Protein Alignment puf and Usp24

DIOPT Version :9

• Sequence 1: NP_001262923.1
• Gene: puf / 42935
• FlyBaseID: FBgn0039214
• Length: 3930
• Species: Drosophila melanogaster
• Sequence 2: NP_001258135.1
• Gene: Usp24 / 313427
• RGDID: 1306799
• Length: 2617
• Species: Rattus norvegicus
• Alignment Length: 3092
• Identity: 645/3092 - (20%)
• Similarity: 1105/3092 - (35%)
• Gaps: 863/3092 - (27%)

  Fly   220 EEAHHLALIC-------------DLHDNDL--PIYLLRNVSLFCNSGGFGAMSLCFEHPDL---- 265
            ||.|...|:|             .|..||:  .:.||.|.....:.||:..|......|.|
  Rat     5 EEQHMTTLLCMGFSDPATIRKALRLAKNDINEAVALLTNERPGLDYGGYEPMDSGGPSPGLGGGP 69

  Fly   266 ------------------------PVSTAHSMTAAVSNVKLWLNYHCN--------TQLFVPLRS 298
                                    |....|.:..|..|.:   |.:|:        |.|: .|.|
  Rat    70 RGDGGGEGSGPSRGGSTGGGGGFDPPPAYHEVVDAEKNDE---NGNCSGEGVEFPTTNLY-ELES 130

  Fly   299 RILQYMCKLSDQSLRSAATRAMADFVWS--SMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAG 361
            |:      |:|.              ||  ..|:          |.|.   |...::|...|| |
  Rat   131 RV------LTDH--------------WSIPYKRE----------ESLG---KCLLASTYLARL-G 161

  Fly   362 MAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAV 426
            :::.:.:...|.|.|..|...::          ||.:.:  |.:|..:|..|.....:|:..:|
  Rat   162 LSESDENCKRFMERCMPEAFKKL----------LTSSAV--HKWGTEIHEGIYNMLMLLIELVA- 213

  Fly   427 ENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIY--- 488
             .::.::.|.:               .:|..|.....|....|..:   ||...:.....::
  Rat   214 -ERMKQDPIPI---------------GLLGVLTMAFNPDNEYHFKN---RMKVSQRNWAEVFGEG 259

  Fly   489 ----IASALTKQL----WTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGS-NTEDDHVGADDS 544
                ::...|.|.    |..|.              .|.........:|:.. ::||..:||..:
  Rat   260 NMFAVSPVSTFQKEPHGWVVDL--------------VNKFGELGGFAAIQAKLHSEDIELGAVSA 310

  Fly   545 SIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDP---TTEKGKQISPEDMAKV---DLVNKRIV 603
            .:...|......|.|.:            :.:.||   ||       .:|:..|   ||.:||:|
  Rat   311 LVQPLGVCAEYLNSSVV------------QPMLDPVILTT-------IQDVRSVEEKDLKDKRLV 356

  Fly   604 NIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPH------ELVEIWDGVEDVP 662
            :|.:..|:.:.|..|...:|.......:.....|..|:      ||      .|.|:...:||..
  Rat   357 SIPELLSAIKLLCMRFQPDLVTIVDDLRLDILLRMLKS------PHFSAKMNSLKEVTKLIEDST 415

  Fly   663 SSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAEND 727
            .|.......|              :|.....||.::||.         .|:|.||::
  Rat   416 LSKSVKNAID--------------TDRLLDWLVENSVLS---------IALEGNIDQ-------- 449

  Fly   728 GSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQK 792
                           |.:.|.     .:.:.:|:.|:.:..::...:              .:|.
  Rat   450 ---------------AQYCDR-----IKGIIELLGSKLSLDELTKIW--------------KIQS 480

  Fly   793 SGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSD---VDLMDVVSG 854
            ...|.|......:.||||....|.|    :|.       ..|:.....::::|   ..|:.::..
  Rat   481 GQSSTVIENIHTIIAAAAVKFNADQ----LNH-------LFVLIQKSWETETDRVRQKLLSLIGR 534

  Fly   855 -GKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALAT 918
             |::      ::..:|||        |:.||       ||:|..   :..|..||..:|.:...|
  Rat   535 IGRE------ARFEATSG--------KVLDV-------LWELAH---LPTLPSSLIQQALEEHLT 575

