Protein Alignment puf and Usp9x

DIOPT Version :9

• Sequence 1: NP_001262923.1
• Gene: puf / 42935
• FlyBaseID: FBgn0039214
• Length: 3930
• Species: Drosophila melanogaster
• Sequence 2: XP_006527649.1
• Gene: Usp9x / 22284
• MGIID: 894681
• Length: 2575
• Species: Mus musculus
• Alignment Length: 3149
• Identity: 573/3149 - (18%)
• Similarity: 1011/3149 - (32%)
• Gaps: 1183/3149 - (37%)

  Fly   102 QQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTAN 166
            |.|...|:::.|:...:.|:| ||..|           :.||.||.|   |.:....|..|.|
Mouse    23 QSQPPLQQNQTSSPDSSNENS-PATPP-----------DEQGQGDAP---PQIEDEEPAFPHT-- 70

  Fly   167 PQHKSHLQYTEEPWILPEVEK--LLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQ----------- 218
            ...|.........|::|.:.|  |.||:.....|:       |.|:..:.:..|
Mouse    71 DLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLS-------KKGLDVKSEACQRFFRDGLTISF 128

  Fly   219 ----AEEA----------------HHLALIC--DLHDNDLPIYLL-------------------- 241
                .:||                |.|..:|  .|..:..|:..|
Mouse   129 TKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLAQDWFPLLELLAMALNPHCKFHIYNGTRPC 193

  Fly   242 RNVS---------LFCNS--------------GGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKL- 282
            .:||         ||..|              ..||.::      ...:.....:..:..||::
Mouse   194 ESVSSSVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLN------GFQILHDRFINGSALNVQII 252

  Fly   283 ------------WLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVA 335
                        :|..|...:.|:|:...:.|::..|:|:.|:..|..       .:..|.|.:.
Mouse   253 AALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEAKN-------EAKNDALSMI 310

  Fly   336 VNFDTEGLALAFKYFTSTT---------LTMRLAGMAQINAHINLFNEICTTETVNEVE------ 385
            :. ..:.||........|.         :.:||..::..|..:|..||:  .:.::.|.
Mouse   311 IK-SLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEV--NKVISSVSYYTHRH 372

  Fly   386 --------LFGQRIANWLTENHIVQHLFGPNLH-VEIVKQAHVLLNFLAVENQISEEDIKLIWQA 441
                    |..:|:|.|:.:|:|:..:...:|| .:.|::...:|.|:..|..::.:|:..||.|
Mouse   373 GSSEDEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAA 437

  Fly   442 TQLKH--CSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSR 504
            ...||  ..|.:.|:|..|..:.:|....||:...                    |..||..:.:
Mouse   438 QAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCF--------------------KASWTNASKK 482

  Fly   505 SQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQ 569
            .:..|::                 :.....|||.                       |||
Mouse   483 QREKLLE-----------------LIRRLAEDDK-----------------------DGV----- 502

  Fly   570 ARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSN 634
                                  ||      .:::|::.|.:..::
Mouse   503 ----------------------MA------HKVLNLLWNLAHSDD-------------------- 519

  Fly   635 KRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAV 699
                        :|.:::::                        .|:...:..|.:.||   |..
Mouse   520 ------------VPVDIMDL------------------------ALSAHIKILDYSCSQ---DRD 545

  Fly   700 LKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSR 764
            .:.:|....||..:.||                              |:.|.|..:::.::
Mouse   546 TQKIQWIDRFIEELRTN------------------------------DKWVIPALKQIREI---- 576

  Fly   765 ANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVA 829
              ||                                          ..|.|.|.|
Mouse   577 --CS------------------------------------------LFGEAPQNL---------- 587

  Fly   830 AAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWD 894
                                            .:|:.|.:..|..|
Mouse   588 --------------------------------SSSRFSQSQRSPHV------------------- 601

  Fly   895 LLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFAS 959
            ..:.|.|.|      |:...||.||                    ||.|             .|:
Mouse   602 FYRHDLINQ------LQHNHALVTL--------------------VAEN-------------LAT 627

  Fly   960 FQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVS 1024
            :.:       ||.|:...|                        ::.:.|..:||.: |||..:|.
Mouse   628 YME-------SMRMYGRDN------------------------EDYDPQTVRLGSR-YSHVQEVQ 660

  Fly  1025 VRLQFLSSIFSTVGSPKSFRLTL--EQLDALWEWLAH------DPECADCYFSWLQAQAKGGDQH 1081
            .||.||..:.      |..:|.|  .|...:|:.||.      |.|....::|.|.     ||:.
Mouse   661 ERLNFLRFLL------KDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLM-----GDEP 714

  Fly  1082 ALGIEALQHLYLKKLPELRPEEFSMVALGLFQQL-----CSFARIAMAEYDNHSDQISASASAVG 1141
            .|..:..:..:...:.:|.|...:...:..|::.     |...::.........|.:    ..:|
Mouse   715 DLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDL----ELIG 775

