Protein Alignment puf and Usp28

DIOPT Version :9

• Sequence 1: NP_001262923.1
• Gene: puf / 42935
• FlyBaseID: FBgn0039214
• Length: 3930
• Species: Drosophila melanogaster
• Sequence 2: XP_006243068.1
• Gene: Usp28 / 315639
• RGDID: 1311555
• Length: 1143
• Species: Rattus norvegicus
• Alignment Length: 1273
• Identity: 251/1273 - (19%)
• Similarity: 408/1273 - (32%)
• Gaps: 478/1273 - (37%)

  Fly  1804 GNAHSYRLLLAPL-----------LHKLIALLPLAEQMSSGN--QHTQFLLSEEGKDPYGPACRD 1855
            |:..|.::||..|           ||:       |.:.|:|:  |....|..:..|:|
  Rat    16 GHGSSCQMLLNQLREITGIQDPSFLHE-------ALKASNGDITQAVSLLTDQRVKEP------- 66

  Fly  1856 YFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDD--GLVGLLNLMSNLI 1918
                        |.:..|.|..::|   ||.:...|..:..:|.|..:||  ..:.|..|.|..|
  Rat    67 ------------SHDTAATEPSEVE---ESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNI 116

  Fly  1919 KYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLH 1983
            :.|:      :.|:.|.:        .:.|:.::.|.|.              |.....|:
  Rat   117 QTDS------RDLNRIHE--------ANSAETKRSKRKR--------------CEVWGENH---- 149

  Fly  1984 GRLLAQHKSGPKQPYPW---DYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVL- 2044
                        .|..|   |.||          |||.|:|.||:.::.:|.|:.:|:.|..||
  Rat   150 ------------NPNNWRRVDGWP----------VGLKNVGNTCWFSAVIQSLFQLPEFRRLVLS 192

  Fly  2045 -RVPPNA---ARKHGPT-----LLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDM 2100
             .:|.|.   .|.|...     :.|||.:||.||.|.||..:|.:...:.:...:. :..:|:|:
  Rat   193 YSLPQNILENCRSHTEKRNIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRS-SEEQQQDV 256

  Fly  2101 AEFFIDLVSKLED---------MTPDLK--HLVKRLFCGS-LSNNVVSLDCGHVSRTAEDFYTVR 2153
            :||...|:..|||         ..|..|  :.:.:||.|: |:..|..   |......|.|....
  Rat   257 SEFTHKLLDWLEDAFQLAVNVNSNPRTKSENPMVQLFYGTFLTEGVRE---GKPFCNNETFGQYP 318

  Fly  2154 CQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVT 2218
            .||....||.|.|:...|    |||.....|.  :.|:..:...|.|||.:|.|...|:.||...
  Rat   319 LQVNGYHNLDECLEGAMV----EGDIALLPSD--RSVKYGQERWFTKLPPVLTFELSRFEFNQSL 377

  Fly  2219 MLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEK----------------------- 2260
            ...||::....||..:.|..|:.|:....:.|.|..|:.||:
  Rat   378 GQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQQKLERYVKYGSGPSRFPL 442

  Fly  2261 ------------------------------------------------------EGADGS----G 2267
                                                                  :.|:||    .
  Rat   443 PDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKSCSQNAEGSFSSPE 507

  Fly  2268 DGNDNEK--------------------AEATLDDD------------------------------ 2282
            |...|.:                    |..|:.|:
  Rat   508 DALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQDLKNCISST 572

  Fly  2283 ---IEECY--------EYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFD 2336
               ||:.|        .|.|..|.||.|.|..|||:::|..:.:      :.|..:||..|....
  Rat   573 TQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPR------QIWLKYNDISVTESS 631

  Fly  2337 PSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFY-ERRLPEHLQRRHSELLVTPTPSP 2400
            ..::..:.:||                   .:..|||.|.| ..:|| |.....:.
  Rat   632 WEELERDSYGG-------------------LRNVSAYCLMYINDKLP-HCSAEAAH--------- 667

  Fly  2401 TVEEKSEAEEPTKMETSSSEIKADVD-----VEVEVEEKDKEKPAQ----------------TDT 2444
                 .|:::..::|..|.|::..:.     .|.||||.::|:..:                |..
  Rat   668 -----GESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEEQSCKIPQMESSPNSSSQDFSTSQ 727

  Fly  2445 ESKETP--------------AKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVST 2495
            ||....              |||:.|...:......||. ..::...|.|....|||.|   .:.
  Rat   728 ESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKS-GVEAALSEHKEAEPEKPQE---TNL 788

  Fly  2496 EEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQ--- 2557
            .|:.:..|...:.:.....||:.|..:.||..         |.|:..:.:.:   |....|.
  Rat   789 AEQSEQAAEVKDTEAAAPPNSEVSEVEIPSVG---------RILVRSDADGY---DEEVMLSPAM 841

  Fly  2558 --------------DRNIFE-------HTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIE 2601
                          ||:..|       |..::.::|:....|   .|.:|....  .:.|.||
  Rat   842 QGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETP---TSHSDPRLQ--HVLVYFF-- 899

  Fly  2602 TFIHAKEKPTMVPWVE-LLTKQF---NASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQ---- 2658
                ..|.|..|  || .|.:||   |.|.:.....:..::|.....:.     |..|..:
  Rat   900 ----QNEAPKRV--VERTLLEQFADRNLSYDERSISIMKVAQAKLMEIG-----PEDMNMEEYKR 953

  Fly  2659 ------MFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANL 2717
                  :|:::.::::..|.......|.|.                       :|.|.:..::|.
  Rat   954 WHEDYSLFRKVSVYLLTGLELFQKGKYQEA-----------------------LSYLVYAYQSNA 995

  Fly  2718 RHLSEYFGLLCEFSRMGDEE---AMY----LLRIGV-LKSLVDFYLGHKQTDSIDISSD------ 2768
                   |||.:..|.|.:|   |:|    ||.:.. ..||.:....|..|:.|::.::
  Rat   996 -------GLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCI 1053

  Fly  2769 ----NEDNSSEEALSVEKMRP---ASLDKMIA----LC 2795
                |.|.|.::..::|.||.   :.|.|.||    ||
  Rat  1054 HLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLC 1091

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
puf	NP_001262923.1	peptidase_C19C	2013..2382	CDD:239124	112/533 (21%)
		UCH	2014..2377	CDD:278850	111/526 (21%)
		DUF3517	2723..>2877	CDD:288853	27/98 (28%)
Usp28	XP_006243068.1	UBA_UBP28	23..64	CDD:270540	10/47 (21%)
		UCH	161..>399	CDD:278850	73/257 (28%)
		Peptidase_C19I	163..654	CDD:239130	110/525 (21%)
		Peptidase_C19	163..>271	CDD:271592	35/108 (32%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5077
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810