  Fly   919 LL--CFSMDRQLRTKFIEGCLYNVA---------------------NNRS---VIVSLRLLPKLF 957
            :|  .:::...::..:|..|:.::.                     ||..   |:.:||.|.::.
  Rat   576 ILSDAYAVKEAVKRSYIIKCIEDIKRPGEWSGLEKNKKDGFKSSQHNNPQFVWVVPALRQLHEIT 640

  Fly   958 ASF--QQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHK 1020
            .||  |.::..| .|:....::|..:::....::. ...|.|..:....|......:.|: |:::
  Rat   641 RSFIKQTYQKQD-KSIIQDLKKNFEIVKLVTGSLL-ACHRLAAAVAGPGGLTGLTLVDGR-YTYR 702

  Fly  1021 TQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECAD-----CYFSWLQAQAKGGDQ 1080
            ..:...|:||:......    :..|...:...:||.|....:..:     | |.|.   .||  |
  Rat   703 EYLEAHLKFLAFFLQEA----TLYLGWNRAKEIWECLVTGQDVCELDREMC-FEWF---TKG--Q 757

  Fly  1081 HALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISA-SASAVGMYH 1144
            |.|..:..|.|:.:|:.:|...|.:|....||:.......:..........|:.. ....|||..
  Rat   758 HDLESDVQQQLFKEKILKLESYEITMNGFNLFKTFFENVNLCDHRLKRQGAQLYVEKLELVGMDF 822

  Fly  1145 LWKIALRAQSNDVSLAAIQ-YINMYYMGQQLRLEKE-------FVSQCMENLVQA---------- 1191
            :||||:.:...:::..||| .||..|:....||:|:       |::.|...|..|
  Rat   823 IWKIAMESPDEEIANEAIQLIINYSYINLNPRLKKDSVSLHKKFIADCYTRLEAASSALGGPTLT 887

  Fly  1192 ------------------ATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKG 1238
                              ||:::|......|:.::|.|||...::.|....|:...   .|...|
  Rat   888 HAVTRATKMLTATAMPTVATSVQSPYRSTKLVIIERLLLLAERYVITIEDFYSVPR---TILPHG 949

  Fly  1239 IGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLL 1303
            ...|.:|        |.:.:......:...::.|:.:.|..::.:::|       .|.:||
  Rat   950 ASFHGHL--------LTLNVTYESTKDTFTVEAHSNETIGSVRWKIAK-------QLCSPV---- 995

  Fly  1304 LSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRG-----------ARRKESNLEHP 1357
               ..::|.|....||.:.|::.|...||.|.|::.|...|.|           :....|:....
  Rat   996 ---DNIQIFTNDSLLTVNKDQKLLHQLGFSDEQVLTVKTSGSGTPSGSSADSSTSSSSSSSGVFS 1057

  Fly  1358 SMLPPPPKECLP-TVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAML 1421
            |......::.|| .|:.|....|:.|:.|          |:...|:       ::.||..:|.::
  Rat  1058 SSYAMEQEKSLPGVVMALVCNVFDMLYQL----------ANLEEPR-------ITLRVRKLLLLI 1105

  Fly  1422 PTNPHILDAFKSLVTDLSELEQLDAGGEEE--------------QLATKRKQ-------IKQKFR 1465
            ||:|.|.:|          |:|||:.|.::              .|::|::|       ::..||
  Rat  1106 PTDPAIQEA----------LDQLDSLGRKKTLLSETSSPSSKSPSLSSKQQQQPSASSILESLFR 1160

  Fly  1466 DLLDPNNLQKFMYSLHIVESLAL--------TSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNS 1522
            ......:..:.:|:|.::.|..:        .|..:......:..|.....|.|      .:|:|
  Rat  1161 SFAPGMSTFRVLYNLEVLSSKLMPTADDDMARSCAKSFCENFLKAGGLSLVVNV------MQRDS 1219

  Fly  1523 NEQPPPPPPEVKMSKEQ----LC-ELEAPLT--PTPSTGLQDVET----EASSSSGGDKENQPKQ 1576
                  .|.||.....|    :| :|...|.  .|..|.|.:..|    ||.||       :|.:
  Rat  1220 ------IPSEVDYETRQGVYSICLQLARFLLVGQTMPTSLDEDLTKDGIEALSS-------RPFR 1271