  Fly  1142 MYHLWKIALRAQSN-DVSLAAIQYINMYY--MGQQLR-----LEKEFVSQCMENLVQAATALESI 1198
            :.:||::.:  ||| |::..||..:...|  :|.:|:     :.::|:..|.:.|..:...|..:
Mouse   776 LDYLWRVVI--QSNDDIACRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVL 838

  Fly  1199 D-DENAL-------MRVQRGLLLLNTHLD-----------------TFRRRYAFHLRRWAIEGKG 1238
            | |::::       :|:.|.|.:|..:::                 .||.::...:.|:..:|:.
Mouse   839 DGDKDSINCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFIVRFPNQGRQ 903

  Fly  1239 IG-----SHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAP 1298
            :.     ||:   |:..|...|.:|.:                   :||.|:      .|.:...
Mouse   904 VDDLEVWSHT---NDTIGSVRRCILNR-------------------IKANVA------HTKIELF 940

  Fly  1299 VLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSL----------------GGRGA 1347
            |.|.|:..|.              |.:.:|....||..::...|                ...|:
Mouse   941 VGGELIDPGD--------------DRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGS 991

  Fly  1348 RRKESNLEHPSMLPPPPKE-CLPTVLL-LQPKYFEKLFCLMQTLGDMQPQASTVN-PQHHTKAQL 1409
            .....|  |.|..|.|..| |||.|:: |.|:|...|:    .:.|:   .|::| |.....|::
Mouse   992 PGNHGN--HYSDGPNPEVESCLPGVIMSLHPRYISFLW----QVADL---GSSLNMPPLRDGARV 1047

  Fly  1410 LSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQL-----ATKRKQIKQKFRDLLD 1469
            |       :.::|.:...::..:::..|.::|.:.......:.|     |::...:.:....||.
Mouse  1048 L-------MKLMPPDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYALLM 1105

  Fly  1470 P------NNLQKFMYSLHIVES----LALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNE 1524
            |      ::...|.:  |.::|    |.|:...|.....|..|          ::..|...|:
Mouse  1106 PAGAPLTDDSSDFQF--HFLKSGGLPLVLSMLTRNNFLPNADM----------ETRRGAYLNA-- 1156

  Fly  1525 QPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQ 1589
                    :|::|..|..:          |...|...|.:...|.:...|.....:    .|.:|
Mouse  1157 --------LKIAKLLLTAI----------GYGHVRAVAEACQPGVEGVNPMTSVNQ----VTHDQ 1199

  Fly  1590 EKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALN 1654
            .........:.|:|:                ||..::...:|...:|..|.    |.:..::.:
Mouse  1200 AVVLQSALQSIPNPS----------------SECMLRNVSVRLAQQISDEA----SRYMPDICV- 1243

  Fly  1655 EWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQH-- 1717
                  :.::.:|:|..|...||      |:..|:.      ||.:...:.::....::..:.
Mouse  1244 ------IRAIQKIIWTSGCGGLQ------LVFSPNE------EVTKIYEKTNAGNEPDLEDEQVC 1290

  Fly  1718 -QASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTD---HCRWL 1778
             :|.....|.|               .||..|::.|         ::...|.....|   ||.
Mouse  1291 CEALEVMTLCF---------------ALIPTALDAL---------SKEKAWQTFIIDLLLHCH-- 1329

  Fly  1779 QKFILEDPEPAVRRE----ICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQF 1839
            .|.:.:     |.:|    :|.   |.|:|  |...|....||..::..........||
Mouse  1330 SKTVRQ-----VAQEQFFLMCT---RCCMG--HRPLLFFITLLFTVLGSTARERAKHSG------ 1378

  Fly  1840 LLSEEGKDPYGPACRDYFWLLARL--------VDTLSPEMVAEEHID-IEMLCESISQSILTREY 1895
                           |||.||..|        ::..:.|::....|| ::.:.:.:.::..|
Mouse  1379 ---------------DYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGET--- 1425

  Fly  1896 YELRHGYQDDGLVGLLNLMSNLIKYDTTFK-----YTPKALSFIEQLI-GFLFDMPS-------- 1946
                 |.::..|.|.|.:...|:.:.|..|     ......:.|::|| .|:|...:
Mouse  1426 -----GVEETILEGHLGVTKELLAFQTPEKKFHIGCEKGGANLIKELIDDFIFPASNVYLQYMRN 1485

  Fly  1947 ---PADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGP-----KQPYPWDYW 2003
               ||::..|.|.|.::..:.::|||.|..||..|...:...|...:..|.     :....|:|.
Mouse  1486 GELPAEQAIPVCGSPATINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYL 1550

  Fly  2004 PRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRV---------------------- 2046
            |....|...|:|||.|.||||||.|.:|.|||:|..|..:|.:
Mouse  1551 PPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESN 1615

