFKENSH 2539

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
puf	NP_001262923.1	peptidase_C19C	2013..2382	CDD:239124	126/372 (34%)
		UCH	2014..2377	CDD:278850	122/366 (33%)
		DUF3517	2723..>2877	CDD:288853	33/157 (21%)
Usp24	XP_006503239.1	UBA_UBP24	6..42	CDD:270472
		Ubl_UBP24	972..1050	CDD:340585	19/99 (19%)
		peptidase_C19C	1704..2061	CDD:239124	127/386 (33%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5077
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D625455at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820