  Fly  1577 HSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQ 1641
            :..||    |..|...    |.||...:  ...|||.:|...:             :.||....:
  Rat  1272 NVSRQ----TSRQMSL----CGTPEKSS--YRQLSVSDRSSIR-------------VEEIIPAAR 1313

  Fly  1642 LQ-QSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRL 1705
            :. |:....:....      :|..:|:.|        :|.|.         .|.|:...|.:...
  Rat  1314 VAIQTMEANDFTAT------VACFMRLSW--------AAAAG---------RLDLVGSSQPIKES 1355

  Fly  1706 SSILNDEVHQQHQASSAN--------PLVFPYQFQHLRTGFWGRAQLIQF--------AMNILVS 1754
            :|:....:..:..:|.:|        |..       |..|...|.|.:..        |:::||:
  Rat  1356 NSLFPAGIRNRLSSSGSNCSSSSEGEPAA-------LHAGICVRQQSVSTKDALIAGEALSLLVT 1413

  Fly  1755 FVHASAEARRLLWA-PTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSY-------RL 1811
            .:...::.....:: |...|   ::...:|..|...:||..|..||.:...:..::       :.
  Rat  1414 CLQLRSQQLASFYSLPCVAD---FIIDILLGSPSAEIRRVACDQLYTLSQTDTSAHPEVQKPNQF 1475

  Fly  1812 LLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTL--------- 1867
            ||..:   |.|.|||....|......|.|||:         |.:||.|..:|:|.|
  Rat  1476 LLGVI---LTAQLPLWSPTSIMRGVNQRLLSQ---------CMEYFDLRCQLLDDLTTSEMEQLR 1528

  Fly  1868 -SPEMVAEEHI--------DIEMLCE-SISQSILTREYYELRHGYQDDGLVGLLNLM-------- 1914
             ||..:.|:.:        :....|| |.:.:||...:..|        :..||:|.
  Rat  1529 VSPAAMLEDEVTWLDNFEPNRTAECETSEADNILLAGHLRL--------IKTLLSLCGAEKEVLG 1585

  Fly  1915 SNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQ-------KPKCKSASSRASAYDLLVELC 1972
            |:|||        |....|:.:....:.:..|||...       .|||.:.:||.:||::||.|.
  Rat  1586 SSLIK--------PLLDDFLFRASRIILNSHSPAGSAAISQQDFHPKCSTVNSRLAAYEVLVMLA 1642

  Fly  1973 RGCATNYAYLHGRLLAQHKSGPKQPYP-----WDYWPRDEGRAECGYVGLTNLGATCYMASCVQH 2032
            ....:|...:...||:.|    .||.|     :||.|..:.|:..|:|||.|.||||||.:..|.
  Rat  1643 DSSPSNLQIITKELLSMH----HQPDPALTKEFDYLPPVDSRSISGFVGLRNGGATCYMNAVFQQ 1703

  Fly  2033 LYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQ 2097
            |||.|....::|.|..:..........::|.:|.:|:||:.:.|.|.:|.::::|.::.|...||
  Rat  1704 LYMQPGLPESLLSVDDDTDNPDDSVFYQVQSLFGHLMESKLQYYVPENFWKIFKMWNKELYVREQ 1768

  Fly  2098 KDMAEFFIDLVSKLEDMTPDL--KHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMR 2160
            :|..|||..|:.::::....:  :.:.|..|.|..|:..:..||.|.....|.|..:...|...:
  Rat  1769 QDAYEFFTSLIDQMDEYLKKMGREQIFKNTFQGIYSDQKICKDCPHRYEREEAFMALNLGVTSCQ 1833

  Fly  2161 NLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVN 2225
            :|:.|||:....:.|||.|.|.|.:|.:|....||.|.|.||.:|..:.||:.|:..:....|.:
  Rat  1834 SLEISLDQFVRGEVLEGSNAYYCEKCKEKRITVKRTCIKSLPSVLVIHLMRFGFDWESGRSIKYD 1898

  Fly  2226 THFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYE 2290
            ....||..|||..|....:..|....|.....:   ..|..|.|:..:|...|        ..||
  Rat  1899 EQIRFPWMLNMEPYTVAGMARQDSSSEVGENGR---NMDQGGGGSPRKKVALT--------ENYE 1952