  Fly  2047 -----------------PP---NAARKH---GPTLLELQRMFAYLLESERKSYNPRSFCRVYQMD 2088
                             ||   ...||.   | .|..||.:|.:|..|..:.|.||.|.:.:::.
Mouse  1616 VDPRDDVFGYPQQFEDKPPLSKTEDRKEYNIG-VLRHLQVIFGHLAASRLQYYVPRGFWKQFRLW 1679

  Fly  2089 HQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKH--LVKRLFCGSLSNNVVSLDCGHVSRTAEDFYT 2151
            .:|:|..||.|..|||..||..|::....|.|  ::.::..||.::..:...|.|.....|.|.|
Mouse  1680 GEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTT 1744

  Fly  2152 VRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNM 2216
            :...:.:.:||.:||::....|.|||.|.|.|.:|.|||...||...||||.:|.....|:.::.
Mouse  1745 LNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDW 1809

  Fly  2217 VTMLKEKVNTHFSFPLRLNMCHY----VEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEA 2277
            ......|.|.:|.||..|:|..|    |.|........|.:..:|.|:         :::|||.:
Mouse  1810 ERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQNEQ---------SESEKAGS 1865

  Fly  2278 TLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEV---KPFDPSQ 2339
            |         :|.||||.||:|.|.||||||:|.:|.......: ||:.|:|.:|   |..|..:
Mouse  1866 T---------KYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKN-RWYKFDDGDVTECKMDDDEE 1920

  Fly  2340 IAAECFGGEMTSKTYDSVTEKYLDFSFEKTN---SAYMLFYERR----LPEHLQRRHSELLVTPT 2397
            :..:|||||...:.:|.:.::   .|:.:..   :||:|||||.    ..:.:.|..||:.:|..
Mouse  1921 MKNQCFGGEYMGEVFDHMMKR---MSYRRQKRWWNAYILFYERMDTIGHDDEVIRYISEIAITTR 1982

  Fly  2398 PSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQ 2462
            |...|       .|:.:|.|
Mouse  1983 PHQIV-------MPSAIERS--------------------------------------------- 1995

  Fly  2463 DEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTS 2527
                   :.||
Mouse  1996 -------VRKQ------------------------------------------------------ 1999

  Fly  2528 KAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQI--CGHI------------P 2578
                                    |.||:.:|..:...||.||.::  |..:            |
Mouse  2000 ------------------------NVQFMHNRMQYSLEYFQFMKKLLTCNGVYLNPPPGQDHLSP 2040

  Fly  2579 QSLISETDVTCMAAKLSVSFFIETFIHAKE--KPTMVPWVELLTKQFNASQEACEWF-------L 2634
            ::    .::|.::.:|:..|...|..|.|:  :.:...|.:.|......|:....||       :
Mouse  2041 EA----EEITMISIQLAARFLFTTGFHTKKIVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNV 2101

  Fly  2635 SHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLE-------VETDEDDKELI 2692
            |:...|      .|::||:..||..|.:|::.:        ||..|:       ..:.....:..
Mouse  2102 SNRFSE------YLLECPSAEVRGAFAKLIVFI--------AHFSLQDGPCPSPFASPGPSSQAY 2152

  Fly  2693 GQASCVTRFIGSLISLL-----EHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVD 2752
            ...|.....:.::::||     |||     |||.:||.|...::.:|..|...||::.|..:.:.
Mouse  2153 DNLSLSDHLLRAVLNLLRREVSEHG-----RHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFML 2212

  Fly  2753 FYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFS 2817
            ..|.......|                  |.:.|.|.|:.::.:.|: |......|::.|.....
Mouse  2213 VSLDEGPGPPI------------------KYQYAELGKLYSVVSQLI-RCCNVSSRMQSSINGNP 2258

  Fly  2818 AIAGGKGFPFLYQQ----------------------IKDGINPHQTKHLIHALCRWDERLATQII 2860
            ::....|.|.|.|.                      |:|..|..:|..|:...|..:.:.::.::
Mouse  2259 SLPNPFGDPNLSQPIMPIQQNVVDILFVRTSYVKKIIEDCSNSDETVKLLRFCCWENPQFSSTVL 2323

  Fly  2861 GMLFASVT-KHTELCAPFFKLL--TLLTE 2886
            ..|...|. .:|....|:..||  .||.|
Mouse  2324 SELLWQVAYSYTYELRPYLDLLLQILLIE 2352

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
puf	NP_001262923.1	peptidase_C19C	2013..2382	CDD:239124	132/429 (31%)
		UCH	2014..2377	CDD:278850	127/419 (30%)
		DUF3517	2723..>2877	CDD:288853	31/176 (18%)
Usp9x	XP_006527649.1	Ubiquitin_like_fold	<905..969	CDD:391949	18/105 (17%)
		peptidase_C19C	1560..1963	CDD:239124	132/425 (31%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5077
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D625455at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.910