  Fly  2291 LVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFD--PSQIAAECFGGEMTSKT 2353
            ||||.||:|.|..||||||||:|....   ..:|:.|||..::.||  ...:..||||||...|.
  Rat  1953 LVGVIVHSGQAHAGHYYSFIKDRRGCG---KGKWYKFNDTVIEEFDLNDETLEYECFGGEYRPKV 2014

  Fly  2354 YDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSS 2418
            ||. |..|.|......| ||||||:|              |:...||.:.:||....
  Rat  2015 YDQ-TNPYPDVRRRYWN-AYMLFYQR--------------VSDQNSPVLPKKSRVSV-------- 2055

  Fly  2419 SEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSET 2483
                    |..|.|:.           |...|:..||    |.|..|
  Rat  2056 --------VRQEAEDL-----------SLSAPSSPEI----SPQSSP------------------ 2079

  Fly  2484 DEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWI 2548
              :|                    |:.||:..|..:     ...|..:|..||...:...:...:
  Rat  2080 --RP--------------------HRPNNDRLSILT-----KLVKKGEKKGLFVEKMPARIYQMV 2117

  Fly  2549 WQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMA---AKLSVSFFIETFIHAKEKP 2610
            ..:|.:|:::|:::...||:|:..:.. :..:.:......|||   .:|:|.|..:|::..|:|.
  Rat  2118 RDENLKFMKNRDVYSSDYFSFVLSLAS-LNATKLKHPHYPCMAKVSLQLAVQFLFQTYLRTKKKL 2181

  Fly  2611 TM--VPWVELLTKQFNASQEACEWFLSH-MSQEPYWPVQV-LIQCPNQMVRQMFQRLVIHVIQQL 2671
            .:  ..|:..:....:.|.:||:|.:.: :|.|....|:| |::|   .||::  |:.:..|.:.
  Rat  2182 RVDTEEWIATIEALLSKSLDACQWLVEYFISSEGRELVKVFLLEC---SVREV--RVAVATILEK 2241

  Fly  2672 RASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEF-SRMGD 2735
            ....|..|       .||     ...:.:.:..|::||:.....|.::.::||.|...| .:.|.
  Rat  2242 TLDSALFY-------QDK-----LKSLHQLLEVLLALLDKDVPENCKNCAQYFSLFNTFVQKQGI 2294

  Fly  2736 EEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRP-ASLDKMIALCASLV 2799
            .....|||...|:.::.|.||..|           .||.....|..:.|. .:|...:||.....
  Rat  2295 RAGDLLLRHSALRHMISFLLGVSQ-----------QNSQIRRWSSAQAREFGNLHNTVALLVLHT 2348

  Fly  2800 ERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLF 2864
            :.|         |.::.:        |.:::| :..|:...:..|:        .|..::..:||
  Rat  2349 DVS---------SQRNVA--------PGIFKQ-RPPISVAPSSPLL--------PLHEEVEALLF 2387

  Fly  2865 ASVTKHTELCAPF-FKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYC--PQSVLDWLSLQATK 2926
            .|..|      |: .:::..|.|..|.           |..:.:...||  ......:..|...|
  Rat  2388 LSEGK------PYLLEVMFALRELTGS-----------LLALMEMVVYCCFCNEHFSFTMLHFIK 2435

  Fly  2927 NKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSY 2991
            |::..|    ...:....|.|.|:          :||...|.|..|..|...:::.||||..||
  Rat  2436 NQLETA----PPHELKNTFQLLHE----------ILVMEDPIQVERVKFVFETENGLLALMHHS- 2485

  Fly  2992 RDINSDAQAVLHQVITLLLRLLR---PARVYADIGAH 3025
               |....:..:|.:..|:.|.:   .|:.|....:|
  Rat  2486 ---NHVDSSRCYQCVKFLVTLAQKCPAAKEYFKENSH 2519

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
puf	NP_001262923.1	peptidase_C19C	2013..2382	CDD:239124	126/372 (34%)
		UCH	2014..2377	CDD:278850	122/366 (33%)
		DUF3517	2723..>2877	CDD:288853	31/155 (20%)
Usp24	NP_001258135.1	UBA_UBP24	6..42	CDD:270472	8/35 (23%)
		peptidase_C19C	1684..2041	CDD:239124	127/386 (33%)
		UCH	1686..2036	CDD:278850	122/365 (33%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5077
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D625455at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910